Dissociation of Lac messenger ribonucleic acid transcription from translation during recovery from inhibition of protein synthesis.

Abstract:

:Studies were made of the synthesis of Lac messenger ribonucleic acid (mRNA) by Escherichia coli in the absence of protein synthesis and of the coupling of transcription of lac operon to translation. Lac mRNA was not synthesized in the presence of chloramphenicol, and its synthesis steadily decreased during K(+) deprivation and treatment with puromycin. Since under these conditions total mRNA synthesis is not inhibited it is suggested that the control of Lac mRNA is distinct from that which regulates total mRNA synthesis. Lac mRNA synthesized during recovery from K(+) starvation or from chloramphenicol inhibition is not translated into functional enzyme, suggesting translational control over beta-galactosidase synthesis.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Artman M,Ennis HL

doi

10.1128/JB.110.2.652-660.1972

subject

Has Abstract

pub_date

1972-05-01 00:00:00

pages

652-60

issue

2

eissn

0021-9193

issn

1098-5530

journal_volume

110

pub_type

杂志文章
  • Swarming: flexible roaming plans.

    abstract::Movement over an agar surface via swarming motility is subject to formidable challenges not encountered during swimming. Bacteria display a great deal of flexibility in coping with these challenges, which include attracting water to the surface, overcoming frictional forces, and reducing surface tension. Bacteria that...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章,评审

    doi:10.1128/JB.02063-12

    authors: Partridge JD,Harshey RM

    更新日期:2013-03-01 00:00:00

  • GENETIC TRANSFER OF STREPTOMYCIN AND AMINOPROPANOLSTREPTOMYCIN RESISTANCE.

    abstract::von Graevenitz, Alexander (Yale University, New Haven, Conn.). Genetic transfer of streptomycin and aminopropanolstreptomycin resistance. J. Bacteriol. 88:960-964. 1964.-Transduction and conjugation experiments with Staphylococcus aureus and Escherichia coli suggest that resistance to streptomycin (S) and aminopropano...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.88.4.960-964.1964

    authors: VONGRAEVENITZ A

    更新日期:1964-10-01 00:00:00

  • Transcriptional response of Pasteurella multocida to nutrient limitation.

    abstract::Bacteria often encounter environments where nutrient availability is limited, and they must adapt accordingly. To identify Pasteurella multocida genes that are differentially expressed during nutrient limitation, we utilized whole-genome microarrays to compare levels of gene expression during growth in rich and minima...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.13.3734-3739.2002

    authors: Paustian ML,May BJ,Kapur V

    更新日期:2002-07-01 00:00:00

  • Yeast-phase cell cycle of the polymorphic fungus Wangiella dermatitidis.

    abstract::The yeast-phase cell cycle of Wangiella dermatitidis was studied using flow microfluorimetry and the deoxyribonucleic acid (DNA) synthesis inhibitor hydroxyurea (HU). Exposure of exponential-phase yeastlike cells to 0.1 M HU for 3 to 6 h resulted in the arrest of the cells in DNA synthesis and produced a nearly homoge...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.144.2.721-731.1980

    authors: Roberts RL,Szaniszlo PJ

    更新日期:1980-11-01 00:00:00

  • Chemotaxis toward amino acids in Escherichia coli.

    abstract::Escherichia coli cells are shown to be attracted to the l-amino acids alanine, asparagine, aspartate, cysteine, glutamate, glycine, methionine, serine, and threonine, but not to arginine, cystine, glutamine, histidine, isoleucine, leucine, lysine, phenylalanine, tryptophan, tyrosine, or valine. Bacteria grown in a pro...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.112.1.315-326.1972

    authors: Mesibov R,Adler J

    更新日期:1972-10-01 00:00:00

  • Visualization of Pseudomonas aeruginosa O antigens by using a protein A-dextran-colloidal gold conjugate with both immunoglobulin G and immunoglobulin M monoclonal antibodies.

    abstract::Two lipopolysaccharide O-antigen-specific monoclonal antibodies, MA1-8 (an immunoglobulin G1 [IgG1]) and MF15-4 (an IgM), were used to localize the O antigen of the lipopolysaccharide of Pseudomonas aeruginosa PAO1. A protein A-dextran-gold conjugate with an average particle diameter of 12.5 nm was used to label bacte...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.8.3531-3538.1987

    authors: Lam JS,Lam MY,MacDonald LA,Hancock RE

    更新日期:1987-08-01 00:00:00

  • Molecular characterization of the hemolysin determinant of Serratia marcescens.

