Participation of Escherichia coli heat shock proteins DnaJ, DnaK, and GrpE in P1 plasmid replication.

Abstract:

:Low-copy-number plasmids, such as P1 prophage and the fertility factor F, require a plasmid-encoded replication protein and several host products for replication. Stable maintenance also depends on active partitioning of plasmids into daughter cells. Mini-P1 par+ and par plasmids were found to be destabilized by mutations in the dnaJ, dnaK, and grpE genes of Escherichia coli. The transformation efficiency and stability of mini-F plasmids were also reduced in the mutant strains. These results indicate that heat shock proteins DnaJ, DnaK, and GrpE play roles in the replication of plasmid P1 and probably also in of F.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Tilly K,Yarmolinsky M

doi

10.1128/jb.171.11.6025-6029.1989

subject

Has Abstract

pub_date

1989-11-01 00:00:00

pages

6025-9

issue

11

eissn

0021-9193

issn

1098-5530

journal_volume

171

pub_type

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