Abstract:
:Wright, D. N. (Iowa State University, Ames), and W. R. Lockhart. Environmental control of cell composition in Escherichia coli. J. Bacteriol. 89:1026-1031. 1965.-Escherichia coli was grown in continuous culture at various rates in a defined medium with either the carbon or the nitrogen source as rate-limiting substrate. Average cell size and the cellular content of ribonucleic acid, protein, and free amino acids varied both with growth rate and with the identity of the limiting nutrient, being greatest at high growth rates, and greater for nitrogen-limited than for carbon-limited populations. Immunoelectrophoresis of cell extracts revealed antigenic components associated uniquely with growth rate, and others associated with the identity of the limiting nutrient on which cells had been grown. Cell composition thus appears to reflect the physiological adjustment of a microbial population to its total environment.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
WRIGHT DN,LOCKHART WRdoi
10.1128/JB.89.4.1026-1031.1965keywords:
subject
Has Abstractpub_date
1965-04-01 00:00:00pages
1026-31eissn
0021-9193issn
1098-5530journal_volume
89pub_type
杂志文章abstract::A primer extension inhibition (toeprint) assay was developed using ribosomes and ribosomal subunits from Streptomyces lividans. This assay allowed the study of ribosome binding to streptomycete leaderless and leadered mRNA. Purified 30S subunits were unable to form a ternary complex on aph leaderless mRNA, whereas 70S...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.20.6864-6875.2004
更新日期:2004-10-01 00:00:00
abstract:UNLABELLED:Deacetylation of 7-aminocephalosporanic acid (7-ACA) at position C-3 provides valuable starting material for producing semisynthetic β-lactam antibiotics. However, few enzymes have been characterized in this process before now. Comparative analysis of the genome of the thermophilic bacterium Alicyclobacillus...
journal_title:Journal of bacteriology
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doi:10.1128/JB.00471-15
更新日期:2015-11-02 00:00:00
abstract::The genomes of most strains of Salmonella and Escherichia coli are highly conserved. In contrast, all 136 wild-type strains of Salmonella enterica serovar Typhi analyzed by partial digestion with I-CeuI (an endonuclease which cuts within the rrn operons) and pulsed-field gel electrophoresis and by PCR have rearrangeme...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.8.2638-2650.2005
更新日期:2005-04-01 00:00:00
abstract::The effect of plasmid CAM-OCT on responses to UV irradiation was compared in Pseudomonas aeruginosa, in Pseudomonas putida, and in Pseudomonas putida mutants carrying mutations in UV response genes. CAM-OCT substantially increased both survival and mutagenesis in the two species. P. aeruginosa strains without CAM-OCT ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.2.975-982.1989
更新日期:1989-02-01 00:00:00
abstract::The glycan chains in peptidoglycan or murein are cross-linked by transpeptidation of the peptide side chains. To assess the fraction of side chains involved in cross-bridges, distinction has been made between cross-linkage and cross-linking. The first expression refers to the situation in unlabeled (or fully labeled) ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.6.2028-2031.1992
更新日期:1992-03-01 00:00:00
abstract::Expression of a circadian rhythm of conidiation in Neurospora crassa is enhanced in cultures grown on medium that is neutral or deficient in biotin. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.123.1.387-389.1975
更新日期:1975-07-01 00:00:00
abstract::Desulfovibrio vulgaris Hildenborough, a sulfate-reducing bacterium classified as an obligate anaerobe, swam to a preferred oxygen concentration of 0.02 to 0.04% (0.24 to 0.48 microM), a level which also supported growth. Oxygen concentrations of 0.08% and higher arrested growth. We propose that in zones of transition ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.17.5598-5601.1997
更新日期:1997-09-01 00:00:00
abstract::The N-terminal amino acid sequence of the polyhydroxyalkanoic acid (PHA) granule-associated M(r)-15,500 protein of Rhodococcus ruber (the GA14 protein) was analyzed. The sequence revealed that the corresponding structural gene is represented by open reading frame 3, encoding a protein with a calculated M(r) of 14,175 ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.14.4328-4337.1994
更新日期:1994-07-01 00:00:00
abstract::The control of expression of the Bacillus subtilis spoIIA locus was analyzed by titrating gene expression against gene copy number. A plasmid integrated into the B. subtilis chromosome and carrying the spoIIA control region fused to Escherichia coli lacZ was forced to form tandem repeats by the selection of clones tha...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.3.1260-1266.1987