    abstract::The nucleotide sequence of a 7.3-kilobase-pair fragment of DNA encoding a hemolytic activity from Serratia marcescens was determined. Two large open reading frames were identified, designated shlA (Serratia hemolysin) and shlB, capable of encoding polypeptides of 165, 056 and 61,897 molecular weight, respectively. Bot...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.7.3177-3188.1988

    authors: Poole K,Schiebel E,Braun V

    更新日期:1988-07-01 00:00:00

  • Appearance of a new species of ribonucleic acid during sporulation in Saccharomyces cerevisiae.

    abstract::In the course of study on ribonucleic acid (RNA) metabolism during sporulation in Saccharomyces cerevisiae, a new species of RNA (20S) was observed in sporulating cells by polyacrylamide gel electrophoresis. The relative content of this RNA to total RNA increased linearly early in sporulation. Labeled adenine was pref...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.105.3.826-830.1971

    authors: Kadowaki K,Halvorson HO

    更新日期:1971-03-01 00:00:00

  • The Last r Locus Unveiled: T4 RIII Is a Cytoplasmic Antiholin.

    abstract:UNLABELLED:The latent period of phage T4, normally ∼25 min, can be extended indefinitely if the infected cell is superinfected after 5 min. This phenomenon, designated lysis inhibition (LIN), was first described in the 1940s and is genetically defined by mutations in diverse T4 r genes. RI, the main effector of LIN, ha...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00294-16

    authors: Chen Y,Young R

    更新日期:2016-08-25 00:00:00

  • Bacteriocin protein BacL1 of Enterococcus faecalis targets cell division loci and specifically recognizes L-Ala2-cross-bridged peptidoglycan.

    abstract::Bacteriocin 41 (Bac41) is produced from clinical isolates of Enterococcus faecalis and consists of two extracellular proteins, BacL1 and BacA. We previously reported that BacL1 protein (595 amino acids, 64.5 kDa) is a bacteriolytic peptidoglycan D-isoglutamyl-L-lysine endopeptidase that induces cell lysis of E. faecal...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.02203-14

    authors: Kurushima J,Nakane D,Nishizaka T,Tomita H

    更新日期:2015-01-01 00:00:00

  • Isoleucine auxotrophy as a consequence of a mutationally altered isoleucyl-transfer ribonucleic acid synthetase.

    abstract::Among mutants which require isoleucine, but not valine, for growth, we have found two distinguishable classes. One is defective in the biosynthetic enzyme threonine deaminase (l-threonine hydro-lyase, deaminating, EC 4.2.1.16) and the other has an altered isoleucyl transfer ribonucleic acid (tRNA) synthetase [l-isoleu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.105.2.527-537.1971

    authors: Iaccarino M,Berg P

    更新日期:1971-02-01 00:00:00

  • Constricted flux through the branched-chain amino acid biosynthetic enzyme acetolactate synthase triggers elevated expression of genes regulated by rpoS and internal acidification.

    abstract::The first common enzyme of isoleucine and valine biosynthesis, acetolactate synthase (ALS), is specifically inhibited by the herbicide sulfometuron methyl (SM). To further understand the physiological consequences of flux alterations at this point in metabolism, Escherichia coli genes whose expression was induced by p...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.4.785-792.1998

    authors: Van Dyk TK,Ayers BL,Morgan RW,Larossa RA

    更新日期:1998-02-01 00:00:00

  • Comprehensive Spatial Analysis of the Borrelia burgdorferi Lipoproteome Reveals a Compartmentalization Bias toward the Bacterial Surface.

    abstract::The Lyme disease spirochete Borrelia burgdorferi is unique among bacteria in its large number of lipoproteins that are encoded by a small, exceptionally fragmented, and predominantly linear genome. Peripherally anchored in either the inner or outer membrane and facing either the periplasm or the external environment, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00658-16

    authors: Dowdell AS,Murphy MD,Azodi C,Swanson SK,Florens L,Chen S,Zückert WR

    更新日期:2017-02-28 00:00:00

  • Proton pump coupled to cytochrome c oxidase in the cyanobacterium Anacystis nidulans.

    abstract::Intact spheroplasts of the cyanobacterium Anacystis nidulans were found to oxidize various exogenous c-type cytochromes with concomitant proton extrusion. In the coupled state, H+/e stoichiometries close to 1 were measured, regardless of absolute reaction rates. It is concluded that the proton translocation observed i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.153.1.539-542.1983

    authors: Peschek GA

    更新日期:1983-01-01 00:00:00

  • Fructose 1,6-bisphosphate aldolase activity is essential for synthesis of alginate from glucose by Pseudomonas aeruginosa.

