Transcriptional analysis of the Streptomyces glaucescens tetracenomycin C biosynthesis gene cluster.

Abstract:

:A 12.6-kb DNA fragment from Streptomyces glaucescens GLA.0 containing the 12 genes for tetracenomycin (TCM) C biosynthesis and resistance enabled Streptomyces lividans to produce TCM C. Transcriptional analysis of the tcmPG intergenic region in this cluster established the presence of two divergent promoters. The tcmIc mutation, a T-to-G transversion in the -10 region of the tcmG promoter, decreased promoter activity drastically at the stationary growth stage and time of maximum TCM C accumulation. This promoter may direct the transcription of a tcmGHIJKLMNO operon, while the other promoter is for tcmP.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Decker H,Hutchinson CR

doi

10.1128/jb.175.12.3887-3892.1993

subject

Has Abstract

pub_date

1993-06-01 00:00:00

pages

3887-92

issue

12

eissn

0021-9193

issn

1098-5530

journal_volume

175

pub_type

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