Overexpression of the MarA positive regulator is sufficient to confer multiple antibiotic resistance in Escherichia coli.

abstract：:Membrane vesicles isolated from competent cultures of Bacillus subtilis 168 bound up to 20 mug of double-stranded deoxyribonucleic acid (DNA) per mg of membrane protein in the presence of ethylenediaminetetraacetate. The formation of the DNA-membrane complex was time, temperature, and pH dependent. Eighty per cent of ...

journal_title：Journal of bacteriology

authors： Joenje H,Konings WN,Venema G

更新日期：1974-09-01 00:00:00

abstract：:Aggregative amoebae of Dictyostelium discoideum, D. mucoroides, D. purpureum, and D. rosarium react chemotactically to cyclic AMP (cAMP). We measured the chemotactic activity of 14 cAMP analogs and found that these four species have a similar sensitivity to chemical modifications of cAMP; this suggests that the cAMP r...

journal_title：Journal of bacteriology

authors： Van Haastert PJ,Jastorff B,Pinas JE,Konijn TM

更新日期：1982-01-01 00:00:00

abstract：:The isolation of auxotrophic mutants of Bacillus licheniformis, a microbe containing constitutive beta-lactamase activity, was found to be facilitated by the addition of clavulanic acid and cefotaxime during enrichment. ...

journal_title：Journal of bacteriology

authors： Fields PI,Schreier HJ,Saha AL,Bernlohr RW

更新日期：1984-08-01 00:00:00

abstract：:Satellite bacteriophage P4 requires the products of the late genes of a helper such as P2 in order to grow lytically. The Escherichia coli rpoA109 mutation, which alters the alpha subunit of RNA polymerase, prevents transcription of the late genes of bacteriophage P2. Suppressor mutations that define the P2 ogr gene o...

journal_title：Journal of bacteriology

authors： Halling C,Sunshine MG,Lane KB,Six EW,Calendar R

更新日期：1990-07-01 00:00:00

abstract：:D-Ribulose 1,5-bisphosphate (RuBP) carboxylase has been purified from the photosynthetic extreme halophile Ectothiorhodospira halophila. Despite a growth requirement for almost saturating sodium chloride in the medium, both crude and homogeneous preparations of RuBP carboxylase obtained from this organism were inhibit...

journal_title：Journal of bacteriology

authors： Tabita FR,McFadden BA

更新日期：1976-06-01 00:00:00

abstract：:Wood, N. P. (A. & M. College of Texas, College Station), and D. J. O'Kane. Formate-pyruvate exchange reaction in Streptococcus faecalis. I. Factor requirement for whole cells. J. Bacteriol. 87:97-103. 1964.-A factor present in plant and animal sources was found necessary for the incorporation of formate-C(14) into pyr...

journal_title：Journal of bacteriology

authors： WOOD NP,O'KANE DJ

更新日期：1964-01-01 00:00:00

abstract：:Insertion of Tn7 in nine independently derived isolates occurred in a single small region of the Caulobacter crescentus chromosome, suggesting that Tn7 insertion occurs at or near a single site. The order of markers in this region of the Caulobacter chromosome was shown to be flaA-argG-Tn7. ...

journal_title：Journal of bacteriology

authors： Ely B

更新日期：1982-08-01 00:00:00

abstract：:We purified active hydrogenase from free-living Rhizobium japonicum by affinity chromatography. The uptake hydrogenase of R. japonicum has been treated previously as an oxygen-sensitive protein. In this purification, however, reducing agents were not added nor was there any attempt to exclude oxygen. In fact, the addi...

journal_title：Journal of bacteriology

authors： Stults LW,Moshiri F,Maier RJ

更新日期：1986-06-01 00:00:00

abstract：:We report the first example of a gene, hmp, encoding a soluble flavohemoglobin in Escherichia coli K-12, which is up-regulated by paraquat in a SoxRS-independent manner. Unlike what is found for other paraquat-inducible genes, high concentrations of paraquat (200 microM) were required to increase the level of hmp expr...

journal_title：Journal of bacteriology

authors： Membrillo-Hernández J,Kim SO,Cook GM,Poole RK

更新日期：1997-05-01 00:00:00

abstract：:RNase D is a 3'-exoribonuclease whose in vitro specificity has suggested that it is involved in the processing of tRNA precursors. Its in vivo role has remained unclear, however, because mutant cells devoid of the enzyme display no defect in growth or tRNA processing. To learn more about the structure and function of ...

