Effect of some proteins on the yeast cell membrane.

Abstract:

:Yeast cells, Candida utilis, in water suspension and in the absence of electrolytes were found to be very sensitive to several proteins of moderate size, including ribonuclease, protamine, lysozyme, bovine serum albumin, cytochrome c, and myoglobin. Viability ceases rapidly, and ultraviolet-absorbing compounds (260 mmu) and the amino acid pool are released into the medium. The ultraviolet-absorbing material appears to be the nucleotide and coenzyme fraction usually extracted by 0.2 n perchloric acid at low temperature. The ribonucleic acid fraction remains in the cell ghosts and can be released by ribonuclease. The enzymatic properties of some of these proteins have no relation to their damaging effect on the cell membrane. Poly-l-lysine shows the same activity.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Yphantis DA,Dainko JL,Schlenk F

doi

10.1128/JB.94.5.1509-1515.1967

subject

Has Abstract

pub_date

1967-11-01 00:00:00

pages

1509-15

issue

5

eissn

0021-9193

issn

1098-5530

journal_volume

94

pub_type

杂志文章
  • Yield coefficients of Thiobacillus neapolitanus in continuous culture.

    abstract::Thiobacillus neapolitanus, when grown in continuous culture with thiosulfate limiting growth, possessed an apparent maximal molar growth yield of 8.0 g (dry weight) per mole of thiosulfate. The substrate requirement for energy of maintenance was the highest yet reported, amounting to 21.8 mmoles of thiosulfate per g p...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.93.3.874-878.1967

    authors: Hempfling WP,Vishniac W

    更新日期:1967-03-01 00:00:00

  • Transport of diamines by Enterococcus faecalis is mediated by an agmatine-putrescine antiporter.

    abstract::Enterococcus faecalis ATCC 11700 is able to use arginine and the diamine agmatine as a sole energy source. Via the highly homologous deiminase pathways, arginine and agmatine are converted into CO2, NH3, and the end products ornithine and putrescine, respectively. In the arginine deiminase pathway, uptake of arginine ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.10.4522-4527.1988

    authors: Driessen AJ,Smid EJ,Konings WN

    更新日期:1988-10-01 00:00:00

  • Purification and properties of a hydrogenase from Desulfovibrio vulgaris.

    abstract::The soluble hydrogenase of Desulfovibrio vulgaris was purified and some of its properties are described. The molecular weight was determined for the enzyme by sedimentation equilibrium (45,400) and amino acid analysis (44,800). The hydrogenase appears to be a loosely coiled molecule or to have a high axial ratio, whic...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.105.1.249-258.1971

    authors: Haschke RH,Campbell LL

    更新日期:1971-01-01 00:00:00

  • Genetic mapping of katA, a locus that affects catalase 1 level in Bacillus subtilis.

    abstract::Several mutants of Bacillus subtilis deficient in catalase synthesis generated by nitrosoguanidine mutagenesis have been used to map a locus affecting catalase activity. Two- and three-factor bacteriophage PBS1 transductional crosses were used to locate the locus, named katA, between recH and thiA with 98% linkage to ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.12.5848-5851.1987

    authors: Loewen PC,Switala J

    更新日期:1987-12-01 00:00:00

  • Sulfur amino acid metabolism and its control in Lactococcus lactis IL1403.

    abstract::Cysteine and methionine availability influences many processes in the cell. In bacteria, transcription of the specific genes involved in the synthesis of these two amino acids is usually regulated by different mechanisms or regulators. Pathways for the synthesis of cysteine and methionine and their interconversion wer...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.11.3762-3778.2005

    authors: Sperandio B,Polard P,Ehrlich DS,Renault P,Guédon E

    更新日期:2005-06-01 00:00:00

  • Respiratory-competent conditional developmental mutant of Mucor racemosus.

    abstract::A conditional developmental mutant of Mucor racemosus which is capable of oxidative energy metabolism is described. Unlike the wild-type strain the mutant was highly fermentative and exhibited the yeast morphology when grown aerobically in glucose-containing media. The high fermentative activity and yeast morphology u...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.164.3.1049-1056.1985

    authors: Borgia PT,Gokul NK,Phillips GJ

    更新日期:1985-12-01 00:00:00

  • Genomic analysis reveals chromosomal variation in natural populations of the uncultured psychrophilic archaeon Cenarchaeum symbiosum.

