Modular organization of the Phd repressor/antitoxin protein.

abstract：:An 8,883-bp mini-pXO1 plasmid containing a replicon from Bacillus anthracis pXO1 (181.6 kb) was identified by making large deletions in the original plasmid using a newly developed Cre-loxP system. Portions of the truncated mini-pXO1 were cloned into an Escherichia coli vector unable to replicate in B. anthracis. A 5....

journal_title：Journal of bacteriology

authors： Pomerantsev AP,Camp A,Leppla SH

更新日期：2009-08-01 00:00:00

abstract：:We have developed a rapid and sensitive fluorimetric method, based on the formation of a fluorescent product from nitrosation of 2,3-diaminonaphthalene, for measuring the ability of bacteria to catalyze nitrosation of amines. We have shown in Escherichia coli that nitrosation can be induced under anaerobic conditions ...

journal_title：Journal of bacteriology

authors： Ralt D,Wishnok JS,Fitts R,Tannenbaum SR

更新日期：1988-01-01 00:00:00

abstract：:The expression of the positively charged human protein secretory leukocyte protease inhibitor (SLPI) in Escherichia coli causes severe cellular toxicity. After induction of SLPI synthesis in a high-level-expression strain, SGE61, the growth of the strain is arrested and total protein and RNA synthesis rates decline by...

journal_title：Journal of bacteriology

authors： Miller KW,Evans RJ,Eisenberg SP,Thompson RC

更新日期：1989-04-01 00:00:00

abstract：:SigF is an alternative sigma factor that is highly conserved among species of the genus Mycobacterium. In this study we identified the SigF regulon in Mycobacterium smegmatis using whole-genome microarray and promoter consensus analyses. In total, 64 genes in exponential phase and 124 genes in stationary phase are Sig...

journal_title：Journal of bacteriology

authors： Hümpel A,Gebhard S,Cook GM,Berney M

更新日期：2010-05-01 00:00:00

abstract：:Streptococcus pneumoniae is the causative agent of multiple diseases, including otitis media, pneumonia, bacteremia, and meningitis. Pneumolysin (Ply), a member of the cholesterol-dependent cytolytic pore-forming toxins, is produced by virtually all clinical isolates of S. pneumoniae, and strains in which the Ply gene...

journal_title：Journal of bacteriology

authors： Price KE,Camilli A

更新日期：2009-04-01 00:00:00

abstract：:We have used a plasmid-based transcriptional reporter system to examine the transcriptional effects of 33 single point mutations in the box A region (TATA-like sequence) of the Haloferax volcanii tRNA(Lys) promoter. The most pronounced effects on transcriptional efficiency were found when the nucleotides corresponding...

journal_title：Journal of bacteriology

authors： Palmer JR,Daniels CJ

更新日期：1995-04-01 00:00:00

abstract：:Anaerobic growth of Klebsiella aerogenes NCDO 711 (NCTC 418) on citrate was dependent on the presence of Na+ in the medium, and fermentation of citrate was mediated via the fermentation pathway enzymes, citrate lyase and a Na+-dependent oxalacetate decarboxylase. This confirms the previous findings on strain NCTC 418....

journal_title：Journal of bacteriology

authors： O'Brien RW

更新日期：1975-05-01 00:00:00

abstract：:The biological activities of Bacillus subtilis ribosomes pretreated with 0.5, 1, or 2 mmp-chloromercuribenzoate (PCMB) were examined. The ribosomes treated with 1 or 2 mm PCMB lost their capacity to synthesize polyphenylalanine as well as the capacity to bind phenylalanyl-transfer ribonucleic acid. On the other hand, ...

journal_title：Journal of bacteriology

authors： Ranu RS,Kaji A

更新日期：1972-10-01 00:00:00

abstract：:Diploid cells of Saccharomyces cerevisiae underwent meiosis and sporulation when placed in 1% potassium acetate sporulation medium. In unbuffered sporulation medium the pH rose very rapidly, reaching pH 8.4 after 2 h of sporulation. Under these conditions, the uptake of radioactive adenine and lysine was extremely lim...

journal_title：Journal of bacteriology

authors： McCusker JH,Haber JE

更新日期：1977-10-01 00:00:00

abstract：:The radB101 and recN262 mutations showed essentially identical phenotypes when compared in isogenic Escherichia coli strains for their effects on gamma and UV radiation survival and on conjugal recombination in a uvrA recB recC sbcB sbcC strain. Complementation tests involving attempts to reconstitute a radB+ recN+ st...

