Preferential selection of deletion mutations of the outer membrane lipoprotein gene of Escherichia coli by globomycin.

abstract：:We have sequenced the entire region of DNA required for the biosynthesis of CS5 pili from enterotoxigenic Escherichia coli O115:H40 downstream of the major subunit gene, designated csfA (for coli surface factor five A). Five more open reading frames (ORFs) (csfB, csfC, csfE, csfF, and csfD) which are transcribed in th...

journal_title：Journal of bacteriology

authors： Duthy TG,Staendner LH,Manning PA,Heuzenroeder MW

更新日期：1999-09-01 00:00:00

abstract：:The abundance of oxidants and reductants must be balanced for an organism to thrive. Bacteria have evolved methods to prevent redox imbalances and to mitigate their deleterious consequences through the expression of detoxification enzymes, antioxidants, and systems to repair or degrade damaged proteins and DNA. Regula...

journal_title：Journal of bacteriology

authors： Reniere ML

更新日期：2018-08-10 00:00:00

abstract：:The serotype 5 capsule gene cluster of Staphylococcus aureus comprises 16 genes (cap5A through cap5P), but little is known about how the putative gene products function in capsule biosynthesis. We propose that the N-acetylmannosaminuronic acid (ManNAcA) component of the S. aureus serotype 5 capsular polysaccharide (CP...

journal_title：Journal of bacteriology

authors： Kiser KB,Bhasin N,Deng L,Lee JC

更新日期：1999-08-01 00:00:00

abstract：:The SasG surface protein of Staphylococcus aureus has been shown to promote the formation of biofilm. SasG comprises an N-terminal A domain and repeated B domains. Here we demonstrate that SasG is involved in the accumulation phase of biofilm, a process that requires a physiological concentration of Zn(2+). The B doma...

journal_title：Journal of bacteriology

authors： Geoghegan JA,Corrigan RM,Gruszka DT,Speziale P,O'Gara JP,Potts JR,Foster TJ

更新日期：2010-11-01 00:00:00

abstract：:Sequences essential to the chloramphenicol-inducible expression of cat-86, a chloramphenicol acetyltransferase gene, reside in a 144-base pair (bp) regulatory region that intervenes between the cat-86 coding sequence and its promoter. A key regulatory element within the 144-bp segment consists of a pair of inverted-re...

journal_title：Journal of bacteriology

authors： Ambulos NP Jr,Mongkolsuk S,Kaufman JD,Lovett PS

更新日期：1985-11-01 00:00:00

abstract：:The expression of any given bacterial protein is predicted to depend on (i) the transcriptional regulation of the promoter and the translational regulation of its mRNA and (ii) the synthesis and translation of total (bulk) mRNA. This is because total mRNA acts as a competitor to the specific mRNA for the binding of in...

journal_title：Journal of bacteriology

authors： Liang ST,Xu YC,Dennis P,Bremer H

更新日期：2000-06-01 00:00:00

abstract：:The gene encoding an extracellular isomalto-dextranase, designated imd, was isolated from the chromosomal DNA of Arthrobacter globiformis T6 and cloned and expressed in Escherichia coli. A single open reading frame consisting of 1,926 base pairs that encoded a polypeptide composed of a signal peptide of 39 amino acids...

journal_title：Journal of bacteriology

authors： Iwai A,Ito H,Mizuno T,Mori H,Matsui H,Honma M,Okada G,Chiba S

更新日期：1994-12-01 00:00:00

abstract：:Streptococcus bovis JB1 cells energized with glucose transported glutamine at a rate of 7 nmol/mg of protein per min at a pH of 5.0 to 7.5; sodium had little effect on the transport rate. Because valinomycin-treated cells loaded with K and diluted into Na (pH 6.5) to create an artificial delta psi took up little gluta...

journal_title：Journal of bacteriology

authors： Chen GJ,Russell JB

更新日期：1989-06-01 00:00:00

abstract：:A proline analogue, 4,5-dehydro-l-pipecolic acid (baikiain) induces the formation in Salmonella typhimurium of the two enzymes catalyzing the degradation of proline, proline oxidase and Delta(1)-pyrroline-5-carboxylic acid (P5C) dehydrogenase. The level of induction by 20 mm baikiain is about 10% of the maximum level ...

journal_title：Journal of bacteriology

authors： Dendinger S,Brill WJ

更新日期：1972-12-01 00:00:00

abstract：:SecA signal peptide interaction is critical for initiating protein translocation in the bacterial Sec-dependent pathway. Here, we have utilized the recent nuclear magnetic resonance (NMR) and Förster resonance energy transfer studies that mapped the location of the SecA signal peptide-binding site to design and isolat...

