Three-dimensional structure of different functional forms of the Vibrio cholerae hemolysin oligomer: a cryo-electron microscopic study.

abstract：:The DNA transfer stage of conjugation requires the products of the F sex factor genes traMYDIZ and the cis-acting site oriT. Previous interpretation of genetic and protein analyses suggested that traD, traI, and traZ mapped as contiguous genes at the distal end of the transfer operon and saturated this portion of the ...

journal_title：Journal of bacteriology

authors： Traxler BA,Minkley EG Jr

更新日期：1987-07-01 00:00:00

abstract：:The cytoskeletal proteins HMW1 and HMW2 are components of the terminal organelle of the cell wall-less bacterium Mycoplasma pneumoniae. HMW1 is required for a tapered, filamentous morphology but exhibits accelerated turnover in the absence of HMW2. Here, we report that a reciprocal dependency exists between HMW1 and H...

journal_title：Journal of bacteriology

authors： Willby MJ,Balish MF,Ross SM,Lee KK,Jordan JL,Krause DC

更新日期：2004-12-01 00:00:00

abstract：:Under static growth conditions, hyperpiliated, nontwitching pilT and pilU mutants of Pseudomonas aeruginosa formed dense biofilms, showing that adhesion, not twitching motility, is necessary for biofilm initiation. Under flow conditions, the pilT mutant formed mushroom-like structures larger than those of the wild typ...

journal_title：Journal of bacteriology

authors： Chiang P,Burrows LL

更新日期：2003-04-01 00:00:00

abstract：:Members of all four families of methanogenic bacteria were analyzed for polyamine concentrations. High-performance liquid chromatography analysis of dansylated cell extracts revealed typical polyamine patterns for each family. Members of Methanobacteriaceae (family I) were characterized by very low polyamine concentra...

journal_title：Journal of bacteriology

authors： Scherer P,Kneifel H

更新日期：1983-06-01 00:00:00

abstract：:Resistance of Staphylococcus aureus mediated by the penicillinase (Pc-ase) plasmid to divalent metal ions of Hg and Cd was found to be controlled by different mechanisms. The Hg resistance of the Pc-ase plasmid-carrying organisms is based upon a process of changing the ion incorporated in the cell into a somewhat inno...

journal_title：Journal of bacteriology

authors： Kondo I,Ishikawa T,Nakahara H

更新日期：1974-01-01 00:00:00

abstract：:Falcone, Giuseppe (Rutgers, The State University, New Brunswick, N.J.), and Walter J. Nickerson. Reduction of selenite by intact yeast cells and cell-free preparations. J. Bacteriol. 85:754-762. 1963.-Nonproliferating cell suspensions of Candida albicans rapidly reduced selenite to red, metallic selenium in the absenc...

journal_title：Journal of bacteriology

authors： FALCONE G,NICKERSON WJ

更新日期：1963-04-01 00:00:00

abstract：:Sinorhizobium meliloti strains lacking BacA function are impaired in symbiosis with alfalfa host plants and display altered sensitivities to a number of compounds relative to wild-type strains. With the goal of finding clues to the currently unknown biological function(s) of BacA, we carried out a genetic analysis to ...

journal_title：Journal of bacteriology

authors： LeVier K,Walker GC

更新日期：2001-11-01 00:00:00

abstract：:Here we describe that the Helicobacter pylori sensor kinase produced by HP1364 and the response regulator produced by HP1365 and designated CrdS and CrdR, respectively, are both required for transcriptional induction of the H. pylori copper resistance determinant CrdA by copper ions. CrdRS-deficient mutants lacked cop...

journal_title：Journal of bacteriology

authors： Waidner B,Melchers K,Stähler FN,Kist M,Bereswill S

更新日期：2005-07-01 00:00:00

abstract：:Oleandomycin, a macrolide antibiotic produced by Streptomyces antibioticus, contains two sugars attached to the aglycon: L-oleandrose and D-desosamine. oleY codes for a methyltransferase involved in the biosynthesis of L-oleandrose. This gene was overexpressed in Escherichia coli to form inclusion bodies and in Strept...

journal_title：Journal of bacteriology

authors： Rodríguez L,Rodríguez D,Olano C,Braña AF,Méndez C,Salas JA

更新日期：2001-09-01 00:00:00

abstract：:The alkaline phosphatases of 29 strains of bacteria assigned by various authors to the genera Aerobacter, Klebsiella and Enterobacter were compared by the micro-complement fixation technique. On the basis of phosphatase resemblance, we recommend that all strains hitherto assigned to Aerobacter aerogenes and Enterobact...

