Functional characterization of a replication initiator protein.

abstract：:Exponentially growing populations of Bacillus subtilis contain two morphologically and functionally distinct cell types: motile individuals and nonmotile multicellular chains. Motility differentiation arises because RNA polymerase and the alternative sigma factor sigma(D) activate expression of flagellin in a subpopul...

journal_title：Journal of bacteriology

authors： Chen R,Guttenplan SB,Blair KM,Kearns DB

更新日期：2009-09-01 00:00:00

abstract：:A segment of Escherichia coli DNA that contained a discontinuity of homology with Salmonella typhimurium DNA was isolated. The segment, 1,430 base pairs long, was derived from one end of the lac "loop," a region of about 12 kilobase pairs of E. coli DNA, including the lac operon which has no detectable homology with S...

journal_title：Journal of bacteriology

authors： Buvinger WE,Lampel KA,Bojanowski RJ,Riley M

更新日期：1984-08-01 00:00:00

abstract：:The metD D-methionine transporter locus of Escherichia coli was identified as the abc-yaeE-yaeC cluster (now renamed metNIQ genes). The abc open reading frame is preceded by tandem MET boxes bracketed by the -10 and -35 boxes of a promoter. The expression driven by this promoter is controlled by the MetJ repressor and...

journal_title：Journal of bacteriology

authors： Gál J,Szvetnik A,Schnell R,Kálmán M

更新日期：2002-09-01 00:00:00

abstract：:A liquid chromatography-hybrid linear ion trap-Fourier transform ion cyclotron resonance mass spectrometry approach was used to determine the differential abundance of proteins in acetate-grown cells compared to that of proteins in methanol-grown cells of the marine isolate Methanosarcina acetivorans metabolically lab...

journal_title：Journal of bacteriology

authors： Li Q,Li L,Rejtar T,Lessner DJ,Karger BL,Ferry JG

更新日期：2006-01-01 00:00:00

abstract：:Wall turnover was studied in Bacillus subtilis. The loss of radioactively labeled wall polymers was followed during exponential growth in batch and chemostat cultures. Turnover kinetics were identical under all growth conditions; pulse-labeled wall material was lost with first-order kinetics, but only after exponentia...

journal_title：Journal of bacteriology

authors： De Boer WR,Kruyssen FJ,Wouters JT

更新日期：1981-01-01 00:00:00

abstract：:We isolated 28 mutants of Bacillus subtilis deficient in the development of competence by using the transposon Tn917lacZ as a mutagen. The mutant strains were poorly transformable with plasmid and chromosomal DNAs but were normally transducible and exhibited wild-type resistance to DNA-damaging agents. The mutations w...

journal_title：Journal of bacteriology

authors： Hahn J,Albano M,Dubnau D

更新日期：1987-07-01 00:00:00

abstract：:Cells of the gliding bacterium Flavobacterium johnsoniae move rapidly over surfaces. Mutations in gldN cause a partial defect in gliding. A novel bacteriophage selection strategy was used to aid construction of a strain with a deletion spanning gldN and the closely related gene gldO in an otherwise wild-type F. johnso...

journal_title：Journal of bacteriology

authors： Rhodes RG,Samarasam MN,Shrivastava A,van Baaren JM,Pochiraju S,Bollampalli S,McBride MJ

更新日期：2010-03-01 00:00:00

abstract：:Escherichia coli AT2046 has been shown previously to lack the enzyme serine transhydroxymethylase and to require exogenous glycine for growth as a consequence. Strains JEV73 and JEV73R, mutants derived from strain AT2046, are shown here to be serine transhydroxymethylase deficient, but able to derive their glycine fro...

journal_title：Journal of bacteriology

authors： Fraser J,Newman EB

更新日期：1975-06-01 00:00:00

abstract：:The deacetylcephalosporin C synthetase (hydroxylase) gene from Streptomyces clavuligerus has been cloned and sequenced. The open reading frame codes for a protein with an Mr of 34,584. The hydroxylase gene (cefF) is closely linked to the epimerase gene (cefD) and the expandase gene (cefE) and is transcribed in the opp...

journal_title：Journal of bacteriology

authors： Kovacevic S,Miller JR

更新日期：1991-01-01 00:00:00

abstract：:Generally nonchemotactic mutants of Escherichia coli and Salmonella typhimurium were analyzed by interspecies complementation tests to determine the functional correspondence between the che genes of these two organisms. The E. coli che region was introduced into Salmonella recipients by means of a series of F-prime e...

journal_title：Journal of bacteriology

authors： DeFranco AL,Parkinson JS,Koshland DE Jr

更新日期：1979-07-01 00:00:00

abstract：:Type IIb heat-labile enterotoxin (LT-IIb) is produced by Escherichia coli 41. Restriction fragments of total cell DNA from strain 41 were cloned into a cosmid vector, and one cosmid clone that encoded LT-IIb was identified. The genes for LT-IIb were subcloned into a variety of plasmids, expressed in minicells, sequenc...

