Abstract:
:Two unusual characteristics of some outer membrane proteins of Rhizobium leguminosarum are described. First, most of the major outer membrane proteins could only be visualized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis after lysozyme treatment of the isolated cell envelopes, suggesting a very strong, possibly covalent, interaction of these proteins with the peptidoglycan. These peptidoglycan-associated outer membrane proteins belonged to two distinct groups of immunologically related proteins, groups II and III, as defined by typing with monoclonal antibodies. As members of both groups of proteins could be radioactively labeled by growing cells in the presence of N-[3H]acetylglucosamine, we propose that variation in the apparent molecular weight of the antigens within each group is caused by varying numbers of peptidoglycan subunit residues on only two or three different outer membrane proteins. Second, group III outer membrane proteins, with masses of 35 to 46 kilodaltons, formed oligomers stabilized by divalent cations which resisted complete denaturation in 2% sodium dodecyl sulfate at 100 degrees C. Reconstitution experiments showed that of the divalent cations tested, Ca2+ and, to a lesser extent, Mn2+ and Sr2+ were the best stabilizers.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
de Maagd RA,Wientjes FB,Lugtenberg BJdoi
10.1128/jb.171.7.3989-3995.1989subject
Has Abstractpub_date
1989-07-01 00:00:00pages
3989-95issue
7eissn
0021-9193issn
1098-5530journal_volume
171pub_type
杂志文章abstract::Kimler, Alexander (National Institutes of Health, Bethesda, Md.). Fluorescein amine media for rapid differentiation of staphylococci. J. Bacteriol. 82:106-109. 1961.-By modifying conventional media to include blood, mannitol, and fluorescein amine, staphylococci may be isolated and identified directly. Staphylococcus ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.82.1.106-109.1961
更新日期:1961-07-01 00:00:00
abstract::RpoS, the sigma factor of enteric bacteria that responds to stress and stationary phase, is subject to complex regulation acting at multiple levels, including transcription, translation, and proteolysis. Increased translation of rpoS mRNA during growth at low temperature, after osmotic challenge, or with a constitutiv...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00206-06
更新日期:2006-07-01 00:00:00
abstract::An 846-bp cryptic plasmid has been discovered in the hyperthermophilic bacterium Thermotoga sp. strain RQ7. This is the first plasmid described for an organism from this ancient bacterial lineage and the smallest plasmid described to date for any organism. Nucleotide sequencing revealed a single open reading frame pos...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.9.2759-2762.1994
更新日期:1994-05-01 00:00:00
abstract::Methanol:5-hydroxybenzimidazolylcobamide methyltransferase (MT1) is the first of two enzymes required for transfer of the methyl group of methanol to 2-mercaptoethanesulfonic acid in Methanosarcina barkeri. MT1 binds the methyl group of methanol to its corrinoid prosthetic group only when the central cobalt atom of th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.5.1278-1283.1993
更新日期:1993-03-01 00:00:00
abstract::SbmA is an inner membrane protein of Gram-negative bacteria that is involved in the internalization of glycopeptides and prokaryotic and eukaryotic antimicrobial peptides, as well as of peptide nucleic acid (PNA) oligomers. The SbmA homolog BacA is required for the development of Sinorhizobium meliloti bacteroids with...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00818-13
更新日期:2013-12-01 00:00:00
abstract::We measured some of the kinetic parameters of nitrogenase to intact systems of Clostridium pasteurianum and Klebsiella pneumoniae to compare them with the kinetics of the enzyme in vitro. We found that the enzyme showed multiple apparent Km values for acetylene reduction in vivo, as it does in vitro. Carbon monoxide w...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.141.3.1230-1238.1980
更新日期:1980-03-01 00:00:00
abstract::The crystal structure of a small, basic DNA binding protein, Sso10b2, from the thermoacidophilic archaeon Sulfolobus solfataricus was determined by the Zn multiwavelength anomalous diffraction method and refined to 1.85 A resolution. The 89-amino-acid protein adopts a betaalphabetaalphabetabeta topology. The structure...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.14.4066-4073.2003
更新日期:2003-07-01 00:00:00
abstract::Unsaturated fatty acids having structural features which are different from those of the monoenoic acids normally synthesized by Escherichia coli can serve as growth factors for an auxotroph requiring unsaturated fatty acids. These analogues were incorporated into the phospholipids, as shown by gas-liquid and thin-lay...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.95.5.1658-1665.1968
