Abstract:
:A sequence-specific modification methylase (M . SinI) was isolated and purified from Escherichia coli harboring a derivative of recombinant plasmid pSI4 (see accompanying manuscript: C. Karreman and A. de Waard, J. Bacteriol. 170:2527-2532, 1988), which contains a Salmonella infantis DNA insert. The enzyme uniquely methylates the internal deoxycytidylate residue in the nucleotide sequence GG(A/T)MeCC, thereby protecting DNA completely against cleavage by restriction endonuclease R . SinI or R . AvaII [GG(A/T)CC], and in part against cleavage by R . Sau96I (GGNCC).
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Karreman C,de Waard Adoi
10.1128/jb.170.6.2533-2536.1988subject
Has Abstractpub_date
1988-06-01 00:00:00pages
2533-6issue
6eissn
0021-9193issn
1098-5530journal_volume
170pub_type
杂志文章abstract::The genetic context, phylogeny, and biochemistry of a gene flanking the H(2)-forming methylene-H(4)-methanopterin dehydrogenase gene (hmdA), here designated hmdB, indicate that it is a new member of the radical S-adenosylmethionine enzyme superfamily. In contrast to the characteristic CX(3)CX(2)C or CX(2)CX(4)C motif ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01125-09
更新日期:2010-01-01 00:00:00
abstract::Mutants of Bacillis subtilis that are temperature sensitive for RNA synthesis during sporulation were isolated after selection with a 32P suicide agent. Whole-genome sequencing revealed that two of the mutants carried an identical lesion in the rsbU gene, which encodes a phosphatase that indirectly activates SigB, the...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00212-17
更新日期:2017-06-27 00:00:00
abstract::A new Salmonella enterica serovar Typhimurium strain has been constructed to facilitate tightly regulated gene expression. Arabinose-inducible and glucose-repressible expression of a T7 RNA polymerase gene that has been integrated with an adjacent araC-P(BAD) control element into the bacterial chromosome allows dynami...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.21.6056-6059.2002
更新日期:2002-11-01 00:00:00
abstract::We previously described a gene, lpt3, required for the addition of phosphoethanolamine (PEtn) at the 3 position on the beta-chain heptose (HepII) of the inner-core Neisseria meningitidis lipopolysaccharide (LPS), but it has long been recognized that the inner-core LPS of some strains possesses PEtn at the 6 position (...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.20.6970-6982.2004
更新日期:2004-10-01 00:00:00
abstract::Previous investigations have demonstrated the existence of five Histoplasma capsulatum serotypes. Available specific fluorescent-antibody reagents stain only four of the five serotypes. Antibodies produced against the most complete H. capsulatum serotype were labeled with fluorescein isothiocyanate to develop a reagen...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.95.4.1243-1246.1968
更新日期:1968-04-01 00:00:00
abstract::The central metabolic model for Geobacter sulfurreducens included a single pathway for the biosynthesis of isoleucine that was analogous to that of Escherichia coli, in which the isoleucine precursor 2-oxobutanoate is generated from threonine. 13C labeling studies performed in G. sulfurreducens indicated that this pat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01841-07
更新日期:2008-04-01 00:00:00
abstract::Acetate kinase catalyzes the reversible phosphorylation of acetate (CH3COO- + ATP<-->CH3CO2PO3(2-) + ADP). A mechanism which involves a covalent phosphoryl-enzyme intermediate has been proposed, and chemical modification studies of the enzyme from Escherichia coli indicate an unspecified glutamate residue is phosphory...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.5.1129-1134.1998
更新日期:1998-03-01 00:00:00
abstract::An aryl aldehyde oxidoreductase from Nocardia sp. strain NRRL 5646 was purified 196-fold by a combination of Mono-Q, Reactive Green 19 agarose affinity, and hydroxyapatite chromatographies. The purified enzyme runs as a single band of 140 kDa on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The molecular ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.11.3482-3487.1997
更新日期:1997-06-01 00:00:00
abstract::Transcription-coupled repair (TCR) is a cellular process by which some forms of DNA damage are repaired more rapidly from transcribed strands of active genes than from nontranscribed strands or the overall genome. In humans, the TCR coupling factor, CSB, plays a critical role in restoring transcription following both ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.06725-11