    abstract::We have isolated a mutant of Pseudomonas aeruginosa deficient in fructose 1,6-bisphosphate aldolase activity. This mutant, similar to the mutants deficient in any of the Entner-Doudoroff pathway enzymes, does not allow appreciable alginate formation from glucose and gluconate, but allows alginate synthesis from mannit...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.161.1.458-460.1985

    authors: Banerjee PC,Vanags RI,Chakrabarty AM,Maitra PK

    更新日期:1985-01-01 00:00:00

  • Purification and N-terminal sequence of the alpha subunit of antigen 43, a unique protein complex associated with the outer membrane of Escherichia coli.

    abstract::Antigen 43 has been identified as a unique protein complex in the outer membrane of Escherichia coli. The complex contains two different polypeptides, alpha (Mr, 60,000) and beta (Mr, 53,000), in equal stoichiometry (P. Owen, P. Caffrey, and L.-G. Josefsson, J. Bacteriol. 169:3770-3777, 1987). The alpha subunit was re...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.7.3634-3640.1989

    authors: Caffrey P,Owen P

    更新日期:1989-07-01 00:00:00

  • The TolB protein interacts with the porins of Escherichia coli.

    abstract::TolB is a periplasmic protein of the cell envelope Tol complex. It is partially membrane associated through an interaction with the outer membrane lipoprotein PAL (peptidoglycan-associated lipoprotein), which also belongs to the Tol system. The interaction of TolB with outer membrane porins of Escherichia coli was inv...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.23.7274-7279.1997

    authors: Rigal A,Bouveret E,Lloubes R,Lazdunski C,Benedetti H

    更新日期:1997-12-01 00:00:00

  • The gene for ribosomal protein S21, rpsU, maps close to dnaG at 66.5 min on the Escherichia coli chromosomal linkage map.

    abstract::A series of mutations causing alterations in ribosomal protein S21 was mapped close to dnaG, around 66.5 min on the Escherichia coli chromosomal linkage map. They cotransduced about 95% with this marker. These mutations define gene rpsU, which is very likely the structural gene for protein S21. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.144.2.603-607.1980

    authors: Dabbs ER

    更新日期:1980-11-01 00:00:00

  • Tripartite Regulation of the glpFKD Operon Involved in Glycerol Catabolism by GylR, Crp, and SigF in Mycobacterium smegmatis.

    abstract::The glpD (MSMEG_6761) gene encoding glycerol-3-phosphate dehydrogenase was shown to be crucial for M. smegmatis to utilize glycerol as the sole carbon source. The glpD gene likely forms the glpFKD operon together with glpF and glpK, encoding a glycerol facilitator and glycerol kinase, respectively. The gylR (MSMEG_675...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00511-19

    authors: Bong HJ,Ko EM,Song SY,Ko IJ,Oh JI

    更新日期:2019-11-20 00:00:00

  • Identification of a linked set of genes in Serpulina hyodysenteriae (B204) predicted to encode closely related 39-kilodalton extracytoplasmic proteins.

    abstract::A tandem pair of nearly identical genes from Serpulina hyodysenteriae (B204) were cloned and sequenced. The full open reading frame of one gene and the partial open reading frame of the neighboring gene appear to encode secreted proteins which are homologous to, yet distinct from, the 39-kDa extracytoplasmic protein p...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.2.444-448.1998

    authors: Gabe JD,Dragon E,Chang RJ,McCaman MT

    更新日期:1998-01-01 00:00:00

  • Contribution of σ70 and σN Factors to Expression of Class II pilE in Neisseria meningitidis.

    abstract::Neisseria meningitidis expresses multicomponent organelles called type four pili (Tfp), which are key virulence factors required for attachment to human cells during carriage and disease. Pilin (PilE) is the main component of Tfp, and N. meningitidis isolates either have a class I pilE locus and express pilins that un...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00170-19

    authors: Lobanovska M,Tang CM,Exley RM

    更新日期:2019-09-20 00:00:00

  • Envelope-associated folded chromosomes for Escherichia coli: variations under different physiological conditions.

    abstract::The folded chromosome of Escherichia coli has been investigated under various lysis and physiological conditions. A new gradient system was devised that allows excellent separation between unlysed cells and envelope-associated and envelope-free chromosomes. Isotope incorporation experiments showed that the fraction of...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.127.2.904-916.1976

    authors: Korch C,Ovrebo S,Kleppe K

    更新日期:1976-08-01 00:00:00

  • Phospholipase C regulatory mutation of Pseudomonas aeruginosa that results in constitutive synthesis of several phosphate-repressible proteins.