journal_title：Journal of bacteriology

authors： Zhang JR,Deutscher MP

更新日期：1988-02-01 00:00:00

abstract：:Growth of Agrobacterium tumefaciens on d-glucuronic acid (GlcUA) or d-galacturonic acid (GalUA) induces formation of hexuronic acid dehydrogenase [d-aldohexuronic acid: nicotinamide adenine dinucleotide (NAD) oxidoreductase]. The dehydrogenase, which irreversibly converts GlcUA or GalUA to the corresponding hexaric ac...

journal_title：Journal of bacteriology

authors： Chang YF,Feingold DS

更新日期：1969-09-01 00:00:00

abstract：:CheY is the response regulator in the signal transduction pathway of bacterial chemotaxis. Position 106 of CheY is occupied by a conserved aromatic residue (tyrosine or phenylalanine) in the response regulator superfamily. A number of substitutions at position 106 have been made and characterized by both behavioral an...

journal_title：Journal of bacteriology

authors： Zhu X,Amsler CD,Volz K,Matsumura P

更新日期：1996-07-01 00:00:00

abstract：:A circular genetic map of Bacillus stearothermophilus NUB36 was constructed by transduction with bacteriophage TP-42C and protoplast fusion. Sixty-four genes were tentatively assigned a cognate Bacillus subtilis gene based on growth response to intermediates or end products of metabolism, cross-feeding, accumulation o...

journal_title：Journal of bacteriology

authors： Vallier H,Welker NE

更新日期：1990-02-01 00:00:00

abstract：:Dworkin, Martin (University of Minnesota, Minneapolis), and Donald J. Niederpruem. Electron transport system in vegetative cells and microcysts of Myxococcus xanthus. J. Bacteriol. 87:316-322. 1964.-Respiration by intact cells of the fruiting myxobacterium Myxococcus xanthus is cyanide-sensitive and can be demonstrate...

journal_title：Journal of bacteriology

authors： DWORKIN M,NIEDERPRUEM DJ

更新日期：1964-02-01 00:00:00

abstract：:Chromatin immunoprecipitation and microarray (ChIP-chip) analysis showed that the nitric oxide (NO)-sensitive repressor NsrR from Escherichia coli binds in vivo to the promoters of the tynA and feaB genes. These genes encode the first two enzymes of a pathway that is required for the catabolism of phenylethylamine (PE...

journal_title：Journal of bacteriology

authors： Rankin LD,Bodenmiller DM,Partridge JD,Nishino SF,Spain JC,Spiro S

更新日期：2008-09-01 00:00:00

abstract：:When Methanobacterium thermoautotrophicum cells were incubated in 50 mM potassium phosphate buffer (pH 7.0) containing 1 M sucrose and autolysate from Methanobacterium wolfei, they were transformed into protoplasts. The protoplasts, which possessed no cell wall, lysed in buffer without sucrose. Unlike whole cells, the...

journal_title：Journal of bacteriology

authors： Mountfort DO,Mörschel E,Beimborn DB,Schönheit P

更新日期：1986-11-01 00:00:00

abstract：:The importance of proton movements was assessed in the moderate halophile Vibrio costicola. When anaerobic cells in acidic buffer (pH 6.5) were given an O2 pulse, protons were extruded regardless of the presence of Na+. At pH 8.5, however, V. costicola produced an acidic response to an O2 pulse in the absence of Na+ a...

journal_title：Journal of bacteriology

authors： Hamaide F,Kushner DJ,Sprott GD

更新日期：1985-02-01 00:00:00

abstract：:Pseudomonas savastanoi pv. savastanoi strains harbor native plasmids belonging to the pPT23A plasmid family (PFPs) which are detected in all pathovars of the related species Pseudomonas syringae examined and contribute to the ecological and pathogenic fitness of their host. However, there is a general lack of informat...

journal_title：Journal of bacteriology

authors： Pérez-Martínez I,Zhao Y,Murillo J,Sundin GW,Ramos C

更新日期：2008-01-01 00:00:00

abstract：:Tests for utilization of d-octopine and nopaline in defined media containing a carbon and nitrogen source were made on 60 strains of Agrobacterium representing four species and on a representative of each of five species of Rhizobium. Among 46 virulent strains of Agrobacterium, only two strains were found which utiliz...

journal_title：Journal of bacteriology

authors： Lippincott JA,Beiderbeck R,Lippincott BB

更新日期：1973-10-01 00:00:00

abstract：:Alginate-producing (mucoid) strains of Pseudomonas aeruginosa possess a 54-kDa outer membrane (OM) protein (AlgE) which is missing in nonmucoid bacteria. The coding region of the algE gene from mucoid P. aeruginosa CF3/M1 was subcloned in the expression vector pT7-7 and expressed in Escherichia coli. The level of expr...

