Abstract:
:We report here the structure of a previously uncharacterized phospholipid in the radiation-resistant bacterium Deinococcus radiodurans. This phospholipid, designated lipid 4, was shown by chemical analysis, HF hydrolysis, and nuclear magnetic resonance spectroscopy to be phosphatidylglyceroylalkylamine. Lipid 4 thus contains the unusual lipid constituents glyceric acid and alkylamines, which have previously been identified in two complex phosphoglycolipids from this organism. By [32P]phosphate pulse-chase labeling techniques, lipid 4 was shown to be the precursor of the complex phosphoglycolipids alpha-galactosyl- and alpha-N-acetylglucosaminylphosphatidylglyceroylalkylamine. While phosphatidylglyceroylalkylamine is rapidly biosynthesized from Pi, its subsequent glycosylation occurs much more slowly. Therefore, we conclude that the final glycosylation step is the rate-limiting event in the biosynthesis of the complex phosphoglycolipids alpha-galactosyl- and alpha-N-acetylglucosaminyl-phosphatidylglyceroylalkylamine.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Huang Y,Anderson Rdoi
10.1128/jb.173.2.457-462.1991subject
Has Abstractpub_date
1991-01-01 00:00:00pages
457-62issue
2eissn
0021-9193issn
1098-5530journal_volume
173pub_type
杂志文章abstract::We have identified recD mutants of Salmonella typhimurium by their ability to support growth of phage P22 abc (anti-RecBCD) mutants, whose growth is prevented by normal host RecBCD function. As in Escherichia coli, the recD gene of S. typhimurium lies between the recB and argA genes at min 61 of the genetic map. Plasm...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.13.4092-4103.1994
更新日期:1994-07-01 00:00:00
abstract::The main chain of teichoic acids can be assembled in cell-free membrane preparations by the transfer of residues from the appropriate nucleotide precursors to an incompletely characterized amphiphilic molecule, lipoteichoic acid carrier (LTC). However, in the cell wall, the main chain is attached to peptidoglycan thro...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.145.3.1222-1231.1981
更新日期:1981-03-01 00:00:00
abstract::Strain DCB-1 is a strict anaerobe capable of reductive dehalogenation. We elucidated metabolic processes in DCB-1 which may be related to dehalogenation and which further characterize the organism physiologically. Sulfoxy anions and CO2 were used by DCB-1 as catabolic electron acceptors. With suitable electron donors,...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.4.2065-2070.1990
更新日期:1990-04-01 00:00:00
abstract::The tprK gene of Treponema pallidum subsp. pallidum, the causative agent of venereal syphilis, belongs to a 12-member gene family and encodes a protein with a predicted cleavable signal sequence and predicted transmembrane domains. Except for the Nichols type strain, all rabbit-propagated isolates of T. pallidum exami...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.21.6262-6268.2003
更新日期:2003-11-01 00:00:00
abstract::Extracytoplasmic function σ factors that are stress inducible are often sequestered in an inactive complex with a membrane-associated anti-σ factor. Mycobacterium tuberculosis membrane-associated anti-σ factors have a small, stable RNA gene A (ssrA)-like degron for targeted proteolysis. Interaction between the unfolda...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00748-18
更新日期:2019-02-25 00:00:00
abstract::Besides producing thymine-requiring mutants (thy), trimethoprim (TMP) cured the mini-ColE1 replicon pML21 at an appreciable frequency. The cured Escherichia coli K-12 cells behaved like polA mutants by failing to support the stable maintenance of the ColE1 plasmid. The mini-F replicon pSC138, which was lacking all thr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.150.2.755-761.1982
更新日期:1982-05-01 00:00:00
abstract::Bacteroides thetaiotaomicron, a gram-negative obligate anaerobe, appears to utilize starch by first binding the polymer to its surface and then translocating it into the periplasmic space. Several genes that encode enzymes or outer membrane proteins involved in starch utilization have been identified. These have been ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.24.7180-7186.1996
更新日期:1996-12-01 00:00:00
abstract::Analysis of the genome sequence of Enterococcus faecalis allowed the identification of two genes whose protein products showed 33 and 34% identity with those of sigV and yrhM of Bacillus subtilis, respectively. These genes, named sigV and rsiV, are predicted to encode members of the extracytoplasmic function subfamily...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.3.1022-1035.2005
