Phenotypic properties of a unique rpoA mutation (phs) of Escherichia coli.

abstract：:The genes for protein synthesis elongation factors Tu and G were cloned from the cyanobacterium Anacystis nidulans. The locations of these genes were mapped within the cloned DNA fragment by hybridization with Escherichia coli probes. The organization of the cloned fragment and the DNA flanking it in the A. nidulans c...

journal_title：Journal of bacteriology

authors： Mickel FS,Spremulli LL

更新日期：1986-04-01 00:00:00

abstract：:Flagellar biogenesis and hence motility of Vibrio fischeri depends upon the presence of magnesium. In the absence of magnesium, cells contain few or no flagella and are poorly motile or nonmotile. To dissect the mechanism by which this regulation occurs, we screened transposon insertion mutants for those that could mi...

journal_title：Journal of bacteriology

authors： O'Shea TM,Klein AH,Geszvain K,Wolfe AJ,Visick KL

更新日期：2006-12-01 00:00:00

abstract：:Narkates, Annie J. (Southern Research Institute, Birmingham, Ala.), and Robert F. Pittillo. Influence of inoculum media on sensitivity of Escherichia coli to azaserine. J. Bacteriol. 90:710-714. 1965.-Escherichia coli grown in a simple synthetic medium is more than 100-fold more sensitive to inhibition by azaserine th...

journal_title：Journal of bacteriology

authors： Narkates AJ,Pittillo RF

更新日期：1965-09-01 00:00:00

abstract：:The composition of the Pseudomonas solanacearum lipolysaccharide (LPS) was found to be similar to that described for the LPS of enterobacteria. The lipid A contained fatty acids and glucosamine in a molar ratio of 5:2. The LPS fraction contained 2-keto-3-deoxyoctulosonic acid, L-glycero-D-mannoheptose, hexoses (glucos...

journal_title：Journal of bacteriology

authors： Drigues P,Demery-Lafforgue D,Trigalet A,Dupin P,Samain D,Asselineau J

更新日期：1985-05-01 00:00:00

abstract：:The interaction of Streptococcus pyogenes (group A streptococcus [GAS]) with its human host requires several surface proteins. In this study, we isolated mutations in a gene required for the surface localization of protein F by transposon mutagenesis of the M6 strain JRS4. This gene (srtA) encodes a protein homologous...

journal_title：Journal of bacteriology

authors： Barnett TC,Scott JR

更新日期：2002-04-01 00:00:00

abstract：:Jones, Lois M. (University of Wisconsin, Madison), C. R. McDuff, and J. B. Wilson. Phenotypic alterations in the colonial morphology of Brucella abortus due to a bacteriophage carrier state. J. Bacteriol. 83:860-866. 1962.-In the course of examining a number of Brucella cultures with a brucellaphage, it was observed t...

journal_title：Journal of bacteriology

authors： JONES LM,McDUFF CR,WILSON JB

更新日期：1962-04-01 00:00:00

abstract：:A key step in the regulation of heat shock genes in Escherichia coli is the stress-dependent degradation of the heat shock promoter-specific sigma(32) subunit of RNA polymerase by the AAA protease, FtsH. Previous studies implicated the C termini of protein substrates, including sigma(32), as degradation signals for AA...

journal_title：Journal of bacteriology

authors： Tomoyasu T,Arsène F,Ogura T,Bukau B

更新日期：2001-10-01 00:00:00

abstract：:An increased demand for cytochromes is associated with symbiotic development and microaerobic metabolism in the bacterium Bradyrhizobium japonicum, and evidence suggests that hemB, rather than hemA, is the first essential bacterial heme synthesis gene in symbiosis with soybean. Steady-state levels of mRNA and protein ...

journal_title：Journal of bacteriology

authors： Chauhan S,O'Brian MR

更新日期：1997-06-01 00:00:00

abstract：:d-Desthiobiotin synthetase, an enzyme that catalyzes the synthesis of d-desthiobiotin from dl-7,8-diaminopelargonic acid and HCO(3) (-), was purified 100-fold from cells of a biotin mutant strain of Escherichia coli. Adenosine triphosphate and Mg(2+) were shown, especially in purified extracts, to be obligatory for en...

journal_title：Journal of bacteriology

authors： Cheeseman P,Pai CH

更新日期：1970-11-01 00:00:00

abstract：:Trent, Dennis W. (University of Oklahoma School of Medicine, Oklahoma City) and L. Vernon Scott. Colorado tick fever virus in cell culture. I. Cell-type susceptibility and interaction with L cells. J. Bacteriol. 88:702-708. 1964.-Colorado tick fever (CTF) virus was serially propagated in monolayer cultures of L and FL...