journal_title：Journal of bacteriology

authors： Rehm BH,Boheim G,Tommassen J,Winkler UK

更新日期：1994-09-01 00:00:00

abstract：:Chlamydophila pneumoniae is a gram-negative obligate intracellular bacterial pathogen that causes pneumonia and bronchitis and may contribute to atherosclerosis. The developmental cycle of C. pneumoniae includes a morphological transition from an infectious extracellular elementary body (EB) to a noninfectious intrace...

journal_title：Journal of bacteriology

authors： Johnson DL,Stone CB,Mahony JB

更新日期：2008-04-01 00:00:00

abstract：:The penicillin-binding proteins (PBPs) are a set of enzymes that participate in bacterial peptidoglycan assembly. The absolute numbers of each PBP were determined by direct measurement and have been reported for two Staphylococcus aureus strains, RN4220 (methicillin-sensitive S. aureus) and RN450M (methicillin-resista...

journal_title：Journal of bacteriology

authors： Pucci MJ,Dougherty TJ

更新日期：2002-01-01 00:00:00

abstract：:As a step towards studying representative members of the two-component family of signal transduction proteins, we have cloned genes encoding a histidine protein kinase and a response regulator from the hyperthermophilic bacterium Thermotoga maritima. The genes have been designated HpkA and drrA, respectively. The dedu...

journal_title：Journal of bacteriology

authors： Lee PJ,Stock AM

更新日期：1996-10-01 00:00:00

abstract：:A cloned 8.3-kilobase-pair DNA fragment carrying all the genes (iucABCD iutA) of the aerobactin iron transport system of plasmid pColV-K30 was subjected to in vitro mutagenesis to afford mutant genes iucA, iucC, and iucA iucC. Complementation analyses and identification of aerobactin precursors accumulated by Escheric...

journal_title：Journal of bacteriology

authors： de Lorenzo V,Neilands JB

更新日期：1986-07-01 00:00:00

abstract：:DNA helicase I, encoded on the Escherichia coli F plasmid, catalyzes a site- and strand-specific nicking reaction within the F plasmid origin of transfer (oriT) to initiate conjugative DNA strand transfer. The product of the nicking reaction contains a single phosphodiester bond interruption as determined by single-nu...

journal_title：Journal of bacteriology

authors： Matson SW,Nelson WC,Morton BS

更新日期：1993-05-01 00:00:00

abstract：:Escherichia coli LD0181 is sensitive to 15 micrograms of 2',3'-dideoxythymidine per ml. A derivative that was resistant to 40 micrograms of the same chemical per ml at 30 degrees C and that had lost the ability to grow on enriched medium at 42 degrees C was isolated after nitroso-guanidine mutagenesis. This mutant, TD...

journal_title：Journal of bacteriology

authors： Daws TD,Fuchs JA

更新日期：1984-02-01 00:00:00

abstract：:Because very little is known about cell division in noncylindrical bacteria and cyanobacteria, we investigated 10 putative cytokinetic proteins in the unicellular spherical cyanobacterium Synechocystis strain PCC 6803. Concerning the eight penicillin-binding proteins (PBPs), which define three classes, we found that S...

journal_title：Journal of bacteriology

authors： Marbouty M,Mazouni K,Saguez C,Cassier-Chauvat C,Chauvat F

更新日期：2009-08-01 00:00:00

abstract：:Two-component signal transduction systems are utilized by prokaryotic and eukaryotic cells to sense and respond to environmental stimuli, both to maintain homeostasis and to rapidly adapt to changing conditions. Studies have begun to emerge that utilize a large-scale mutagenesis approach to analyzing these systems in ...

journal_title：Journal of bacteriology

authors： Hussa EA,O'Shea TM,Darnell CL,Ruby EG,Visick KL

更新日期：2007-08-01 00:00:00

abstract：:In bacteria, the 4-amino-hydroxymethyl-2-methylpyrimidine (HMP) moiety of thiamine is synthesized from 5-aminoimidazole ribotide (AIR), a branch point metabolite of purine and thiamine biosynthesis. ThiC is a member of the radical S-adenosylmethionine (AdoMet) superfamily and catalyzes the complex chemical rearrangeme...

journal_title：Journal of bacteriology

authors： Palmer LD,Dougherty MJ,Downs DM

更新日期：2012-11-01 00:00:00

abstract：:Archaea, constituting the third primary domain of life, harbor a basal transcription apparatus of the eukaryotic type, whereas curiously, a large fraction of the potential transcription regulation factors appear to be of the bacterial type. To date, little information is available on these predicted regulators and on ...

journal_title：Journal of bacteriology

authors： Enoru-Eta J,Gigot D,Thia-Toong TL,Glansdorff N,Charlier D

更新日期：2000-07-01 00:00:00