journal_title：Journal of bacteriology

authors： Sargentini NJ,Smith KC

更新日期：1988-05-01 00:00:00

abstract：:Both Salmonella enterica serovar Typhimurium and Escherichia coli contain the cspH gene encoding CspH, one of the cold shock proteins (CSPs). In this study, we investigated the expression of cspH in S. enterica serovar Typhimurium and found that it was induced in response to a temperature downshift during exponential ...

journal_title：Journal of bacteriology

authors： Kim BH,Bang IS,Lee SY,Hong SK,Bang SH,Lee IS,Park YK

更新日期：2001-10-01 00:00:00

abstract：:Stocks, Peter K. (Louisiana State University, Baton Rouge), and C. S. McCleskey. Identity of the pink-pigmented methanol-oxidizing bacteria as Vibrio extorquens. J. Bacteriol. 88:1065-1070. 1964.-Pink-pigmented bacteria isolated from enrichment cultures of methane oxidizers were found to possess similar morphological,...

journal_title：Journal of bacteriology

authors： STOCKS PK,MCCLESKEY CS

更新日期：1964-10-01 00:00:00

abstract：:C(1), a lytic bacteriophage infecting group C streptococci, is one of the earliest-isolated phages, and the method of bacterial classification known as phage typing was defined by using this bacteriophage. We present for the first time a detailed analysis of this phage by use of electron microscopy, protein profiling,...

journal_title：Journal of bacteriology

authors： Nelson D,Schuch R,Zhu S,Tscherne DM,Fischetti VA

更新日期：2003-06-01 00:00:00

abstract：:The fatty acid composition of the membranes of three different penicillin-produced L-forms of Streptococcus faecalis was determined: (i) a stable (nonreverting) L-form (T(53)) cultured in brain heart infusion (BHI) with 0.5 M sucrose; (ii) a stable L-form (T(531)) cultured in BHI without sucrose; and (iii) an unstable...

journal_title：Journal of bacteriology

authors： Montgomerie JZ,Kalmanson GM,Guze LB

更新日期：1973-07-01 00:00:00

abstract：:Cells of the osmotolerant yeast Saccharomyces rouxii were transformed to protoplasts in good yield (85%) by digesting cell walls with snail-gut enzyme in the presence of 10 mM dithioerythritol, 0.1 M sodium phosphate buffer (pH 6.8), and 2.0 M KCl. The requirement for 2.0 M KCl compares with that for S. bisporus var. ...

journal_title：Journal of bacteriology

authors： Arnold WN,Garrison RG

更新日期：1979-03-01 00:00:00

abstract：:The galP1 promoter is responsible for galactose-dependent, glucose-sensitive transcription of the galactose utilization operon of Streptomyces coelicolor and Streptomyces lividans. We describe the characterization of mutations that were positioned directly upstream of the apparent transcription start site of galP1 and...

journal_title：Journal of bacteriology

authors： Mattern SG,Brawner ME,Westpheling J

更新日期：1993-03-01 00:00:00

abstract：:The sequence of the pckA gene coding for phosphoenolpyruvate carboxykinase in Escherichia coli K-12 and previous molecular weight determinations indicate that this allosteric enzyme is a monomer of Mr 51,316. The protein is homologous to ATP-dependent phosphoenolpyruvate carboxykinases from Trypanosoma brucei and Sacc...

journal_title：Journal of bacteriology

authors： Medina V,Pontarollo R,Glaeske D,Tabel H,Goldie H

更新日期：1990-12-01 00:00:00

abstract：:Different preparations of the methylreductase were tested in a simplified methylcoenzyme M methylreductase assay with artificial electron donors under a nitrogen atmosphere. ATP and Mg2+ stimulated the reaction. Tris(2,2'-bipyridine)ruthenium (II), chromous chloride, chromous acetate, titanium III citrate, 2,8-diamino...

journal_title：Journal of bacteriology

authors： Hartzell PL,Escalante-Semerena JC,Bobik TA,Wolfe RS

更新日期：1988-06-01 00:00:00

abstract：:Recently we identified a bacterial factor (BimA) required for actin-based motility of Burkholderia pseudomallei. Here we report that Burkholderia mallei and Burkholderia thailandensis are capable of actin-based motility in J774.2 cells and that BimA homologs of these bacteria can restore the actin-based motility defec...

journal_title：Journal of bacteriology

authors： Stevens JM,Ulrich RL,Taylor LA,Wood MW,Deshazer D,Stevens MP,Galyov EE

更新日期：2005-11-01 00:00:00

abstract：:Utilizing a homologous gene replacement in order to retain the native promoter and 5' and 3' untranslated messenger regions (and thereby ensure physiological validity), we have shown that deletion of the N-terminal 389 amino acids of the transcriptional activator AREA does not result in nitrogen metabolite derepressio...