更新日期:1968-05-01 00:00:00
abstract::Methyl coenzyme M methylreductase from acetate-grown Methanosarcina thermophila TM-1 was purified 16-fold from a cell extract to apparent homogeneity as determined by native polyacrylamide gel electrophoresis. Ninety-four percent of the methylreductase activity was recovered in the soluble fraction of cell extracts. T...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.8.2481-2487.1991
更新日期:1991-04-01 00:00:00
abstract::A thermosensitive sporulation mutant of Bacillus subtilis containing a mutation in the secY gene was isolated and characterized. No asymmetric septum specific to the sporulation was detected by electron microscopy at the nonpermissive temperature, indicating that the block occurred at a very early stage of sporulation...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.11.3656-3660.1993
更新日期:1993-06-01 00:00:00
abstract::Induction of nod genes in Rhizobium and Bradyrhizobium species is dependent on the presence of plant-produced flavonoids, the NodD protein, and the cis-acting nod box promoter sequence. Although the nodD (nodD1) gene in Rhizobium species is constitutively expressed, nodD1 expression in Bradyrhizobium japonicum is indu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.11.3356-3365.1991
更新日期:1991-06-01 00:00:00
abstract::The product of the papD gene of uropathogenic Escherichia coli is required for the biogenesis of digalactoside-binding P pili. Mutations within papD result in complete degradation of the major pilus subunit, PapA, and of the pilinlike proteins PapE and PapF and also cause partial breakdown of the PapG adhesin. The pap...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.11.6052-6058.1989
更新日期:1989-11-01 00:00:00
abstract::At various stages during spore formation sporangia were shocked by cold treatment or with toluene, and the germination requirements of the prespores were examined. Up to 5 h after induction of sporulation (t5) germination was spontaneous; i.e., it occurred without any added germinants. After t5, during stages V and VI...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.141.2.786-792.1980
更新日期:1980-02-01 00:00:00
abstract::Wolin, M. J. (University of Illinois, Urbana). Lysis of Vibrio succinogenes by ethylenediaminetetraacetic acid or lysozyme. J. Bacteriol. 91:1781-1786. 1966.-Cell suspensions of Vibrio succinogenes are lysed by ethylenediaminetetraacetic acid (EDTA) or lysozyme. Lysis occurs at alkaline pH and is prevented by 0.15 m N...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.91.5.1781-1786.1966
更新日期:1966-05-01 00:00:00
abstract::Inducible cat and erm genes are regulated by translational attenuation. In this regulatory model, gene activation results from chloramphenicol- or erythromycin-dependent stalling of a ribosome at a precise site in the leader region of cat or erm transcripts. The stalled ribosome is believed to destabilize a downstream...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.11.6282-6290.1990
更新日期:1990-11-01 00:00:00
abstract::H10407 is a strain of enterotoxigenic Escherichia coli (ETEC) that utilizes CFA/I pili to adhere to surfaces of the small intestine, where it elaborates toxins that cause profuse watery diarrhea in humans. Expression of the CFA/I pilus is positively regulated at the level of transcription by CfaD, a member of the AraC...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00131-07
更新日期:2007-07-01 00:00:00
abstract::The levels of reduced and oxidized nicotinamide adenine dinucleotides were determined in Xanthobacter flavus during a transition from heterotrophic to autotrophic growth. Excess reducing equivalents are rapidly dissipated following induction of the Calvin cycle, indicating that the Calvin cycle serves as a sink for ex...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.16.4637-4639.2000
更新日期:2000-08-01 00:00:00
abstract::Extracellular fibrils are involved in cell cohesion and cell development in Myxococcus xanthus. One group of social motility mutants, Dsp, is unable to produce extracellular fibrils; these mutants also lose the abilities to cohere and to develop. Extracellular fibrils isolated from vegetative wild-type cells and added...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.23.7190-7196.1994
更新日期:1994-12-01 00:00:00
abstract::Here, we report the completely annotated genome sequence of Streptococcus pyogenes M1 476 isolated from a patient with streptococcal toxic shock syndrome (STSS) during pregnancy. The genome sequence will provide new insights into the mechanisms underlying STSS. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01265-12
