Abstract:
:Temperate bacteriophages play an important role in the pathogenicity of Staphylococcus aureus, for instance, by mediating the horizontal gene transfer of virulence factors. Here we established a classification scheme for staphylococcal prophages of the major Siphoviridae family based on integrase gene polymorphism. Seventy-one published genome sequences of staphylococcal phages were clustered into distinct integrase groups which were related to the chromosomal integration site and to the encoded virulence gene content. Analysis of three marker modules (lysogeny, tail, and lysis) for phage functional units revealed that these phages exhibit different degrees of genome mosaicism. The prevalence of prophages in a representative S. aureus strain collection consisting of 386 isolates of diverse origin was determined. By linking the phage content to dominant S. aureus clonal complexes we could show that the distribution of bacteriophages varied remarkably between lineages, indicating restriction-based barriers. A comparison of colonizing and invasive S. aureus strain populations revealed that hlb-converting phages were significantly more frequent in colonizing strains.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Goerke C,Pantucek R,Holtfreter S,Schulte B,Zink M,Grumann D,Bröker BM,Doskar J,Wolz Cdoi
10.1128/JB.01804-08subject
Has Abstractpub_date
2009-06-01 00:00:00pages
3462-8issue
11eissn
0021-9193issn
1098-5530pii
JB.01804-08journal_volume
191pub_type
杂志文章abstract::Those genes in Escherichia coli defined by mutations which result in an inability to grow at high temperatures are designated htr, indicating a high temperature requirement. A new htr mutant of E. coli was isolated and characterized and is designated htrD. The htrD gene has been mapped to 19.3 min on the E. coli chrom...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.4.1240-1247.1992
更新日期:1992-02-01 00:00:00
abstract::The structure and composition of the cell walls of hyphae of Neurospora crassa were investigated by electron microscopy, chemical analysis, and X-ray diffraction both before and after progressive enzymatic degradation by snail gut enzymes, chitinase, and trypsin. The wall consists of two phases: randomly disposed skel...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.94.1.202-212.1967
更新日期:1967-07-01 00:00:00
abstract::Dobrogosz, Walter J. (University of Illinois, Urbana) and Ralph D. DeMoss. Pentose utilization by Pediococcus pentosaceus. J. Bacteriol. 85:1356-1364. 1963.-Data are presented which indicate that pentoses are metabolized by Pediococcus pentosaceus through the use of an inducible phosphoketolase pathway. The utilizatio...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.85.6.1356-1364.1963
更新日期:1963-06-01 00:00:00
abstract::Two Rhizobium phaseoli mutants, isolated previously by Tn5 mutagenesis, elicited infection threads which ceased development prematurely, usually within root hairs. These infection threads were wide, globular, and otherwise altered in morphology, compared with normal infection threads. Anatomy and division of the root ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.168.3.1392-1401.1986
更新日期:1986-12-01 00:00:00
abstract::Halocin S8 is a hydrophobic microhalocin of 36 amino acids (3,580 Da) and is the first microhalocin to be described. This peptide antibiotic is unique since it is processed from inside a much larger, 33,962-Da pro-protein. Halocin S8 is quite robust, as it can be desalted, boiled, subjected to organic solvents, and st...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.17.4951-4958.2000
更新日期:2000-09-01 00:00:00
abstract::Scanning electron microscopy and transmission electron microscopy have been used to visualize the morphology and ultrastructure of two types of microorganisms in the hindgut of the cockroach Blaberus posticus. Both organisms, designated as either short or long rods, are attached to chitinous projections from the gut w...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.123.1.336-345.1975
更新日期:1975-07-01 00:00:00
abstract::Unsaturated fatty acids having structural features which are different from those of the monoenoic acids normally synthesized by Escherichia coli can serve as growth factors for an auxotroph requiring unsaturated fatty acids. These analogues were incorporated into the phospholipids, as shown by gas-liquid and thin-lay...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.95.5.1658-1665.1968
更新日期:1968-05-01 00:00:00
abstract::This study shows that sequential introduction of drug resistance mutations substantially increased enzyme production in Paenibacillus agaridevorans The triple mutant YT478 (rsmG Gln225→stop codon, rpsL K56R, and rpoB R485H), generated by screening for resistance to streptomycin and rifampin, expressed a 1,100-fold-lar...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00188-18
