Characterization of novel yeast RAD6 (UBC2) ubiquitin-conjugating enzyme mutants constructed by charge-to-alanine scanning mutagenesis.

abstract：:A lambda phage (lambdaNK55) carrying the translocatable element Tn10, conferring tetracycline resistance (Tetr), has been utilized to isolate glutamine auxotrophs of Escherichia coli K-12. Such strains lack uridylyltransferase as a result of an insertion of the TN10 element in the glnD gene. The glnD::Tn10 insertion h...

journal_title：Journal of bacteriology

authors： Bloom FR,Levin MS,Foor F,Tyler B

更新日期：1978-05-01 00:00:00

abstract：:Thiamine pyrophosphate (TPP), the biologically active form of thiamine (also known as vitamin B1), is an essential cofactor for several important enzymes involved in carbohydrate metabolism, and therefore, it is required for all living organisms. We recently found that a thiamine-binding protein (TDE_0143) is essentia...

journal_title：Journal of bacteriology

authors： Bian J,Tu Y,Wang SM,Wang XY,Li C

更新日期：2015-04-01 00:00:00

abstract：:Korormicin is an antibiotic produced by some pseudoalteromonads which selectively kills Gram-negative bacteria that express the Na+-pumping NADH:quinone oxidoreductase (Na+-NQR.) We show that although korormicin is an inhibitor of Na+-NQR, the antibiotic action is not a direct result of inhibiting enzyme activity. Ins...

journal_title：Journal of bacteriology

authors： Maynard A,Butler NL,Ito T,da Silva AJ,Murai M,Chen T,Koffas MAG,Miyoshi H,Barquera B

更新日期：2019-05-08 00:00:00

abstract：:In Rhodobacter sphaeroides, MreB, MreC, MreD, PBP2, and RodA are encoded at the same locus. The localizations of PBP2, MreB, and MreC, which have all been implicated in the synthesis of the peptidoglycan layer, were investigated under different growth conditions to gain insight into the relationships between these pro...

journal_title：Journal of bacteriology

authors： Slovak PM,Porter SL,Armitage JP

更新日期：2006-03-01 00:00:00

abstract：:Caulobacter is a distinctive genus of prosthecate bacteria. Because caulobacters adhere to surfaces and are found in diverse locales, their role in oligotrophic environments and bacterial biofilm communities is of interest. The phylogenetic relationships of a group of marine and freshwater caulobacters were examined i...

journal_title：Journal of bacteriology

authors： Stahl DA,Key R,Flesher B,Smit J

更新日期：1992-04-01 00:00:00

abstract：:The regulation of Agrobacterium tumefaciens heat shock genes involves a transcriptional activator (RpoH) and repressor elements (HrcA-CIRCE). Using proteome analysis and mutants in these control elements, we show that the heat shock induction of 32 (out of 56) heat shock proteins is independent of RpoH and HrcA. These...

journal_title：Journal of bacteriology

authors： Rosen R,Büttner K,Becher D,Nakahigashi K,Yura T,Hecker M,Ron EZ

更新日期：2002-03-01 00:00:00

abstract：:Archaea are abundant and drive critical microbial processes in the Earth's cold biosphere. Despite this, not enough is known about the molecular mechanisms of cold adaptation and no biochemical studies have been performed on stenopsychrophilic archaea (e.g., Methanogenium frigidum). This study examined the structural ...

journal_title：Journal of bacteriology

authors： Giaquinto L,Curmi PM,Siddiqui KS,Poljak A,DeLong E,DasSarma S,Cavicchioli R

更新日期：2007-08-01 00:00:00

abstract：:Dowell, C. E. (The University of Texas, Dallas) and E. D. Rosenblum. Staphylococcal transducing particle. J. Bacteriol. 84:1076-1079. 1962.-When novobiocin-resistant transductants were isolated under conditions that permitted superinfection, almost all the clones were lysogenic for the transducing phage. If superinfec...

journal_title：Journal of bacteriology

authors： Dowell CE,Rosenblum ED

更新日期：1962-11-01 00:00:00

abstract：:The levels of reduced and oxidized nicotinamide adenine dinucleotides were determined in Xanthobacter flavus during a transition from heterotrophic to autotrophic growth. Excess reducing equivalents are rapidly dissipated following induction of the Calvin cycle, indicating that the Calvin cycle serves as a sink for ex...

journal_title：Journal of bacteriology

authors： van Keulen G,Dijkhuizen L,Meijer WG

更新日期：2000-08-01 00:00:00

abstract：:Transcription of the trp operon of Bacillus subtilis is regulated by attenuation. A trpE'-'lacZ gene fusion preceded by the wild-type trp promoter-leader region was used to analyze regulation. Overproduction of the trp leader transcript in trans from a multicopy plasmid caused constitutive expression of the chromosoma...