更新日期:2012-05-01 00:00:00
abstract::The Bacillus anthracis endospore loses resistance properties during germination when its cortex peptidoglycan is degraded by germination-specific lytic enzymes (GSLEs). Although this event normally employs several GSLEs for complete cortex removal, the SleB protein alone can facilitate enough cortex hydrolysis to prod...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00869-10
更新日期:2011-01-01 00:00:00
abstract::The virD locus of Agrobacterium tumefaciens Ti plasmid pTiA6 was sequenced. Computer analysis of the sequence indicated five possible open reading frames (ORFs) within this locus. Two additional ORFs were identified distal to this locus. However, only two polypeptides of apparent molecular masses 16 and 56 kilodaltons...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.11.5035-5045.1987
更新日期:1987-11-01 00:00:00
abstract::Nocardia brasiliensis possess proteolytic activities that can be readily detected in a variety of media. In a modified formulation of a growth medium originally used for Streptomyces aureofaciens, N. brasiliensis was found to secrete proteolytic enzymes, one of which was capable of hydrolyzing casein. This enzyme was ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.157.2.627-631.1984
更新日期:1984-02-01 00:00:00
abstract::Recently we identified a bacterial factor (BimA) required for actin-based motility of Burkholderia pseudomallei. Here we report that Burkholderia mallei and Burkholderia thailandensis are capable of actin-based motility in J774.2 cells and that BimA homologs of these bacteria can restore the actin-based motility defec...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.22.7857-7862.2005
更新日期:2005-11-01 00:00:00
abstract::After heating at 65 C, ribosomes isolated from Bacillus stearothermophilus were strikingly more heat-stable than comparable preparations from Escherichia coli when tested for ability to support polyuridylic acid-directed phenylalanine incorporation at 37 C. The stability of ribosomes was also determined by measurement...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.93.5.1521-1526.1967
更新日期:1967-05-01 00:00:00
abstract::We are studying an O4/K54/H5 Escherichia coli bacteremic isolate (CP9) as a model pathogen for extraintestinal infection. Its group 2, K54 capsular polysaccharide is an important virulence determinant and confers serum resistance. In this study the effect of the group 1 capsule regulators, RcsA, RcsB, and Lon protease...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.23.7617-7623.1993
更新日期:1993-12-01 00:00:00
abstract::The rate of protein diffusion in bacterial cytoplasm may constrain a variety of cellular functions and limit the rates of many biochemical reactions in vivo. In this paper, we report noninvasive measurements of the apparent diffusion coefficient of green fluorescent protein (GFP) in the cytoplasm of Escherichia coli. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.1.197-203.1999
更新日期:1999-01-01 00:00:00
abstract::Screening of derivatives of Rhizobium etli KIM5s randomly mutagenized with mTn5SSgusA30 resulted in the identification of strain KIM-G1. Its rough colony appearance, flocculation in liquid culture, and Ndv(-) Fix(-) phenotype were indicative of a lipopolysaccharide (LPS) defect. Electrophoretic analysis of cell-associ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.18.5606-5614.1999
更新日期:1999-09-01 00:00:00
abstract::In both prokaryotes and eukaryotes, hydroxyurea is suggested to inhibit DNA replication by inactivating ribonucleotide reductase and depleting deoxyribonucleoside triphosphate pools. In this study, we show that the inhibition of replication in Escherichia coli is transient even at concentrations of 0.1 M hydroxyurea a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00713-17
更新日期:2018-02-23 00:00:00
abstract::A method for the transformation of Bacteroides fragilis with plasmid DNA was developed by using the clindamycin resistance plasmid pBFTM10 as the source of transforming DNA. The method was technically simple to perform and resulted in an average of 4.2 X 10(3) transformants per microgram of pBFTM10 added. A method for...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.164.1.466-469.1985
更新日期:1985-10-01 00:00:00
