Comparative studies of lipopolysaccharide and exopolysaccharide from a virulent strain of Pseudomonas solanacearum and from three avirulent mutants.

abstract：:Shiga toxin (STX), a bacterial toxin produced by Shigella dysenteriae type 1, is a hexamer composed of five receptor-binding B subunits which encircle an alpha-helix at the carboxyl terminus of the enzymatic A polypeptide. Hybrid toxins constructed by fusing the A polypeptide sequences of STX and Shiga-like toxin type...

journal_title：Journal of bacteriology

authors： Jemal C,Haddad JE,Begum D,Jackson MP

更新日期：1995-06-01 00:00:00

abstract：:Escherichia coli VC30 is a temperature-sensitive mutant which is defective in autolysis. Strain VC30 lyses at 30 degrees C when treated with beta-lactam antibiotics or D-cycloserine or when deprived of diaminiopimelic acid. The same treatments inhibit growth of the mutant at 42 degrees C but do not cause lysis. Strain...

journal_title：Journal of bacteriology

authors： Lubitz W,Harkness RE,Ishiguro EE

更新日期：1984-07-01 00:00:00

abstract：:Motility in mycobacteria was described for the first time in 1999. It was reported that Mycobacterium smegmatis and Mycobacterium avium could spread on the surface of solid growth medium by a sliding mechanism and that the presence of cell wall glycopeptidolipids was essential for motility. We recently reported that M...

journal_title：Journal of bacteriology

authors： Agustí G,Astola O,Rodríguez-Güell E,Julián E,Luquin M

更新日期：2008-10-01 00:00:00

abstract：:We have devised a coupled radiochemical assay for detecting phosphatidylglycerolphosphate (PGP) phosphatase activity in Escherichia coli colonies immobilized on filter paper. There appeared to be at least two enzymes capable of dephosphorylating PGP, as judged by the characterization of mutations in two genes designat...

journal_title：Journal of bacteriology

authors： Icho T,Raetz CR

更新日期：1983-02-01 00:00:00

abstract：:Undecyl acetate esterase has been purified from Pseudomonas cepacia grown on the methyl ketone, 2-tridecanone. The K(m) for undecyl acetate was 2.3 x 10(-2) M. Polyacrylamide gel electrophoresis indicated that two esterase bands were being recovered during purification. These bands were separated by preparative polyac...

journal_title：Journal of bacteriology

authors： Shum AC,Markovetz AJ

更新日期：1974-06-01 00:00:00

abstract：:Two hundred strains of Escherichia coli harboring Filv+ plasmids which carry a segment of the Salmonella typhimurium chromosome were isolated independently. Among them, two strains were found to harbor F' plasmids that are able to replicate in Hfr cells of E. coli; i.e., they carry a site designated poh (permissive on...

journal_title：Journal of bacteriology

authors： Joh K,Hiraga S

更新日期：1979-05-01 00:00:00

abstract：:Azotobacter vinelandii was grown diazotrophically in chemostat cultures limited by sucrose, citrate, or acetate. Specific activities of cellular oxygen consumption (qO2) and nitrogenase (acetylene reduction) were measured in situ at different dilution rates (D, representing the specific growth rate mu at steady state)...

journal_title：Journal of bacteriology

authors： Kuhla J,Oelze J

更新日期：1988-11-01 00:00:00

abstract：:Despite a large number of studies on the role of oxygen in cellular processes, there is no consensus as to how oxygen availability to the cell should be defined, let alone how it should be quantified. Here, a quantitative definition for oxygen availability (perceived aerobiosis) is presented; the definition is based o...

journal_title：Journal of bacteriology

authors： Alexeeva S,Hellingwerf KJ,Teixeira de Mattos MJ

更新日期：2002-03-01 00:00:00

abstract：:A gene (yacK) encoding a putative multicopper oxidase (MCO) was cloned from Escherichia coli, and the expressed enzyme was demonstrated to exhibit phenoloxidase and ferroxidase activities. The purified protein contained six copper atoms per polypeptide chain and displayed optical and electron paramagnetic resonance (E...

journal_title：Journal of bacteriology

authors： Kim C,Lorenz WW,Hoopes JT,Dean JF

更新日期：2001-08-01 00:00:00

abstract：UNLABELLED:During biosynthesis of [NiFe]-hydrogenase 2 (Hyd-2) of Escherichia coli, a 15-amino-acid C-terminal peptide is cleaved from the catalytic large subunit precursor, pro-HybC. This peptide is removed only after NiFe(CN)2CO cofactor insertion by the Hyp accessory protein machinery has been completed, suggesting ...

