Genetic characterization of the temperature-sensitive and suppression phenotypes of Escherichia coli mutant N4316.

abstract：:In Staphylococcus aureus, the transport of dicarboxylates is mediated in part by the Na+-linked carrier protein SdcS. This transporter is a member of the divalent-anion/Na+ symporter (DASS) family, a group that includes the mammalian Na+/dicarboxylate cotransporters NaDC1 and NaDC3. In earlier work, we cloned and expr...

journal_title：Journal of bacteriology

authors： Hall JA,Pajor AM

更新日期：2007-02-01 00:00:00

abstract：:Whole cells of Bacillus subtilis transported d-alanine and l-alanine by two different systems. The high-affinity system (K(m) of 1 muM and V(max) of 0.6 to 0.8 nmol/min per mg of protein) was specific for the two stereoisomers of alanine. The low-affinity system (K(m) of 10 muM for l-alanine and 20 muM for d-alanine a...

journal_title：Journal of bacteriology

authors： Clark VL,Young FE

更新日期：1974-12-01 00:00:00

abstract：:At least six phenotypically distinct classes of mutants of Escherichia coli which require serine or pyridoxine or both can be isolated. Three of the six classes lack 3-phosphoserine-2-oxoglutarate aminotransferase. One of these classes contains WG5, a mutant previously characterized as containing the pdxF5 allele. The...

journal_title：Journal of bacteriology

authors： Dempsey WB,Ito H

更新日期：1970-11-01 00:00:00

abstract：:We report a draft sequence of the genome of Gordonia neofelifaecis NRRL B-59395, a cholesterol-degrading actinomycete isolated from fresh feces of a clouded leopard (Neofelis nebulosa). As predicted, the reported genome contains several gene clusters for cholesterol degradation. This is the second available genome seq...

journal_title：Journal of bacteriology

authors： Ge F,Li W,Chen G,Liu Y,Zhang G,Yong B,Wang Q,Wang N,Huang Z,Li W,Wang J,Wu C,Xie Q,Liu G

更新日期：2011-09-01 00:00:00

abstract：:We have identified a gene encoding a putative membrane protein homologous to the major facilitator superfamily, mapping upstream of the lacS gene in Sulfolobus solfataricus. Permeases from this family mediate secondary transport and are widely distributed among eubacteria and eukaryotes; the finding of an archaeal mem...

journal_title：Journal of bacteriology

authors： Prisco A,Moracci M,Rossi M,Ciaramella M

更新日期：1995-03-01 00:00:00

abstract：:The nucleoids in Mycoplasma capricolum cells were visualized by phase-combined fluorescence microscopy of DAPI (4', 6-diamidino-2-phenylindole)-stained cells. Most growing cells in a rich medium had one or two nucleoids in a cell, and no anucleate cells were found. The nucleoids were positioned in the center in mononu...

journal_title：Journal of bacteriology

authors： Seto S,Miyata M

更新日期：1999-10-01 00:00:00

abstract：:t is the holin gene for coliphage T4, encoding a 218-amino-acid (aa) protein essential for the inner membrane hole formation that initiates lysis and terminates the phage infection cycle. T is predicted to be an integral membrane protein that adopts an N(in)-C(out) topology with a single transmembrane domain (TMD). Th...

journal_title：Journal of bacteriology

authors： Moussa SH,Lawler JL,Young R

更新日期：2014-06-01 00:00:00

abstract：:Type VI secretion systems (T6SS) are bacteriophage-derived macromolecular machines responsible for the release of at least two proteins in the milieu, which are thought to form an extracellular appendage. Although several T6SS have been shown to be involved in the virulence of animal and plant pathogens, clusters enco...

journal_title：Journal of bacteriology

authors： Bernard CS,Brunet YR,Gavioli M,Lloubès R,Cascales E

更新日期：2011-05-01 00:00:00

abstract：:The Escherichia coli promoter p(BAD), under the control of the AraC protein, drives the expression of mRNA encoding the AraB, AraA, and AraD gene products of the arabinose operon. The binding site of AraC at p(BAD) overlaps the RNA polymerase -35 recognition region by 4 bases, leaving 2 bases of the region not contact...

journal_title：Journal of bacteriology

authors： Dhiman A,Schleif R

更新日期：2000-09-01 00:00:00

abstract：:The Escherichia coli htrD gene was originally isolated during a search for new genes required for growth at high temperature. Insertional inactivation of htrD leads to a pleiotropic phenotype characterized by temperature-sensitive growth in rich medium, H2O2 sensitivity, and sensitivity to cysteine. The htrD gene was ...

