Light-dependent regulation of cyanobacterial phytochrome expression.

abstract：:Purified Rickettsia prowazekii cells were able to transport L-proline. The influx of this amino acid had a Kt of 14 microM and a Vmax of about 64 pmol/min per mg of protein. Proline could not be transported by heat-killed or metabolically poisoned rickettsiae or at 0 degrees C. The uptake of proline was linear for alm...

journal_title：Journal of bacteriology

authors： Winkler HH,Daugherty RM

更新日期：1984-05-01 00:00:00

abstract：:During nitrogen-limited growth, transcription of glnA, which codes for glutamine synthetase, requires sigma 54-RNA polymerase and the phosphorylated from the nitrogen regulator I (NRI; also called NtrC). In cells in which the lac promoter controlled expression of the gene coding for NRI, increasing the intracellular c...

journal_title：Journal of bacteriology

authors： Shiau SP,Schneider BL,Gu W,Reitzer LJ

更新日期：1992-01-01 00:00:00

abstract：:Despite extensive similarities between the genomes of the Streptomyces temperate phages phiC31 and phiBT1, the attP-int loci are poorly conserved. Here we demonstrate that phiBT1 integrates into a different attachment site than phiC31. phiBT1 attB lies within SCO4848 encoding a 79-amino-acid putative integral membrane...

journal_title：Journal of bacteriology

authors： Gregory MA,Till R,Smith MC

更新日期：2003-09-01 00:00:00

abstract：:Six mycoplasma strains, isolated under anaerobic conditions from the human oropharynx, were studied by biologic and serologic means. The strains produced nippled colonies with weak hemolytic activity for guinea pig erythrocytes on agar medium. In addition, the strains metabolized arginine with a concomitant alkaline s...

journal_title：Journal of bacteriology

authors： Fox H,Purcell RH,Chanock RM

更新日期：1969-04-01 00:00:00

abstract：:The effect of the ilvG671, ilvG468, and ilvG603 mutations (phenotype, IlvG+ Valr; formerly ilvO) upon proteins synthesized was determined by infection of irradiated Escherichia coli K-12 cells, using specifically constructed derivatives of lambda dilv phage. These ilvG alleles are similar to the previously studied ilv...

journal_title：Journal of bacteriology

authors： Gray JE,Wallen JW,Calhoun DH

更新日期：1982-07-01 00:00:00

abstract：:MurG is a peripheral membrane protein that is one of the key enzymes in peptidoglycan biosynthesis. The crystal structure of Escherichia coli MurG (S. Ha, D. Walker, Y. Shi, and S. Walker, Protein Sci. 9:1045-1052, 2000) contains a hydrophobic patch surrounded by basic residues that may represent a membrane associatio...

journal_title：Journal of bacteriology

authors： van den Brink-van der Laan E,Boots JW,Spelbrink RE,Kool GM,Breukink E,Killian JA,de Kruijff B

更新日期：2003-07-01 00:00:00

abstract：:The relationship between the levels of cyclic nucleotides and dimorphic transition in Candida albicans was examined. The results showed that cells of this pathogenic fungus contained both cyclic adenosine 3',5'-monophosphate (cAMP) and cyclic guanosine 3',5'-monophosphate (cGMP), the concentration of the latter being ...

journal_title：Journal of bacteriology

authors： Niimi M,Niimi K,Tokunaga J,Nakayama H

更新日期：1980-06-01 00:00:00

abstract：:Streptococcus pneumoniae is the causative agent of multiple diseases, including otitis media, pneumonia, bacteremia, and meningitis. Pneumolysin (Ply), a member of the cholesterol-dependent cytolytic pore-forming toxins, is produced by virtually all clinical isolates of S. pneumoniae, and strains in which the Ply gene...

journal_title：Journal of bacteriology

authors： Price KE,Camilli A

更新日期：2009-04-01 00:00:00

abstract：:Rubrerythrin was purified by multistep chromatography under anaerobic, reducing conditions from the hyperthermophilic archaeon Pyrococcus furiosus. It is a homodimer with a molecular mass of 39.2 kDa and contains 2.9 +/- 0.2 iron atoms per subunit. The purified protein had peroxidase activity at 85 degrees C using hyd...

journal_title：Journal of bacteriology

authors： Weinberg MV,Jenney FE Jr,Cui X,Adams MW

更新日期：2004-12-01 00:00:00

abstract：:Conditions have been established for the preparation of spheroplasts of Saccharomyces cerevisiae which are able to increase their net content of protein, ribonucleic acid (RNA), and deoxyribonucleic acid (DNA), several-fold upon incubation in a medium stabilized with 1 m sorbitol. The rate of RNA and protein synthesis...

