Abstract:
:Yeast cells, Candida utilis, in water suspension and in the absence of electrolytes were found to be very sensitive to several proteins of moderate size, including ribonuclease, protamine, lysozyme, bovine serum albumin, cytochrome c, and myoglobin. Viability ceases rapidly, and ultraviolet-absorbing compounds (260 mmu) and the amino acid pool are released into the medium. The ultraviolet-absorbing material appears to be the nucleotide and coenzyme fraction usually extracted by 0.2 n perchloric acid at low temperature. The ribonucleic acid fraction remains in the cell ghosts and can be released by ribonuclease. The enzymatic properties of some of these proteins have no relation to their damaging effect on the cell membrane. Poly-l-lysine shows the same activity.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Yphantis DA,Dainko JL,Schlenk Fdoi
10.1128/JB.94.5.1509-1515.1967subject
Has Abstractpub_date
1967-11-01 00:00:00pages
1509-15issue
5eissn
0021-9193issn
1098-5530journal_volume
94pub_type
杂志文章abstract::Thiobacillus neapolitanus, when grown in continuous culture with thiosulfate limiting growth, possessed an apparent maximal molar growth yield of 8.0 g (dry weight) per mole of thiosulfate. The substrate requirement for energy of maintenance was the highest yet reported, amounting to 21.8 mmoles of thiosulfate per g p...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.93.3.874-878.1967
更新日期:1967-03-01 00:00:00
abstract::Enterococcus faecalis ATCC 11700 is able to use arginine and the diamine agmatine as a sole energy source. Via the highly homologous deiminase pathways, arginine and agmatine are converted into CO2, NH3, and the end products ornithine and putrescine, respectively. In the arginine deiminase pathway, uptake of arginine ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.10.4522-4527.1988
更新日期:1988-10-01 00:00:00
abstract::The soluble hydrogenase of Desulfovibrio vulgaris was purified and some of its properties are described. The molecular weight was determined for the enzyme by sedimentation equilibrium (45,400) and amino acid analysis (44,800). The hydrogenase appears to be a loosely coiled molecule or to have a high axial ratio, whic...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.105.1.249-258.1971
更新日期:1971-01-01 00:00:00
abstract::Several mutants of Bacillus subtilis deficient in catalase synthesis generated by nitrosoguanidine mutagenesis have been used to map a locus affecting catalase activity. Two- and three-factor bacteriophage PBS1 transductional crosses were used to locate the locus, named katA, between recH and thiA with 98% linkage to ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.12.5848-5851.1987
更新日期:1987-12-01 00:00:00
abstract::Cysteine and methionine availability influences many processes in the cell. In bacteria, transcription of the specific genes involved in the synthesis of these two amino acids is usually regulated by different mechanisms or regulators. Pathways for the synthesis of cysteine and methionine and their interconversion wer...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.11.3762-3778.2005
更新日期:2005-06-01 00:00:00
abstract::A conditional developmental mutant of Mucor racemosus which is capable of oxidative energy metabolism is described. Unlike the wild-type strain the mutant was highly fermentative and exhibited the yeast morphology when grown aerobically in glucose-containing media. The high fermentative activity and yeast morphology u...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.164.3.1049-1056.1985
更新日期:1985-12-01 00:00:00
abstract::Molecular phylogenetic surveys have recently revealed an ecologically widespread crenarchaeal group that inhabits cold and temperate terrestrial and marine environments. To date these organisms have resisted isolation in pure culture, and so their phenotypic and genotypic characteristics remain largely unknown. To cha...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.19.5003-5009.1998
更新日期:1998-10-01 00:00:00
abstract::From Xanthomonas campestris pv. phaseoli, we have isolated by two independent methods genes involved in peroxide detoxification (ahpC and ahpF), a gene involved in peroxide sensing and transcription regulation (oxyR), and a gene of unknown function (orfX). Amino acid sequence analysis of AhpC, AhpF, and OxyR showed hi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.12.3944-3949.1997
更新日期:1997-06-01 00:00:00