    abstract::Molecular phylogenetic surveys have recently revealed an ecologically widespread crenarchaeal group that inhabits cold and temperate terrestrial and marine environments. To date these organisms have resisted isolation in pure culture, and so their phenotypic and genotypic characteristics remain largely unknown. To cha...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.19.5003-5009.1998

    authors: Schleper C,DeLong EF,Preston CM,Feldman RA,Wu KY,Swanson RV

    更新日期:1998-10-01 00:00:00

  • Isolation and analysis of the Xanthomonas alkyl hydroperoxide reductase gene and the peroxide sensor regulator genes ahpC and ahpF-oxyR-orfX.

    abstract::From Xanthomonas campestris pv. phaseoli, we have isolated by two independent methods genes involved in peroxide detoxification (ahpC and ahpF), a gene involved in peroxide sensing and transcription regulation (oxyR), and a gene of unknown function (orfX). Amino acid sequence analysis of AhpC, AhpF, and OxyR showed hi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.12.3944-3949.1997

    authors: Loprasert S,Atichartpongkun S,Whangsuk W,Mongkolsuk S

    更新日期:1997-06-01 00:00:00

  • Effect of an oxygen-tolerant bifurcating butyryl coenzyme A dehydrogenase/electron-transferring flavoprotein complex from Clostridium difficile on butyrate production in Escherichia coli.

    abstract::The butyrogenic genes from Clostridium difficile DSM 1296(T) have been cloned and expressed in Escherichia coli. The enzymes acetyl-coenzyme A (CoA) C-acetyltransferase, 3-hydroxybutyryl-CoA dehydrogenase, crotonase, phosphate butyryltransferase, and butyrate kinase and the butyryl-CoA dehydrogenase complex composed o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00321-13

    authors: Aboulnaga el-H,Pinkenburg O,Schiffels J,El-Refai A,Buckel W,Selmer T

    更新日期:2013-08-01 00:00:00

  • Mechanics of bacterial macrofiber initiation.

    abstract::The twisting and writhing during growth of single-cell filaments of Bacillus subtilis which lead to macrofiber formation was studied in both left- and right-handed forms of strains FJ7 and RHX. Filament bending, touching, and loop formation (folding), followed by winding up into a double-strand fiber, were documented....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.24.7060-7069.1995

    authors: Mendelson NH,Thwaites JJ,Kessler JO,Li C

    更新日期:1995-12-01 00:00:00

  • Reconstitution of an active lactose carrier in vivo by simultaneous synthesis of two complementary protein fragments.

    abstract::Escherichia coli lactose permease mediates the proton-driven translocation of galactosides across the cytoplasmic membrane. To define regions important for membrane insertion as well as for biological function, we constructed plasmids encoding different portions of the lactose carrier. Among several lacY deletions, tw...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.9.5374-5381.1990

    authors: Wrubel W,Stochaj U,Sonnewald U,Theres C,Ehring R

    更新日期:1990-09-01 00:00:00

  • The beta-oxidation systems of Escherichia coli and Salmonella enterica are not functionally equivalent.

    abstract::Based on its genome sequence, the pathway of beta-oxidative fatty acid degradation in Salmonella enterica serovar Typhimurium LT2 has been thought to be identical to the well-characterized Escherichia coli K-12 system. We report that wild-type strains of S. enterica grow on decanoic acid, whereas wild-type E. coli str...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.188.2.599-608.2006

    authors: Iram SH,Cronan JE

    更新日期:2006-01-01 00:00:00

  • In vivo and in vitro kinetics of nitrogenase.

    abstract::We measured some of the kinetic parameters of nitrogenase to intact systems of Clostridium pasteurianum and Klebsiella pneumoniae to compare them with the kinetics of the enzyme in vitro. We found that the enzyme showed multiple apparent Km values for acetylene reduction in vivo, as it does in vitro. Carbon monoxide w...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.141.3.1230-1238.1980

    authors: Davis LC,Wang YL

    更新日期:1980-03-01 00:00:00

  • Possible role of lectins in mycoparasitism.

    abstract::Lectin activity in a host-mycoparasite relationship was demonstrated with Rhizoctonia solani and Trichoderma harzianum. Attachment of O but not A and B erythrocytes to hyphae occurred on R. solani but not on its mycoparasite. This phenomenon, which was Ca2+ and Mn2+ dependent, was prevented by galactose, present in T....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.154.3.1431-1435.1983

    authors: Elad Y,Barak R,Chet I

    更新日期:1983-06-01 00:00:00

  • EFFECT OF HIGH FAT DIETS ON INTESTINAL MICROFLORA AND SERUM CHOLESTEROL IN RATS.

