Regulation of phospholipid synthesis in Escherichia coli by guanosine tetraphosphate.

abstract：:XerC and XerD are site-specific recombinases of the lambda integrase family which resolve multimeric replicons to monomers by acting at specific sites such as cer, ckr, nmr, parB, and psi, which are found in plasmids, or at the dif site found in the Escherichia coli chromosome. By using Southern hybridizations to clon...

journal_title：Journal of bacteriology

authors： Sirois S,Szatmari G

更新日期：1995-07-01 00:00:00

abstract：:The mechanism for the extrusion of Na+ from Mycoplasma gallisepticum cells was examined. Na+ efflux from cells was studied by diluting 22Na+-loaded cells into an isoosmotic NaCl solution and measuring the residual 22Na+ in the cells. Uphill 22Na+ efflux was found to be glucose dependent and linear with time over a 60-...

journal_title：Journal of bacteriology

authors： Shirvan MH,Schuldiner S,Rottem S

更新日期：1989-08-01 00:00:00

abstract：:The formation of the five tryptophan biosynthetic enzymes of Neurospora crassa was shown to be derepressed in histidine-starved cells. This histidine-mediated derepression was not due to a lowered intracellular concentration of tryptophan in these cells. Furthermore, histidine-mediated derepression of tryptophan enzym...

journal_title：Journal of bacteriology

authors： Carsiotis M,Jones RF,Lacy AM,Cleary TJ,Fankhauser DB

更新日期：1970-10-01 00:00:00

abstract：:ShdA is a large outer membrane protein of the autotransporter family whose passenger domain binds the extracellular matrix proteins fibronectin and collagen I, possibly by mimicking the host ligand heparin. The ShdA passenger domain consists of approximately 1,500 amino acid residues that can be divided into two regio...

journal_title：Journal of bacteriology

authors： Kingsley RA,Abi Ghanem D,Puebla-Osorio N,Keestra AM,Berghman L,Bäumler AJ

更新日期：2004-08-01 00:00:00

abstract：:MalK is a peripheral cytoplasmic membrane protein that has multiple activities in Escherichia coli. It associates with integral cytoplasmic membrane proteins MalF and MalG to form the maltose transport complex (MalFGK), a member of the ATP-binding cassette (ABC) superfamily of proteins. In addition, MalK participates ...

journal_title：Journal of bacteriology

authors： Lippincott J,Traxler B

更新日期：1997-02-01 00:00:00

abstract：:Linker insertion mutagenesis was used to modify the paracrystalline surface layer (S-layer) protein (RsaA) of the gram-negative bacterium Caulobacter crescentus. Eleven unique BamHI linker insertions in the cloned rsaA gene were identified; at the protein level, these linker insertions introduced 4 to 6 amino acids at...

journal_title：Journal of bacteriology

authors： Bingle WH,Nomellini JF,Smit J

更新日期：1997-02-01 00:00:00

abstract：:In aquatic environments, Caulobacter spp. can be found at the boundary between liquid and air known as the neuston. I report an approach to study temporal features of Caulobacter crescentus colonization and pellicle biofilm development at the air-liquid interface and have defined the role of cell surface structures in...

journal_title：Journal of bacteriology

authors： Fiebig A

更新日期：2019-08-22 00:00:00

abstract：:The occurrence of succinic dehydrogenase [succinic:(acceptor) oxidoreductase, EC 1.3.99.1] in membrane fractions of Micrococcus lysodeikticus was investigated. The enzyme could be purified 10-fold, by deoxycholate treatment. Butanol extraction of membranes yielded an active fraction, nonsedimentable at 130,000 x g for...

journal_title：Journal of bacteriology

authors： Pollock JJ,Linder R,Salton MR

更新日期：1971-07-01 00:00:00

abstract：:Individually, the ribosomal proteins L1, L23, L36, and S6 are not essential for cell proliferation of Bacillus subtilis, but the absence of any one of these ribosomal proteins causes a defect in the formation of the 70S ribosomes and a reduced growth rate. In mutant strains individually lacking these ribosomal protein...

journal_title：Journal of bacteriology

authors： Akanuma G,Yamazaki K,Yagishi Y,Iizuka Y,Ishizuka M,Kawamura F,Kato-Yamada Y

更新日期：2018-08-24 00:00:00

abstract：:Pathogenic Yersinia species employ a type III secretion system (TTSS) to target antihost factors, Yop proteins, into eukaryotic cells. The secretion machinery is constituted of ca. 20 Ysc proteins, nine of which show significant homology to components of the flagellar TTSS. A key event in flagellar assembly is the swi...

