Abstract:
:The genes for the complete pathways for two polycyclic aromatic polyketides of the angucyclinone class have been cloned and heterologously expressed. Genomic DNAs of Streptomyces rimosus NRRL 3016 and Streptomyces strain WP 4669 were partially digested with MboI, and libraries (ca. 40-kb fragments) in Escherichia coli XL1-Blue MR were prepared with the cosmid vector pOJ446. Hybridization with the actI probe from the actinorhodin polyketide synthase genes identified two clusters of polyketide genes from each organism. After transfer of the four clusters to Streptomyces lividans TK24, expression of one cluster from each organism was established through the identification of pathway-specific products by high-performance liquid chromatography with photodiode array detection. Peaks were identified from the S. rimosus cluster (pksRIM-1) for tetrangulol, tetrangomycin, and fridamycin E. Peaks were identified from the WP 4669 cluster (pksWP-2) for tetrangulol, 19-hydroxytetrangulol, 8-O-methyltetrangulol, 19-hydroxy-8-O-methyltetrangulol, and PD 116740. Structures were confirmed by 1H nuclear magnetic resonance spectroscopy and high-resolution mass spectrometry.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Hong ST,Carney JR,Gould SJdoi
10.1128/jb.179.2.470-476.1997subject
Has Abstractpub_date
1997-01-01 00:00:00pages
470-6issue
2eissn
0021-9193issn
1098-5530journal_volume
179pub_type
杂志文章abstract::The filamentous cyanobacterium Anabaena fixes nitrogen in specialized cells called heterocysts. The immediate product of fixation, ammonia, is known to be assimilated by addition to glutamate to make glutamine. How fixed nitrogen is transported along the filament to the 10 to 20 vegetative cells that separate heterocy...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01512-12
更新日期:2013-01-01 00:00:00
abstract::We have previously identified three sites, named sbi, ihfA, and sbyA, specifically recognized or bound by the TraI, IHF, and TraY proteins, respectively; these sites are involved in nicking at the origin of transfer, oriT, of plasmid R100. In the region next to these sites, there exists the sbm region, which consists ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.15.4350-4355.1995
更新日期:1995-08-01 00:00:00
abstract::Alcaligenes sp. strain CC1 is able to grow on several alpha-chlorinated aliphatic acids (2-chlorobutyrate, 2-chloropropionate, and chloroacetate), as well as on the beta-chlorinated four-carbon aliphatic acids trans-3-chlorocrotonate, cis-3-chlorocrotonate, and 3-chlorobutyrate as sole carbon and energy sources. Dehal...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.3.1428-1434.1989
更新日期:1989-03-01 00:00:00
abstract::Late log-phase Escherichia coli B/r cells are 1.6 times more sensitive to killing by X rays than are stationary-phase cells when grown in Brain Heart Infusion (BHI) + glucose. The number of single-chain breaks formed per krad is the same for log- and stationary-phase cells. Stationary-phase cells show a somewhat great...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.105.1.127-135.1971
更新日期:1971-01-01 00:00:00
abstract::Dehalococcoides species are key players in the anaerobic transformation of halogenated solvents at contaminated sites. Here, we analyze isotopologue distributions in amino acid pools from peptides of Dehalococcoides strain CBDB1 after incubation with (13)C-labeled acetate or bicarbonate as a carbon source. The resulti...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00049-12
更新日期:2012-08-01 00:00:00
abstract::Mutants of Escherichia coli capable of growing in the presence of 10 microgram of mecillinam per ml were selected after intensive mutagenesis. Of these mutants, 1.4% formed normal, rod-shaped cells at 30 degrees C but grew as spherical cells at 42 degrees C. The phenotype of one of these rod(Ts) mutants was 88% cotran...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.136.3.1143-1158.1978
更新日期:1978-12-01 00:00:00
abstract::An in vitro constructed plasmid, pVH15, consisting of the entire genome of the plasmid ColE1, the tryptophan operon of Escherichia coli, and regions of the bacteriophage PHI80pt190, spontaneously gave rise in E. coli to a mini-ColE1 plasmid consisting of approximately one-half of the ColE1 genome and a small segment o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.126.1.447-453.1976
更新日期:1976-04-01 00:00:00
abstract::Protein and ribonucleic acid (RNA) syntheses were measured in both lethally injured and thermally damaged viable cells after heating at lethal temperatures. Immediately after heating, cells were incubated in growth media containing either [14C]leucine or [3H]uracil. The labeled cells were subsequently treated with pen...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.138.2.492-498.1979
