Abstract:
:An extremely thermophilic bacterium, Thermus thermophilus HB8, is one of the model organisms for systems biology. Its genome consists of a chromosome (1.85 Mb), a megaplasmid (0.26 Mb) designated pTT27, and a plasmid (9.3 kb) designated pTT8, and the complete sequence is available. We show here that T. thermophilus is a polyploid organism, harboring multiple genomic copies in a cell. In the case of the HB8 strain, the copy number of the chromosome was estimated to be four or five, and the copy number of the pTT27 megaplasmid seemed to be equal to that of the chromosome. It has never been discussed whether T. thermophilus is haploid or polyploid. However, the finding that it is polyploid is not surprising, as Deinococcus radiodurans, an extremely radioresistant bacterium closely related to Thermus, is well known to be a polyploid organism. As is the case for D. radiodurans in the radiation environment, the polyploidy of T. thermophilus might allow for genomic DNA protection, maintenance, and repair at elevated growth temperatures. Polyploidy often complicates the recognition of an essential gene in T. thermophilus as a model organism for systems biology.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Ohtani N,Tomita M,Itaya Mdoi
10.1128/JB.00662-10subject
Has Abstractpub_date
2010-10-01 00:00:00pages
5499-505issue
20eissn
0021-9193issn
1098-5530pii
JB.00662-10journal_volume
192pub_type
杂志文章abstract::Deletions in the tet genes derived from Tn10 were formed from different tet::Tn5 insertion mutations by removing DNA sequences located between a HindIII site in Tn5 and a HindIII site adjacent to the tet genes. Tetracycline-sensitive point mutations were mapped in recombination tests with the deletions and were thus a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.153.2.921-929.1983
更新日期:1983-02-01 00:00:00
abstract::Bacillus anthracis Sterne cured of the pXO1 plasmid had enhanced secreted protease activity during the postexponential phase but no change in hemolytic or lecithinase activities. A zymogen profile revealed at least six proteases, including serine, metal, and perhaps cysteine types. There were similar amounts of protea...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.9.3133-3138.2005
更新日期:2005-05-01 00:00:00
abstract::Bacteroides fragilis V479-1 (also designated strain 92) has previously been shown to contain a conjugative plasmid, pBF4, that specifies resistance to clindamycin (Cc). A report of inducible tetracycline (Tc) resistance in this strain suggested that this phenotype was also plasmid associated (G. Privitera et al., Natu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.151.1.281-287.1982
更新日期:1982-07-01 00:00:00
abstract::In Escherichia coli, the phosphorylation and dephosphorylation of isocitrate dehydrogenase (IDH) are catalyzed by a bifunctional protein kinase/phosphatase. We have determined the nucleotide sequence of aceK, the gene encoding IDH kinase/phosphatase. This gene consists of a single open reading frame of 1,734 base pair...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.6.2763-2769.1988
更新日期:1988-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.102.3.767-773.1970
更新日期:1970-06-01 00:00:00
abstract::The composition of the Pseudomonas solanacearum lipolysaccharide (LPS) was found to be similar to that described for the LPS of enterobacteria. The lipid A contained fatty acids and glucosamine in a molar ratio of 5:2. The LPS fraction contained 2-keto-3-deoxyoctulosonic acid, L-glycero-D-mannoheptose, hexoses (glucos...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.162.2.504-509.1985
更新日期:1985-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00026-09
更新日期:2009-04-01 00:00:00
abstract::The quorum-sensing (QS) system present in the emerging nosocomial pathogen Stenotrophomonas maltophilia is based on the signaling molecule diffusible signal factor (DSF). Production and detection of DSF are governed by the rpf cluster, which encodes the synthase RpfF and the sensor RpfC, among other components. Despit...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01540-14
更新日期:2014-07-01 00:00:00
abstract::Genome comparisons have demonstrated that dramatic genetic change often underlies the emergence of new bacterial pathogens. Evolutionary analysis of Escherichia coli O157:H7, a pathogen that has emerged as a worldwide public health threat in the past two decades, has posited that this toxin-producing pathogen evolved ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.5.1783-1791.2005
更新日期:2005-03-01 00:00:00
abstract::The stator of the sodium-driven flagellar motor of Vibrio alginolyticus is a membrane protein complex composed of four PomA and two PomB subunits. PomB has a peptidoglycan-binding motif in the C-terminal region. In this study, four kinds of PomB deletions in the C terminus were constructed. None of the deletion protei...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.2.778-784.2005
更新日期:2005-01-01 00:00:00
abstract::We have identified an analog of SspB, the proteolytic adaptor for transfer-messenger RNA (tmRNA)-tagged proteins, in Caulobacter crescentus. C. crescentus SspB shares limited sequence similarity with Escherichia coli SspB but binds the tmRNA tag in vitro and is required for optimal proteolysis of tagged proteins in vi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01387-06
更新日期:2007-01-01 00:00:00
abstract::The dimorphic fungus Mucor racemosus was found to contain the cyclic nucleotide guanosine 3',5'-monophosphate (cGMP). Approximately equivalent amounts of the compound were found in ungerminated spores, yeastlike cells, and mycelia. Germinating spores contained severalfold higher amounts of cGMP than the other cell for...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.125.3.1226-1228.1976
