Abstract:
:Strains of Escherichia coli K-12 mutant in the genes controlling excision repair (uvr) and genetic recombination (rec) have been studied with reference to their radiosensitivity and their ability to repair X-ray-induced single-strand breaks in deoxyribonucleic acid (DNA). Mutations in the rec genes appreciably increase the radiosensitivity of E. coli K-12, whereas uvr mutations produce little if any increase in radiosensitivity. For a given dose of X-rays, the yield of single-strand breaks has been shown by alkaline sucrose gradient studies to be largely independent of the presence of rec or uvr mutations. The rec(+) cells (including those carrying the uvrB5 mutation) could efficiently rejoin X-ray-induced single-strand breaks in DNA, whereas recA56 mutants could not repair these breaks to any great extent. The recB21 and recC22 mutants showed some indication of repair capacity. From these studies, it is concluded that a correlation exists between the inability to repair single-strand breaks and the radiosensitivity of the rec mutants of E. coli K-12. This suggests that unrepaired single-strand breaks may be lethal lesions in E. coli.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Kapp DS,Smith KCdoi
10.1128/JB.103.1.49-54.1970subject
Has Abstractpub_date
1970-07-01 00:00:00pages
49-54issue
1eissn
0021-9193issn
1098-5530journal_volume
103pub_type
杂志文章abstract::Sharon, Nehama (University of Notre Dame, Notre Dame, Ind.), Morris Pollard, and W. R. Klemm. Psittacosis-infected cell system for studies on radiation damage. J. Bacteriol. 86:1139-1144. 1963.-The unique replication pattern of the agent of psittacosis in cells provides a means for studying biological effects of radia...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.86.6.1139-1144.1963
更新日期:1963-12-01 00:00:00
abstract::The effects of bdellovibrio infection on host permeability and respiration were investigated by measuring respiration rates and the rate of o-nitrophenyl-beta-d-galactopyranoside hydrolysis during the course of single infection cycles of Bdellovibrio bacteriovorus strain 109 growing on Escherichia coli ML 35 (lac i(-)...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.102.1.149-160.1970
更新日期:1970-04-01 00:00:00
abstract::The biosynthesis of the thaxtomin cyclic dipeptide phytotoxins proceeds nonribosomally via the thiotemplate mechanism. Acyladenylation, thioesterification, N-methylation, and cyclization of two amino acid substrates are catalyzed by the txtAB-encoded thaxtomin synthetase. Nucleotide sequence analysis of the region 3' ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.7.2019-2029.2002
更新日期:2002-04-01 00:00:00
abstract::The Escherichia coli rho 026 mutation that alters the transcription termination protein Rho prevents growth of wild-type bacteriophage T4. Among the consequences of this mutation are delayed and reduced T4 DNA replication. We show that these defects can be explained by defective synthesis of certain T4 replication-rec...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.7.3872-3880.1989
更新日期:1989-07-01 00:00:00
abstract::ruvC mutants of Escherichia coli appear to lack an activity that resolves Holliday intermediates into recombinant products. Yet, these strains produce close to normal numbers of recombinants in genetic crosses. This recombination proficiency was found to be a function of recG. A "mini-kan" insertion in recG was introd...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.17.5414-5418.1991
更新日期:1991-09-01 00:00:00
abstract::A number of polyacrylamide gel systems and solubilization procedures were studied to define the number and nature of "major" polypeptide bands in the outer membrane of Pseudomonas aeruginosa. It was shown that five of the eight major outer membrane proteins were "heat modifiable" in that their mobility on sodium dodec...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.140.3.902-910.1979
更新日期:1979-12-01 00:00:00
abstract::The deacetylcephalosporin C synthetase (hydroxylase) gene from Streptomyces clavuligerus has been cloned and sequenced. The open reading frame codes for a protein with an Mr of 34,584. The hydroxylase gene (cefF) is closely linked to the epimerase gene (cefD) and the expandase gene (cefE) and is transcribed in the opp...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.1.398-400.1991
更新日期:1991-01-01 00:00:00
abstract::We report the draft genome sequence of Paenisporosarcina sp. strain TG-20, which is 4.12 Mb in size and consists of 4,071 protein-coding genes and 76 RNA genes. The genome sequence of Paenisporosarcina sp. TG-20 may provide useful information about molecular adaptations that enhance survival in icy subsurface environm...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01472-12
