Abstract:
:Azotobacter vinelandii is a bacterium which undergoes a differentiation process leading to the formation of metabolically dormant cysts. During the encystment process, A. vinelandii produces alkylresorcinol lipids (ARs) that replace the membrane phospholipids and are also components of the layers covering the cyst. The synthesis of ARs in A. vinelandii has been shown to occur by the activity of enzymes encoded by the arsABCD operon, which is expressed only during the differentiation process. Also, the production of ARs has been shown to be dependent on the stationary-phase sigma factor RpoS, which is also implicated in the control of the synthesis of other cyst components (i.e., alginate and poly-β-hydroxybutyrate). In this study, we identified ArpR, a LysR-type transcriptional regulator expressed only during encystment that positively regulates arsABCD transcription. We show that this activation is dependent on acetoacetyl-coenzyme A (acetoacetyl-CoA), which might provide a metabolic signal for encystment. We also show that RpoS regulates arsABCD expression through the control of arpR transcription.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Romero Y,Moreno S,Guzmán J,Espín G,Segura Ddoi
10.1128/JB.01946-12subject
Has Abstractpub_date
2013-04-01 00:00:00pages
1834-44issue
8eissn
0021-9193issn
1098-5530pii
JB.01946-12journal_volume
195pub_type
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更新日期:1982-07-01 00:00:00
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更新日期:2003-12-01 00:00:00