abstract::The butyrogenic genes from Clostridium difficile DSM 1296(T) have been cloned and expressed in Escherichia coli. The enzymes acetyl-coenzyme A (CoA) C-acetyltransferase, 3-hydroxybutyryl-CoA dehydrogenase, crotonase, phosphate butyryltransferase, and butyrate kinase and the butyryl-CoA dehydrogenase complex composed o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00321-13
更新日期:2013-08-01 00:00:00
abstract::The twisting and writhing during growth of single-cell filaments of Bacillus subtilis which lead to macrofiber formation was studied in both left- and right-handed forms of strains FJ7 and RHX. Filament bending, touching, and loop formation (folding), followed by winding up into a double-strand fiber, were documented....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.24.7060-7069.1995
更新日期:1995-12-01 00:00:00
abstract::Escherichia coli lactose permease mediates the proton-driven translocation of galactosides across the cytoplasmic membrane. To define regions important for membrane insertion as well as for biological function, we constructed plasmids encoding different portions of the lactose carrier. Among several lacY deletions, tw...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.9.5374-5381.1990
更新日期:1990-09-01 00:00:00
abstract::Based on its genome sequence, the pathway of beta-oxidative fatty acid degradation in Salmonella enterica serovar Typhimurium LT2 has been thought to be identical to the well-characterized Escherichia coli K-12 system. We report that wild-type strains of S. enterica grow on decanoic acid, whereas wild-type E. coli str...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.188.2.599-608.2006
更新日期:2006-01-01 00:00:00
abstract::We measured some of the kinetic parameters of nitrogenase to intact systems of Clostridium pasteurianum and Klebsiella pneumoniae to compare them with the kinetics of the enzyme in vitro. We found that the enzyme showed multiple apparent Km values for acetylene reduction in vivo, as it does in vitro. Carbon monoxide w...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.141.3.1230-1238.1980
更新日期:1980-03-01 00:00:00
abstract::Lectin activity in a host-mycoparasite relationship was demonstrated with Rhizoctonia solani and Trichoderma harzianum. Attachment of O but not A and B erythrocytes to hyphae occurred on R. solani but not on its mycoparasite. This phenomenon, which was Ca2+ and Mn2+ dependent, was prevented by galactose, present in T....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.154.3.1431-1435.1983
更新日期:1983-06-01 00:00:00
abstract::Graber, C. D. (Baylor University College of Medicine, Houston, Tex.), R. M. O'Neal, and E. R. Rabin. Effect of high fat diets on intestinal microflora and serum cholesterol in rats. J. Bacteriol. 89:47-51. 1965.-Differential bacterial counts of feces and total plasma cholesterol determinations were performed on 60 Wis...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.89.1.47-51.1965
更新日期:1965-01-01 00:00:00
abstract::Bacillus licheniformis strain 749/C (constitutive for penicillinase formation) and uninduced cells of strain 749 (penicillinase-inducible) were examined after freezeetching. In the early stationary phase, strain 749/C organisms had clusters of vesicles (30 to 40 nm in diameter) on the outer surface of the plasma membr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.100.2.1002-1009.1969
更新日期:1969-11-01 00:00:00
abstract::The nucleotide sequence of a 7.3-kilobase-pair fragment of DNA encoding a hemolytic activity from Serratia marcescens was determined. Two large open reading frames were identified, designated shlA (Serratia hemolysin) and shlB, capable of encoding polypeptides of 165, 056 and 61,897 molecular weight, respectively. Bot...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.7.3177-3188.1988
更新日期:1988-07-01 00:00:00
abstract::We have cloned three copies of a repeated DNA segment from Borrelia burgdorferi sensu stricto strain B31, present on both circular and linear plasmids of this and other B. burgdorferi sensu lato strains. The DNA sequences are characterized by a highly homologous segment containing two open reading frames (ORFs), ORF-A...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.8.2287-2298.1996
更新日期:1996-04-01 00:00:00
abstract::Calcium carbonate precipitation, a widespread phenomenon among bacteria, has been investigated due to its wide range of scientific and technological implications. Nevertheless, little is known of the molecular mechanisms by which bacteria foster calcium carbonate mineralization. In our laboratory, we are studying calc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01450-06
更新日期:2007-01-01 00:00:00