    abstract::Graber, C. D. (Baylor University College of Medicine, Houston, Tex.), R. M. O'Neal, and E. R. Rabin. Effect of high fat diets on intestinal microflora and serum cholesterol in rats. J. Bacteriol. 89:47-51. 1965.-Differential bacterial counts of feces and total plasma cholesterol determinations were performed on 60 Wis...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.89.1.47-51.1965

    authors: GRABER CD,O'NEAL RM,RABIN ER

    更新日期:1965-01-01 00:00:00

  • Periplasmic structure of frozen-etched and negatively stained cells of Bacillus licheniformis as correlated with penicillinase formation.

    abstract::Bacillus licheniformis strain 749/C (constitutive for penicillinase formation) and uninduced cells of strain 749 (penicillinase-inducible) were examined after freezeetching. In the early stationary phase, strain 749/C organisms had clusters of vesicles (30 to 40 nm in diameter) on the outer surface of the plasma membr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.100.2.1002-1009.1969

    authors: Ghosh BK,Lampen JO,Remsen CC

    更新日期:1969-11-01 00:00:00

  • Molecular characterization of the hemolysin determinant of Serratia marcescens.

    abstract::The nucleotide sequence of a 7.3-kilobase-pair fragment of DNA encoding a hemolytic activity from Serratia marcescens was determined. Two large open reading frames were identified, designated shlA (Serratia hemolysin) and shlB, capable of encoding polypeptides of 165, 056 and 61,897 molecular weight, respectively. Bot...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.7.3177-3188.1988

    authors: Poole K,Schiebel E,Braun V

    更新日期:1988-07-01 00:00:00

  • Circular and linear plasmids of Lyme disease spirochetes have extensive homology: characterization of a repeated DNA element.

    abstract::We have cloned three copies of a repeated DNA segment from Borrelia burgdorferi sensu stricto strain B31, present on both circular and linear plasmids of this and other B. burgdorferi sensu lato strains. The DNA sequences are characterized by a highly homologous segment containing two open reading frames (ORFs), ORF-A...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.8.2287-2298.1996

    authors: Zückert WR,Meyer J

    更新日期:1996-04-01 00:00:00

  • Bacillus subtilis gene cluster involved in calcium carbonate biomineralization.

    abstract::Calcium carbonate precipitation, a widespread phenomenon among bacteria, has been investigated due to its wide range of scientific and technological implications. Nevertheless, little is known of the molecular mechanisms by which bacteria foster calcium carbonate mineralization. In our laboratory, we are studying calc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01450-06

    authors: Barabesi C,Galizzi A,Mastromei G,Rossi M,Tamburini E,Perito B

    更新日期:2007-01-01 00:00:00

  • Regulation of Aspergillus nidulans penicillin biosynthesis and penicillin biosynthesis genes acvA and ipnA by glucose.

    abstract::Expression of the Aspergillus nidulans penicillin biosynthesis genes acvA and ipnA, encoding delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase and isopenicillin N synthetase, respectively, was analyzed. The intergenic region carrying the divergently oriented promoters was fused in frame in both orientations ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.11.3789-3799.1992

    authors: Brakhage AA,Browne P,Turner G

    更新日期:1992-06-01 00:00:00

  • Distance between alleles as a determinant of linkage in natural transformation of Acinetobacter calcoaceticus.

    abstract::Cotransformation frequencies of 16, 39, 51, and 60% were observed when donor alleles were separated by distances of 9.2, 7.4, 6.3, and 5.1 kb, respectively, in donor Acinetobacter calcoaceticus DNA. A different and unexpected pattern was observed when the distance between recipient alleles was reduced from 9.2 to 5.1 ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.20.6015-6017.1995

    authors: Kloos DU,DiMarco AA,Elsemore DA,Timmis KN,Ornston LN

    更新日期:1995-10-01 00:00:00

  • Kinetic analysis of Clostridium cellulolyticum carbohydrate metabolism: importance of glucose 1-phosphate and glucose 6-phosphate branch points for distribution of carbon fluxes inside and outside cells as revealed by steady-state continuous culture.

    abstract::During the growth of Clostridium cellulolyticum in chemostat cultures with ammonia as the growth-limiting nutrient, as much as 30% of the original cellobiose consumed by C. cellulolyticum was converted to cellotriose, glycogen, and polysaccharides regardless of the specific growth rates. Whereas the specific consumpti...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.7.2010-2017.2000

    authors: Guedon E,Desvaux M,Petitdemange H

    更新日期:2000-04-01 00:00:00

  • Nucleotide sequence of the Rhodobacter capsulatus fruK gene, which encodes fructose-1-phosphate kinase: evidence for a kinase superfamily including both phosphofructokinases of Escherichia coli.

