Crystal structures of active fully assembled substrate- and product-bound complexes of UDP-N-acetylmuramic acid:L-alanine ligase (MurC) from Haemophilus influenzae.

abstract：:Growth of Escherichia coli B in simple media enriched with Mn(II) resulted in the elevation of the manganese-containing superoxide dismutase, whereas growth in such medium enriched with iron caused increased content of the iron-containing superoxide dismutase. Enrichment of the medium with Co(II), Cu(II), Mo(VI), Zn(I...

journal_title：Journal of bacteriology

authors： Pugh SY,DiGuiseppi JL,Fridovich I

更新日期：1984-10-01 00:00:00

abstract：:Three evolutionarily distinct families of replicative DNA polymerases, designated polymerase B (Pol B), Pol C, and Pol D, have been identified. Members of the Pol B family are present in all three domains of life, whereas Pol C exists only in Bacteria and Pol D exists only in Archaea. Pol B enzymes replicate eukaryoti...

journal_title：Journal of bacteriology

authors： Cubonová L,Richardson T,Burkhart BW,Kelman Z,Connolly BA,Reeve JN,Santangelo TJ

更新日期：2013-05-01 00:00:00

abstract：:Integral membrane proteins from over 20 ubiquitous families of channels, secondary carriers, and primary active transporters were analyzed for average size differences between homologues from the three domains of life: Bacteria, Archaea, and Eucarya. The results showed that while eucaryotic homologues are consistently...

journal_title：Journal of bacteriology

authors： Chung YJ,Krueger C,Metzgar D,Saier MH Jr

更新日期：2001-02-01 00:00:00

abstract：:The transport of the tricarboxylic acid cycle C(4)-dicarboxylic acids was studied in both the wild-type strain and tricarboxylic acid cycle mutants of Bacillus subtilis. Active transport of malate, fumarate, and succinate was found to be inducible by these dicarboxylic acids or by precursors to them, whereas glucose o...

journal_title：Journal of bacteriology

authors： Ghei OK,Kay WW

更新日期：1973-04-01 00:00:00

abstract：:The group A Streptococcus (GAS) sof gene encodes the serum opacity factor protein, which is capable of opacifying mammalian sera and binding at least two host proteins, fibronectin and fibrinogen. The sof gene exists in approximately 50% of clinical isolates, and there is a classical association of so-called nephritog...

journal_title：Journal of bacteriology

authors： Jeng A,Sakota V,Li Z,Datta V,Beall B,Nizet V

更新日期：2003-02-01 00:00:00

abstract：:The region upstream of the Escherichia coli bgl operon is an insertion hot spot for several transposons. Elements as distantly related as Tn1, Tn5, and phage Mu home in on this location. To see what characteristics result in a high-affinity site for transposition, we compared in vivo and in vitro Mu transposition patt...

journal_title：Journal of bacteriology

authors： Manna D,Wang X,Higgins NP

更新日期：2001-06-01 00:00:00

abstract：:In the thermophilic archaeon Methanobacterium thermoautotrophicum Marburg, the structural gene for isoleucyl-tRNA synthetase (ileS) is flanked upstream by orf401 and downstream by purL. orf401 encodes a 43.5-kDa protein with an unknown function. Northern (RNA) hybridization and S1 nuclease protection experiments showe...

journal_title：Journal of bacteriology

authors： Jenal U,Thurner C,Leisinger T

更新日期：1993-09-01 00:00:00

abstract：:We have characterized group A Streptococcus (GAS) genome-wide responses to hydrogen peroxide and assessed the role of the peroxide response regulator (PerR) in GAS under oxidative stress. Comparison of transcriptome changes elicited by peroxide in wild-type bacteria with those in a perR deletion mutant showed that 76 ...

journal_title：Journal of bacteriology

authors： Grifantini R,Toukoki C,Colaprico A,Gryllos I

更新日期：2011-12-01 00:00:00

abstract：:Double-stranded, 1.9-kilobase-pair (kbp) DNA molecules were found in 18 strains representing three pathogenic races of Fusarium oxysporum f. sp. conglutinans. The DNA element (pFOXC1) from a race 1 strain and the DNA element (pFOXC2) from a race 2 strain were shown by restriction endonuclease mapping to be linear. pFO...

journal_title：Journal of bacteriology

authors： Kistler HC,Leong SA

更新日期：1986-08-01 00:00:00

abstract：:The activities of phosphofructokinase (PFK), fructose diphosphatase (FDP), nicotinamide adenine dinucleotide (NAD) and NAD phosphate (NADP)-linked isocitrate dehydrogenases (IDHNAD, IDHNADP), two NAD-linked glutamate dehydrogenases (GDH1, GDH2), and isocitrate lyase were studied during the development of the two pheno...

