Rapid turnover of mannitol-1-phosphate in Escherichia coli.

Abstract:

:The phosphate moiety of D-mannitol-1-phosphate in Escherichia coli is subject to rapid turnover and is in close equilibrium with Pi and the phosphorus of fructose-1,6-bisphosphate. These three compounds account for the bulk of 32P label found in cells after several minutes of uptake of 32Pi and mannitol-1-phosphate represents some 30% of this label. Mannitol-1-phosphate occurs in E. coli grown on a variety of carbon sources, in the absence of D-mannitol, and is synthesized de novo even in mutants lacking mannitol-1-phosphate dehydrogenase. The mannitol moiety of mannitol-1-phosphate was not affected during the total chase of the P moiety, which exchanged with a half-life of about 30 s. These findings suggest that the rapid equilibration of the phosphorus is a function of an enzyme, possibly a component of the phosphotransferase system, capable of forming a complex that allows the exchange of the phosphate without the equilibration of the mannitol moiety with free mannitol.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Rosenberg H,Pearce SM,Hardy CM,Jacomb PA

doi

10.1128/JB.158.1.63-68.1984

subject

Has Abstract

pub_date

1984-04-01 00:00:00

pages

63-8

issue

1

eissn

0021-9193

issn

1098-5530

journal_volume

158

pub_type

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