Abstract:
:Expression of fimA, the structural gene for type 1 fimbriae of Escherichia coli, is phase variable. Significant homologies were identified between the recombinases which control fimbrial phase variation, FimB and FimE, and the integrase class of site-specific recombinases. Normal expression of fimA was shown to require the integration host factor (IHF). Mutations in either the himA-or the himD (hip) gene, which encode the alpha and beta subunits of IHF, respectively, prevented phase variation and locked expression of fimA in either the "on" or "off" phase. In addition, both himA and himD lesions caused a sevenfold reduction in expression of a phi(fimA-lacZ) operon fusion in strains in which fimA was locked in the on phase. Thus, IHF plays a dual role in controlling fimA expression: it is required both for inversion of the fimA control region and for efficient expression from the fimA promoter. A mechanism by which IHF may exert control over fimA expression is discussed.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Dorman CJ,Higgins CFdoi
10.1128/jb.169.8.3840-3843.1987subject
Has Abstractpub_date
1987-08-01 00:00:00pages
3840-3issue
8eissn
0021-9193issn
1098-5530journal_volume
169pub_type
杂志文章abstract::The enzyme pattern of Saccharomyces cerevisiae was followed during batch growth and in continuous culture in a synthetic medium limited for glucose under aerobic conditions. Seven enzymes were measured: succinate-cytochrome c oxidoreductase, malate dehydrogenase, nicotinamide adenine dinucleotide-linked glutamate dehy...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.96.2.479-486.1968
更新日期:1968-08-01 00:00:00
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.113.3.1363-1372.1973
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.7.3620-3630.1990
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.107.1.295-302.1971
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doi:10.1128/jb.177.3.580-585.1995
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doi:10.1128/JB.123.2.755-758.1975
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doi:10.1128/JB.181.14.4237-4244.1999
更新日期:1999-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.12.3674-3680.1999
更新日期:1999-06-01 00:00:00
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.165.3.837-842.1986
更新日期:1986-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:1993-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:1973-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.11.3429-3438.1992
更新日期:1992-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2007-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.10.2878-2886.1995
更新日期:1995-05-01 00:00:00