Abstract:
:The filamentous cyanobacterium Anabaena sp. strain PCC 7120 can fix N(2) under oxic conditions, and the activity of nitrogen fixation occurs exclusively in heterocysts, cells differentiated from vegetative cells in response to a limitation of a combined-nitrogen source in the growth medium. At the late stages of heterocyst differentiation, an envelope layer composed of two glycolipids is formed to limit the entry of oxygen so that the oxygen-sensitive nitrogenase can function. The genome of Anabaena sp. strain PCC 7120 possesses a family of 13 genes (the hstK family), all encoding proteins with a putative Ser/Thr kinase domain at their N termini and a His-kinase domain at their C termini. In this study, we showed that the double mutant D4.3 strain, in which two members of this gene family, pkn44 (all1625) and pkn30 (all3691), were both inactivated, failed to fix N(2) in the presence of oxygen (Fox(-)). In an environment without oxygen, a low level of nitrogenase activity was detectable (Fix(+)). Heterocyst development in the mutant D4.3 was delayed by 24 h and arrested at a relatively early stage without the formation of the glycolipid layer (Hgl(-)). Only the minor species of the two heterocyst-specific glycolipids (HGLs) was missing in the mutant. We propose that DevH, a putative transcription factor, coordinates the synthesis of both HGLs, while Pkn44/Pkn30 and the previously characterized PrpJ may represent two distinct regulatory pathways involved in the synthesis of the minor HGL and the major HGL, respectively.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Shi L,Li JH,Cheng Y,Wang L,Chen WL,Zhang CCdoi
10.1128/JB.00323-07subject
Has Abstractpub_date
2007-07-01 00:00:00pages
5075-81issue
14eissn
0021-9193issn
1098-5530pii
JB.00323-07journal_volume
189pub_type
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