journal_title：Journal of bacteriology

authors： Ingebretsen OC,Sanner T

更新日期：1976-06-01 00:00:00

abstract：:Site-specific mutagenesis has identified amino acids involved in bR proton transport. Biophysical studies of the mutants have elucidated the roles of two membrane-embedded residues: Asp-85 serves as the acceptor for the proton from the isomerized retinylidene Schiff base, and Asp-96 participates in reprotonation of th...

journal_title：Journal of bacteriology

authors： Krebs MP,Khorana HG

更新日期：1993-03-01 00:00:00

abstract：:Accumulation of K+ ions and glutamate plays a primary role in maintaining osmotic balance in Escherichia coli, as illustrated by the high concentrations of these ions present in cells growing in medium of high osmolality. We found that two gamma-glutamyl peptides and glutamine also accumulated during growth at high os...

journal_title：Journal of bacteriology

authors： McLaggan D,Logan TM,Lynn DG,Epstein W

更新日期：1990-07-01 00:00:00

abstract：:A Rhodobacter sphaeroides mutant (CYCA1) lacking cytochrome c2 (cyt c2) was previously constructed (T. J. Donohue, A. G. McEwan, S. Van Doren, A. R. Crofts, and S. Kaplan, Biochemistry, 27: 1918-1924, 1988) by a combination of in vivo and in vitro molecular genetic techniques. CYCA1 was incapable of photosynthetic gro...

journal_title：Journal of bacteriology

authors： Brandner JP,McEwan AG,Kaplan S,Donohue TJ

更新日期：1989-01-01 00:00:00

abstract：:Pyruvate kinase of Streptococcus mutans JC 2 had an absolute and specific requirement for glucose-6-phosphate. Inorganic phosphate was a strong inhibitor. The enzyme required K+ or NH4+ and Mg2+ or Mn2+. S. mutans FIL and E 49, Streptococcus bovis ATCC 9809, and Streptococcus salivarius ATCC 13419 had also glucose-6-p...

journal_title：Journal of bacteriology

authors： Yamada T,Carlsson J

更新日期：1975-10-01 00:00:00

abstract：:Quorum sensing is involved in the regulation of multicellular behavior through communication via small molecules. Given the high number and diversity of the gastrointestinal microbiota, it is postulated that members of this community communicate to coordinate a variety of adaptive processes. AI-2 is suggested to be a ...

journal_title：Journal of bacteriology

authors： Lebeer S,De Keersmaecker SC,Verhoeven TL,Fadda AA,Marchal K,Vanderleyden J

更新日期：2007-02-01 00:00:00

abstract：:In order to assess the roles of specific amino acid residues in the delta 5-3-ketosteroid isomerase from Pseudomonas putida biotype B during catalysis, we replaced aspartic acid 40 with asparagine (D40N) and tyrosine 16 with phenylalanine (Y16F) in the enzyme by site-directed mutagenesis. Both purified mutant enzymes ...

journal_title：Journal of bacteriology

authors： Kim SW,Choi KY

更新日期：1995-05-01 00:00:00

abstract：:An apparatus has been developed in which bacterial growth can be measured very precisely over short intervals of time. Its precision is presented and used to assess the constancy of growth in batch culture. Under certain conditions, i.e., Luria broth or 0.2% glucose-M9 medium at very low cell densities, the specific g...

journal_title：Journal of bacteriology

authors： Wang CH,Koch AL

更新日期：1978-12-01 00:00:00

abstract：:In gram-negative bacteria, numerous cell functions, including respiration-linked electron transport, have been ascribed to the cytoplasmic membrane. Gram-negative bacteria which use solid substrates (e.g., oxidized manganese or iron) as terminal electron acceptors for anaerobic respiration are presented with a unique ...

journal_title：Journal of bacteriology

authors： Myers CR,Myers JM

更新日期：1992-06-01 00:00:00

abstract：:The Rnf complex is a respiratory enzyme that catalyzes the oxidation of reduced ferredoxin to the reduction of NAD+, and the negative free energy change of this reaction is used to generate a transmembrane ion gradient. In one class of anaerobic acetogenic bacteria, the Rnf complex is believed to be essential for ener...

journal_title：Journal of bacteriology

authors： Westphal L,Wiechmann A,Baker J,Minton NP,Müller V

更新日期：2018-10-10 00:00:00

abstract：:A Bordetella bronchiseptica iron transport mutant was isolated following an enrichment procedure based on streptonigrin resistance. The mutant displayed a growth defect on iron-restricted medium containing ferric alcaligin as the sole iron source. In addition to the apparent inability to acquire iron from the sideroph...