journal_title：Journal of bacteriology

authors： TRENT DW,SCOTT LV

更新日期：1964-09-01 00:00:00

abstract：:To examine as randomly as possible the role of the beta-ketoacyl and acyl carrier protein (ACP) components of bacterial type II polyketide synthases (PKSs), homologs of the chain-length-factor (CLF) genes were cloned from the environmental community of microorganisms. With PCR primers derived from conserved regions of...

journal_title：Journal of bacteriology

authors： Seow KT,Meurer G,Gerlitz M,Wendt-Pienkowski E,Hutchinson CR,Davies J

更新日期：1997-12-01 00:00:00

abstract：:The SecYEG heterotrimeric membrane protein complex functions as a channel for protein translocation across the Escherichia coli cytoplasmic membrane. SecY is the central subunit of the SecYEG complex and contains 10 transmembrane segments (TM1 to TM10). Previous mutation studies suggested that TM3 and TM4 are particul...

journal_title：Journal of bacteriology

authors： Mori H,Shimokawa N,Satoh Y,Ito K

更新日期：2004-06-01 00:00:00

abstract：:Transposon mutagenesis was used to isolate mutants of Aeromonas hydrophila which were deficient in the production of extracellular proteins. The culture supernatants of two of the mutants were essentially devoid of the proteins normally secreted by the parent strain, despite their continued synthesis. Western immunobl...

journal_title：Journal of bacteriology

authors： Bo JN,Howard SP

更新日期：1991-02-01 00:00:00

abstract：:The Escherichia coli zupT (formerly ygiE) gene encodes a cytoplasmic membrane protein (ZupT) related to members of the eukaryotic ZIP family of divalent metal ion transporters. Previously, ZupT was shown to be responsible for uptake of zinc. In this study, we show that ZupT is a divalent metal cation transporter of br...

journal_title：Journal of bacteriology

authors： Grass G,Franke S,Taudte N,Nies DH,Kucharski LM,Maguire ME,Rensing C

更新日期：2005-03-01 00:00:00

abstract：:Thermococcus sp. strain 4557 is a hyperthermophilic anaerobic archaeon isolated from the deep-sea hydrothermal vent Guaymas Basin site in the Gulf of California at a depth of 2,000 m. Here, we present the complete genome sequence of Thermococcus sp. 4557, which consists of a single circular chromosome of 2,011,320 bp ...

journal_title：Journal of bacteriology

authors： Wang X,Gao Z,Xu X,Ruan L

更新日期：2011-10-01 00:00:00

abstract：:The azlB locus of Bacillus subtilis was defined previously by a mutation conferring resistance to a leucine analog, 4-azaleucine (J. B. Ward, Jr., and S. A. Zahler, J. Bacteriol. 116:727-735, 1973). In this report, azlB is shown to be the first gene of an operon apparently involved in branched-chain amino acid transpo...

journal_title：Journal of bacteriology

authors： Belitsky BR,Gustafsson MC,Sonenshein AL,Von Wachenfeldt C

更新日期：1997-09-01 00:00:00

abstract：:Members of the Streptococcus bovis group are important causes of endocarditis. However, factors associated with their pathogenicity, such as adhesins, remain uncharacterized. We recently demonstrated that endocarditis-derived Streptococcus gallolyticus subsp. gallolyticus isolates frequently adhere to extracellular ma...

journal_title：Journal of bacteriology

authors： Sillanpää J,Nallapareddy SR,Qin X,Singh KV,Muzny DM,Kovar CL,Nazareth LV,Gibbs RA,Ferraro MJ,Steckelberg JM,Weinstock GM,Murray BE

更新日期：2009-11-01 00:00:00

abstract：:A 5.4-kilobase DNA fragment carrying Pseudomonas denitrificans cob genes has been sequenced. The nucleotide sequence and genetic analysis revealed that this fragment carries five different cob genes (cobA to cobE). Four of these genes present the characteristics of translationally coupled genes. cobA has been identifi...

journal_title：Journal of bacteriology

authors： Crouzet J,Cauchois L,Blanche F,Debussche L,Thibaut D,Rouyez MC,Rigault S,Mayaux JF,Cameron B

更新日期：1990-10-01 00:00:00

abstract：:FtsK is essential for Escherichia coli cell division. We report that cells lacking the C terminus of FtsK are defective in chromosome segregation as well as septation, often exhibiting asymmetrically positioned nucleoids and large anucleate regions. Combining the corresponding truncated ftsK gene with a mukB null muta...

journal_title：Journal of bacteriology

authors： Yu XC,Weihe EK,Margolin W

更新日期：1998-12-01 00:00:00

abstract：:Mutants of the fhuA gene of Escherichia coli K-12, which encodes a receptor protein in the outer membrane, took up ferrichrome after exposure to pronase, whereas fhuB mutants remained transport negative. The latter finding supports our previous proposal that fhuB mutants are defective in a function that residues in th...