journal_title：Journal of bacteriology

authors： Thomas C,Muhr E,Sawers RG

更新日期：2015-09-01 00:00:00

abstract：:In many organisms, phosphatase expression and phosphate (P) uptake are coordinately regulated by the Pho regulon. In Myxococcus xanthus P limitation initiates multicellular development, a process associated with changes in phosphatase expression. We sought here to characterize the link between P acquisition and develo...

journal_title：Journal of bacteriology

authors： Whitworth DE,Holmes AB,Irvine AG,Hodgson DA,Scanlan DJ

更新日期：2008-03-01 00:00:00

abstract：:Deletions in the tet genes derived from Tn10 were formed from different tet::Tn5 insertion mutations by removing DNA sequences located between a HindIII site in Tn5 and a HindIII site adjacent to the tet genes. Tetracycline-sensitive point mutations were mapped in recombination tests with the deletions and were thus a...

journal_title：Journal of bacteriology

authors： Coleman DC,Chopra I,Shales SW,Howe TG,Foster TJ

更新日期：1983-02-01 00:00:00

abstract：:Penicillin-binding protein (PBP) 7 of Escherichia coli is a poorly characterized member of the family of enzymes that synthesize and modify the bacterial cell wall. The approximate chromosomal position of the gene encoding this protein was determined by measuring the expression of PBPs during lytic infection of E. col...

journal_title：Journal of bacteriology

authors： Henderson TA,Templin M,Young KD

更新日期：1995-04-01 00:00:00

abstract：:A cosmid vector has been constructed, using a lambda replicon. A library of cosmids from Anabaena variabilis ATCC 29413 based on use of this vector is shown to be highly deficient in sites for the two type II restriction endonucleases found in that organism. ...

journal_title：Journal of bacteriology

authors： Herrero A,Elhai J,Hohn B,Wolk CP

更新日期：1984-11-01 00:00:00

abstract：:Gaines, Sidney (Walter Reed Army Institute of Research, Washington, D.C.), Julius A. Currie, and Joseph G. Tully. Factors affecting formation of incomplete Vi antibody in mice. J. Bacteriol. 90:635-642. 1965.-Single immunizing doses of purified Vi antigen elicited complete and incomplete Vi antibodies in BALB/c mice, ...

journal_title：Journal of bacteriology

authors： Gaines S,Currie JA,Tully JG

更新日期：1965-09-01 00:00:00

abstract：:Using physical and genetic data, we have demonstrated that Rhizobium meliloti SU47 has a symbiotic megaplasmid, pRmeSU47b, in addition to the previously described nod-nif megaplasmid pRmeSU47a. This plasmid includes four loci involved in exopolysaccharide (exo) synthesis as well as two loci involved in thiamine biosyn...

journal_title：Journal of bacteriology

authors： Finan TM,Kunkel B,De Vos GF,Signer ER

更新日期：1986-07-01 00:00:00

abstract：:We report the complete genome sequence of Helicobacter cinaedi strain PAGU611, isolated in a case of human bacteremia. The PAGU611 genome comprises a 2,078,348-bp chromosome and a 23,054-bp plasmid. The chromosome contains a unique genomic island, encoding a type VI secretion system and clustered regularly interspaced...

journal_title：Journal of bacteriology

authors： Goto T,Ogura Y,Hirakawa H,Tomida J,Morita Y,Akaike T,Hayashi T,Kawamura Y

更新日期：2012-07-01 00:00:00

abstract：:Rickettsia typhi, the causative agent of murine typhus, is an obligate intracellular bacterium with a life cycle involving both vertebrate and invertebrate hosts. Here we present the complete genome sequence of R. typhi (1,111,496 bp) and compare it to the two published rickettsial genome sequences: R. prowazekii and ...

journal_title：Journal of bacteriology

authors： McLeod MP,Qin X,Karpathy SE,Gioia J,Highlander SK,Fox GE,McNeill TZ,Jiang H,Muzny D,Jacob LS,Hawes AC,Sodergren E,Gill R,Hume J,Morgan M,Fan G,Amin AG,Gibbs RA,Hong C,Yu XJ,Walker DH,Weinstock GM

更新日期：2004-09-01 00:00:00

abstract：:The rnt gene encoding RNase T was cloned on a 13-kilobase BamHI fragment. Restriction analysis of the fragment and comparison of it with the Escherichia coli restriction map localized rnt to kilobase coordinates 1733 to 1746, corresponding to about 36 min on the genetic map. The map location was confirmed by cotransdu...

journal_title：Journal of bacteriology

authors： Case LM,Chen XN,Deutscher MP

更新日期：1989-10-01 00:00:00

abstract：:Most mutations in the yajC-secDF operon identified via genetic screens confer a cold-sensitive growth phenotype. Here we report that two of these mutations confer this cold-sensitive phenotype by inactivating the SecDF-YajC complex in protein translocation. ...