abstract::Expression of the Vibrio vulnificus metalloprotease gene, vvp, was turned up rapidly when bacterial growth reached the late log phase. A similar pattern of expression has been found in the metalloprotease gene of Vibrio cholerae, and this has been shown to be regulated by a Vibrio harveyi LuxR-like transcriptional act...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.4.1369-1375.2001
更新日期:2001-02-01 00:00:00
abstract::In Bacillus thuringiensis, a novel N-acetylmuramoyl-L-alanine amidase gene (named cwlB) was detected, and the CwlB protein was purified and characterized. Reverse transcription-PCR (RT-PCR) results indicated that cwlB and an upstream gene (named cwlA) formed one transcriptional unit. 5' rapid amplification of cDNA end...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00112-13
更新日期:2013-06-01 00:00:00
abstract::Cell walls of 19 Micromonospora species were analyzed for their components. All the cell walls had xylose and arabinose, but the presence of glucose, galactose, mannose, or rhamnose depended on the strain. Amino acids present in the walls consisted of glycine, glutamic acid, diaminopimelic acid, and alanine, in a mola...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.146.2.527-534.1981
更新日期:1981-05-01 00:00:00
abstract::Caldwell, D. R. (U.S. Department of Agriculture, Beltsville, Md.), D. C. White, M. P. Bryant, and R. N. Doetsch. Specificity of the heme requirement for growth of Bacteroides ruminicola. J. Bacteriol. 90:1645-1654. 1965.-Previous studies suggested that most strains of Bacteroides ruminicola subsp. ruminicola require h...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.90.6.1645-1654.1965
更新日期:1965-12-01 00:00:00
abstract::The AraC homolog ToxT coordinately regulates virulence gene expression in Vibrio cholerae. ToxT is required for transcriptional activation of the genes encoding cholera toxin and the toxin coregulated pilus, among others. In this work we focused on the interaction of ToxT with the tcpA promoter and investigated the me...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.20.5533-5544.2002
更新日期:2002-10-01 00:00:00
abstract::Growth of Agrobacterium tumefaciens on d-glucuronic acid (GlcUA) or d-galacturonic acid (GalUA) induces formation of hexuronic acid dehydrogenase [d-aldohexuronic acid: nicotinamide adenine dinucleotide (NAD) oxidoreductase]. The dehydrogenase, which irreversibly converts GlcUA or GalUA to the corresponding hexaric ac...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.99.3.667-673.1969
更新日期:1969-09-01 00:00:00
abstract::The type IV secretion system encoded by the virB operon is required for full virulence of Brucella sp., and the present study links the RNA chaperone Hfq to wild-type expression of virB in Brucella abortus 2308. Studies employing virB-lacZ fusions, quantitative reverse transcription-PCR, and immunoblot analysis showed...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.05623-11
更新日期:2012-01-01 00:00:00
abstract::Transcription of the tryptophanase (tna) operon of Escherichia coli is regulated by catabolite repression and tryptophan-induced transcription antitermination. Induction results from ribosome stalling after translation of tnaC, the coding region for a 24-residue leader peptide. The last sense codon of tnaC, proline co...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.188.5.1892-1898.2006
更新日期:2006-03-01 00:00:00
abstract::Whole-genome sequence analysis of Mycobacterium leprae has revealed a limited number of protein-coding genes, with half of the genome composed of pseudogenes and noncoding regions. We previously showed that some M. leprae pseudogenes are transcribed at high levels and that their expression levels change following infe...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00120-09
更新日期:2009-05-01 00:00:00
abstract::In Mexico, actinomycetoma is mainly caused by Nocardia brasiliensis, which is a soil inhabitant actinobacterium. Here, we report for the first time the draft genome of a strain isolated from a human case that has largely been found in in vitro and experimental models of actinomycetoma, N. brasiliensis HUJEG-1. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00210-12
更新日期:2012-05-01 00:00:00
abstract::The biochemical consequences of inositol limitation in an inositol auxotroph of Neurospora crassa have been examined as a means of disclosing the cellular role of inositol. The cellular levels of inositol in the inl mutant were proportional to the concentration of inositol in the growth medium whereas inositol phospha...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.143.1.18-26.1980
更新日期:1980-07-01 00:00:00