Decreased chromate uptake in Pseudomonas fluorescens carrying a chromate resistance plasmid.

Abstract:

:CrO4(2-) resistance in Pseudomonas fluorescens LB300(pLHB1) was related to reduced uptake of CrO4(2-) relative to the plasmidless strain LB303. 51CrO4(2-) was transported mainly via the SO4(2-) active transport system; thus, cells grown with 0.15 mM cysteine, a repressor of the SO4(2-) transport system, were much more resistant to CrO4(2-) than those grown with 0.15 mM djenkolic acid, which derepressed the 35SrO4(2-) uptake system. Kinetics of 51CrO4(2-) uptake by P. fluorescens with and without the plasmid showed that the Vmax for 51CrO4(2-) uptake with the resistant strain was 2.2 times less than the Vmax for the sensitive strain, whereas the Km remained constant.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Ohtake H,Cervantes C,Silver S

doi

10.1128/jb.169.8.3853-3856.1987

subject

Has Abstract

pub_date

1987-08-01 00:00:00

pages

3853-6

issue

8

eissn

0021-9193

issn

1098-5530

journal_volume

169

pub_type

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