Plasmid DNA from the acetotrophic methanogen Methanosarcina acetivorans.

abstract：:We have cloned a lipopolysaccharide (LPS) biosynthetic gene from Pseudomonas aeruginosa PAO1 that complements the defect in the production and incorporation of LPS O side chains in the LPS-rough strain AK1012. This gene was characterized by pulsed-field gel electrophoresis, deletion and restriction mapping of the clon...

journal_title：Journal of bacteriology

authors： Goldberg JB,Hatano K,Pier GB

更新日期：1993-03-01 00:00:00

abstract：:The lipid A components of substrate cell origin incorporated by Bdellovibrio bacteriovorus during intraperiplasmic growth (D. R. Nelson and S. C. Rittenberg, J. Bacteriol. 147:860-868, 1981) were shown to be integrated into its lipopolysaccharide structure. Lipid A isolated from bdellovibrios grown on Escherichia coli...

journal_title：Journal of bacteriology

authors： Nelson DR,Rittenberg SC

更新日期：1981-09-01 00:00:00

abstract：:Mutants with alterations in the structure, biosynthesis, or assembly of murein lipoprotein were selected by a procedure based on radiation suicide of wild-type organisms by [3H]arginine under conditions where the radioactive arginine was preferentially incorporated into lipoprotein. Further screening for the potential...

journal_title：Journal of bacteriology

authors： Wu HC,Lin JJ

更新日期：1976-04-01 00:00:00

abstract：:Chronic lung infections in cystic fibrosis (CF) could be treated more effectively if the effects of antimicrobials on pathogens in situ were known. Here, we compared changes in the microbial community composition and pathogen growth rates in longitudinal studies of seven pediatric CF patients undergoing intravenous an...

journal_title：Journal of bacteriology

authors： Neubauer C,Kasi AS,Grahl N,Sessions AL,Kopf SH,Kato R,Hogan DA,Newman DK

更新日期：2018-11-26 00:00:00

abstract：:The operons of the glp regulon encoding the glycerol metabolic enzymes of Pseudomonas aeruginosa were hitherto believed to be positively regulated by the product of the glpR regulatory gene. During nucleotide sequence analysis of the region located upstream of the previously characterized glpD gene, encoding sn-glycer...

journal_title：Journal of bacteriology

authors： Schweizer HP,Po C

更新日期：1996-09-01 00:00:00

abstract：:Clinical isolates of the porcine pathogen Actinobacillus pleuropneumoniae often form adherent colonies on agar plates due to expression of an operon, pgaABCD, encoding a poly-beta-1,6-N-acetyl-D-glucosamine (PGA) extracellular matrix. The adherent colony phenotype, which correlates with the ability to form biofilms on...

journal_title：Journal of bacteriology

authors： Bossé JT,Sinha S,Li MS,O'Dwyer CA,Nash JH,Rycroft AN,Kroll JS,Langford PR

更新日期：2010-05-01 00:00:00

abstract：:Rhodospirillum rubrum was shown to grow fermentatively on fructose with N2 as a nitrogen source. The nitrogenase activity of these cells was regulated by the NH4+ switch-off/switch-on mechanism in a manner identical to that for photosynthetically grown cells. In vitro, the inactive nitrogenase Fe protein from fermenti...

journal_title：Journal of bacteriology

authors： Schultz JE,Gotto JW,Weaver PF,Yoch DC

更新日期：1985-06-01 00:00:00

abstract：:Fukuyama T. T. (University of Pennsylvania, Philadelphia) and D. J. O'Kane. Galactose metabolism. I. Pathway of carbon in fermentation by Streptococcus faecalis. J. Bacteriol. 84:793-796. 1962.-The pathway by which galactose-1-C(14) is fermented in Streptococcus faecalis was investigated using dried-cell preparations....

journal_title：Journal of bacteriology

authors： FUKUYAMA TT,O'KANE DJ

更新日期：1962-10-01 00:00:00

abstract：:Billen, Daniel (University of Texas M. D. Anderson Hospital and Tumor Institute, Houston, Tex.), and Roger Hewitt. Influence of starvation for methionine and other amino acids on subsequent bacterial deoxyribonucleic acid replication. J. Bacteriol. 92:609-617. 1966.-A study has been made of the subsequent replicative ...

journal_title：Journal of bacteriology

authors： Billen D,Hewitt R

更新日期：1966-09-01 00:00:00

abstract：:The Escherichia coli beta-galactosidase enzyme was used as a reporter molecule for genetic fusions in Rhodobacter capsulatus. DNA fragments that were from the upstream region of the hydrogenase structural operon hupSLM and contained 5' hupS sequences were fused in frame to a promoterless lacZ gene, yielding fusion pro...

