Proteomic characterization of Yersinia pestis virulence.

abstract：:A late-log-phase culture of an Escherichia coli nadB pncA double mutant took up 6-[7-14C]aminonicotinic acid and excreted 6-[14C]aminonicotinamide. This mutant also accumulated intracellularly several radioactive compounds which have been tentatively identified as 6-amino analogs of compounds in the pyridine nucleotid...

journal_title：Journal of bacteriology

authors： Cobb JR,Pearcy SC,Gholson RK

更新日期：1977-09-01 00:00:00

abstract：:The structural gene coding for the delta 5-3-ketosteroid isomerase (KSI) of Pseudomonas putida biotype B has been cloned, and its entire nucleotide sequence has been determined by a dideoxynucleotide chain termination method. A 2.1-kb DNA fragment containing the ksi gene was cloned from a P. putida biotype B genomic l...

journal_title：Journal of bacteriology

authors： Kim SW,Kim CY,Benisek WF,Choi KY

更新日期：1994-11-01 00:00:00

abstract：:The P(styA) promoter of Pseudomonas sp. strain Y2 controls expression of the styABCD genes, which are required for the conversion of styrene to phenylacetate, which is further catabolized by the products of two paa gene clusters. Two PaaX repressor proteins (PaaX1 and PaaX2) regulate transcription of the paa gene clus...

journal_title：Journal of bacteriology

authors： del Peso-Santos T,Bartolomé-Martín D,Fernández C,Alonso S,García JL,Díaz E,Shingler V,Perera J

更新日期：2006-07-01 00:00:00

abstract：:Antibody in hyperimmune rabbit antisera specific for Staphylococcus aureus teichoic acid was shown to be associated with the IgM fraction. Treatment of such sera with mercaptoethanol destroyed its activity in passive mouse protection tests, whereas absorption with antirabbit IgG had no effect. Antibody response in nor...

journal_title：Journal of bacteriology

authors： Yoshida K,Ekstedt RD

更新日期：1968-11-01 00:00:00

abstract：:Many bacterial species produce capsular polysaccharides that contribute to pathogenesis through evasion of the host innate immune system. The gram-positive pathogen Enterococcus faecalis was previously reported to produce one of four capsule serotypes (A, B, C, or D). Previous studies describing the four capsule serot...

journal_title：Journal of bacteriology

authors： Thurlow LR,Thomas VC,Hancock LE

更新日期：2009-10-01 00:00:00

abstract：:Protoplasts of Saccharomyces cerevisiae were mixed with linear DNA plasmids, pGKl1 and pGKl2, isolated from a Kluyveromyces lactis killer strain and treated with polyethylene glycol. Out of 2,000 colonies regenerated on a nonselective medium, two killer transformants were obtained. The pGKl plasmids and the killer cha...

journal_title：Journal of bacteriology

authors： Gunge N,Murata K,Sakaguchi K

更新日期：1982-07-01 00:00:00

abstract：:Loss of the ability of Pseudomonas syringae pv. "phaseolicola" NPS3121 to elicit a hypersensitive response on tobacco and other nonhost plants was associated with loss of pathogenicity on the susceptible host bean. Eight independent, prototrophic transposon Tn5 insertion mutants which had lost the ability to elicit a ...

journal_title：Journal of bacteriology

authors： Lindgren PB,Peet RC,Panopoulos NJ

更新日期：1986-11-01 00:00:00

abstract：UNLABELLED:Many bacteria utilize actin-like proteins to direct peptidoglycan (PG) synthesis. MreB and MreB-like proteins are thought to act as scaffolds, guiding the localization and activity of key PG-synthesizing proteins during cell elongation. Despite their critical role in viability and cell shape maintenance, ver...

journal_title：Journal of bacteriology

authors： Duan Y,Sperber AM,Herman JK

更新日期：2016-07-13 00:00:00

abstract：:Siderophore-mediated iron transport in Pseudomonas aeruginosa is dependent upon the cytoplasmic membrane-associated TonB1 energy coupling protein for activity. To assess the functional significance of the various regions of this molecule and to identify functionally important residues, the tonB1 gene was subjected to ...

journal_title：Journal of bacteriology

authors： Zhao Q,Poole K

更新日期：2002-03-01 00:00:00

abstract：:We provide experimental evidence for RNase III-dependent processing in helix 9 of the 23S rRNA as a general feature of many species in the alpha subclass of Proteobacteria (alpha-Proteobacteria). We investigated 12 Rhodobacter, Rhizobium, Sinorhizobium, Rhodopseudomonas, and Bartonella strains. The processed region is...