journal_title：Journal of bacteriology

authors： Lavander M,Sundberg L,Edqvist PJ,Lloyd SA,Wolf-Watz H,Forsberg A

更新日期：2002-08-01 00:00:00

abstract：:Phosphorylation of a major 57-kilodalton protein substrate was observed in cell lysates of Spiroplasma melliferum BC3 incubated with [gamma-32P]ATP. Only serine phosphates have been isolated from the acid hydrolysate of the phosphorylated protein. The 57-kilodalton protein substrate was found, to a large extent, in th...

journal_title：Journal of bacteriology

authors： Platt MW,Reizer J,Rottem S

更新日期：1990-05-01 00:00:00

abstract：:The gene encoding a 45-kDa protein (45K) of Chlamydia trachomatis serovar F was cloned, sequenced, and overexpressed in Escherichia coli. Alignment of the deduced peptide sequence with E. coli elongation factor Tu (EF-Tu) demonstrated 69% identity. The 45K was recognized by a Chlamydia genus-specific monoclonal antibo...

journal_title：Journal of bacteriology

authors： Zhang YX,Shi Y,Zhou M,Petsko GA

更新日期：1994-02-01 00:00:00

abstract：:Mutants that require exogenous D-glucosamine for growth were isolated from Saccharomyces cerevisiae X2180-1A after ethyl methane sulfonate mutagenesis. Class A auxotrophs fail to grow on yeast extract-peptone-dextrose and minimal media, whereas class B auxotrophs grow on minimal medium and readily revert to grow on ye...

journal_title：Journal of bacteriology

authors： Whelan WL,Ballou CE

更新日期：1975-12-01 00:00:00

abstract：:Bishop, D. G. (University of Sydney, Sydney, Australia), and J. L. Still. Fatty acid metabolism in Serratia marcescens. J. Bacteriol. 82:370-375. 1961.-A study has been made of the oxidation of saturated fatty acids containing between two and 18 carbon atoms by whole cells of the bacterium, Serratia marcescens. This o...

journal_title：Journal of bacteriology

authors： Bishop DG,Still JL

更新日期：1961-09-01 00:00:00

abstract：:The GcvA protein is a LysR family regulatory protein necessary for both activation and repression of the Escherichia coli glycine cleavage enzyme operon (gcv) and negative regulation of gcvA. Gel shift assays indicated that overexpressed GcvA in crude extracts is capable of binding specifically to DNA containing the g...

journal_title：Journal of bacteriology

authors： Wilson RL,Urbanowski ML,Stauffer GV

更新日期：1995-09-01 00:00:00

abstract：:The morphological and physiological characteristics of 20 isolates of psychrophilic Bacillus were compared with 29 strains representing nine species of mesophilic Bacillus and 2 strains of Sporosarcina ureae to determine the taxonomic position of the psychrophiles. The psychrophiles formed four distinct groups which w...

journal_title：Journal of bacteriology

authors： Larkin JM,Stokes JL

更新日期：1967-10-01 00:00:00

abstract：:Western immunoblots and assays of Bacillus subtilis extracts showed that intracellular serine protease 1 is produced in a form larger than previously reported, appears not to have undergone N-terminal processing, and is active in the presence or absence of calcium. No evidence for an inactive precursor form of the pro...

journal_title：Journal of bacteriology

authors： Sheehan SM,Switzer RL

更新日期：1990-01-01 00:00:00

abstract：:Arginine deiminase activity was induced during the vegetative growth of Clostridium sporogenes. The enzyme was sensitive to catabolite repression. The other enzymes of the arginine dihydrolase pathway, namely, ornithine carbamoyl-transferase and carbamate kinase, did not show such variation. ...

journal_title：Journal of bacteriology

authors： Venugopal V,Nadkarni GB

更新日期：1977-08-01 00:00:00

abstract：:Plasmid ColIb-P9 of the I alpha incompatibility group is known to encode a DNA primase that acts in the conjugal transfer of the plasmid and can substitute for mutant dnaG gene product in vegetative replication of the Escherichia coli chromosome. The relevant genetic determinant (sog) has previously been cloned into a...

journal_title：Journal of bacteriology

authors： Dalrymple BP,Boulnois GJ,Wilkins BM,Orr E,Williams PH

更新日期：1982-07-01 00:00:00

abstract：:Escherichia coli fhuF mutants, a sufS::MudI mutant, and a sufD::MudI mutant were found to have the same phenotype: the inability to use ferrioxamine B as an iron source in a plate assay. In addition, the sufS and sufD genes were shown to be regulated by the iron-dependent Fur repressor. Sequence analysis revealed that...