journal_title：Journal of bacteriology

authors： Colbeau A,Vignais PM

更新日期：1992-07-01 00:00:00

abstract：:Microbial communities in the termite hindgut are essential for degrading plant material. We present the high-quality draft genome sequence of the Opitutaceae bacterium strain TAV1, the first member of the phylum Verrucomicrobia to be isolated from wood-feeding termites. The genomic analysis reveals genes coding for li...

journal_title：Journal of bacteriology

authors： Isanapong J,Goodwin L,Bruce D,Chen A,Detter C,Han J,Han CS,Held B,Huntemann M,Ivanova N,Land ML,Mavromatis K,Nolan M,Pati A,Pennacchio L,Pitluck S,Szeto E,Tapia R,Woyke T,Rodrigues JL

更新日期：2012-05-01 00:00:00

abstract：:The Ssa subfamily of Hsp70 molecular chaperones in the budding yeast Saccharomyces cerevisiae has four members, encoded by SSA1, SSA2, SSA3, and SSA4. Deletion of the two constitutively expressed genes, SSA1 and SSA2, results in cells which are slow growing and temperature sensitive. In this study, we demonstrate that...

journal_title：Journal of bacteriology

authors： Baxter BK,Craig EA

更新日期：1998-12-01 00:00:00

abstract：:Drugs known to interrupt the chain of protein synthesis (streptomycin, chloramphenicol, ethidium bromide) appeared to affect production of the group D lysin by Streptococcus zymogenes by inhibiting growth. Drugs which block cell wall synthesis (vancomycin, bacitracin, d-cycloserine, and phosphonomycin) inhibited lytic...

journal_title：Journal of bacteriology

authors： Werth JM,Jackson RW

更新日期：1971-11-01 00:00:00

abstract：:The nature of the phospholipids of the various bacteria that have ice nucleation activity in supercooled water has been determined. The seven bacteria studied included Pseudomonas syringae, Erwinia herbicola, three Escherichia coli K-12 strains that are phenotypically Ice+ because they contain plasmids with different ...

journal_title：Journal of bacteriology

authors： Kozloff LM,Turner MA,Arellano F,Lute M

更新日期：1991-03-01 00:00:00

abstract：:In a previous study, we showed that in bacteroids, transcription of the inducible nod genes does not occur and expression of nodD is decreased by 65% (H. R. M. Schlaman, B. Horvath, E. Vijgenboom, R.J.H. Okker, and B. J. J. Lugtenberg, J. Bacteriol. 173:4277-4287, 1991). In the present study, we show, using gel retard...

journal_title：Journal of bacteriology

authors： Schlaman HR,Lugtenberg BJ,Okker RJ

更新日期：1992-10-01 00:00:00

abstract：:In many microorganisms the first step for alkane degradation is the terminal oxidation of the molecule by an alkane hydroxylase. We report the characterization of a gene coding for an alkane hydroxylase in a Burkholderia cepacia strain isolated from an oil-contaminated site. The protein encoded showed similarity to ot...

journal_title：Journal of bacteriology

authors： Marín MM,Smits TH,van Beilen JB,Rojo F

更新日期：2001-07-01 00:00:00

abstract：:Streptococcus thermophilus strain CNRZ 302 is unable to ferment galactose, neither that generated intracellularly by lactose hydrolysis nor the free sugar. Nevertheless, sequence analysis and complementation studies with Escherichia coli demonstrated that strain CNRZ 302 contained structurally intact genes for the Lel...

journal_title：Journal of bacteriology

authors： Vaughan EE,van den Bogaard PT,Catzeddu P,Kuipers OP,de Vos WM

更新日期：2001-02-01 00:00:00

abstract：:The effect of CheY and fumarate on switching frequency and rotational bias of the bacterial flagellar motor was analyzed by computer-aided tracking of tethered Escherichia coli. Plots of cells overexpressing CheY in a gutted background showed a bell-shaped correlation curve of Switching frequency and bias centering at...

journal_title：Journal of bacteriology

authors： Montrone M,Eisenbach M,Oesterhelt D,Marwan W

更新日期：1998-07-01 00:00:00

abstract：:With two-dimensional restriction enzyme analysis we have been able to cleave the Bacillus subtilis genome and resolve the resulting deoxyribonucleic acid (DNA) segments into discrete bands on agarose gels. A general procedure for gene purification has been developed by coupling multidimensional restriction analysis wi...

journal_title：Journal of bacteriology

authors： Potter SS,Bott KF,Newbold JE

更新日期：1977-01-01 00:00:00

abstract：:Quorum sensing is involved in the regulation of multicellular behavior through communication via small molecules. Given the high number and diversity of the gastrointestinal microbiota, it is postulated that members of this community communicate to coordinate a variety of adaptive processes. AI-2 is suggested to be a ...