    abstract::The fruK gene encoding fructose-1-phosphate kinase (FruK), located within the fructose (fru)-catabolic operon of Rhodobacter capsulatus, was sequenced. FruK of R. capsulatus (316 amino acids; molecular weight = 31,232) is the same size as and is homologous to FruK of Escherichia coli, phosphofructokinase B (PfkB) of E...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.10.3117-3127.1991

    authors: Wu LF,Reizer A,Reizer J,Cai B,Tomich JM,Saier MH Jr

    更新日期:1991-05-01 00:00:00

  • Gap-filling repair synthesis induced by ultraviolet light in a Bacillus subtilis Uvr- mutant.

    abstract::Deoxyribonucleic acid repair synthesis was studied in one wild-type and two mutant strains of Bacillus subtilis that are defective in excision of pyrimidine dimers. The cells were irradiated with ultraviolet light, and 6-(p-hydroxyphenyl-azo)-uracil was used to block replicative synthesis, allowing only repair synthes...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.139.1.239-246.1979

    authors: Hadden CT

    更新日期:1979-07-01 00:00:00

  • Transcriptional regulation of delta-aminolevulinic acid dehydratase synthesis by oxygen in Bradyrhizobium japonicum and evidence for developmental control of the hemB gene.

    abstract::An increased demand for cytochromes is associated with symbiotic development and microaerobic metabolism in the bacterium Bradyrhizobium japonicum, and evidence suggests that hemB, rather than hemA, is the first essential bacterial heme synthesis gene in symbiosis with soybean. Steady-state levels of mRNA and protein ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.11.3706-3710.1997

    authors: Chauhan S,O'Brian MR

    更新日期:1997-06-01 00:00:00

  • Expression of the recA gene of Pseudomonas aeruginosa PAO is inducible by DNA-damaging agents.

    abstract::Western (immunoblot) analysis using Escherichia coli anti-RecA antiserum revealed that expression of the RecA protein of Pseudomonas aeruginosa PAO is induced upon exposure of the bacterium to UV irradiation or norfloxacin, a quinolone related to nalidixic acid. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.5.2385-2387.1988

    authors: Miller RV,Kokjohn TA

    更新日期:1988-05-01 00:00:00

  • Hexuronic acid dehydrogenase of Agrobacterium tumefaciens.

    abstract::Growth of Agrobacterium tumefaciens on d-glucuronic acid (GlcUA) or d-galacturonic acid (GalUA) induces formation of hexuronic acid dehydrogenase [d-aldohexuronic acid: nicotinamide adenine dinucleotide (NAD) oxidoreductase]. The dehydrogenase, which irreversibly converts GlcUA or GalUA to the corresponding hexaric ac...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.99.3.667-673.1969

    authors: Chang YF,Feingold DS

    更新日期:1969-09-01 00:00:00

  • GENETIC INSTABILITY IN ESCHERICHIA COLI.

    abstract::Schwartz, Norman M. (Yale University, New Haven, Conn.). Genetic instability in Escherichia coli. J. Bacteriol. 89:712-717. 1965.-Results obtained from a transductional analysis demonstrated that an extremely unstable reversion of lac(-) is a suppressor mutation. This mutation suppresses 2 of 22 different lac(-) allel...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.89.3.712-717.1965

    authors: SCHWARTZ NM

    更新日期:1965-03-01 00:00:00

  • Plasmid DNA from the acetotrophic methanogen Methanosarcina acetivorans.

    abstract::Nine acetotrophic and three methylotrophic strains of methane-producing bacteria were screened for the presence of plasmid DNA. Plasmids were detected in three marine isolates, including Methanosarcina acetivorans. All three plasmids appeared to be similar based on size and restriction site analyses. The plasmid from ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.10.4979-4982.1988

    authors: Sowers KR,Gunsalus RP

    更新日期:1988-10-01 00:00:00

  • Characterization of mutations in the metY-nusA-infB operon that suppress the slow growth of a DeltarimM mutant.

    abstract::The RimM protein in Escherichia coli is associated with free 30S ribosomal subunits but not with 70S ribosomes. A DeltarimM mutant shows a sevenfold-reduced growth rate and a reduced translational efficiency, probably as a result of aberrant assembly of the ribosomal 30S subunits. The slow growth and translational def...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.20.6095-6106.2001

    authors: Bylund GO,Lövgren JM,Wikström PM

    更新日期:2001-10-01 00:00:00