journal_title：Journal of bacteriology

authors： Pickett CL,Twiddy EM,Coker C,Holmes RK

更新日期：1989-09-01 00:00:00

abstract：:Type III machines of pathogenic Yersinia spp. transport Yop proteins across the bacterial envelope into host cells. Translational fusions of yopE to the dihydrofolate reductase gene (dhfr) or the beta-galactosidase gene (lacZ) generate hybrid proteins that block type III injection of Yop proteins into host cells, cons...

journal_title：Journal of bacteriology

authors： Sorg JA,Miller NC,Marketon MM,Schneewind O

更新日期：2005-10-01 00:00:00

abstract：:We report the draft genome sequence of methicillin-resistant Staphylococcus aureus (MRSA) strain ST672, an emerging disease clone in India, from a septicemia patient. The genome size is about 2.82 Mb with 2,485 open reading frames (ORFs). The staphylococcal cassette chromosome mec (SCCmec) element (type V) and immune ...

journal_title：Journal of bacteriology

authors： Khedkar S,Prabhakara S,Loganathan RM,S C,Gowda M,Arakere G,Seshasayee AS

更新日期：2012-12-01 00:00:00

abstract：:Stalk formation is a novel pattern of multicellular organization. Yeast cells which survive UV irradiation form colonies that grow vertically to form very long (0.5 to 3.0 cm) and thin (0.5 to 4 mm in diameter) multicellular structures. We describe the conditions required to obtain these stalk-like structures reproduc...

journal_title：Journal of bacteriology

authors： Engelberg D,Mimran A,Martinetto H,Otto J,Simchen G,Karin M,Fink GR

更新日期：1998-08-01 00:00:00

abstract：:Relatedness in species of Erwinia was assessed by determining the extent of reassociation in heterologous deoxyribonucleic acid preparations. Thermal elution chromatography on hydroxyapatite was used to separate reassociated nucleotide sequences from nonreassociated sequences and to determine the thermal stability of ...

journal_title：Journal of bacteriology

authors： Brenner DJ,Fanning GR,Steigerwalt AG

更新日期：1972-04-01 00:00:00

abstract：:DNA sequence analysis of a region of the Streptomyces sp. strain C5 daunomycin biosynthesis gene cluster, located between the daunomycin polyketide biosynthesis gene cluster and a dnrI (transcriptional activator) homolog, revealed the presence of a gene encoding a P-450-like enzyme with a deduced Mr of 46,096. Express...

journal_title：Journal of bacteriology

authors： Dickens ML,Strohl WR

更新日期：1996-06-01 00:00:00

abstract：:Pseudomonas sp. strain LB400 grows on biphenyl as the sole carbon and energy source. This organism also cooxidizes several chlorinated biphenyl congeners. Biphenyl dioxygenase activity in cell extract required addition of NAD(P)H as an electron donor for the conversion of biphenyl to cis-2,3-dihydroxy-2,3-dihydrobiphe...

journal_title：Journal of bacteriology

authors： Haddock JD,Nadim LM,Gibson DT

更新日期：1993-01-01 00:00:00

abstract：:The antiadaptor protein IraD inhibits the proteolysis of the alternative sigma factor, RpoS, which promotes the synthesis of >100 genes during the general stress response and during stationary phase. Our previous results showed that IraD determines RpoS steady-state levels during exponential growth and mediates its st...

journal_title：Journal of bacteriology

authors： Merrikh H,Ferrazzoli AE,Lovett ST

更新日期：2009-12-01 00:00:00

abstract：:The tar and tap loci of Escherichia coli encode methyl-accepting inner membrane proteins that mediate chemotactic responses to aspartate and maltose or to dipeptides. These genes lie adjacent to each other in the same orientation on the chromosome and have extensive sequence homology throughout the C-terminal portions...

journal_title：Journal of bacteriology

authors： Slocum MK,Halden NF,Parkinson JS

更新日期：1987-07-01 00:00:00

abstract：:The lipoteichoic acids were isolated from phenol extracts of four Listeria strains representing serotypes 4a, 4b, 6a, and 6 to compare the differences in structure of amphiphilic polysaccharides from various serotypes of Listeria spp. The lipoteichoic acids from the four strains examined had the same structure in both...

journal_title：Journal of bacteriology

authors： Uchikawa K,Sekikawa I,Azuma I

更新日期：1986-10-01 00:00:00

abstract：:A strain of Veillonella parvula M4, which grows readily in lactate broth without a requirement for carbon dioxide, has been isolated from the oral cavity. Anaerobic, washed cells of this organism fermented sodium lactate to the following products (moles/100 moles of lactate): propionate, 66; acetate, 40; carbon dioxid...