journal_title：Journal of bacteriology

authors： Steffen DL,Cocks GT,Wilson AC

更新日期：1972-06-01 00:00:00

abstract：:In the nitrogenase system of Klebsiella pneumoniae, nifT is located between nifDK, the structural genes for dinitrogenase, and nifY, whose product is involved in nitrogenase maturation. It is, therefore, a reasonable hypothesis that the NifT protein might also have a role in the maturation of nitrogenase. However, the...

journal_title：Journal of bacteriology

authors： Simon HM,Homer MJ,Roberts GP

更新日期：1996-05-01 00:00:00

abstract：:A mutant of Rhizobium meliloti, 4R3, which is unable to grow on aspartate has been isolated. The defect is specific to aspartate utilization, since 4R3 is not an auxotroph and grows as well as its parent strain on other carbon and nitrogen sources. The defect was correlated with an inability to fix nitrogen within nod...

journal_title：Journal of bacteriology

authors： Rastogi VK,Watson RJ

更新日期：1991-05-01 00:00:00

abstract：:Vibrio cholerae is a motile bacterium responsible for the disease cholera, and motility has been hypothesized to be inversely regulated with virulence. We examined the transcription profiles of V. cholerae strains containing mutations in flagellar regulatory genes (rpoN, flrA, flrC, and fliA) by utilizing whole-genome...

journal_title：Journal of bacteriology

authors： Syed KA,Beyhan S,Correa N,Queen J,Liu J,Peng F,Satchell KJ,Yildiz F,Klose KE

更新日期：2009-11-01 00:00:00

abstract：:The pst gene cluster encodes the phosphate-specific transport (Pst) system. Inactivation of the Pst system constitutively activates the two-component regulatory system PhoBR and attenuates the virulence of pathogenic bacteria. In uropathogenic Escherichia coli strain CFT073, attenuation by inactivation of pst is predo...

journal_title：Journal of bacteriology

authors： Crépin S,Porcheron G,Houle S,Harel J,Dozois CM

更新日期：2017-11-14 00:00:00

abstract：:A mutation in a new gene, molR, prevented the synthesis in Escherichia coli of molybdoenzymes, including the two formate dehydrogenase isoenzymes, nitrate reductase and trimethylamine-N-oxide reductase. This phenotype was suppressed by supplementing the media with molybdate. Thus, the molR mutant was phenotypically si...

journal_title：Journal of bacteriology

authors： Lee JH,Wendt JC,Shanmugam KT

更新日期：1990-04-01 00:00:00

abstract：:The ligated loop of the rabbit intestine was investigated as a possible experimental model for the study of Clostridium perfringens food poisoning. The method of preparation of the challenge inoculum was important in determining whether a given strain would provoke a response. When cultures were grown for 4 hr at 37 C...

journal_title：Journal of bacteriology

authors： Duncan CL,Sugiyama H,Strong DH

更新日期：1968-05-01 00:00:00

abstract：:The hook-basal body complex of Salmonella typhimurium, a major component of its flagellar apparatus, was subjected to detailed analysis by electron microscopy and gel electrophoresis. The study was facilitated by the development of an improved protocol for isolation of the complexes in high yield and purity. Nine prot...

journal_title：Journal of bacteriology

authors： Aizawa SI,Dean GE,Jones CJ,Macnab RM,Yamaguchi S

更新日期：1985-03-01 00:00:00

abstract：:A segment of Escherichia coli DNA that contained a discontinuity of homology with Salmonella typhimurium DNA was isolated. The segment, 1,430 base pairs long, was derived from one end of the lac "loop," a region of about 12 kilobase pairs of E. coli DNA, including the lac operon which has no detectable homology with S...

journal_title：Journal of bacteriology

authors： Buvinger WE,Lampel KA,Bojanowski RJ,Riley M

更新日期：1984-08-01 00:00:00

abstract：:The phosphate moiety of D-mannitol-1-phosphate in Escherichia coli is subject to rapid turnover and is in close equilibrium with Pi and the phosphorus of fructose-1,6-bisphosphate. These three compounds account for the bulk of 32P label found in cells after several minutes of uptake of 32Pi and mannitol-1-phosphate re...

journal_title：Journal of bacteriology

authors： Rosenberg H,Pearce SM,Hardy CM,Jacomb PA

更新日期：1984-04-01 00:00:00

abstract：:We isolated several new mutator mutations of the Escherichia coli replicative polymerase dnaE subunit alpha and used them and a previously reported dnaE mutation to study spontaneous frameshift and base substitution mutations. Two of these dnaE strains produce many more mutants when grown on rich (Luria-Bertani) than ...

journal_title：Journal of bacteriology

authors： Strauss BS,Roberts R,Francis L,Pouryazdanparast P

更新日期：2000-12-01 00:00:00

abstract：:During spore formation, Bacillus subtilis divides asymmetrically, resulting in two cells with different fates. Immediately after division, the transcription factor σ(F) becomes active in the smaller prespore, followed by activation of σ(E) in the larger mother cell. We recently showed that a delay in σ(E) activation r...