journal_title：Journal of bacteriology

authors： Caddick MX,Arst HN Jr

更新日期：1998-11-01 00:00:00

abstract：:Resting cells of Escherichia coli are able to initiate growth and murein biosynthesis in the presence of beta-lactam antibiotics binding to penicillin-binding proteins (PBPs) 1a and 1b (E. J. de la Rosa, M. A. de Pedro, and D. Vázquez, Proc. Natl. Acad. Sci. USA 82:5632-5635, 1985). Under these conditions, cells elong...

journal_title：Journal of bacteriology

authors： García-del Portillo F,Pisabarro AG,de la Rosa EJ,de Pedro MA

更新日期：1987-06-01 00:00:00

abstract：:Supercoiled double-stranded DNA molecules (plasmids) were isolated from plants infected with three laboratory strains of western aster yellows mycoplasma-like organism (AY-MLO) by using cesium chloride-ethidium bromide density gradients. Southern blot analysis, using plasmids from the severe strain of AY-MLO (SAY-MLO)...

journal_title：Journal of bacteriology

authors： Kuske CR,Kirkpatrick BC

更新日期：1990-03-01 00:00:00

abstract：:The vir locus of Bordetella pertussis apparently encodes a trans-acting positive regulator that is required for the coordinate expression of genes associated with virulence: pertussis toxin, filamentous hemagglutinin (FHA), hemolysin, and adenylate cyclase toxin. DNA clones of vir and of genes required for the synthes...

journal_title：Journal of bacteriology

authors： Stibitz S,Weiss AA,Falkow S

更新日期：1988-07-01 00:00:00

abstract：:We have isolated three strains of Klebsiella aerogenes that failed to show repression of glutamine synthetase even when grown under the most repressing conditions for the wild-type strain. These mutant strains were selected as glutamine-independent derivatives of a strain that is merodiploid for the glnA region and co...

journal_title：Journal of bacteriology

authors： Rothstein DM,Magasanik B

更新日期：1980-02-01 00:00:00

abstract：:A Butyrivibrio fibrisolvens H17c glgB gene, was isolated by direct selection for colonies that produced clearing on starch azure plates. The gene was expressed in Escherichia coli from its own promoter. The glgB gene consisted of an open reading frame of 1,920 bp encoding a protein of 639 amino acids (calculated Mr, 7...

journal_title：Journal of bacteriology

authors： Rumbak E,Rawlings DE,Lindsey GG,Woods DR

更新日期：1991-11-01 00:00:00

abstract：:Nonpiliated, phage phi 6-resistant mutants of Pseudomonas syringae pv. phaseolicola were generated by Tn5 transposon mutagenesis. A P. syringae pv. phaseolicola LR700 cosmid library was screened with Tn5-containing EcoRI fragments cloned from nonpiliated mutants. The cosmid clone pVK253 complemented the nonpiliated mu...

journal_title：Journal of bacteriology

authors： Roine E,Nunn DN,Paulin L,Romantschuk M

更新日期：1996-01-01 00:00:00

abstract：:An unusual xylose isomerase produced by Thermoanaerobacterium strain JW/SL-YS 489 was purified 28-fold to gel electrophoretic homogeneity, and the biochemical properties were determined. Its pH optimum distinguishes this enzyme from all other previously described xylose isomerases. The purified enzyme had maximal acti...

journal_title：Journal of bacteriology

authors： Liu SY,Wiegel J,Gherardini FC

更新日期：1996-10-01 00:00:00

abstract：:The operons of the glp regulon encoding the glycerol metabolic enzymes of Pseudomonas aeruginosa were hitherto believed to be positively regulated by the product of the glpR regulatory gene. During nucleotide sequence analysis of the region located upstream of the previously characterized glpD gene, encoding sn-glycer...

journal_title：Journal of bacteriology

authors： Schweizer HP,Po C

更新日期：1996-09-01 00:00:00

abstract：:Two types of adhesive fimbriae are expressed by Actinomyces; however, the architecture and the mechanism of assembly of these structures remain poorly understood. In this study we characterized two fimbrial gene clusters present in the genome of Actinomyces naeslundii strain MG-1. By using immunoelectron microscopy an...

journal_title：Journal of bacteriology

authors： Mishra A,Das A,Cisar JO,Ton-That H

更新日期：2007-04-01 00:00:00

abstract：:The uropathogenic Escherichia coli strain 536 carries at least five genetic elements on its chromosome that meet all criteria characteristic of pathogenicity islands (PAIs). One main feature of these distinct DNA regions is their instability. We applied the so-called island-probing approach and individually labeled al...

journal_title：Journal of bacteriology

authors： Middendorf B,Hochhut B,Leipold K,Dobrindt U,Blum-Oehler G,Hacker J

更新日期：2004-05-01 00:00:00