journal_title：Journal of bacteriology

authors： Lebeer S,De Keersmaecker SC,Verhoeven TL,Fadda AA,Marchal K,Vanderleyden J

更新日期：2007-02-01 00:00:00

abstract：:Cell wall turnover was examined in parent and mutant strains of Staphylococcus aureus. Peptidoglycan and teichoic acid were observed to undergo turnover in the wild-type strain during exponential growth; however, the rate of turnover did not decrease when the growth rate slowed, as the culture entered stationary phase...

journal_title：Journal of bacteriology

authors： Wong W,Chatterjee AN,Young FE

更新日期：1978-05-01 00:00:00

abstract：:By means of electron microscope autoradiography of component cultures of Bacillus subtilis exposed to [(3)H]thymidine-labeled transforming deoxyribonucleic acid competent and noncompetent cells can be distinguished. Competence is not limited to a specific phase of the cell division cycle. With serial section electron ...

journal_title：Journal of bacteriology

authors： Vermeulen CA,Venema G

更新日期：1974-05-01 00:00:00

abstract：:The aim of this study was to identify the compatible solutes accumulated by Pseudomonas putida S12 subjected to osmotic stress. In response to reduced water activity, P. putida S12 accumulated Nalpha-acetylglutaminylglutamine amide (NAGGN) simultaneously with a novel compatible solute identified as mannitol (using 13C...

journal_title：Journal of bacteriology

authors： Kets EP,Galinski EA,de Wit M,de Bont JA,Heipieper HJ

更新日期：1996-12-01 00:00:00

abstract：:Mutants of Escherichia coli capable of growing in the presence of 10 microgram of mecillinam per ml were selected after intensive mutagenesis. Of these mutants, 1.4% formed normal, rod-shaped cells at 30 degrees C but grew as spherical cells at 42 degrees C. The phenotype of one of these rod(Ts) mutants was 88% cotran...

journal_title：Journal of bacteriology

authors： Iwaya M,Goldman R,Tipper DJ,Feingold B,Strominger JL

更新日期：1978-12-01 00:00:00

abstract：:The DnaA protein concentration in Escherichia coli was increased above the wild-type level by inducing a lacP-controlled dnaA gene located on a plasmid. In these cells with different DnaA protein levels, we measured several parameters: dnaA gene expression; cell size, amount of DNA per cell, and number of origins per ...

journal_title：Journal of bacteriology

authors： Atlung T,Hansen FG

更新日期：1993-10-01 00:00:00

abstract：:Formylglutamate amidohydrolase (FGase) catalyzes the terminal reaction in the five-step pathway for histidine utilization in Pseudomonas putida. By this action, N-formyl-L-glutamate (FG) is hydrolyzed to produce L-glutamate plus formate. Urocanate, the first product in the pathway, induced all five enzymes, but FG was...

journal_title：Journal of bacteriology

authors： Hu L,Mulfinger LM,Phillips AT

更新日期：1987-10-01 00:00:00

abstract：:The chromatophores of Chromatium vinosum, as well as six other photosynthetic bacteria, contained two or more proteins which were insoluble when heated in the presence of sodium dodecyl sulfate (SDS) and 2-mercaptoethanol (beta-ME). When the chromatophores were dissolved at room temperature in SDS-beta-ME, these prote...

journal_title：Journal of bacteriology

authors： Hui KM,Hurlbert RE

更新日期：1979-04-01 00:00:00

abstract：:The ability of Clostridium perfringens type A to produce an enterotoxin active in human food poisoning has been shown to be directly related to the ability of the organism to sporulate. Enterotoxin was produced only in a sporulation medium and not in a growth medium in which sporulation was repressed. Mutants with an ...

journal_title：Journal of bacteriology

authors： Duncan CL,Strong DH,Sebald M

更新日期：1972-04-01 00:00:00

abstract：:The yeast metallothionein gene CUP1 was cloned into a bacterial expression system to achieve efficient, controlled expression of the stable, unprocessed protein product. The Escherichia coli-synthesized yeast metallothionein bound copper, cadmium, and zinc, indicating that the protein was functional. Furthermore, E. c...

journal_title：Journal of bacteriology

authors： Berka T,Shatzman A,Zimmerman J,Strickler J,Rosenberg M

更新日期：1988-01-01 00:00:00

abstract：:The bacterium Myxococcus xanthus undergoes multicellular development during times of nutritional stress and uses extracellular signals to coordinate cell behavior. C-signal affects gene expression late in development, including that of Omega4499, an operon identified by insertion of Tn5 lac into the M. xanthus chromos...

journal_title：Journal of bacteriology

authors： Yoder DR,Kroos L

更新日期：2004-06-01 00:00:00