更新日期:2018-08-10 00:00:00
abstract::We have used transcriptional and translational fusions between various vir gene promoters and the lacZ gene to study the regulation of vir genes. Like other vir promoters, the virA promoter was induced by acetosyringone in a virA virG-dependent fashion. In addition to being induced by acetosyringone, the virG promoter...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.9.4047-4054.1988
更新日期:1988-09-01 00:00:00
abstract::Adjacent intein fragments fused to a Snf2/Rad54 helicase-related protein and Snf2/Rad54 helicase were reported for Deinococcus radiodurans R1, leading to the speculation that a frameshift was required for splicing or that trans splicing occurred. However, a type strain (ATCC 13939, RF18410) yielded a single protein th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.22.6387-6388.2002
更新日期:2002-11-01 00:00:00
abstract::A 3.3-kilobase PvuII fragment carrying the PHR1 gene of Saccharomyces cerevisiae has been cloned into an Escherichia coli expression vector and introduced into E. coli strains deficient in DNA photolyase. Complementation of the E. coli phr-1 mutation was observed, strongly suggesting that the yeast PHR1 gene encodes a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.161.2.769-771.1985
更新日期:1985-02-01 00:00:00
abstract::Tn4551, a clindamycin resistance (Ccr) transposon from the R plasmid pBI136, was cloned onto an Escherichia coli-Bacteroides shuttle vector which could replicate normally in E. coli but was maintained unstably in Bacteroides fragilis. To aid in cloning and to ensure maintenance of Tn4551 in E. coli, a kanamycin resist...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.8.3450-3457.1987
更新日期:1987-08-01 00:00:00
abstract::The nucleotide second messengers pppGpp and ppGpp [(p)ppGpp] are responsible for the global downregulation of transcription, translation, DNA replication, and growth rate that occurs during the stringent response. More recent studies suggest that (p)ppGpp is also an important effector in many nonstringent processes, i...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.02063-14
更新日期:2015-01-01 00:00:00
abstract::Pentaerythritol tetranitrate reductase, which reductively liberates nitrite from nitrate esters, is related to old yellow enzyme. Pentaerythritol tetranitrate reductase follows a ping-pong mechanism with competitive substrate inhibition by NADPH, is strongly inhibited by steroids, and is capable of reducing the unsatu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.22.6623-6627.1996
更新日期:1996-11-01 00:00:00
abstract::Previous determination of the nucleotide sequence of the iol region of the Bacillus subtilis genome allowed us to predict the structure of the iol operon for myo-inositol catabolism, consisting of 10 iol genes (iolA to iouJ); iolG corresponds to idh, encoding myo-inositol 2-dehydrogenase (Idh). Primer extension analys...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.14.4591-4598.1997
更新日期:1997-07-01 00:00:00
abstract::Analysis of the genome sequence of Enterococcus faecalis allowed the identification of two genes whose protein products showed 33 and 34% identity with those of sigV and yrhM of Bacillus subtilis, respectively. These genes, named sigV and rsiV, are predicted to encode members of the extracytoplasmic function subfamily...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.3.1022-1035.2005
更新日期:2005-02-01 00:00:00
abstract::Recombinant Ralstonia eutropha strain PHB(-)4 expressing the broad-substrate-specificity polyhydroxyalkanoate (PHA) synthase 1 from Pseudomonas sp. strain 61-3 (PhaC1Ps) synthesizes a PHA copolymer containing the branched side-chain unit 3-hydroxy-4-methylvalerate (3H4MV), which has a carbon backbone identical to that...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.02474-14
更新日期:2015-04-01 00:00:00
abstract::When Legionella pneumophila grows on agar plates, it secretes a surfactant that promotes flagellum- and pilus-independent "sliding" motility. We isolated three mutants that were defective for surfactant. The first two had mutations in genes predicted to encode cytoplasmic enzymes involved in lipid metabolism. These ge...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.05405-11
更新日期:2011-11-01 00:00:00
abstract::The yfdXWUVE operon appears to encode proteins that enhance the ability of Escherichia coli MG1655 to survive under acidic conditions. Although the molecular mechanisms underlying this phenotypic behavior remain to be elucidated, findings from structural genomic studies have shown that the structure of YfdW, the prote...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01823-07