journal_title：Journal of bacteriology

authors： Evguenieva-Hackenberg E,Klug G

更新日期：2000-09-01 00:00:00

abstract：:The Rnf complex is a respiratory enzyme that catalyzes the oxidation of reduced ferredoxin to the reduction of NAD+, and the negative free energy change of this reaction is used to generate a transmembrane ion gradient. In one class of anaerobic acetogenic bacteria, the Rnf complex is believed to be essential for ener...

journal_title：Journal of bacteriology

authors： Westphal L,Wiechmann A,Baker J,Minton NP,Müller V

更新日期：2018-10-10 00:00:00

abstract：:Pseudomonas aeruginosa hemolytic phospholipase C (PlcH) degrades phosphatidylcholine (PC), an abundant lipid in cell membranes and lung surfactant. A ΔplcHR mutant, known to be defective in virulence in animal models, was less able to colonize epithelial cell monolayers and was defective in biofilm formation on plasti...

journal_title：Journal of bacteriology

authors： Jackson AA,Gross MJ,Daniels EF,Hampton TH,Hammond JH,Vallet-Gely I,Dove SL,Stanton BA,Hogan DA

更新日期：2013-07-01 00:00:00

abstract：:Agrobacterium tumefaciens wild-type strains have a unique quorum-sensing (QS)-dependent Ti plasmid conjugative transfer phenotype in which QS signaling is activated by corresponding conjugative opine inducers. Strain K588, with a nopaline-type chromosomal background harboring an octopine-type Ti plasmid, however, is a...

journal_title：Journal of bacteriology

authors： Wang C,Zhang HB,Chen G,Chen L,Zhang LH

更新日期：2006-04-01 00:00:00

abstract：:The biological activity of Odontomyces viscosus, which has been reported to cause periodontal disease in hamsters, was examined. The microorganism was cultured anaerobically in Brain Heart Infusion broth, and the cells were harvested. The washed cells were injected intradermally into the abdomen of rabbits. After 72 h...

journal_title：Journal of bacteriology

authors： Schuster GS,Hayashi JA,Bahn AN

更新日期：1967-01-01 00:00:00

abstract：:Six uvr mutants of Saccharomyces cerevisiae with hypersensitivity to ultraviolet (UV) light were isolated after mutagen treatment with ethylmethanesulfonate. UV sensitivity ranges from moderate to extreme, and four of the mutants are also sensitive to nitrous acid. Ranking in terms of UV sensitivity does not parallel ...

journal_title：Journal of bacteriology

authors： Snow R

更新日期：1967-09-01 00:00:00

abstract：:Raps, Shirley (University of Illinois, Urbana) and R. D. DeMoss. Glycolytic enzymes in Zymomonas mobilis. J. Bacteriol. 84:115-118. 1962-An enzyme extract of Zymomonas mobilis (Pseudomonas lindneri) was capable of fermenting glucose-6-phosphate to CO(2) and ethanol. The extract was found to contain phosphohexoisomeras...

journal_title：Journal of bacteriology

authors： RAPS S,DEMOSS RD

更新日期：1962-07-01 00:00:00

abstract：:2-Aminonumconic 6-semialdehyde is an unstable intermediate in the biodegradation of nitrobenzene and 2-aminophenol by Pseudomonas pseudoalcaligenes JS45. Previous work has shown that enzymes in cell extracts convert 2-aminophenol to 2-aminomuconate in the presence of NAD+. In the present work, 2-aminomuconic semialdeh...

journal_title：Journal of bacteriology

authors： He Z,Davis JK,Spain JC

更新日期：1998-09-01 00:00:00

abstract：:Growing cells of Campylobacter coli and C. jejuni were naturally transformed by naked DNA without the requirement for any special treatment. Transformation frequencies for homologous chromosomal DNA were approximately 10(-3) transformants per recipient cell in C. coli and 10(-4) in C. jejuni. Maximum competence was fo...

journal_title：Journal of bacteriology

authors： Wang Y,Taylor DE

更新日期：1990-02-01 00:00:00

abstract：:The Escherichia coli dnaN159 allele encodes a mutant form of the beta-sliding clamp (beta159) that is impaired for interaction with the replicative DNA polymerase (Pol), Pol III. In addition, strains bearing the dnaN159 allele require functional Pol I for viability. We have utilized a combination of genetic and bioche...

journal_title：Journal of bacteriology

authors： Maul RW,Sanders LH,Lim JB,Benitez R,Sutton MD

更新日期：2007-07-01 00:00:00

abstract：:The presence of the altered lysine transfer ribonucleic acid (tRNA) in Bacillus subtilis spores is strongly dependent on the medium on which the cells were sporulated. Cells sporulated on synthetic media or dilute complex media contain little or none of the new component, whereas those sporulated on concentrated compl...