更新日期:2005-02-01 00:00:00
abstract::Acinetobacter sp. strain JC1 DSM 3803, a carboxydobacterium, grown on methanol was found to show dihydroxyacetone synthase, dihydroxyacetone kinase, and ribulose 1,5-bisphosphate carboxylase, but no hydroxypyruvate reductase and very low hexulose 6-phosphate synthase, activities. The dihydroxyacetone synthase was foun...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.19.6041-6047.1997
更新日期:1997-10-01 00:00:00
abstract::Uranyl nitrate inhibited the transport of several amino acids, a vitamin, and a disaccharide into yeast. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.105.3.1205-1206.1971
更新日期:1971-03-01 00:00:00
abstract::Bacteriocin JF246 has been purified from mitomycin C-induced Serratia marcescens cells by salt extraction, ammonium sulfate fractionation, and chromatography on QAE-Sephadex and SP-Sephadex. The purified material is homogeneous on polyacrylamide gel electrophoresis in the presence of 2% sodium dodecyl sulfate or 6 m u...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.110.3.1001-1009.1972
更新日期:1972-06-01 00:00:00
abstract::The CbpA protein is an analog of the DnaJ molecular chaperone of Escherichia coli. To gain insight into the function of CbpA, we examined the nature of a cbpA null mutation with special reference to those of dnaK and dnaJ null mutations. In particular, the cbpA dnaJ double-null mutant was found to exhibit severe defec...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.13.3894-3896.1995
更新日期:1995-07-01 00:00:00
abstract::In gammaproteobacteria the Hfq protein shows a great variation in size, especially in its C-terminal part. Extremely large Hfq proteins consisting of almost 200 amino acid residues and more are found within the gammaproteobacterial family Moraxellaceae. The difference in size compared to other Hfq proteins is due to a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00490-09
更新日期:2009-09-01 00:00:00
abstract::The type III secretion (T3S) system is essential to the virulence of a large number of Gram-negative bacterial pathogens, including Yersinia. YscO is required for T3S in Yersinia and is known to interact with several other T3S proteins, including the chaperone SycD and the needle length regulator YscP. To define which...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00876-13
更新日期:2013-10-01 00:00:00
abstract::Bacteria within the Mycobacterium avium complex are prominent in the environment and are a source of serious disseminated infections in patients with AIDS. Serovars of the M. avium complex are distinguished from all other mycobacteria and from one another by the presence of highly antigenic glycolipids, the glycopepti...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.21.6991-6997.1991
更新日期:1991-11-01 00:00:00
abstract::The group A streptococcus (GAS), Streptococcus pyogenes, is an important human pathogen that causes infections ranging in severity from self-limiting pharyngitis to severe invasive diseases that are associated with significant morbidity and mortality. The pathogenic effects of GAS are mediated by the expression of vir...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00198-06
更新日期:2006-07-01 00:00:00
abstract::Escherichia coli has been used as a platform host for studying the production of free fatty acids (FFA) and other energy-dense compounds useful in biofuel applications. Most of the FFA produced by E. coli are found extracellularly. This finding suggests that a mechanism for transport across the cell envelope exists, y...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01477-12
更新日期:2013-01-01 00:00:00
abstract::Heating competent Azotobacter vinelandii at 37 or 42 degrees C resulted in a total loss of competence with no loss of viability. The transformation process was relatively insensitive to heating at either temperature once DNase-resistant DNA binding was nearly complete. Although competent and 42 degrees C-treated cells...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.155.1.159-168.1983
更新日期:1983-07-01 00:00:00
abstract::The uhp locus of Escherichia coli contains genes for the sugar phosphate transport system (uhpT) and the regulatory system which allows its induction by external glucose 6-phosphate (uhpRA). The uhp region was cloned onto high-copy-number plasmids, both from Uhp(+) plasmids of the Clarke-Carbon collection and from gen...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.155.3.1062-1070.1983