journal_title：Journal of bacteriology

authors： Ng SK,Hamilton IR

更新日期：1971-03-01 00:00:00

abstract：:Expression of the Bacillus subtilis trpEDCFBA operon has been shown to be regulated by transcription attenuation in response to the availability of L-tryptophan. Regulation is mediated by the tryptophan-activated trp RNA-binding attenuation protein, TRAP, the product of mtrB. Formation of mutually exclusive RNA anti-t...

journal_title：Journal of bacteriology

authors： Merino E,Babitzke P,Yanofsky C

更新日期：1995-11-01 00:00:00

abstract：:A total of 12 VanA-type vancomycin-resistant enterococci, consisting of 10 Enterococcus faecium isolates and two Enterococcus avium isolates, were examined in detail. The vancomycin resistance conjugative plasmids pHTalpha (65.9 kbp), pHTbeta (63.7 kbp), and pHTgamma (66.5 kbp) were isolated from each of three differe...

journal_title：Journal of bacteriology

authors： Tomita H,Tanimoto K,Hayakawa S,Morinaga K,Ezaki K,Oshima H,Ike Y

更新日期：2003-12-01 00:00:00

abstract：:The gene for staphylococcal enterotoxin type E (entE) was cloned from Staphylococcus aureus into plasmid vector pBR322 and introduced into Escherichia coli. A staphylococcal enterotoxin type E-producing E. coli strain was isolated. The complete nucleotide sequence of the cloned structural entE gene and the N-terminal ...

journal_title：Journal of bacteriology

authors： Couch JL,Soltis MT,Betley MJ

更新日期：1988-07-01 00:00:00

abstract：:Sbarra, Anthony J. (St. Margaret's Hospital, Boston, Mass.), William Shirley, and John S. Baumstark. Effect of osmolarity on phagocytosis. J. Bacteriol. 85:306-313. 1963.-The effect of a number of different compounds on phagocytosis was studied. Phagocytosis was monitored by morphological and biochemical means. It was...

journal_title：Journal of bacteriology

authors： SBARRA AJ,SHIRLEY W,BAUMSTARK JS

更新日期：1963-02-01 00:00:00

abstract：:Procedures for the purification of catechol 1,2-dioxygenase from extracts of Acinetobacter calcoaceticus strain ADP-96 are described. The purified enzyme was homogeneous as judged by ultracentrifugation and acrylamide gel electrophoresis. The enzyme contained 2 g-atoms of iron per mol of protein. The enzyme had a broa...

journal_title：Journal of bacteriology

authors： Patel RN,Hou CT,Felix A,Lillard MO

更新日期：1976-07-01 00:00:00

abstract：:An extracellular, 700,000-Mr multiprotein complex that catalyzed the hydrolysis of crystalline cellulose (Avicel) was isolated from cultures of Clostridium sp. strain C7, a mesophile from freshwater sediment. In addition to cellulose (Avicel, ball-milled filter paper), the multiprotein complex hydrolyzed carboxymethyl...

journal_title：Journal of bacteriology

authors： Cavedon K,Leschine SB,Canale-Parola E

更新日期：1990-08-01 00:00:00

abstract：:The papA gene of the uropathogenic strain Escherichia coli J96, coding for the Pap pili subunit, was subjected to DNA sequencing, and found to code for an 185-amino acid-long polypeptide with a 22-amino acid-long signal peptide. Here we present the primary sequence, the hydrophilicity profile, and the predicted polype...

journal_title：Journal of bacteriology

authors： Båga M,Normark S,Hardy J,O'Hanley P,Lark D,Olsson O,Schoolnik G,Falkow S

更新日期：1984-01-01 00:00:00

abstract：:Streptococcus pyogenes (group A streptococcus [GAS]), a catalase-negative gram-positive bacterium, is aerotolerant and survives H2O2 exposures that kill many catalase-positive bacteria. The molecular basis of the H2O2 resistance is poorly known. Here, we demonstrate that serotype M49 GAS lacking the Rgg regulator is m...

journal_title：Journal of bacteriology

authors： Pulliainen AT,Hytönen J,Haataja S,Finne J

更新日期：2008-05-01 00:00:00

abstract：:Girard, Kenneth F. (St. Margaret's Hospital, Boston, Mass.), Anthony J. Sbarra, and Wadi A. Bardawil. Serology of Listeria monocytogenes. I. Characteristics of the soluble hemolysin. J. Bacteriol. 85:349-355. 1963.-Our results clearly demonstrate that Listeria monocytogenes (strain 9-125) produces a soluble hemolysin....

journal_title：Journal of bacteriology

authors： GIRARD KF,SBARRA AJ,BARDAWIL WA

更新日期：1963-02-01 00:00:00