journal_title：Journal of bacteriology

authors： Nouwen N,Driessen AJ

更新日期：2005-10-01 00:00:00

abstract：UNLABELLED:TcpP and ToxR coordinately regulate transcription of toxT, the master regulator of numerous virulence factors in Vibrio cholerae. TcpP and ToxR are membrane-localized transcription factors, each with a periplasmic domain containing two cysteines. In ToxR, these cysteines form an intramolecular disulfide bond...

journal_title：Journal of bacteriology

authors： Morgan SJ,French EL,Thomson JJ,Seaborn CP,Shively CA,Krukonis ES

更新日期：2015-11-16 00:00:00

abstract：:Type IV secretion systems (T4SS) mediate the transfer of DNA and protein substrates to target cells. TrwK, encoded by the conjugative plasmid R388, is a member of the VirB4 family, comprising the largest and most conserved proteins of T4SS. VirB4 was suggested to be an ATPase involved in energizing pilus assembly and ...

journal_title：Journal of bacteriology

authors： Arechaga I,Peña A,Zunzunegui S,del Carmen Fernández-Alonso M,Rivas G,de la Cruz F

更新日期：2008-08-01 00:00:00

abstract：:The genome of Corynebacterium glutamicum type strain ATCC 13032 (accession number BX927147) contains three prophages, CGP1, CGP2, and CGP3. We recently observed that many genes within the CGP3 prophage region have increased mRNA levels in a dtxR deletion mutant that lacks the master regulator of iron homeostasis (J. W...

journal_title：Journal of bacteriology

authors： Frunzke J,Bramkamp M,Schweitzer JE,Bott M

更新日期：2008-07-01 00:00:00

abstract：:Phosphoenolpyruvate carboxylase (EC 4.1.1.31) from Azotobacter vinelandii, like the corresponding enzyme from other organisms, is activated by acetyl coenzyme A and inhibited by l-aspartate. Both modifiers affect primarily the affinity of the enzyme for phosphoenolpyruvate. This is the first enzyme with a strictly ana...

journal_title：Journal of bacteriology

authors： Liao CL,Atkinson DE

更新日期：1971-04-01 00:00:00

abstract：:A specialized transducing phage lambda carrying the structural gene for the OmpF protein, an outer membrane matrix protein, was isolated. The phage carries the 20.5--21-min region of the Escherichia coli K-12 chromosome and carries asnS, ompF, and aspC genes. ...

journal_title：Journal of bacteriology

authors： Mutoh N,Nagasawa T,Mizushima S

更新日期：1981-02-01 00:00:00

abstract：:Auxotrophic mutants of Hydrogenomonas eutropha and H. facilis requiring utilizable amino acids were employed to demonstrate the simultaneous utilization of H(2) and an organic substrate for growth. The ratio of the cell yields under dual substrate conditions compared to heterotrophic conditions indicated the relative ...

journal_title：Journal of bacteriology

authors： DeCicco BT,Stukus PE

更新日期：1968-04-01 00:00:00

abstract：:The DNA-binding factor BifA (previously called VF1) binds upstream of the developmentally regulated site-specific recombinase gene xisA in the cyanobacterium Anabaena sp. strain PCC 7120. Besides binding xisA, BifA also binds the glnA, rbcL, and nifH promoter regions. DNase I footprint analysis of BifA binding to glnA...

journal_title：Journal of bacteriology

authors： Ramasubramanian TS,Wei TF,Golden JW

更新日期：1994-03-01 00:00:00

abstract：:Tn2603 is a multiple-resistance transposon encoding resistance to ampicillin, streptomycin, sulfonamide, and mercury and having a molecular size of 20 kilobase pairs, with 200-base-pair inverted repeats at both ends. The essential sites and functions of Tn2603 which are required for its transposition were determined t...

journal_title：Journal of bacteriology

authors： Tanaka M,Harafuji H,Yamamoto T

更新日期：1982-08-01 00:00:00

abstract：:SigF is an alternative sigma factor that is highly conserved among species of the genus Mycobacterium. In this study we identified the SigF regulon in Mycobacterium smegmatis using whole-genome microarray and promoter consensus analyses. In total, 64 genes in exponential phase and 124 genes in stationary phase are Sig...

journal_title：Journal of bacteriology

authors： Hümpel A,Gebhard S,Cook GM,Berney M

更新日期：2010-05-01 00:00:00

abstract：:Three genes were shown to provide functions specific for ferric enterobactin transport in Escherichia coli: fepA encoded the outer membrane receptor, fepB produced a periplasmic protein, and the fepC product was presumably a component of a cytoplasmic membrane permease system for this siderophore. A 10.6-kilobase-pair...

journal_title：Journal of bacteriology

authors： Ozenberger BA,Nahlik MS,McIntosh MA

更新日期：1987-08-01 00:00:00