Mechanism of erythromycin-induced ermC mRNA stability in Bacillus subtilis.

Abstract:

:In Bacillus subtilis, the ermC gene encodes an mRNA that is unusually stable (40-min half-life) in the presence of erythromycin, an inducer of ermC gene expression. A requirement for this induced mRNA stability is a ribosome stalled in the ermC leader region. This property of ermC mRNA was used to study the decay of mRNA in B. subtilis. Using constructs in which the ribosome stall site was internal rather than at the 5' end of the message, we show that ribosome stalling provides stability to sequences downstream but not upstream of the ribosome stall site. Our results indicate that ermC mRNA is degraded by a ribonucleolytic activity that begins at the 5' end and degrades the message in a 5'-to-3' direction.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Bechhofer DH,Zen KH

doi

10.1128/jb.171.11.5803-5811.1989

subject

Has Abstract

pub_date

1989-11-01 00:00:00

pages

5803-11

issue

11

eissn

0021-9193

issn

1098-5530

journal_volume

171

pub_type

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