journal_title：Journal of bacteriology

authors： Kuroda MI,Henner D,Yanofsky C

更新日期：1988-07-01 00:00:00

abstract：:By transformation of dnaA null mutant host cells that are suppressed either by an rnh mutation or by chromosomal integration of a mini-R1 plasmid, it was shown that replication of miniplasmids composed of the NR1 minimal replicon had no absolute dependence upon DnaA protein. In addition, the suppression of the dnaA nu...

journal_title：Journal of bacteriology

authors： Tang XB,Womble DD,Rownd RH

更新日期：1989-10-01 00:00:00

abstract：:Escherichia coli possesses three distinct formate dehydrogenase enzymes encoded by the fdnGHI, fdhF, and fdoGHI operons. To examine how two of the formate dehyrogenase operons (fdnGHI and fdhF) are expressed anaerobically in the presence of low, intermediate, and high levels of nitrate, nitrite, and formate, chemostat...

journal_title：Journal of bacteriology

authors： Wang H,Gunsalus RP

更新日期：2003-09-01 00:00:00

abstract：:Outer membrane preparations of Bdellovibrio bacteriovorus grown intraperiplasmically on Escherichia coli containing OmpF were prepared by the Triton X-100 procedure of Schnaitman (J. Bacteriol. 108:545-552, 1971). They contained a protein that migrated to almost the same position as E. coli OmpF in sodium dodecyl sulf...

journal_title：Journal of bacteriology

authors： Rayner JR,Cover WH,Martinez RJ,Rittenberg SC

更新日期：1985-08-01 00:00:00

abstract：:Arai, Tadashi (Chiba University, Chiba, Japan), Shyuko Kuroda, and Michio Ito. Possible utility of a fluorescent antibody technique in the serological identification of antagonistic Streptomyces. J. Bacteriol. 83:20-26. 1962.-The indirect fluorescent antibody technique was extended to the serological investigation of ...

journal_title：Journal of bacteriology

authors： ARAI T,KURODA S,ITO M

更新日期：1962-01-01 00:00:00

abstract：:Extracytoplasmic function σ factors that are stress inducible are often sequestered in an inactive complex with a membrane-associated anti-σ factor. Mycobacterium tuberculosis membrane-associated anti-σ factors have a small, stable RNA gene A (ssrA)-like degron for targeted proteolysis. Interaction between the unfolda...

journal_title：Journal of bacteriology

authors： Joshi AC,Kaur P,Nair RK,Lele DS,Nandicoori VK,Gopal B

更新日期：2019-02-25 00:00:00

abstract：:Salmonella typhimurium and Escherichia coli from anaerobic cultures displayed tactic responses to gradients of nitrate, fumarate, and oxygen when the appropriate electron transport pathway was present. Such responses were named "electron acceptor taxis" because they are elicited by terminal electron acceptors. Mutant ...

journal_title：Journal of bacteriology

authors： Taylor BL,Miller JB,Warrick HM,Koshland DE Jr

更新日期：1979-11-01 00:00:00

abstract：:Cell size at bud initiation shows the same relationship to growth rate in wild-type and petite yeast cells. The nutrient-modulated size control is related to growth rate rather than a change from fermentative to oxidative metabolism. ...

journal_title：Journal of bacteriology

authors： Bugeja VC,Carter BL

更新日期：1982-08-01 00:00:00

abstract：:The methanol-insoluble, heat-stable enterotoxin of Escherichia coli synthesized by clinical strains or strains that harbor the cloned gene was shown to be an extracellular polypeptide. The toxin (STB) was first detected as an 8,100-Mr precursor (pre-STB) that was converted to a transiently cell-associated 5,200-Mr for...

journal_title：Journal of bacteriology

authors： Kupersztoch YM,Tachias K,Moomaw CR,Dreyfus LA,Urban R,Slaughter C,Whipp S

更新日期：1990-05-01 00:00:00

abstract：:Colicin E2 was partially purified from Escherichia coli W3110. This preparation was remarkably stabilized by bovine serum albumin in a solution at neutral pH, as shown by dilution experiments and tests on heat stability of colicin. One killing unit of colicin E2 was estimated to correspond to one molecule of colicin E...

journal_title：Journal of bacteriology

authors： Mitsui E,Mizuno D

更新日期：1969-11-01 00:00:00

abstract：:Transcriptomic, metabolomic, physiological, and computational modeling approaches were integrated to gain insight into the mechanisms of antibiotic tolerance in an in vitro biofilm system. Pseudomonas aeruginosa biofilms were grown in drip flow reactors on a medium composed to mimic the exudate from a chronic wound. A...

journal_title：Journal of bacteriology

authors： Stewart PS,White B,Boegli L,Hamerly T,Williamson KS,Franklin MJ,Bothner B,James GA,Fisher S,Vital-Lopez FG,Wallqvist A

