Life cycle of Neurospora crassa viewed by scanning electron microscopy.

Abstract:

:Scanning electron microscopy was used to examine the major stages of the life cycle of two wild-type strains of Neurospora crassa Shear and Dodge (St. Lawrence 3.1a and 74A): mycelia, protoperithecium formation, perithecia, ascospores, ascospore germination and outgrowth, macro and microconidia, and germination and outgrowth of macroconidia. Structures seen at the limit of resolution of bright-field and phase-contrast microscopes, e.g., the ribbed surface of ascospores, are well resolved. New details of conidial development and surface structure are revealed. There appears to be only one distinguishable morphological difference between the two strains. The pattern of germination and outgrowth which seems relatively constant for strain 74A or strain 3.1a, appears to be different for each. Conidia from strain 3.1a almost always germinate from a site between interconidial attachment points; whereas the germ tubes of strain 74A usually emerge from or very near the interconidial attachment site. These germination patterns usually do not segregate 2:2 in asci dissected in order. This observation suggests that conidial germination pattern is not under the control of a single gene.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Seale T

doi

10.1128/JB.113.2.1015-1025.1973

subject

Has Abstract

pub_date

1973-02-01 00:00:00

pages

1015-25

issue

2

eissn

0021-9193

issn

1098-5530

journal_volume

113

pub_type

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