Integration of rotation and piston motions in coiled-coil signal transduction.

Abstract:

:A coordinated response to a complex and dynamic environment requires an organism to simultaneously monitor and interpret multiple signaling cues. In bacteria and some eukaryotes, environmental responses depend on the histidine autokinases (HKs). For example, VirA, a large integral membrane HK from Agrobacterium tumefaciens, regulates the expression of virulence genes in response to signals from multiple molecular classes (phenol, pH, and sugar). The ability of this pathogen to perceive inputs from different known host signals within a single protein receptor provides an opportunity to understand the mechanisms of signal integration. Here we exploited the conserved domain organization of the HKs and engineered chimeric kinases to explore the signaling mechanisms of phenol sensing and pH/sugar integration. Our data implicate a piston-assisted rotation of coiled coils for integration of multiple inputs and regulation of critical responses during pathogenesis.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Gao R,Lynn DG

doi

10.1128/JB.00459-07

subject

Has Abstract

pub_date

2007-08-01 00:00:00

pages

6048-56

issue

16

eissn

0021-9193

issn

1098-5530

pii

JB.00459-07

journal_volume

189

pub_type

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