journal_title：Journal of bacteriology

authors： Wookey PJ,Hussein S,Braun V

更新日期：1981-06-01 00:00:00

abstract：:The tyrT (tRNA(1TYr)) locus of Escherichia coli B differs structurally from that of K-12 strains by the absence of 2 of 3.14 terminal repeat sequences. ...

journal_title：Journal of bacteriology

authors： Timms AR,Bridges BA

更新日期：1996-04-01 00:00:00

abstract：:The interruption of the sblA gene of Streptomyces lividans was previously shown to lead to relief of glucose repression of the normally strongly glucose-repressed alpha-amylase gene. In addition to this relief, an early entry into stationary phase was observed when cells were grown in a minimal medium containing gluco...

journal_title：Journal of bacteriology

authors： Chouayekh H,Nothaft H,Delaunay S,Linder M,Payrastre B,Seghezzi N,Titgemeyer F,Virolle MJ

更新日期：2007-02-01 00:00:00

abstract：:The physical arrangement of cell envelope components leads to the exposure of selected structural motifs which in turn may influence host-parasite interactions. To gain insight into the exposed epitopes, the present study describes a flow cytometric method designed to probe defined molecules on dispersed mycobacteria....

journal_title：Journal of bacteriology

authors： Ozanne V,Ortalo-Magne A,Vercellone A,Fournie JJ,Daffe M

更新日期：1996-12-01 00:00:00

abstract：:The phycocyanin subunit gene cluster is duplicated on the chromosome of the cyanobacterium Anacystis nidulans UTEX 625. The two gene clusters cpcB1A1 (left) and cpcB2A2 (right) are separated by about 2,500 base pairs, and in each cluster the beta-subunit gene is located upstream from the alpha-subunit gene. Filter hyb...

journal_title：Journal of bacteriology

authors： Kalla SR,Lind LK,Lidholm J,Gustafsson P

更新日期：1988-07-01 00:00:00

abstract：:For the transformation of the yeast species Kluyveromyces fragilis, we have constructed a vector containing a bacterial kanamycin resistance (Kmr) gene, the TRP1 gene of Saccharomyces cerevisiae, and an autonomously replicating sequence of Kluyveromyces lactis called KARS2 . By utilizing the method based on treatment ...

journal_title：Journal of bacteriology

authors： Das S,Kellermann E,Hollenberg CP

更新日期：1984-06-01 00:00:00

abstract：:A search by subtractive hybridization for sequences present in only certain strains of Helicobacter pylori led to the discovery of a 2-kb transposable element to be called IS607, which further PCR and hybridization tests indicated was present in about one-fifth of H. pylori strains worldwide. IS607 contained two open ...

journal_title：Journal of bacteriology

authors： Kersulyte D,Mukhopadhyay AK,Shirai M,Nakazawa T,Berg DE

更新日期：2000-10-01 00:00:00

abstract：:The cell-wall-less prokaryote Mycoplasma pneumoniae, long considered among the smallest and simplest cells capable of self-replication, has a distinct cellular polarity characterized by the presence of a differentiated terminal organelle which functions in adherence to human respiratory epithelium, gliding motility, a...

journal_title：Journal of bacteriology

authors： Hasselbring BM,Jordan JL,Krause DC

更新日期：2005-09-01 00:00:00

abstract：:The colH gene encoding a collagenase was cloned from Clostridium histolyticum JCM 1403. Nucleotide sequencing showed a major open reading frame encoding a 116-kDa protein of 1,021 amino acid residues. The deduced amino acid sequence contains a putative signal sequence and a zinc metalloprotease consensus sequence, HEX...

journal_title：Journal of bacteriology

authors： Yoshihara K,Matsushita O,Minami J,Okabe A

更新日期：1994-11-01 00:00:00

abstract：:Outer membrane alterations were characterized in spontaneous mutants of the Erwinia chrysanthemi 3937jRH, which were selected for resistance to bacteriophage phi EC2. All but one of the mutants analyzed were affected in their lipopolysaccharide (LPS) structure, lacking the entire heterogeneous region of apparent high ...

journal_title：Journal of bacteriology

authors： Schoonejans E,Expert D,Toussaint A

更新日期：1987-09-01 00:00:00

abstract：:The tpr gene family of Treponema pallidum subsp. pallidum, the causative agent of syphilis, has recently become the focus of intensive investigation. TprF and TprI sequences are highly conserved among different isolates and are the targets of strong humoral and cellular immune responses of the host, and immunization w...

journal_title：Journal of bacteriology

authors： Giacani L,Hevner K,Centurion-Lara A

更新日期：2005-09-01 00:00:00