更新日期:2012-12-01 00:00:00
abstract::Bacillus brevis 47 contains two cell wall proteins termed the outer wall protein (OWP) and middle wall protein (MWP), each of which forms hexagonal arrays in the cell wall. A 6-kilobase BglII-BclI fragment of B. brevis 47 DNA cloned into Bacillus subtilis with a derivative of pHW1 as a vector directed the synthesis of...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.168.1.365-373.1986
更新日期:1986-10-01 00:00:00
abstract::Studies of gene expression using fusions to lacZ demonstrated that the Bacillus subtilis dacB gene, encoding penicillin-binding protein 5*, is in an operon with two downstream genes, spmA and spmB. Mutations affecting any one of these three genes resulted in the production of spores with reduced heat resistance. The c...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.16.4721-4729.1995
更新日期:1995-08-01 00:00:00
abstract::Sixty-four mutants were isolated from the LT-2 wild-type strain of Salmonella typhimurium by selecting for chromate resistance. The majority of lesions were shown to lie in the cysA gene. (i) The mutants cannot take up sulfate, a finding which verifies the role of cysA in sulfate transport. In addition, 52 sulfate-tra...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.105.3.1053-1062.1971
更新日期:1971-03-01 00:00:00
abstract::Klein, Dolph (Rutgers, The State University, New Brunswick, N. J.), and David Pramer. Bacterial dissimilation of streptomycin. J. Bacteriol. 82:505-510. 1961.-The influence of various nutritional and environmental factors on the dissimilation of streptomycin by a pseudomonad isolated from soil was investigated, and co...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.82.4.505-510.1961
更新日期:1961-10-01 00:00:00
abstract::Cytochrome bd oxidase operons from more than 50 species of bacteria contain a short gene encoding a small protein that ranges from ∼30 to 50 amino acids and is predicted to localize to the cell membrane. Although cytochrome bd oxidases have been studied for more than 70 years, little is known about the role of this sm...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00324-13
更新日期:2013-08-01 00:00:00
abstract::The mechanisms by which hyperthermophilic Archaea, such as "Pyrococcus abyssi" and Pyrococcus furiosus, survive high doses of ionizing gamma irradiation are not thoroughly elucidated. Following gamma-ray irradiation at 2,500 Gy, the restoration of "P. abyssi" chromosomes took place within chromosome fragmentation. DNA...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.13.3958-3961.2003
更新日期:2003-07-01 00:00:00
abstract::Whether or not bacteria form coherent evolutionary groups via means of genetic exchange and, hence, elicit distinct species boundaries remains an unsettled issue. A recent report implied that not only may the former be true but also, in fact, the clearly distinct Campylobacter jejuni and Campylobacter coli species may...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00519-09
更新日期:2009-09-01 00:00:00
abstract::Endoribonuclease RNase E appears to control the rate-limiting step that mediates the degradation of many mRNA species in bacteria. In this work, an RNase E-like activity in Archaea is described. An endoribonucleolytic activity from the extreme halophile Haloarcula marismortui showed the same RNA substrate specificity ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.4.1180-1185.1997
更新日期:1997-02-01 00:00:00
abstract:UNLABELLED:Rhodopseudomonas palustris is an alphaproteobacterium that has served as a model organism for studies of photophosphorylation, regulation of nitrogen fixation, production of hydrogen as a biofuel, and anaerobic degradation of aromatic compounds. This bacterium is able to transition between anaerobic photoaut...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00771-15
更新日期:2015-12-28 00:00:00
abstract::Fowler, Audree V. (University of California, Los Angeles), and Irving Zabin. Effects of dimethylsulfoxide on the lactose operon in Escherichia coli. J. Bacteriol. 92:353-357. 1966.-Dimethylsulfoxide (DMSO) at a concentration of 5% (v/v) in the culture medium inhibits the growth of Escherichia coli to only a slight ext...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.92.2.353-357.1966
更新日期:1966-08-01 00:00:00
abstract::The N-terminal amino acid sequence of the polyhydroxyalkanoic acid (PHA) granule-associated M(r)-15,500 protein of Rhodococcus ruber (the GA14 protein) was analyzed. The sequence revealed that the corresponding structural gene is represented by open reading frame 3, encoding a protein with a calculated M(r) of 14,175 ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.14.4328-4337.1994