journal_title：Journal of bacteriology

authors： Grady LM,Michtavy J,Oliver DB

更新日期：2012-01-01 00:00:00

abstract：:In order to predict the binding regions within the complex formed by Toll-like receptor 5 (TLR-5) and flagellin, a complementary hydropathy between the two proteins was sought. A region common to the flagellins of Salmonella enterica serovar Typhimurium, Pseudomonas aeruginosa, and Listeria monocytogenes was shown to ...

journal_title：Journal of bacteriology

authors： Jacchieri SG,Torquato R,Brentani RR

更新日期：2003-07-01 00:00:00

abstract：:Structural analogs of S-adenosylhomocysteine were tested in vitro for inhibition of the yeast S-adenosylmethionine:delta 24-sterol-C-methyltransferase enzyme. A wide inhibitory range by these compounds was observed, suggesting which structural features of the parent compound are important for binding to the enzyme. No...

journal_title：Journal of bacteriology

authors： McCammon MT,Parks LW

更新日期：1981-01-01 00:00:00

abstract：:Correct targeting of membrane proteins is essential for membrane integrity, cell physiology, and viability. Cotranslational targeting depends on the universally conserved signal recognition particle (SRP), which is a ribonucleoprotein complex comprised of the protein component Ffh and the 4.5S RNA in Escherichia coli ...

journal_title：Journal of bacteriology

authors： Sauerbrei B,Arends J,Schünemann D,Narberhaus F

更新日期：2020-06-25 00:00:00

abstract：:Resistance of Staphylococcus aureus mediated by the penicillinase (Pc-ase) plasmid to divalent metal ions of Hg and Cd was found to be controlled by different mechanisms. The Hg resistance of the Pc-ase plasmid-carrying organisms is based upon a process of changing the ion incorporated in the cell into a somewhat inno...

journal_title：Journal of bacteriology

authors： Kondo I,Ishikawa T,Nakahara H

更新日期：1974-01-01 00:00:00

abstract：:5-Azacytidine was found to be bactericidal to Escherichia coli carrying plasmids specifying EcoRII restriction-modification systems, but not to the same strains lacking these plasmids. Of other base analogs tested, only 5(beta-D-ribofuranosyl)isocytidine had similar, although weaker, effects. Plasmids that had lost th...

journal_title：Journal of bacteriology

authors： Friedman S

更新日期：1982-07-01 00:00:00

abstract：:Using physical and genetic data, we have demonstrated that Rhizobium meliloti SU47 has a symbiotic megaplasmid, pRmeSU47b, in addition to the previously described nod-nif megaplasmid pRmeSU47a. This plasmid includes four loci involved in exopolysaccharide (exo) synthesis as well as two loci involved in thiamine biosyn...

journal_title：Journal of bacteriology

authors： Finan TM,Kunkel B,De Vos GF,Signer ER

更新日期：1986-07-01 00:00:00

abstract：:Three Escherichia coli phages, TuIa, TuIb, and TuII, were isolated from local sewage. We present evidence that they use the major outer membrane proteins Ia, Ib, and II, respectively, as receptors. In all cases the proteins, under the experimental conditions used, required lipopolysaccharide to exhibit their receptor ...

journal_title：Journal of bacteriology

authors： Datta DB,Arden B,Henning U

更新日期：1977-09-01 00:00:00

abstract：:Analysis of the genome sequence of Enterococcus faecalis allowed the identification of two genes whose protein products showed 33 and 34% identity with those of sigV and yrhM of Bacillus subtilis, respectively. These genes, named sigV and rsiV, are predicted to encode members of the extracytoplasmic function subfamily...

journal_title：Journal of bacteriology

authors： Benachour A,Muller C,Dabrowski-Coton M,Le Breton Y,Giard JC,Rincé A,Auffray Y,Hartke A

更新日期：2005-02-01 00:00:00

abstract：:The gltA gene, encoding Sinorhizobium meliloti 104A14 citrate synthase, was isolated by complementing an Escherichia coli gltA mutant. The S. meliloti gltA gene was mutated by inserting a kanamycin resistance gene and then using homologous recombination to replace the wild-type gltA with the gltA::kan allele. The resu...

journal_title：Journal of bacteriology

authors： Mortimer MW,McDermott TR,York GM,Walker GC,Kahn ML

更新日期：1999-12-01 00:00:00

abstract：:Application of in vivo expression technology (IVET) to Yersinia ruckeri, an important fish pathogen, allowed the identification of two adjacent genes that represent a novel bacterial system involved in the uptake and degradation of l-cysteine. Analysis of the translational products of both genes showed permease domain...