更新日期:2008-04-01 00:00:00
abstract::Agrobacterium tumefaciens R10 was mutagenized by using the promoter probe transposon Tn5-gusA7, and a library of approximately 5,000 transcriptional fusions was screened for octopine-inducible patterns of gene expression. Twenty-one mutants carrying strongly inducible gusA fusions, 20 of which showed defects in the ca...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.7.1872-1880.1996
更新日期:1996-04-01 00:00:00
abstract::The methylenetetrahydrofolate dehydrogenase of the amethopterin-resistant strain Streptococcus faecium var. durans A(k) was purified 100-fold. Because it is extremely labile, this enzyme required protection by 1 mm nicotinamide adenine dinucleotide phosphate (NADP(+)) during purification; 0.01 mm EADP(+) with 0.1% bov...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.95.5.1779-1789.1968
更新日期:1968-05-01 00:00:00
abstract::L-Glutamate decarboxylase (GAD) activity of Clostridium perfringens (ATCC 8009) cells grown in various culture conditions was investigated. Remarkable variations of GAD level occur during the growth cycle in thioglycollate broth. These changes are affected by the pH of the culture medium. Addition of alkali to the cul...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.123.3.1115-1123.1975
更新日期:1975-09-01 00:00:00
abstract::Cells of Saccharomyces cerevisiae permeabilized by treatment with ether take up and incorporate exogenous deoxynucleoside triphosphate into deoxyribonucleic acid (DNA). With rho(+) strains, more than 95% of the product was mitochondrial DNA (mtDNA). This report characterizes ether-permeabilized yeast cells and describ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.140.2.333-341.1979
更新日期:1979-11-01 00:00:00
abstract::Escherichia coli W3110 contains four types of IS1 elements in the chromosome. Using an insertion element entrapping system, we collected 116 IS1 plasmid insertion mutants, which resulted from a minimum of 26 independent IS1 insertion events. All of them had insertions of IS1 of the IS1A (IS1E and IS1G) type. Inspectio...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.19.5279-5283.1998
更新日期:1998-10-01 00:00:00
abstract::Anthranilate (2-aminobenzoate) is an important intermediate in tryptophan metabolism. In order to investigate the degradation of tryptophan through anthranilate by Burkholderia cepacia, several plasposon mutations were constructed of strain DBO1 and one mutant with the plasposon insertion in the anthranilate dioxygena...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.19.5871-5881.2003
更新日期:2003-10-01 00:00:00
abstract::The phosphate moiety of D-mannitol-1-phosphate in Escherichia coli is subject to rapid turnover and is in close equilibrium with Pi and the phosphorus of fructose-1,6-bisphosphate. These three compounds account for the bulk of 32P label found in cells after several minutes of uptake of 32Pi and mannitol-1-phosphate re...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.158.1.63-68.1984
更新日期:1984-04-01 00:00:00
abstract::Nitrate reductase extracted from the membrane of Escherichia coli by alkaline heat treatment was purified to homogeneity and used to prepare specific antibody. Nitrate reductase, precipitated by this antibody from Triton extracts of the membrane, contained a third subunit not present in the purified enzyme used to pre...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.121.3.1102-1110.1975
更新日期:1975-03-01 00:00:00
abstract::Coxiella burnetii is a Gram-negative, obligate intracellular bacterial pathogen that resides within the harsh, acidic confines of a lysosome-like compartment of the host cell that is termed a parasitophorous vacuole. In this study, we characterized a thiol-specific peroxidase of C. burnetii that belongs to the atypica...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01324-09
更新日期:2010-04-01 00:00:00
abstract::The global regulatory protein Crc is involved in the repression of several catabolic pathways for sugars, hydrocarbons, and nitrogenated and aromatic compounds in Pseudomonas putida and Pseudomonas aeruginosa when other preferred carbon sources are present in the culture medium (catabolite repression), therefore modul...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.11.3678-3686.2005
更新日期:2005-06-01 00:00:00
abstract::Streptomyces clavuligerus produces at least five different clavam metabolites, including clavulanic acid and the methionine antimetabolite, alanylclavam. In vitro transposon mutagenesis was used to analyze a 13-kb region upstream of the known paralogue gene cluster. The paralogue cluster includes one group of clavulan...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00698-08
更新日期:2008-12-01 00:00:00