Propanediol utilization genes (pdu) of Salmonella typhimurium: three genes for the propanediol dehydratase.

Abstract:

:The propanediol utilization (pdu) operon of Salmonella typhimurium encodes proteins required for the catabolism of propanediol, including a coenzyme B12-dependent propanediol dehydratase. A clone that expresses propanediol dehydratase activity was isolated from a Salmonella genomic library. DNA sequence analysis showed that the clone included part of the pduF gene, the pduABCDE genes, and a long partial open reading frame (ORF1). The clone included 3.9 kbp of pdu DNA which had not been previously sequenced. Complementation and expression studies with subclones constructed via PCR showed that three genes (pduCDE) are necessary and sufficient for propanediol dehydratase activity. The function of ORF1 was not determined. Analyses showed that the S. typhimurium propanediol dehydratase was related to coenzyme B12-dependent glycerol dehydratases from Citrobacter freundii and Klebsiella pneumoniae. Unexpectedly, the S. typhimurium propanediol dehydratase was found to be 98% identical in amino acid sequence to the Klebsiella oxytoca propanediol dehydratase; this is a much higher identity than expected, given the relationship between these organisms. DNA sequence analyses also supported previous studies indicating that the pdu operon was inherited along with the adjacent cobalamin biosynthesis operon by a single horizontal gene transfer.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Bobik TA,Xu Y,Jeter RM,Otto KE,Roth JR

doi

10.1128/jb.179.21.6633-6639.1997

subject

Has Abstract

pub_date

1997-11-01 00:00:00

pages

6633-9

issue

21

eissn

0021-9193

issn

1098-5530

journal_volume

179

pub_type

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