journal_title：Journal of bacteriology

authors： Méndez J,Reimundo P,Pérez-Pascual D,Navais R,Gómez E,Guijarro JA

更新日期：2011-02-01 00:00:00

abstract：:Adjacent intein fragments fused to a Snf2/Rad54 helicase-related protein and Snf2/Rad54 helicase were reported for Deinococcus radiodurans R1, leading to the speculation that a frameshift was required for splicing or that trans splicing occurred. However, a type strain (ATCC 13939, RF18410) yielded a single protein th...

journal_title：Journal of bacteriology

authors： Southworth MW,Perler FB

更新日期：2002-11-01 00:00:00

abstract：:DNA microarrays encompassing the entire genome of Yersinia pestis were used to characterize global regulatory changes during steady-state vegetative growth occurring after shift from 26 to 37 degrees C in the presence and absence of Ca2+. Transcriptional profiles revealed that 51, 4, and 13 respective genes and open r...

journal_title：Journal of bacteriology

authors： Motin VL,Georgescu AM,Fitch JP,Gu PP,Nelson DO,Mabery SL,Garnham JB,Sokhansanj BA,Ott LL,Coleman MA,Elliott JM,Kegelmeyer LM,Wyrobek AJ,Slezak TR,Brubaker RR,Garcia E

更新日期：2004-09-01 00:00:00

abstract：:Both the acridine half-mustard, ICR191, and the nonalkylating azaacridine derivative, ICR364-OH, induce three classes of frameshift mutations in the histidine operon of Salmonella typhimurium. (i) One class is completely stable in reversion tests and is presumed to represent deletion of one or a few critical nucleotid...

journal_title：Journal of bacteriology

authors： Oeschger NS,Hartman PE

更新日期：1970-02-01 00:00:00

abstract：:A library of Zymomonas mobilis genomic DNA was constructed in the broad-host-range cosmid pLAFR1. The library was mobilized into a variety of Escherichia coli and Pseudomonas putida trp mutants by using the helper plasmid pRK2013. Five Z. mobilis trp genes were identified by the ability to complement the trp mutants. ...

journal_title：Journal of bacteriology

authors： Eddy CK,Smith OH,Noel KD

更新日期：1988-07-01 00:00:00

abstract：:A total of 508 random clones from five Mycoplasma genitalium genomic libraries were partially sequenced and analyzed. This resulted in the identification of 291 unique contigs. Sequence information from these clones (100,993 nucleotides), representing approximately 17% of this pathogen's genome, was analyzed by compar...

journal_title：Journal of bacteriology

authors： Peterson SN,Hu PC,Bott KF,Hutchison CA 3rd

更新日期：1993-12-01 00:00:00

abstract：:Escherichia coli MutY and MutS increase replication fidelity by removing adenines that were misincorporated opposite 7,8-dihydro-8-oxo-deoxyguanines (8-oxoG), G, or C. MutY DNA glycosylase removes adenines from these mismatches through a short-patch base excision repair pathway and thus prevents G:C-to-T:A and A:T-to-...

journal_title：Journal of bacteriology

authors： Bai H,Lu AL

更新日期：2007-02-01 00:00:00

abstract：:Langridge, Patricia (Oregon State University, Corvallis), and Richard Y. Morita. Thermolability of malic dehydrogenase from the obligate psychrophile Vibrio marinus. J. Bacteriol. 92:418-423. 1966.-The thermolability of malic dehydrogenase in whole cells of Vibrio marinus MP-1 grown at 15 C was compared with that of c...

journal_title：Journal of bacteriology

authors： Langridge P,Morita RY

更新日期：1966-08-01 00:00:00

abstract：:Cupric sulfate induced mRNA specific to the copper resistance gene cluster previously cloned from Pseudomonas syringae pv. tomato PT23. mRNA from each of the four genes of this cluster responded in a similar manner to induction over time and with different concentrations of cupric sulfate. Promoter fusion constructs i...

journal_title：Journal of bacteriology

authors： Mellano MA,Cooksey DA

更新日期：1988-09-01 00:00:00

abstract：:The disaccharide trehalose is accumulated as a storage product by spores of Streptomyces griseus. Nongerminating spores used their trehalose reserves slowly when incubated in buffer for several months. In contrast, spores rapidly depleted their trehalose pools during the first hours of germination. Extracts of dormant...

journal_title：Journal of bacteriology

authors： McBride MJ,Ensign JC

更新日期：1987-11-01 00:00:00

abstract：:Whole-genome sequence analysis of Mycobacterium leprae has revealed a limited number of protein-coding genes, with half of the genome composed of pseudogenes and noncoding regions. We previously showed that some M. leprae pseudogenes are transcribed at high levels and that their expression levels change following infe...

journal_title：Journal of bacteriology

authors： Akama T,Suzuki K,Tanigawa K,Kawashima A,Wu H,Nakata N,Osana Y,Sakakibara Y,Ishii N

更新日期：2009-05-01 00:00:00