更新日期:1983-09-01 00:00:00
abstract::Thiocyanate hydrolase is a newly found enzyme from Thiobacillus thioparus THI 115 that converts thiocyanate to carbonyl sulfide and ammonia (Y. Katayama, Y. Narahara, Y. Inoue, F. Amano, T. Kanagawa, and H. Kuraishi, J. Biol. Chem. 267:9170-9175, 1992). We have cloned and sequenced the scn genes that encode the three ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.10.2583-2589.1998
更新日期:1998-05-01 00:00:00
abstract::Nitrogenase activity in Rhodospirillum rubrum and in some other photosynthetic bacteria is regulated in part by the availability of light. This regulation is through a posttranslational modification system that is itself regulated by P(II) homologs in the cell. P(II) is one of the most broadly distributed regulatory p...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00585-09
更新日期:2009-09-01 00:00:00
abstract::The DNA binding proteins ArgR and AhrC are essential for regulation of arginine metabolism in Escherichia coli and Bacillus subtilis, respectively. A unique property of these regulators is that they form hexameric protein complexes, mediating repression of arginine biosynthetic pathways as well as activation of argini...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.186.4.1147-1157.2004
更新日期:2004-02-01 00:00:00
abstract::The Tol-Pal system of Escherichia coli is involved in maintaining outer membrane stability. Mutations in tolQ, tolR, tolA, tolB, or pal genes result in sensitivity to bile salts and the leakage of periplasmic proteins. Moreover, some of the tol genes are necessary for the entry of group A colicins and the DNA of filam...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.3.821-824.2000
更新日期:2000-02-01 00:00:00
abstract::In this study, we demonstrated that the surface-expressed enolase from diarrheal isolate SSU of Aeromonas hydrophila bound to human plasminogen and facilitated the latter's tissue-type plasminogen activator-mediated activation to plasmin. The bacterial surface-bound plasmin was more resistant to the action of its spec...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00005-09
更新日期:2009-05-01 00:00:00
abstract::The distribution of a pulse of teichoic acid-specific radiolabel between wall and membrane teichoic acids in pneumococci was constant over a subsequent chase period, suggesting that wall and membrane teichoic acids are biosynthesized simultaneously and independently. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.122.1.335-337.1975
更新日期:1975-04-01 00:00:00
abstract::Gaines, Sidney (Walter Reed Army Institute of Research, Washington, D.C.), Julius A. Currie, and Joseph G. Tully. Factors affecting formation of incomplete Vi antibody in mice. J. Bacteriol. 90:635-642. 1965.-Single immunizing doses of purified Vi antigen elicited complete and incomplete Vi antibodies in BALB/c mice, ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.90.3.635-642.1965
更新日期:1965-09-01 00:00:00
abstract::The roles of three TATA binding protein (TBP) homologs (TBP1, TBP2, and TBP3) in the archaeon Methanosarcina acetivorans were investigated by using genetic and molecular approaches. Although tbp2 and tbp3 deletion mutants were readily obtained, a tbp1 mutant was not obtained, and the growth of a conditional tbp1 expre...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01165-09
更新日期:2010-03-01 00:00:00
abstract::Phosphoenolpyruvate-dependent phosphorylation of methyl-alpha-d-glucopyranoside in Salmonella typhimurium is increased by the membrane active polypeptide antibiotic polymyxin B whereas active transport ability is abolished. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.100.3.1417-1419.1969
更新日期:1969-12-01 00:00:00
abstract::Acid phosphatase V of Aspergillus nidulans was purified by ammonium sulfate precipitation, gel filtration, and ion-exchange chromatography. The enzyme demonstrated a charge microheterogeneity on starch and acrylamide gel electrophoresis, but proved to be homogeneous on ultracentrifugation and gel filtration. Phosphata...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.110.1.246-255.1972
更新日期:1972-04-01 00:00:00
abstract::The growth cycle of Rickettsia quintana was studied for the first time in liquid culture. Growth of the microorganism in a transparent broth medium was made possible by the finding that fetal calf serum (FCS), but not calf serum (CS), satisfied the requirement of R. quintana (Fuller strain) for red blood cell lysate. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.103.1.184-190.1970
更新日期:1970-07-01 00:00:00