Protein export by a gram-negative bacterium: production of aerolysin by Aeromonas hydrophila.

Abstract:

:The synthesis and export of aerolysin, an extracellular protein toxin released by the gram-negative bacterium Aeromonas hydrophila, was studied by pulse-labeling with [35S]methionine. The toxin was synthesized as a higher-molecular-weight precursor. This was processed cotranslationally, resulting in the appearance within the cell of the mature protein, which was then exported to the supernatant. Precursor aerolysin accumulated in cells incubated in the presence of carbonyl cyanide m-chlorophenyl hydrazone, a substance which also inhibited the export of mature aerolysin from the cell. The entrapped mature toxin could not be shocked from the cells, although it could be digested by protease applied to shocked cells. The toxin was processed and translocated across the inner membrane of pleiotropic export mutants and accumulated in the periplasm. The results indicate that more than one step is required for the export of the protein and that aerolysin does not cross the inner and outer membranes simultaneously.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Howard SP,Buckley JT

doi

10.1128/JB.161.3.1118-1124.1985

subject

Has Abstract

pub_date

1985-03-01 00:00:00

pages

1118-24

issue

3

eissn

0021-9193

issn

1098-5530

journal_volume

161

pub_type

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