更新日期:1979-05-01 00:00:00
abstract::The degradation of synthetic compounds requires bacteria to recruit and adapt enzymes from pathways for naturally occurring compounds. Previous work defined the steps in 2,4-dinitrotoluene (2,4-DNT) metabolism through the ring fission reaction. The results presented here characterize subsequent steps in the pathway th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.15.4219-4232.2002
更新日期:2002-08-01 00:00:00
abstract::From several presumably pure cultures of Thiobacillus ferrooxidans, we isolated a pair of stable phenotypes. One was a strict autotroph utilizing sulfur or ferrous iron as the energy source and unable to utilize glucose; the other phenotype was an acidophilic obligate heterotroph capable of utilizing glucose but not s...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.143.1.448-454.1980
更新日期:1980-07-01 00:00:00
abstract::MotA contains a conserved C-terminal cluster of negatively charged residues, and MotB contains a conserved N-terminal cluster of positively charged residues. Charge-altering mutations affecting these residues impair motility but do not diminish Mot protein levels. The motility defects are reversed by second-site mutat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00407-08
更新日期:2008-08-01 00:00:00
abstract::Pizer, Lewis I. (University of Pennsylvania, Philadelphia). Glycine synthesis and metabolism in Escherichia coli. J. Bacteriol. 89:1145-1150. 1965.-A correlation was demonstrated between a nutritional requirement that can only be satisfied by glycine and the absence of the enzymatic capacity to interconvert l-serine a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.89.4.1145-1150.1965
更新日期:1965-04-01 00:00:00
abstract::The current work by Jain et al. (S. Jain, A. M. Chang, M. Singh, J. S. McLean, et al., J Bacteriol 201:e00683-18, 2019, https://doi.org/10.1128/JB.00683-18) reports the cloning of the lipid A deacylase gene of Porphyromonas gingivalis and the phenotypic characterization of the enzyme. Attempts to clone the gene and th...
journal_title:Journal of bacteriology
pub_type: 评论,杂志文章
doi:10.1128/JB.00146-19
更新日期:2019-05-08 00:00:00
abstract::The factors influencing the digestion of pebble-milled cellulose by enzymes were studied by using several strains of Ruminococcus albus including a mutant characterized by a more eccentric location of its colony in the clearing produced by digestion of the cellulose in the thin layer lining the wall of a culture tube....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.114.2.729-737.1973
更新日期:1973-05-01 00:00:00
abstract::We report here the structure of a previously uncharacterized phospholipid in the radiation-resistant bacterium Deinococcus radiodurans. This phospholipid, designated lipid 4, was shown by chemical analysis, HF hydrolysis, and nuclear magnetic resonance spectroscopy to be phosphatidylglyceroylalkylamine. Lipid 4 thus c...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.2.457-462.1991
更新日期:1991-01-01 00:00:00
abstract::Caulobacter crescentus trp mutants were identified from a collection of auxotrophs. Precursor feeding experiments, accumulation studies, and complementation experiments resulted in the identification of six genes corresponding to trpA, trpB, trpC, trpD, trpE, and trpF. Genetic mapping experiments demonstrated that the...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.160.1.279-287.1984
更新日期:1984-10-01 00:00:00
abstract::The composition of the Pseudomonas solanacearum lipolysaccharide (LPS) was found to be similar to that described for the LPS of enterobacteria. The lipid A contained fatty acids and glucosamine in a molar ratio of 5:2. The LPS fraction contained 2-keto-3-deoxyoctulosonic acid, L-glycero-D-mannoheptose, hexoses (glucos...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.162.2.504-509.1985
更新日期:1985-05-01 00:00:00
abstract::The virulence factors of the cariogenic bacterium Streptococcus sobrinus have been difficult to assess because of a lack of tools for the genetic manipulation of this organism. The construction of an Escherichia coli-Streptococcus shuttle vector, pDL289, that can be mobilized into S. sobrinus by the conjugative plasmi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.17.5028-5034.1995
更新日期:1995-09-01 00:00:00
abstract::The broad-host-range plasmid RK2 is capable of replication and stable maintenance within a wide range of gram-negative bacterial hosts. It encodes the essential replication initiation protein TrfA, which binds to the host initiation protein, DnaA, at the plasmid origin of replication (oriV). There are two versions of ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00231-06