更新日期:2012-10-01 00:00:00
abstract::Banerjea, A. (Rocky Mountain Laboratory, Hamilton, Mont.) and J. Munoz. Antigens of Bordetella pertussis. II, Purification of heat-labile toxin. J. Bacteriol. 84:269-274. 1962.-A mild method of separating heat-labile toxin of Bordetella pertussis from other cellular components is described; it consists of absorbing to...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.84.2.269-274.1962
更新日期:1962-08-01 00:00:00
abstract::In contrast to previous findings, we demonstrate that the dissimilatory (bi)sulfite reductase genes (dsrAB) of Desulfobacula toluolica were vertically inherited. Furthermore, Desulfobacterium anilini and strain mXyS1 were identified, by dsrAB sequencing of 17 reference strains, as members of the donor lineage for thos...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.6.2203-2208.2005
更新日期:2005-03-01 00:00:00
abstract:UNLABELLED:Neisseria meningitidis, an exclusively human pathogen and the leading cause of bacterial meningitis, must adapt to different host niches during human infection. N. meningitidis can utilize a restricted range of carbon sources, including lactate, glucose, and pyruvate, whose concentrations vary in host niches...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00659-15
更新日期:2015-12-07 00:00:00
abstract::When Azotobacter vinelandii was derepressed for nitrogenase synthesis in a N-free medium containing tungstate instead of molybdate, an inactive component I was synthesized. Although this inactive component I could be activated in vivo upon addition of molybdate to the medium, it could not be activated in vitro when mo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.120.2.697-701.1974
更新日期:1974-11-01 00:00:00
abstract::We report a draft sequence of the genome of Gordonia neofelifaecis NRRL B-59395, a cholesterol-degrading actinomycete isolated from fresh feces of a clouded leopard (Neofelis nebulosa). As predicted, the reported genome contains several gene clusters for cholesterol degradation. This is the second available genome seq...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.05531-11
更新日期:2011-09-01 00:00:00
abstract::Cybulska, Janina (State Institute of Hygiene, Warsaw, Poland), and J. Jeljaszewicz. Bacteriostatic activity of serum against staphylococci. J. Bacteriol. 91:953-962. 1966.-Antistaphylococcal activity of normal serum against strains exhibiting various patterns of coagulase, clumping-factor, and staphylokinase productio...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.91.3.953-962.1966
更新日期:1966-03-01 00:00:00
abstract::Bacteria form communities, termed biofilms, in which cells adhere to each other within a matrix, typically comprised of polysaccharides, proteins, and extracellular DNA. Biofilm formation by the marine bacterium Vibrio fischeri requires the Syp polysaccharide, but the involvement of matrix proteins is as yet unknown. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.02292-14
更新日期:2015-02-01 00:00:00
abstract::Extracellular secretion of Serratia marcescens nuclease occurs as a two-step process via a periplasmic intermediate. Unlike other extracellular proteins secreted by gram-negative bacteria by the general secretory pathway, nuclease accumulates in the periplasm in its active form for an unusually long time before its ex...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.3.677-683.1997
更新日期:1997-02-01 00:00:00
abstract::Moorella thermoacetica can grow with H₂ and CO₂, forming acetic acid from 2 CO₂ via the Wood-Ljungdahl pathway. All enzymes involved in this pathway have been characterized to date, except for methylenetetrahydrofolate reductase (MetF). We report here that the M. thermoacetica gene that putatively encodes this enzyme,...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01839-14
更新日期:2014-09-01 00:00:00
abstract::Pakula, Roman (University of Toronto, Toronto, Ontario, Canada). Factors regulating competence in transformation of streptococci. J. Bacteriol. 90:1320-1324. 1965.-The highly transformable group H Streptococcus strain Challis produced an exocellular competence-provoking enzyme capable of converting to the state of com...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.90.5.1320-1324.1965
更新日期:1965-11-01 00:00:00
abstract::Promoters were cloned from the chromosomal DNA of Lactococcus lactis subsp. lactis NP4510 by using promoter-probe vector pGKV210. N-Methyl-N'-nitro-N-nitrosoguanidine-induced mutagenesis of L. lactis-(pBV413), with low-level expression of the cat-86 gene, resulted in enhanced expression. Subcloning and sequencing of t...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.21.6754-6758.1994
更新日期:1994-11-01 00:00:00