journal_title：Journal of bacteriology

authors： Lazzarini RA,Santangelo E

更新日期：1967-07-01 00:00:00

abstract：:A 4.2-kilobase cryptic Bacteroides plasmid, pB8-51, is found in several colonic Bacteroides species. To determine whether pB8-51 is mobilized by any of the known Bacteroides conjugal elements, we constructed an Escherichia coli-Bacteroides shuttle vector, pVAL-1, which contains pB8-51. We constructed Bacteroides unifo...

journal_title：Journal of bacteriology

authors： Valentine PJ,Shoemaker NB,Salyers AA

更新日期：1988-03-01 00:00:00

abstract：:The flaW, flaU, and flaV genes of Salmonella typhimurium LT2 were cloned into pBR322. These genes were mapped on the cloned DNA fragments by restriction endonuclease analysis and construction of the deletion derivatives. Their gene products were identified, by the minicell method, as proteins whose molecular weights w...

journal_title：Journal of bacteriology

authors： Homma M,Kutsukake K,Iino T

更新日期：1985-08-01 00:00:00

abstract：:Das, S. K. (University of Calcutta, Calcutta, India) and G. C. Chatterjee. Pyrithiamine adaptation of Staphylococcus aureus. I. Adaptation and carbohydrate utilization. J. Bacteriol. 83:1251-1259. 1962.-Staphylococcus aureus has been adapted to pyrithiamine, a thiamine analogue; as a result of this adaptation, the col...

journal_title：Journal of bacteriology

authors： DAS SK,CHATTERJEE GC

更新日期：1962-06-01 00:00:00

abstract：:S-Adenosyl-L-methionine:precorrin-2 methyltransferase (SP2MT), which catalyzes the C-20 methylation of precorrin-2 to precorrin-3, was purified to homogeneity from extracts of a recombinant strain of Pseudomonas denitrificans derived from a cobalamin-overproducing strain. Ammonium sulfate fractionation followed by chr...

journal_title：Journal of bacteriology

authors： Thibaut D,Couder M,Crouzet J,Debussche L,Cameron B,Blanche F

更新日期：1990-11-01 00:00:00

abstract：:Entry into intestinal epithelial cells is an essential step in the pathogenesis of Salmonella infections. Our laboratory has previously identified a genetic locus, inv, that is necessary for efficient entry of Salmonella typhimurium into cultured epithelial cells. We have carried out a molecular and functional analysi...

journal_title：Journal of bacteriology

authors： Eichelberg K,Ginocchio CC,Galán JE

更新日期：1994-08-01 00:00:00

abstract：:We describe the further utilization of a genetic screen that identifies mutations defective in the assembly of proteins into the Escherichia coli cytoplasmic membrane. The screen yielded mutations in each of the known genes encoding components of the E. coli signal recognition particle pathway: ffh, ffs, and ftsY, whi...

journal_title：Journal of bacteriology

authors： Tian H,Beckwith J

更新日期：2002-01-01 00:00:00

abstract：:For Escherichia coli, growth on acetate requires the induction of the enzymes of the glyoxylate bypass, isocitrate lyase and malate synthase. The branch point between the glyoxylate bypass and the Krebs cycle is controlled by phosphorylation of isocitrate dehydrogenase (IDH), inhibiting that enzyme's activity and thus...

journal_title：Journal of bacteriology

authors： Ikeda T,LaPorte DC

更新日期：1991-03-01 00:00:00

abstract：:In Escherichia coli K-12, expression of the lysU gene is regulated by the lrp gene product, as indicated by an increase in the level of lysyl-tRNA synthetase activity and LysU protein in an lrp mutant. Comparison of the patterns of protein expression visualized by two-dimensional gel electrophoresis indicated that Lys...

journal_title：Journal of bacteriology

authors： Lin R,Ernsting B,Hirshfield IN,Matthews RG,Neidhardt FC,Clark RL,Newman EB

更新日期：1992-05-01 00:00:00

abstract：:In this study, we identified and characterized the enzymatic properties of MG_186, a calcium-dependent Mycoplasma genitalium nuclease. MG_186 displays the hallmarks of nucleases, as indicated by its amino acid sequence similarity to other nucleases. We cloned, UGA corrected, expressed, purified, and demonstrated that ...

journal_title：Journal of bacteriology

authors： Li L,Krishnan M,Baseman JB,Kannan TR

更新日期：2010-10-01 00:00:00

abstract：:We report that transformation of Streptomyces lividans with cloned DNA of the SLP1 genetic element results in integration of the element at the same chromosomal locus (attB) normally occupied by SLP1 in its original host, Streptomyces coelicolor, and in S. lividans that has received SLP1 by mating. We constructed SLP1...

journal_title：Journal of bacteriology

authors： Omer CA,Stein D,Cohen SN

更新日期：1988-05-01 00:00:00