journal_title：Journal of bacteriology

authors： Patzer SI,Hantke K

更新日期：1999-05-01 00:00:00

abstract：:Of the nine genes comprising the L-rhamnose operon of Rhizobium leguminosarum, rhaU has not been assigned a function. The construction of a Delta rhaU strain revealed a growth phenotype that was slower than that of the wild-type strain, although the ultimate cell yields were equivalent. The transport of L-rhamnose int...

journal_title：Journal of bacteriology

authors： Richardson JS,Carpena X,Switala J,Perez-Luque R,Donald LJ,Loewen PC,Oresnik IJ

更新日期：2008-04-01 00:00:00

abstract：:The lining of the maltodextrin-specific maltoporin (LamB) channel exhibits a string of aromatic residues, the greasy slide, part of which has been shown previously by crystallography to be involved in substrate binding. To probe the functional role of the greasy slide, alanine scanning mutagenesis has been performed o...

journal_title：Journal of bacteriology

authors： Van Gelder P,Dumas F,Bartoldus I,Saint N,Prilipov A,Winterhalter M,Wang Y,Philippsen A,Rosenbusch JP,Schirmer T

更新日期：2002-06-01 00:00:00

abstract：:A mutator mutation of Salmonella typhimurium, observed in a strain resistant to P22 and rifampin, affects mutation rates of bacterial, R factor, and prophage P22 genes. ...

journal_title：Journal of bacteriology

authors： Guterman SK,Wright A

更新日期：1974-08-01 00:00:00

abstract：:We constructed mercury resistance operon-luciferase (mer-lux) transcriptional fusion plasmids to evaluate in vivo gene expression rates of the mer structural gene promoter (PTPCAD) of transposon Tn21. In vivo gene expression kinetics corresponded well with those previously determined in vitro, yielding an apparent K0....

journal_title：Journal of bacteriology

authors： Condee CW,Summers AO

更新日期：1992-12-01 00:00:00

abstract：:The highly defective rho-15 mutant of Escherichia coli produces plasmid DNA that is 22% less negatively supercoiled than DNA from an isogenic wild-type strain (J. S. Fassler, G. F. Arnold, and I. Tessman, Mol. Gen. Genet. 204:424-429, 1986). We extended our measurements of plasmid superhelicity to additional rho mutan...

journal_title：Journal of bacteriology

authors： Arnold GF,Tessman I

更新日期：1988-09-01 00:00:00

abstract：:Freter, Rolf (Jefferson Medical College, Philadelphia, Pa.), and Atsushi Ozawa. Explanation for limitation of populations of Escherichia coli in broth cultures. J. Bacteriol. 86:904-910. 1963.-Veal Infusion Broth surrounding a cellophane bag containing a 24-hr culture of Escherichia coli was inhibitory to an inoculum ...

journal_title：Journal of bacteriology

authors： FRETER R,OZAWA A

更新日期：1963-11-01 00:00:00

abstract：:We describe here the cloning of single EcoRI fragments from the tra region of F DNA using ColE1::Tn3 as vector. These plasmids, as well as the series of Skurray et al. (Proc. Natl. Acad. Sci. U.S.A. 73:64-68, 1976), have been used to refine the map positions of tra cistrons on the F factor as well as to define a new D...

journal_title：Journal of bacteriology

authors： Achtman M,Skurray RA,Thompson R,Helmuth R,Hall S,Beutin L,Clark AJ

更新日期：1978-03-01 00:00:00

abstract：:A compound, isolated from the culture fluid of a nadC auxotroph of Escherichia coli grown in a minimal medium, supports the growth of both a nadA and a nadB mutant. This metabolite exhibits an ultraviolet light absorption spectrum and a mass spectrum, different from quinolinic acid. This compound may be the precursor ...

journal_title：Journal of bacteriology

authors： Chen JL,Tritz GJ

更新日期：1975-01-01 00:00:00

abstract：:Wright, D. N. (Iowa State University, Ames), and W. R. Lockhart. Environmental control of cell composition in Escherichia coli. J. Bacteriol. 89:1026-1031. 1965.-Escherichia coli was grown in continuous culture at various rates in a defined medium with either the carbon or the nitrogen source as rate-limiting substrat...

journal_title：Journal of bacteriology

authors： WRIGHT DN,LOCKHART WR

更新日期：1965-04-01 00:00:00

abstract：:Two Rhizobium phaseoli mutants, isolated previously by Tn5 mutagenesis, elicited infection threads which ceased development prematurely, usually within root hairs. These infection threads were wide, globular, and otherwise altered in morphology, compared with normal infection threads. Anatomy and division of the root ...

journal_title：Journal of bacteriology

authors： Noel KD,Vandenbosch KA,Kulpaca B

更新日期：1986-12-01 00:00:00