Diversity of prophages in dominant Staphylococcus aureus clonal lineages.

Abstract:

:Temperate bacteriophages play an important role in the pathogenicity of Staphylococcus aureus, for instance, by mediating the horizontal gene transfer of virulence factors. Here we established a classification scheme for staphylococcal prophages of the major Siphoviridae family based on integrase gene polymorphism. Seventy-one published genome sequences of staphylococcal phages were clustered into distinct integrase groups which were related to the chromosomal integration site and to the encoded virulence gene content. Analysis of three marker modules (lysogeny, tail, and lysis) for phage functional units revealed that these phages exhibit different degrees of genome mosaicism. The prevalence of prophages in a representative S. aureus strain collection consisting of 386 isolates of diverse origin was determined. By linking the phage content to dominant S. aureus clonal complexes we could show that the distribution of bacteriophages varied remarkably between lineages, indicating restriction-based barriers. A comparison of colonizing and invasive S. aureus strain populations revealed that hlb-converting phages were significantly more frequent in colonizing strains.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Goerke C,Pantucek R,Holtfreter S,Schulte B,Zink M,Grumann D,Bröker BM,Doskar J,Wolz C

doi

10.1128/JB.01804-08

subject

Has Abstract

pub_date

2009-06-01 00:00:00

pages

3462-8

issue

11

eissn

0021-9193

issn

1098-5530

pii

JB.01804-08

journal_volume

191

pub_type

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