journal_title：Journal of bacteriology

authors： Xenopoulos P,Piggot PJ

更新日期：2011-06-01 00:00:00

abstract：:Escherichia coli W3110 contains four types of IS1 elements in the chromosome. Using an insertion element entrapping system, we collected 116 IS1 plasmid insertion mutants, which resulted from a minimum of 26 independent IS1 insertion events. All of them had insertions of IS1 of the IS1A (IS1E and IS1G) type. Inspectio...

journal_title：Journal of bacteriology

authors： Chen JH,Hsu WB,Hwang JL

更新日期：1998-10-01 00:00:00

abstract：:The murG gene of Escherichia coli encodes the N-acetylglucosaminyltransferase responsible for the final step in the formation of the lipid-linked disaccharide-pentapeptide subunit of peptidoglycan. Using trypsin to probe maxicell spheroplasts, we show that this enzyme is peripherally associated with the inner face of ...

journal_title：Journal of bacteriology

authors： Bupp K,van Heijenoort J

更新日期：1993-03-01 00:00:00

abstract：:In vitro, archaeal SRP54 binds SRP RNA in the absence of SRP19, suggesting the latter to be expendable in Archaea. Accordingly, the Haloferax volcanii SRP19 gene was deleted. Although normally transcribed at a level comparable to that of the essential SRP54 gene, SRP19 deletion had no effect on cell growth, membrane p...

journal_title：Journal of bacteriology

authors： Yurist S,Dahan I,Eichler J

更新日期：2007-01-01 00:00:00

abstract：:Among its penicillin-binding proteins (PBPs), Enterococcus faecium possesses a low-affinity PBP5, PBP5fm, which is the main target involved in beta-lactam resistance. A 7.7-kb EcoRI chromosomal fragment of E. faecium D63r containing the pbp5fm gene was cloned and sequenced. Two open reading frames (ORFs) were found. A...

journal_title：Journal of bacteriology

authors： Zorzi W,Zhou XY,Dardenne O,Lamotte J,Raze D,Pierre J,Gutmann L,Coyette J

更新日期：1996-08-01 00:00:00

abstract：:As a step towards studying representative members of the two-component family of signal transduction proteins, we have cloned genes encoding a histidine protein kinase and a response regulator from the hyperthermophilic bacterium Thermotoga maritima. The genes have been designated HpkA and drrA, respectively. The dedu...

journal_title：Journal of bacteriology

authors： Lee PJ,Stock AM

更新日期：1996-10-01 00:00:00

abstract：:We have mapped a regulatory site mediating the hyperproduction of cholera toxin in mutants of Vibrio cholerae strain 569B. Mutations in this locus, called htx, result in the hypertoxinogenic phenotype, as measured by the ganglioside filter assay and immunoradial diffusion. Transposon-facilitated recombination was used...

journal_title：Journal of bacteriology

authors： Mekalanos JJ,Sublett RD,Romig WR

更新日期：1979-09-01 00:00:00

abstract：:Efficient hydrolysis of native poly(3-hydroxybutyrate) (nPHB) granules in vitro by soluble PHB depolymerase of Rhodospirillum rubrum requires pretreatment of nPHB with an activator compound present in R. rubrum cells (J. M. Merrick and M. Doudoroff, J. Bacteriol. 88:60-71, 1964). Edman sequencing of the purified activ...

journal_title：Journal of bacteriology

authors： Handrick R,Reinhardt S,Schultheiss D,Reichart T,Schüler D,Jendrossek V,Jendrossek D

更新日期：2004-04-01 00:00:00

abstract：:The global transcriptional regulator Spx of Bacillus subtilis is controlled at several levels of the gene expression process. It is maintained at low concentrations during unperturbed growth by the ATP-dependent protease ClpXP. Under disulfide stress, Spx concentration increases due in part to a reduction in ClpXP-cat...

journal_title：Journal of bacteriology

authors： Garg SK,Kommineni S,Henslee L,Zhang Y,Zuber P

更新日期：2009-02-01 00:00:00

abstract：:An ADP-ribosylating toxin named Aeromonas salmonicida exoenzyme T (AexT) in A. salmonicida subsp. salmonicida, the etiological agent of furunculosis in fish, was characterized. Gene aexT, encoding toxin AexT, was cloned and characterized by sequence analysis. AexT shows significant sequence similarity to the ExoS and ...

journal_title：Journal of bacteriology

authors： Braun M,Stuber K,Schlatter Y,Wahli T,Kuhnert P,Frey J

更新日期：2002-04-01 00:00:00