更新日期：2019-10-21 00:00:00

abstract：:Pseudomonas fluorescens strain KU-7 is a prototype microorganism that metabolizes 2-nitrobenzoate (2-NBA) via the formation of 3-hydroxyanthranilate (3-HAA), a known antioxidant and reductant. The initial two steps leading to the sequential formation of 2-hydroxy/aminobenzoate and 3-HAA are catalyzed by a NADPH-depend...

journal_title：Journal of bacteriology

authors： Iwaki H,Muraki T,Ishihara S,Hasegawa Y,Rankin KN,Sulea T,Boyd J,Lau PC

更新日期：2007-05-01 00:00:00

abstract：:DNA damage-inducible DNA replication in SOS-induced Escherichia coli cells, termed inducible stable DNA replication (iSDR), has previously been shown to require either the RecBCD or the RecE pathway of homologous recombination for initiation. Here, we demonstrate that recB recC sbcC quadruple mutant cells are capable ...

journal_title：Journal of bacteriology

authors： Asai T,Kogoma T

更新日期：1994-11-01 00:00:00

abstract：:Conti, S. F. (Dartmouth Medical School, Hanover, N.H.), and Peter Hirsch. Biology of budding bacteria. III. Fine structure of Rhodomicrobium and Hyphomicrobium spp. J. Bacteriol. 89:503-512. 1965.-The ultrastructure of 14 strains of hyphomicrobia, and of Rhodomicrobium vannielii, was investigated by means of electron ...

journal_title：Journal of bacteriology

authors： CONTI SF,HIRSCH P

更新日期：1965-02-01 00:00:00

abstract：:A macrorestriction map representing the complete physical map of the Rhodobacter sphaeroides 2.4.1 chromosomes has been constructed by ordering the chromosomal DNA fragments from total genomic DNA digested with the restriction endonucleases AseI, SpeI, DraI, and SnaBI. Junction fragments and multiple restriction endon...

journal_title：Journal of bacteriology

authors： Suwanto A,Kaplan S

更新日期：1989-11-01 00:00:00

abstract：:The WalRK two-component system plays important roles in maintaining cell wall homeostasis and responding to antibiotic stress in low-GC Gram-positive bacteria. In the major human pathogen, Streptococcus pneumoniae, phosphorylated WalR(Spn) (VicR) response regulator positively controls the transcription of genes encodi...

journal_title：Journal of bacteriology

authors： Gutu AD,Wayne KJ,Sham LT,Winkler ME

更新日期：2010-05-01 00:00:00

abstract：:A gene for the constitutive ribosephosphate isomerase (rpiA) is highly cotransducible with serA at 56.2 min on the genetic linkage map of Escherichia coli K-12. Suppression of ribosephosphate isomerase A-negative mutants can occur by a regulator gene mutation permitting constitutive synthesis of the normally inducible...

journal_title：Journal of bacteriology

authors： Skinner AJ,Cooper RA

更新日期：1974-06-01 00:00:00

abstract：:Regulation of isoleucine, valine, and leucine biosynthesis and isoleucyl-, valyl-, and leucyl-transfer ribonucleic acid (tRNA) synthetase formation was examined in two mutant strains of Escherichia coli. One mutant was selected for growth resistance to the isoleucine analogue, ketomycin, and the other was selected for...

journal_title：Journal of bacteriology

authors： Jackson J,Williams LS,Umbarger HE

更新日期：1974-12-01 00:00:00

abstract：:Agrobacterium tumefaciens wild-type strains have a unique quorum-sensing (QS)-dependent Ti plasmid conjugative transfer phenotype in which QS signaling is activated by corresponding conjugative opine inducers. Strain K588, with a nopaline-type chromosomal background harboring an octopine-type Ti plasmid, however, is a...

journal_title：Journal of bacteriology

authors： Wang C,Zhang HB,Chen G,Chen L,Zhang LH

更新日期：2006-04-01 00:00:00

abstract：:Sguros, Peter L. (University of Miami, Miami, Fla.), and Jacqueline Simms. Role of marine fungi in the biochemistry of the oceans. IV. Growth responses to seawater inorganic macroconstituents. J. Bacteriol. 88:346-355. 1964.-The filamentous ascomycete Halosphaeria mediosetigera and the deuteromycete Culcitalna achrasp...

journal_title：Journal of bacteriology

authors： SGUROS PL,SIMMS J

更新日期：1964-08-01 00:00:00

abstract：:Antibody in hyperimmune rabbit antisera specific for Staphylococcus aureus teichoic acid was shown to be associated with the IgM fraction. Treatment of such sera with mercaptoethanol destroyed its activity in passive mouse protection tests, whereas absorption with antirabbit IgG had no effect. Antibody response in nor...

journal_title：Journal of bacteriology

authors： Yoshida K,Ekstedt RD

更新日期：1968-11-01 00:00:00