更新日期:1976-03-01 00:00:00
abstract::Three evolutionarily distinct families of replicative DNA polymerases, designated polymerase B (Pol B), Pol C, and Pol D, have been identified. Members of the Pol B family are present in all three domains of life, whereas Pol C exists only in Bacteria and Pol D exists only in Archaea. Pol B enzymes replicate eukaryoti...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.02037-12
更新日期:2013-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.2.903-905.1991
更新日期:1991-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.1.38-44.2000
更新日期:2000-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.5.1534-1542.2003
更新日期:2003-03-01 00:00:00
abstract::CRM 228 (T. Uchida, A. M. Pappenheimer, and R. Greany, J. Biol. Chem. 248:3838-3844, 1973), a mutant form of diphtheria toxin which completely lacks ADP-ribosyltransferase activity, contains five amino acid substitutions. The two amino acid changes that fall within the A chain of the toxin (G79D and E162K) were separa...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.15.4766-4769.1994
更新日期:1994-08-01 00:00:00
abstract::A Flavobacterium sp. (strain 50001), capable of degrading 2,4-dichlorophenoxyacetate (2,4-D), 2-methyl-4-chlorophenoxyacetate, and 2-chlorobenzoate and imparting resistance to mercury, harbored a degradative plasmid, pRC10. Cured strains of the Flavobacterium sp. lost the plasmid as well as the ability to degrade thes...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.9.3897-3902.1988
更新日期:1988-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.125.3.1032-1039.1976
更新日期:1976-03-01 00:00:00
abstract::Bacteria of the genus Prosthecobacter express homologs of eukaryotic α- and β-tubulin, called BtubA and BtubB (BtubA/B), that have been observed to assemble into filaments in the presence of GTP. BtubA/B polymers are proposed to be composed in vitro by two to six protofilaments in contrast to that in vivo, where they ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00211-17
更新日期:2017-09-05 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.148.3.1006-1011.1981
更新日期:1981-12-01 00:00:00
abstract::Lactobacillus pentosus MP-10 was isolated from brines of naturally fermented Aloreña green table olives. MP-10 has potential probiotic traits, including inhibition of human pathogenic bacteria, survival at low pH (1.5), and bile salt tolerance (3%). Here, we report for the first time the annotated genome sequence of L...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.05171-11
更新日期:2011-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.05163-11
更新日期:2011-12-01 00:00:00
abstract::The adherence of Ruminococcus albus 8 to crystalline cellulose was studied, and an affinity-based assay was also used to identify candidate cellulose-binding protein(s). Bacterial adherence in cellulose-binding assays was significantly increased by the inclusion of either ruminal fluid or micromolar concentrations of ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.22.5921-5927.1998
更新日期:1998-11-01 00:00:00
abstract::Sortases are transamidases that covalently link proteins to the peptidoglycan of gram-positive bacteria. The genome of the pathogenic bacterium Listeria monocytogenes encodes two sortases genes, srtA and srtB. The srtA gene product anchors internalin and some other LPXTG-containing proteins to the listerial surface. H...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.186.7.1972-1982.2004
更新日期:2004-04-01 00:00:00
abstract::Krebs cycle enzyme activity in Bacillus subtilis was examined under aerobic and anaerobic conditions. Citrate synthase and aconitase activities in cells grown anaerobically in the presence of nitrate were reduced by as much as 10- and 30-fold, respectively, from levels observed under aerobic culture conditions. The ma...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.13.3304-3311.1998
更新日期:1998-07-01 00:00:00
abstract::Bacterial pathogenesis depends on changes in metabolic and virulence gene expression in response to changes within a pathogen's environment. The plague-causing pathogen, Yersinia pestis, requires expression of the gene encoding the Pla protease for progression of pneumonic plague. The catabolite repressor protein Crp,...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00737-17
更新日期:2018-05-09 00:00:00
abstract::The effect of CheY and fumarate on switching frequency and rotational bias of the bacterial flagellar motor was analyzed by computer-aided tracking of tethered Escherichia coli. Plots of cells overexpressing CheY in a gutted background showed a bell-shaped correlation curve of Switching frequency and bias centering at...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.13.3375-3380.1998
更新日期:1998-07-01 00:00:00
abstract::Features of the amino acid sequence of the TnaC nascent peptide are recognized by the translating ribosome. Recognition leads to tryptophan binding within the translating ribosome, inhibiting the termination of tnaC translation and preventing Rho-dependent transcription termination in the tna operon leader region. It ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01869-06
更新日期:2007-04-01 00:00:00
abstract::Bacillus licheniformis strain 749/C (constitutive for penicillinase formation) and uninduced cells of strain 749 (penicillinase-inducible) were examined after freezeetching. In the early stationary phase, strain 749/C organisms had clusters of vesicles (30 to 40 nm in diameter) on the outer surface of the plasma membr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.100.2.1002-1009.1969
更新日期:1969-11-01 00:00:00