更新日期:2006-08-01 00:00:00
abstract::Respiration-deficient mutants (Rho-, petite) of Saccharomyces carlsbergensis were obtained by treatment with trypaflavin (euflavine). Dried cells of these mutants phosphorylated mononucleotides to their triphosphates and further formed not only cytidine 5'-diphosphate-choline, but also sugar nucleotides, such as uridi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.125.2.744-746.1976
更新日期:1976-02-01 00:00:00
abstract::The umuDC gene products, whose expression is induced by DNA-damaging treatments, have been extensively characterized for their role in SOS mutagenesis. We have recently presented evidence that supports a role for the umuDC gene products in the regulation of growth after DNA damage in exponentially growing cells, analo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.4.1127-1135.2000
更新日期:2000-02-01 00:00:00
abstract::In Shigella flexneri, the polysaccharide copolymerase (PCP) protein Wzz(SF) confers a modal length of 10 to 17 repeat units (RUs) to the O-antigen (Oag) component of lipopolysaccharide (LPS). PCPs form oligomeric structures believed to be related to their function. To identify functionally important regions within Wzz...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01134-09
更新日期:2010-07-01 00:00:00
abstract::The M-band technique was used to assess the number of attachment points of DNA to the cell membrane of Streptococcus faecalis grown at three different rates. Cells were X irradiated in liquid nitrogen and then analyzed simultaneously for the introduction of double-strand breaks into the chromosome and the degree of re...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:
更新日期:1982-10-01 00:00:00
abstract::The control of men gene expression during growth and sporulation of Bacillus subtilis was examined at the transcriptional level. Two different approaches were used. (i) Steady-state levels of men-specific mRNA were measured directly. (ii) A men'-lacZ gene fusion was constructed. In both cases, it was observed that men...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.6.2742-2748.1988
更新日期:1988-06-01 00:00:00
abstract::The exosporium of Bacillus anthracis is comprised of two distinct layers: a basal layer and a hair-like nap that covers the basal layer. The hair-like nap contains the glycoproteins BclA and, most likely, BclB. BclA and BclB are directed to assemble into the exosporium by motifs in their N-terminal domains. Here, we i...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.05658-11
更新日期:2011-10-01 00:00:00
abstract:UNLABELLED:Resistance to methicillin and other β-lactam antibiotics in staphylococci is due to mecA, which is carried on a genomic island, staphylococcal cassette chromosome mec (SCCmec). The chromosomal excision and integration of SCCmec are mediated by the site-specific recombinase CcrAB or CcrC, encoded within this ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00078-15
更新日期:2015-09-01 00:00:00
abstract::The interaction of 12 phage strains with bacilli, protoplasts, and L forms of Bacillus subtilis 168 and with eight of its mutants and two of its lysogens is described qualitatively and quantitatively. After removal of the cell wall from B. subtilis 168, 11 of the 12 phage strains can still adsorb to the protoplasts, n...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.124.1.445-448.1975
更新日期:1975-10-01 00:00:00
abstract::The level of the vitamin B12 transport protein BtuB in the outer membrane of Escherichia coli is strongly reduced by growth in the presence of cobalamins. Previous analyses of regulatory mutants and of btuB-lacZ fusions indicated that the primary site of btuB gene regulation was at the translational level, and this re...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.24.6719-6728.1998
更新日期:1998-12-01 00:00:00
abstract::The glnA mRNA, encoding glutamine synthetase, is differentially accumulated in the cyanobacterium Synechococcus sp. strain PCC 7942 in media containing different nitrogen sources. With the different nitrogen compounds, transcription of glnA initiated at a single site located -146 nucleotides upstream of the translatio...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.23.7727-7731.1993
更新日期:1993-12-01 00:00:00
abstract::Linker insertion mutagenesis was used to modify the paracrystalline surface layer (S-layer) protein (RsaA) of the gram-negative bacterium Caulobacter crescentus. Eleven unique BamHI linker insertions in the cloned rsaA gene were identified; at the protein level, these linker insertions introduced 4 to 6 amino acids at...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.3.601-611.1997
更新日期:1997-02-01 00:00:00