journal_title：Journal of bacteriology

authors： Hutchison HT,Hartwell LH

更新日期：1967-11-01 00:00:00

abstract：:SigF is an alternative sigma factor that is highly conserved among species of the genus Mycobacterium. In this study we identified the SigF regulon in Mycobacterium smegmatis using whole-genome microarray and promoter consensus analyses. In total, 64 genes in exponential phase and 124 genes in stationary phase are Sig...

journal_title：Journal of bacteriology

authors： Hümpel A,Gebhard S,Cook GM,Berney M

更新日期：2010-05-01 00:00:00

abstract：UNLABELLED:All methanofuran structural variants contain a basic core structure of 4-[N-(γ-l-glutamyl)-p-(β-aminoethyl)phenoxymethyl]-(aminomethyl)furan (APMF-Glu) but have different side chains depending on the source organism. Recently, we identified four genes (MfnA, MfnB, MfnC, and MfnD) that are responsible for the...

journal_title：Journal of bacteriology

authors： Wang Y,Xu H,Jones MK,White RH

更新日期：2015-09-01 00:00:00

abstract：:We examined tryptophan regulation in merodiploid hybrids in which a plasmid carrying the trp operon of Escherichia was introduced into Trp mutants of other enteric genera, or in which a plasmid carrying the trpR+ (repressor) gene of E. coli was transfered into fully constitutive trpR mutants of other genera. In these ...

journal_title：Journal of bacteriology

authors： Manson MD,Yanofsky C

更新日期：1976-05-01 00:00:00

abstract：:The tar locus of Escherichia coli specifies one of the major species of methyl-accepting proteins involved in the chemotactic behavior of this organism. The physical and genetic organization of the tar region was investigated with a series of specialized lambda transducing phages and plasmid clones. The tar gene was m...

journal_title：Journal of bacteriology

authors： Slocum MK,Parkinson JS

更新日期：1983-08-01 00:00:00

abstract：:Penicillin-binding proteins (PBPs), although characterized from several organisms, have so far not been studied in mycobacteria. The present study is the first characterization of a PBP from Mycobacterium smegmatis. The PBP was purified by solubilization of the membranes with Triton X-100 and successive chromatography...

journal_title：Journal of bacteriology

authors： Basu J,Chattopadhyay R,Kundu M,Chakrabarti P

更新日期：1992-07-01 00:00:00

abstract：:Bacterial cell division is mediated by a set of proteins that assemble to form a large multiprotein complex called the divisome. Recent studies in Bacillus subtilis and Escherichia coli indicate that cell division proteins are involved in multiple cooperative binding interactions, thus presenting a technical challenge...

journal_title：Journal of bacteriology

authors： Robichon C,King GF,Goehring NW,Beckwith J

更新日期：2008-09-01 00:00:00

abstract：:The Bacillus subtilis PurR mediates adenine repression and guanosine induction of purA. PRPP inhibits binding of PurR to DNA in vitro. Mutations in the PRPP binding motif of PurR caused strong repression regardless of purine exclusions or additions, establishing the role of PRPP as regulator of PurR. ...

journal_title：Journal of bacteriology

authors： Rappu P,Pullinen T,Mäntsälä P

更新日期：2003-11-01 00:00:00

abstract：:The enterohepatic Helicobacter species Helicobacter hepaticus colonizes the murine intestinal and hepatobiliary tract and is associated with chronic intestinal inflammation, gall stone formation, hepatitis, and hepatocellular carcinoma. Thus far, the role of H. hepaticus motility and flagella in intestinal colonizatio...

journal_title：Journal of bacteriology

authors： Sterzenbach T,Bartonickova L,Behrens W,Brenneke B,Schulze J,Kops F,Chin EY,Katzowitsch E,Schauer DB,Fox JG,Suerbaum S,Josenhans C

更新日期：2008-10-01 00:00:00

abstract：:The Escherichia coli purine repressor, PurR, binds to a 16-bp operator sequence and coregulates the genes for de novo synthesis of purine and pyrimidine nucleotides, formation of a one-carbon unit for biosynthesis, and deamination of cytosine. We have characterized the purified repressor. Chemical cross-linking indica...

journal_title：Journal of bacteriology

authors： Choi KY,Zalkin H

更新日期：1992-10-01 00:00:00

abstract：:The 5,10-methylenetetrahydrofolate dehydrogenase of heterotrophically grown Peptostreptococcus productus Marburg was purified to apparent homogeneity. The purified enzyme catalyzed the reversible oxidation of methylenetetrahydrofolate with NADP+ as the electron acceptor at a specific activity of 627 U/mg of protein. T...

