Transcriptomic analysis of the sulfate starvation response of Pseudomonas aeruginosa.

Abstract:

:Pseudomonas aeruginosa is an opportunistic pathogen that causes a number of infections in humans, but is best known for its association with cystic fibrosis. It is able to use a wide range of sulfur compounds as sources of sulfur for growth. Gene expression in response to changes in sulfur supply was studied in P. aeruginosa E601, a cystic fibrosis isolate that displays mucin sulfatase activity, and in P. aeruginosa PAO1. A large family of genes was found to be upregulated by sulfate limitation in both isolates, encoding sulfatases and sulfonatases, transport systems, oxidative stress proteins, and a sulfate-regulated TonB/ExbBD complex. These genes were localized in five distinct islands on the genome and encoded proteins with a significantly reduced content of cysteine and methionine. Growth of P. aeruginosa E601 with mucin as the sulfur source led not only to a sulfate starvation response but also to induction of genes involved with type III secretion systems.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Tralau T,Vuilleumier S,Thibault C,Campbell BJ,Hart CA,Kertesz MA

doi

10.1128/JB.00889-07

subject

Has Abstract

pub_date

2007-10-01 00:00:00

pages

6743-50

issue

19

eissn

0021-9193

issn

1098-5530

pii

JB.00889-07

journal_volume

189

pub_type

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