Characterization of the upper pathway genes for fluorene metabolism in Terrabacter sp. strain DBF63.

Abstract:

:Genes involved in the degradation of fluorene to phthalate were characterized in the fluorene degrader Terrabacter sp. strain DBF63. The initial attack on both fluorene and 9-fluorenone was catalyzed by DbfA to yield 9-fluorenol and 1,1a-dihydroxy-1-hydro-9-fluorenone, respectively. The FlnB protein exhibited activities against both 9-fluorenol and 1,1a-dihydroxy-1-hydro-9-fluorenone to produce 9-fluorenone and 2'-carboxy-2,3-dihydroxybiphenyl, respectively. FlnD is a heteromeric protein encoded by flnD1 and ORF16, being a member of the class III two-subunit extradiol dioxygenase. FlnE was identified as a serine hydrolase for the meta-cleavage products that yield phthalate.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Habe H,Chung JS,Kato H,Ayabe Y,Kasuga K,Yoshida T,Nojiri H,Yamane H,Omori T

doi

10.1128/JB.186.17.5938-5944.2004

keywords:

subject

Has Abstract

pub_date

2004-09-01 00:00:00

pages

5938-44

issue

17

eissn

0021-9193

issn

1098-5530

pii

186/17/5938

journal_volume

186

pub_type

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