journal_title：Journal of bacteriology

authors： Beaumont FC,Kang HY,Brickman TJ,Armstrong SK

更新日期：1998-02-01 00:00:00

abstract：:Morphogenesis of the filamentous bacteriophage f1 occurred at adhesion zones between the inner and outer membranes of the host cell. Quantitation of adhesion zones in cells infected with mutant phage strains suggested that the phage gene I protein may be involved in the formation of adhesion zones for phage assembly. ...

journal_title：Journal of bacteriology

authors： Lopez J,Webster RE

更新日期：1985-09-01 00:00:00

abstract：:A 3,373-base-pair DNA segment from a clone fortuitously isolated from Saccharopolyspora erythraea by hybridization to an oligodeoxynucleotide probe was sequenced. Computer-assisted analysis of the nucleotide sequence reveals three closely linked Streptomyces open reading frames plus a fourth converging on the others. ...

journal_title：Journal of bacteriology

authors： Donadio S,Shafiee A,Hutchinson CR

更新日期：1990-01-01 00:00:00

abstract：:The growth of Bacillus subtilis mutant betaA177 can be inhibited under special conditions in which not enough autolytic enzymes are produced for optimal growth. Electron microscopy studies show that during growth inhibition there is localized thickening of the cell wall at positions where cells bend. A model is propos...

journal_title：Journal of bacteriology

authors： Fan DP,Beckman MM,Cunningham WP

更新日期：1972-03-01 00:00:00

abstract：:The effect of various salts on the autolysis of cell wall of a ribitol teichoic acid-deficient mutant of Staphylococcus aureus H (strain 52A5 carrying tar-1) was compared with the parent strain. In the presence of high concentrations of certain salts such as 1.0 m NaCl, the mutant undergoes autolysis with the release ...

journal_title：Journal of bacteriology

authors： Gilpin RW,Chatterjee AN,Young FE

更新日期：1972-07-01 00:00:00

abstract：:S1 nuclease mapping and Northern (RNA) hybridization revealed that the rpoDA gene encoding the principal sigma subunit of Pseudomonas aeruginosa PAO1 is transcribed as a monocistronic mRNA of 2 kb and that the transcription from the rpoDA promoter (PC) starts 32 bases upstream from the first nucleotide of the initiati...

journal_title：Journal of bacteriology

authors： Fujita M,Tanaka K,Takahashi H,Amemura A

更新日期：1993-02-01 00:00:00

abstract：:The lipopolysaccharide (LPS) from Rhizobium trifolii 0403 was isolated at different stages of growth and was examined for its (i) ability to bind a white clover lectin (trifoliin A), (ii) immunochemical properties, and (iii) composition. There was significantly more binding of trifoliin A to purified LPS and cells in ...

journal_title：Journal of bacteriology

authors： Hrabak EM,Urbano MR,Dazzo FB

更新日期：1981-11-01 00:00:00

abstract：:Plasmid pE194 has been shown to be rescued by integration after cultivation of infected Bacillus subtilis recE4 cells at a restrictive high temperature. The plasmid is also spontaneously excised from the chromosome at a low frequency by precise or imprecise excision (J. Hofemeister, M. Israeli-Reches, and D. Dubnau, M...

journal_title：Journal of bacteriology

authors： Conrad B,Bashkirov VI,Hofemeister J

更新日期：1992-11-01 00:00:00

abstract：:This paper describes the major transmutations of donor deoxyribonucleic acid (DNA) after uptake by competent Bacillus subtilis cells. Kinetic experiments confirm that after exposure to competent cells, donor DNA is converted to double-stranded fragments (DSF) which can be isolated as early as 30 s from the beginning o...

journal_title：Journal of bacteriology

authors： Davidoff-Abelson R,Dubnau D

更新日期：1973-10-01 00:00:00

abstract：:Lysyl-transfer ribonucleic acid synthetase (EC 6.1.1.6) was identified as four polypeptide spots after two-dimensional polyacrylamide gel electrophoresis of whole-cell lysates of Escherichia coli. Identification was made by migration with partially purified enzyme preparations, by peptide map patterns, by mutant analy...

journal_title：Journal of bacteriology

authors： Hirshfield IN,Bloch PL,Van Bogelen RA,Neidhardt FC

更新日期：1981-04-01 00:00:00

abstract：:Seventy-one methionineless and cysteineless auxotrophs of Pseudomonas aeruginosa were placed into nine groups on the basis of their growth on methionine precursors and the cross-feeding response. Transduction experiments with bacteriophage F116 indicated the presence of four linkage groups among the methionineless mut...

journal_title：Journal of bacteriology

authors： Calhoun DH,Feary TW

更新日期：1969-01-01 00:00:00