    abstract::We describe here a new mutant of Pseudomonas aeruginosa PAO, strain D10C (genotype plcB), which produces phospholipase C and alkaline phosphatase constitutively. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the extracellular proteins produced by this mutant in high- and low-Pi media revealed that the m...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.150.3.1221-1226.1982

    authors: Gray GL,Berka RM,Vasil ML

    更新日期:1982-06-01 00:00:00

  • Mutant analysis reveals a specific requirement for protein P30 in Mycoplasma pneumoniae gliding motility.

    abstract::The cell-wall-less prokaryote Mycoplasma pneumoniae, long considered among the smallest and simplest cells capable of self-replication, has a distinct cellular polarity characterized by the presence of a differentiated terminal organelle which functions in adherence to human respiratory epithelium, gliding motility, a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.18.6281-6289.2005

    authors: Hasselbring BM,Jordan JL,Krause DC

    更新日期:2005-09-01 00:00:00

  • Insights into plant cell wall degradation from the genome sequence of the soil bacterium Cellvibrio japonicus.

    abstract::The plant cell wall, which consists of a highly complex array of interconnecting polysaccharides, is the most abundant source of organic carbon in the biosphere. Microorganisms that degrade the plant cell wall synthesize an extensive portfolio of hydrolytic enzymes that display highly complex molecular architectures. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01701-07

    authors: DeBoy RT,Mongodin EF,Fouts DE,Tailford LE,Khouri H,Emerson JB,Mohamoud Y,Watkins K,Henrissat B,Gilbert HJ,Nelson KE

    更新日期:2008-08-01 00:00:00

  • Recruitment of the ParG segregation protein to different affinity DNA sites.

    abstract::The segrosome is the nucleoprotein complex that mediates accurate plasmid segregation. In addition to its multifunctional role in segrosome assembly, the ParG protein of multiresistance plasmid TP228 is a transcriptional repressor of the parFG partition genes. ParG is a homodimeric DNA binding protein, with C-terminal...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01630-08

    authors: Zampini M,Derome A,Bailey SE,Barillà D,Hayes F

    更新日期:2009-06-01 00:00:00

  • Cell wall turnover in batch and chemostat cultures of Bacillus subtilis.

    abstract::Wall turnover was studied in Bacillus subtilis. The loss of radioactively labeled wall polymers was followed during exponential growth in batch and chemostat cultures. Turnover kinetics were identical under all growth conditions; pulse-labeled wall material was lost with first-order kinetics, but only after exponentia...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.145.1.50-60.1981

    authors: De Boer WR,Kruyssen FJ,Wouters JT

    更新日期:1981-01-01 00:00:00

  • Extracellular hemolysins of aerobic sporogenic bacilli.

    abstract::Forty-five strains, representing 18 species of the genus Bacillus, were surveyed for production of hemolysin against rabbit erythrocytes. Broth cultures of B. cereus, B. alvei, and B. laterosporus contained lysins that closely resembled streptolysin O. B. subtilis and a single strain of B. cereus may produce lysins ha...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.93.5.1541-1543.1967

    authors: Bernheimer AW,Grushoff P

    更新日期:1967-05-01 00:00:00

  • Identification of formate dehydrogenase-specific mRNA species and nucleotide sequence of the fdhC gene of Methanobacterium formicicum.

    abstract::The overlapping fdhA and fdhB genes of Methanobacterium formicicum, which encode the alpha and beta subunits, respectively, of formate dehydrogenase, were cotranscribed as part of an approximately 4.5-kb transcript. An additional gene (fdhC) upstream of fdhA was cotranscribed with fdhA and fdhB. The deduced amino acid...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.15.4997-5004.1992

    authors: White WB,Ferry JG

    更新日期:1992-08-01 00:00:00

  • Role of adaptor TrfA and ClpPC in controlling levels of SsrA-tagged proteins and antitoxins in Staphylococcus aureus.

    abstract::Staphylococcus aureus responds to changing extracellular environments in part by adjusting its proteome through alterations of transcriptional priorities and selective degradation of the preexisting pool of proteins. In Bacillus subtilis, the proteolytic adaptor protein MecA has been shown to play a role in assisting ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.02222-14

    authors: Donegan NP,Marvin JS,Cheung AL

    更新日期:2014-12-01 00:00:00