journal_title：Journal of bacteriology

authors： Delaney JM,Wall D,Georgopoulos C

更新日期：1993-01-01 00:00:00

abstract：:The generalized transducing phage T4GT7 grew well on recA strains of Escherichia coli and transduced recA into F- and Hfr strains of E. coli at high frequency. ...

journal_title：Journal of bacteriology

authors： Plakidou S,Moffat KG,Salmond GP,Mackinnon G

更新日期：1984-09-01 00:00:00

abstract：:The general stress regulon of Bacillus subtilis comprises approximately 200 genes and is under the control of the alternative sigma factor σ(B). The activation of σ(B) occurs in response to multiple physical stress stimuli as well as energy starvation conditions. The expression of the general stress proteins provides ...

journal_title：Journal of bacteriology

authors： Reder A,Höper D,Gerth U,Hecker M

更新日期：2012-07-01 00:00:00

abstract：:Addition of casein hydrolysate to suspensions of washed, nonpigmented, nonproliferating Serratia marcescens incubating at 27 C induced biosynthesis of prodigiosin. Four amino acids of casein hydrolysate, dl-aspartic acid, l-glutamic acid, l-proline, and l-alanine caused formation of pigment when added individually. dl...

journal_title：Journal of bacteriology

authors： Williams RP,Gott CL,Qadri SM

更新日期：1971-05-01 00:00:00

abstract：:Rhizobium leguminosarum strain VF39SM contains two plasmids that have previously been shown to be self-transmissible by conjugation. One of these plasmids, pRleVF39b, is shown in this study to carry a set of plasmid transfer genes that differs significantly from conjugation systems previously studied in the rhizobia b...

journal_title：Journal of bacteriology

authors： Ding H,Yip CB,Hynes MF

更新日期：2013-01-01 00:00:00

abstract：:The vir-type IV secretion system of Agrobacterium is assembled from 12 proteins encoded by the virB operon and virD4. VirB1 is one of the least-studied proteins encoded by the virB operon. Its N terminus is a lytic transglycosylase. The C-terminal third of the protein, VirB1*, is cleaved from VirB1 and secreted to the...

journal_title：Journal of bacteriology

authors： Zupan J,Hackworth CA,Aguilar J,Ward D,Zambryski P

更新日期：2007-09-01 00:00:00

abstract：:We report on the characterization of three new transcription units expressed during sporulation in Bacillus subtilis. Two of the units, cse15 and cse60, were mapped at about 123 degrees and 62 degrees on the genetic map, respectively. Their transcription commenced around h 2 of sporulation and showed an absolute requi...

journal_title：Journal of bacteriology

authors： Henriques AO,Bryan EM,Beall BW,Moran CP Jr

更新日期：1997-01-01 00:00:00

abstract：:This study examined the catabolism of xanthone by an Arthrobacter sp. (strain GFB100) capable of growth on xanthone as its main source of carbon and energy. An early catabolic intermediate was 3,4-dihydroxyxanthone. This compound was isolated from the growth medium of a mutant strain of the Arthrobacter sp. which lack...

journal_title：Journal of bacteriology

authors： Tomasek PH,Crawford RL

更新日期：1986-09-01 00:00:00

abstract：:A three-dimensional tracking microscope was used to quantify the effects of temperature (50 to 80 degrees C) and pH (2 to 4) on the motility of Sulfolobus acidocaldarius, a thermoacidophilic archaeon. Swimming speed and run time increased with temperature but remained relatively unchanged with increasing pH. These res...

journal_title：Journal of bacteriology

authors： Lewus P,Ford RM

更新日期：1999-07-01 00:00:00

abstract：:Alginate-producing (mucoid) strains of Pseudomonas aeruginosa possess a 54-kDa outer membrane (OM) protein (AlgE) which is missing in nonmucoid bacteria. The coding region of the algE gene from mucoid P. aeruginosa CF3/M1 was subcloned in the expression vector pT7-7 and expressed in Escherichia coli. The level of expr...

journal_title：Journal of bacteriology

authors： Rehm BH,Boheim G,Tommassen J,Winkler UK

更新日期：1994-09-01 00:00:00

abstract：:Isolation of highly purified membrane fractions from phototrophically grown Rhodospirillum rubrum was achieved by velocity and isopyknic sedimentation under carefully controlled ionic conditions. Bacteriochlorophyll-rich and succinic dehydrogenase-rich chromatophores that were essentially devoid of contamination by no...

