Abstract:
:The antibiotic d-cycloserine is an effective inhibitor of alanine racemase. The lack of inhibition by l-cycloserine of alanine racemase from Staphylococcus aureus led Roze and Strominger to formulate the cycloserine hypothesis. This hypothesis states that d-cycloserine has the conformation required of the substrates on the enzyme surface and that l-cycloserine cannot have this conformation. Alanine racemase from Escherichia coli W has been examined to establish whether these observations are a general feature of all alanine racemases. The enzyme (molecular weight = 95,000) has Michaelis-Menten constants of 4.6 x 10(-4)m and 9.7 x 10(-4)m for d- and l-alanine, respectively. The ratio of V(max) in the d- to l-direction is 2.3. The equilibrium constant calculated from the Haldane relationship is 1.11 +/- 0.15. Both d- and l-cycloserine are competitive inhibitors with constants (K(i)) of 6.5 x 10(-4)m and 2.1 x 10(-3)m, respectively. The ratio of K(m)d-alanine to K(i)d-cycloserine is 0.71, and the ratio of K(m)l-alanine to K(i)l-cycloserine is 0.46. Since l-cycloserine is an effective inhibitor, it is concluded that the cycloserine hypothesis does not apply to the enzyme from E. coli W.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Lambert MP,Neuhaus FCdoi
10.1128/JB.110.3.978-987.1972subject
Has Abstractpub_date
1972-06-01 00:00:00pages
978-87issue
3eissn
0021-9193issn
1098-5530journal_volume
110pub_type
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
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更新日期:1981-05-01 00:00:00
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
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doi:10.1128/jb.172.11.6282-6290.1990
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journal_title:Journal of bacteriology
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更新日期:1998-04-01 00:00:00
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更新日期:1974-01-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:2012-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.160.2.755-763.1984
更新日期:1984-11-01 00:00:00
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journal_title:Journal of bacteriology
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doi:10.1128/JB.82.1.106-109.1961
更新日期:1961-07-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.112.3.1033-1039.1972
更新日期:1972-12-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.98.2.827-828.1969
更新日期:1969-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.149.2.542-547.1982
更新日期:1982-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:1969-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:1998-05-01 00:00:00
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更新日期:1995-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1992-11-01 00:00:00
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更新日期:1993-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1979-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1993-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:2002-06-01 00:00:00
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pub_type: 杂志文章
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更新日期:1981-01-01 00:00:00