abstract::Expression of the Aspergillus nidulans penicillin biosynthesis genes acvA and ipnA, encoding delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase and isopenicillin N synthetase, respectively, was analyzed. The intergenic region carrying the divergently oriented promoters was fused in frame in both orientations ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.11.3789-3799.1992
更新日期:1992-06-01 00:00:00
abstract::Cotransformation frequencies of 16, 39, 51, and 60% were observed when donor alleles were separated by distances of 9.2, 7.4, 6.3, and 5.1 kb, respectively, in donor Acinetobacter calcoaceticus DNA. A different and unexpected pattern was observed when the distance between recipient alleles was reduced from 9.2 to 5.1 ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.20.6015-6017.1995
更新日期:1995-10-01 00:00:00
abstract::During the growth of Clostridium cellulolyticum in chemostat cultures with ammonia as the growth-limiting nutrient, as much as 30% of the original cellobiose consumed by C. cellulolyticum was converted to cellotriose, glycogen, and polysaccharides regardless of the specific growth rates. Whereas the specific consumpti...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.7.2010-2017.2000
更新日期:2000-04-01 00:00:00
abstract::The fruK gene encoding fructose-1-phosphate kinase (FruK), located within the fructose (fru)-catabolic operon of Rhodobacter capsulatus, was sequenced. FruK of R. capsulatus (316 amino acids; molecular weight = 31,232) is the same size as and is homologous to FruK of Escherichia coli, phosphofructokinase B (PfkB) of E...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.10.3117-3127.1991
更新日期:1991-05-01 00:00:00
abstract::Deoxyribonucleic acid repair synthesis was studied in one wild-type and two mutant strains of Bacillus subtilis that are defective in excision of pyrimidine dimers. The cells were irradiated with ultraviolet light, and 6-(p-hydroxyphenyl-azo)-uracil was used to block replicative synthesis, allowing only repair synthes...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.139.1.239-246.1979
更新日期:1979-07-01 00:00:00
abstract::An increased demand for cytochromes is associated with symbiotic development and microaerobic metabolism in the bacterium Bradyrhizobium japonicum, and evidence suggests that hemB, rather than hemA, is the first essential bacterial heme synthesis gene in symbiosis with soybean. Steady-state levels of mRNA and protein ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.11.3706-3710.1997
更新日期:1997-06-01 00:00:00
abstract::Western (immunoblot) analysis using Escherichia coli anti-RecA antiserum revealed that expression of the RecA protein of Pseudomonas aeruginosa PAO is induced upon exposure of the bacterium to UV irradiation or norfloxacin, a quinolone related to nalidixic acid. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.5.2385-2387.1988
更新日期:1988-05-01 00:00:00
abstract::Growth of Agrobacterium tumefaciens on d-glucuronic acid (GlcUA) or d-galacturonic acid (GalUA) induces formation of hexuronic acid dehydrogenase [d-aldohexuronic acid: nicotinamide adenine dinucleotide (NAD) oxidoreductase]. The dehydrogenase, which irreversibly converts GlcUA or GalUA to the corresponding hexaric ac...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.99.3.667-673.1969
更新日期:1969-09-01 00:00:00
abstract::Schwartz, Norman M. (Yale University, New Haven, Conn.). Genetic instability in Escherichia coli. J. Bacteriol. 89:712-717. 1965.-Results obtained from a transductional analysis demonstrated that an extremely unstable reversion of lac(-) is a suppressor mutation. This mutation suppresses 2 of 22 different lac(-) allel...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.89.3.712-717.1965
更新日期:1965-03-01 00:00:00
abstract::Nine acetotrophic and three methylotrophic strains of methane-producing bacteria were screened for the presence of plasmid DNA. Plasmids were detected in three marine isolates, including Methanosarcina acetivorans. All three plasmids appeared to be similar based on size and restriction site analyses. The plasmid from ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.10.4979-4982.1988
更新日期:1988-10-01 00:00:00
abstract::The RimM protein in Escherichia coli is associated with free 30S ribosomal subunits but not with 70S ribosomes. A DeltarimM mutant shows a sevenfold-reduced growth rate and a reduced translational efficiency, probably as a result of aberrant assembly of the ribosomal 30S subunits. The slow growth and translational def...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.20.6095-6106.2001
更新日期:2001-10-01 00:00:00