Genetic and molecular analyses of the SUP201 gene: a tRNA(3Arg) nonsense suppressor of yeast cyrl-2.

Abstract:

:The temperature-sensitive cyr1-2 mutant in Saccharomyces cerevisiae produces low levels of adenylate cyclase and cyclic AMP at 25 degrees C and is unable to synthesize repressible acid phosphatase at 25 degrees C. Suppressor mutants of cyr1-2 were isolated by detecting acid phosphatase activity. One of the dominant suppressor mutations isolated was designated SUP201 and characterized. The SUP201 mutant gene was isolated from a gene library made from cyr1-2 SUP201 mutant DNA. Nucleotide sequence analysis of the cloned SUP201 gene revealed that the SUP201 gene was a mutated tRNA gene flanking GCN4, which worked as a UGA suppressor.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Morishita T,Uno I

doi

10.1128/jb.173.7.2406-2408.1991

subject

Has Abstract

pub_date

1991-04-01 00:00:00

pages

2406-8

issue

7

eissn

0021-9193

issn

1098-5530

journal_volume

173

pub_type

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