更新日期:1994-07-01 00:00:00
abstract::Fitz-James, Philip C. (University of Western Ontario, London, Ont., Canada). Morphology of spore development in Clostridium pectinovorum. J. Bacteriol. 84:104-114. 1962-The process of spore formation in Clostridium pectinovorum was followed by phase-contrast microscopy and by thin-section electron microscopy employing...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.84.1.104-114.1962
更新日期:1962-07-01 00:00:00
abstract::The properties of homogeneous preparations of carbamoylphosphate synthetase (CPSase) from wild-type Salmonella typhimurium and a cold-sensitive derivative grown at different growth temperatures were examined. For the cold-sensitive mutant, the affinity for glutamine of the form of CPSase synthesized at 20 degrees C wa...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.9.5089-5096.1990
更新日期:1990-09-01 00:00:00
abstract::The butyrogenic genes from Clostridium difficile DSM 1296(T) have been cloned and expressed in Escherichia coli. The enzymes acetyl-coenzyme A (CoA) C-acetyltransferase, 3-hydroxybutyryl-CoA dehydrogenase, crotonase, phosphate butyryltransferase, and butyrate kinase and the butyryl-CoA dehydrogenase complex composed o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00321-13
更新日期:2013-08-01 00:00:00
abstract::Shiga toxin (STX), a bacterial toxin produced by Shigella dysenteriae type 1, is a hexamer composed of five receptor-binding B subunits which encircle an alpha-helix at the carboxyl terminus of the enzymatic A polypeptide. Hybrid toxins constructed by fusing the A polypeptide sequences of STX and Shiga-like toxin type...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.11.3128-3132.1995
更新日期:1995-06-01 00:00:00
abstract::The structural gene for histidyl-tRNA synthetase was localized to 53.8 min on the Escherichia coli genome. The gene order in this region was determined to be dapE-purC-upp-purG-(guaA, guaB)-hisS-glyA. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.138.1.264-267.1979
更新日期:1979-04-01 00:00:00
abstract::Lipopolysaccharide (LPS) was isolated from the unicellular blue-green bacterium Agmenellum quadruplicatum using the procedure of Westphal and Jann (1965). It was composed of a lipid A and polysaccharide region suggesting a similarity to other gram-negative LPSs. Chemical analyses demonstrated the presence of glucose, ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.124.3.1566-1573.1975
更新日期:1975-12-01 00:00:00
abstract::The riboflavin analog roseoflavin is an antibiotic produced by Streptomyces davawensis. Riboflavin transporters are responsible for roseoflavin uptake by target cells. Roseoflavin is converted to the flavin mononucleotide (FMN) analog roseoflavin mononucleotide (RoFMN) by flavokinase and to the flavin adenine dinucleo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00646-13
更新日期:2013-09-01 00:00:00
abstract::A 12.6-kb DNA fragment from Streptomyces glaucescens GLA.0 containing the 12 genes for tetracenomycin (TCM) C biosynthesis and resistance enabled Streptomyces lividans to produce TCM C. Transcriptional analysis of the tcmPG intergenic region in this cluster established the presence of two divergent promoters. The tcmI...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.12.3887-3892.1993
更新日期:1993-06-01 00:00:00
abstract::Flavin-based electron bifurcation has recently been characterized as an essential energy conservation mechanism that is utilized by hydrogenotrophic methanogenic Archaea to generate low-potential electrons in an ATP-independent manner. Electron bifurcation likely takes place at the flavin associated with the α subunit...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00895-13
更新日期:2013-11-01 00:00:00
abstract::The control of men gene expression during growth and sporulation of Bacillus subtilis was examined at the transcriptional level. Two different approaches were used. (i) Steady-state levels of men-specific mRNA were measured directly. (ii) A men'-lacZ gene fusion was constructed. In both cases, it was observed that men...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.6.2742-2748.1988
更新日期:1988-06-01 00:00:00
abstract::A psychrotolerant marine bacterial strain, designated JUB59(T), was isolated from Antarctic surface seawater and classified as a new species of the genus Bizionia. Here, we present the first draft genome sequence for this genus, which suggests interesting features such as UV resistance, hydrolytic exoenzymes, and nitr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.06245-11
更新日期:2011-12-01 00:00:00