journal_title：Journal of bacteriology

authors： Collins ML,Niederman RA

更新日期：1976-06-01 00:00:00

abstract：:Legionella pneumophila expresses two catalase-peroxidase enzymes that exhibit strong peroxidatic but weak catalatic activities, suggesting that other enzymes participate in decomposition of hydrogen peroxide (H2O2). Comparative genomics revealed that L. pneumophila and its close relative Coxiella burnetii each contain...

journal_title：Journal of bacteriology

authors： LeBlanc JJ,Davidson RJ,Hoffman PS

更新日期：2006-09-01 00:00:00

abstract：:RsbT is an essential component of the pathway that activates the Bacillus subtilis sigma(B) transcription factor in response to physical stress. rsbT is located within an operon that includes the genes for its principal negative regulator (RsbS) and the stress pathway component that it activates (RsbU), as immediate u...

journal_title：Journal of bacteriology

authors： Zhang S,Reeves A,Woodbury RL,Haldenwang WG

更新日期：2005-12-01 00:00:00

abstract：:An apparent operator-constitutive mutation was discovered in the repressible acid phosphatase system in Saccharomyces cerevisiae. The site of mutation, designated PHOO, was found to be closely linked to the phoD locus. The mutant allele, PHOO, was semidominant over the wild-type allele and effective for the expression...

journal_title：Journal of bacteriology

authors： Toh-E A,Oshima Y

更新日期：1974-11-01 00:00:00

abstract：:Integral membrane proteins from over 20 ubiquitous families of channels, secondary carriers, and primary active transporters were analyzed for average size differences between homologues from the three domains of life: Bacteria, Archaea, and Eucarya. The results showed that while eucaryotic homologues are consistently...

journal_title：Journal of bacteriology

authors： Chung YJ,Krueger C,Metzgar D,Saier MH Jr

更新日期：2001-02-01 00:00:00

abstract：:The ability of different metal ions to promote transformation of Pseudomonas aeruginosa by deoxyribonucleic acid of the plasmid RP1 was examined. CaCl2, MgCl2, and MnCl2 were found to promote such transformation, although at different frequencies and with the optimum response at different concentrations. Only MgCl2 pr...

journal_title：Journal of bacteriology

authors： Mercer AA,Loutit JS

更新日期：1979-10-01 00:00:00

abstract：:Previously, we characterized a pathway necessary for the processing of NAD+ and for uptake of nicotinamide riboside (NR) in Haemophilus influenzae. Here we report on the role of NadR, which is essential for NAD+ utilization in this organism. Different NadR variants with a deleted ribonucleotide kinase domain or with a...

journal_title：Journal of bacteriology

authors： Merdanovic M,Sauer E,Reidl J

更新日期：2005-07-01 00:00:00

abstract：:A strain of Escherichia coli with a mutation in the promoter proximal gene ( uncI ) of the unc operon has been constructed by using a new gene replacement method. The mutation is a deletion of a defined sequence of 196 base pairs. It was constructed by homologous integration and segregation of a ColE1-derived recombin...

journal_title：Journal of bacteriology

authors： Gay NJ

更新日期：1984-06-01 00:00:00

abstract：:Daniel, John W. (University of Wisconsin, Madison), and Karlee Babcock. Methionine metabolism of the myxomycete Physarum polycephalum. J. Bacteriol. 92:1028-1035. 1966.-Previous studies have shown that Physarum polycephalum requires exogenous methionine for growth, but not cysteine, folic acid, or vitamin B(12). Methi...

journal_title：Journal of bacteriology

authors： Daniel JW,Babcock K

更新日期：1966-10-01 00:00:00

abstract：:Hottle, G. A. (Naval Biological Laboratory, University of California, Berkeley), and D. N. Wright. Growth and survival of Mycoplasma neurolyticum in liquid media. J. Bacteriol. 91:1834-1839. 1966.-Maximal growth of Mycoplasma neurolyticum (between 10(8) and 10(9) colony-forming units per ml) was obtained after 3 days ...

journal_title：Journal of bacteriology

authors： Hottle GA,Wright DN

更新日期：1966-05-01 00:00:00

abstract：:A sulfonic acid found to be a major constituent of spores of Bacillus subtilis was provisionally identified as 3-l-sulfolactic acid. This compound was completely absent from vegetative cells during growth, but large amounts accumulated in sporulating cells just before the development of refractile spores. Essentially ...

journal_title：Journal of bacteriology

authors： Bonsen PP,Spudich JA,Nelson DL,Kornberg A

更新日期：1969-04-01 00:00:00