journal_title：Journal of bacteriology

authors： Wohlfarth G,Geerligs G,Diekert G

更新日期：1991-02-01 00:00:00

abstract：:Bacillus subtilis possesses different enzymes for the utilization of plant cell wall polysaccharides. This includes a gene cluster containing galactan degradation genes (ganA and ganB), two transporter component genes (ganQ and ganP), and the sugar-binding lipoprotein-encoding gene ganS (previously known as cycB). The...

journal_title：Journal of bacteriology

authors： Watzlawick H,Morabbi Heravi K,Altenbuchner J

更新日期：2016-09-22 00:00:00

abstract：:The mecA-27r gene, which encodes PBP2a-27r, was modified by site-specific mutagenesis, resulting in replacement of the N-terminal membrane anchor with a short chelating peptide (CP-PBP2a-27r). CP-PBP2a-27r retained the same binding affinity for beta-lactam antibiotics as the wild-type enzyme. Approximately 95% pure CP...

journal_title：Journal of bacteriology

authors： Wu CY,Blaszczak LC,Smith MC,Skatrud PL

更新日期：1994-03-01 00:00:00

abstract：:A-factor (2-isocapryloyl-3R-hydroxymethyl-gamma-butyrolactone) is essential for the initiation of aerial mycelium formation in Streptomyces griseus. amfR is one of the genes which, when cloned on a low-copy-number plasmid, suppresses the aerial mycelium-negative phenotype of an A-factor-deficient mutant of S. griseus....

journal_title：Journal of bacteriology

authors： Ueda K,Hsheh CW,Tosaki T,Shinkawa H,Beppu T,Horinouchi S

更新日期：1998-10-01 00:00:00

abstract：:A macrorestriction map representing the complete physical map of the Rhodobacter sphaeroides 2.4.1 chromosomes has been constructed by ordering the chromosomal DNA fragments from total genomic DNA digested with the restriction endonucleases AseI, SpeI, DraI, and SnaBI. Junction fragments and multiple restriction endon...

journal_title：Journal of bacteriology

authors： Suwanto A,Kaplan S

更新日期：1989-11-01 00:00:00

abstract：:A gene for the constitutive ribosephosphate isomerase (rpiA) is highly cotransducible with serA at 56.2 min on the genetic linkage map of Escherichia coli K-12. Suppression of ribosephosphate isomerase A-negative mutants can occur by a regulator gene mutation permitting constitutive synthesis of the normally inducible...

journal_title：Journal of bacteriology

authors： Skinner AJ,Cooper RA

更新日期：1974-06-01 00:00:00

abstract：:Strain 768-28 was isolated from a hot spring in Kamchatka, Russia, and represents a novel member of the Vulcanisaeta genus. The complete genome sequence of this thermoacidophilic anaerobic crenarchaeon reveals genes for protein and carbohydrate-active enzymes, the Embden-Meyerhof and Entner-Doudoroff pathways for gluc...

journal_title：Journal of bacteriology

authors： Gumerov VM,Mardanov AV,Beletsky AV,Prokofeva MI,Bonch-Osmolovskaya EA,Ravin NV,Skryabin KG

更新日期：2011-05-01 00:00:00

abstract：:Porin F is one of the major proteins of the outer membrane of Pseudomonas aeruginosa. It forms water-filled pores of variable size. Porin F is a candidate for a vaccine against P. aeruginosa because it antigenically cross-reacts in all serotype strains of the International Antigenic Typing Scheme. We have isolated the...

journal_title：Journal of bacteriology

authors： Duchêne M,Schweizer A,Lottspeich F,Krauss G,Marget M,Vogel K,von Specht BU,Domdey H

更新日期：1988-01-01 00:00:00

abstract：:The force required to rupture bonds between individual Staphylococcus aureus MSCRAMMs and surfaces coated with extracellular matrix molecules has been quantified by using optical tweezers. The observed binding forces between fibrinogen or fibronectin and S. aureus MSCRAMMs occurred as an approximate integer multiple o...

journal_title：Journal of bacteriology

authors： Simpson KH,Bowden G,Höök M,Anvari B

更新日期：2003-03-01 00:00:00

abstract：:Although Salmonella typhimurium prefers neutral-pH environments, it can adapt to survive conditions of severe low-pH stress (pH 3.3). The process, termed the acid tolerance response (ATR), includes two distinct stages. The first stage, called pre-acid shock, is induced at pH 5.8 and involves the production of an induc...

journal_title：Journal of bacteriology

authors： Foster JW

更新日期：1993-04-01 00:00:00

abstract：:High-pressure liquid chromatography of a muramidase digest of murein sacculi from Caulobacter crescentus showed that the absence of D-alanine carboxypeptidase activity in the cells was reflected by a very high content of pentapeptide in the murein. Approximately half of the pentapeptide side chains were shown to conta...

journal_title：Journal of bacteriology

authors： Markiewicz Z,Glauner B,Schwarz U

更新日期：1983-11-01 00:00:00