更新日期:1987-03-01 00:00:00
abstract::Holwerda, Jack (Michigan Department of Health, Grand Rapids) and Grace Eldering. Culture and fluorescent-antibody methods in diagnosis of whooping cough. J. Bacteriol. 86:449-451. 1963.-Nasopharyngeal swabs from 517 suspected whooping-cough patients were examined by culture and by fluorescent-antibody (FA) staining pr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.86.3.449-451.1963
更新日期:1963-09-01 00:00:00
abstract::A low level of recombination between homologous regions of plasmids and the host chromosome occurred during cell growth. The plasmids contained antibiotic resistance markers on the homologous regions which were only expressed when they were integrated into the chromosome. Such recombination took place in Rec+ rec-2 mu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.168.1.458-459.1986
更新日期:1986-10-01 00:00:00
abstract::ATP sulfurylase is a key enzyme in the energy-generating sulfur oxidation pathways of many chemoautotrophic bacteria. The utilization of reduced sulfur compounds to fuel CO2 fixation by the still-uncultured bacterial endosymbionts provides the basis of nutrition in invertebrates, such as the tubeworm Riftia pachyptila...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.12.3723-3729.1994
更新日期:1994-06-01 00:00:00
abstract::Grunberg, E. (Hoffman-La Roche, Inc., Nutley, N.J.), and R. Cleeland. Fluorescence and viability of Proteus mirabilis stained directly with fluorescein isothiocyanate. J. Bacteriol. 92:23-27. 1966.-Washed cell suspensions of Proteus mirabilis, under the proper conditions, stained well with fluorescein isothiocyanate w...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.92.1.23-27.1966
更新日期:1966-07-01 00:00:00
abstract::An obligate thermophilic strain of Bacillus stearothermophilus, strain PH24, isolated from industrial sediment by elective culture, grew readily at 55 C on phenol or on one of the isomers of cresol as the major carbon source. Intact cells grown in the presence of phenol, o-cresol, m-cresol, or p-cresol were induced to...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.124.3.1077-1083.1975
更新日期:1975-12-01 00:00:00
abstract::The mobilizable Bacteroides element NBU2 (11 kbp) was found originally in two Bacteroides clinical isolates, Bacteroides fragilis ERL and B. thetaiotaomicron DOT. At first, NBU2 appeared to be very similar to another mobilizable Bacteroides element, NBU1, in a 2.5-kbp internal region, but further examination of the fu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.12.3559-3571.2000
更新日期:2000-06-01 00:00:00
abstract::The nitrogenase proteins from eight organisms have been highly purified, and a survey of their cross-reactions shows that the nitrogenase proteins from a wide variety of organisms can interact with one another. An active cross-reaction is the complementary functioning of the MoFe protein and the Fe protein from differ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.134.3.936-943.1978
更新日期:1978-06-01 00:00:00
abstract::Synchrony in phototrophic cultures of Rhodospirillum rubrum was induced by stationary-phase cycling or by alterations in light intensity. Intracytoplasmic chromatophore membranes were prepared by differential centrifugation. Analysis of the composition of chromatophores obtained from cells at different times indicated...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.166.3.818-823.1986
更新日期:1986-06-01 00:00:00
abstract::Ganesan, Ann K. (Syntex Institute of Molecular Biology, Palo Alto, Calif.), and Boris Rotman. Transfer and incorporation of genes controlling beta-d-galactosidase synthesis from Hfr and F' donors of Escherichia coli. J. Bacteriol. 92:1378-1382. 1966.-Comparisons were made between Hfr(1) and F(13) donors with respect t...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.92.5.1378-1382.1966
更新日期:1966-11-01 00:00:00
abstract::In Zymomonas mobilis, three- to fourfold more glyceraldehyde-3-phosphate dehydrogenase protein than phosphoglycerate kinase is needed for glycolysis because of differences in catalytic efficiency. Consistent with this requirement, higher levels of glyceraldehyde-3-phosphate dehydrogenase were observed with two-dimensi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.1.245-254.1991
更新日期:1991-01-01 00:00:00
abstract::The DosS (DevS) and DosT histidine kinases form a two-component system together with the DosR (DevR) response regulator in Mycobacterium tuberculosis. DosS and DosT, which have high sequence similarity to each other over the length of their amino acid sequences, contain two GAF domains (GAF-A and GAF-B) in their N-ter...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00550-10
更新日期:2010-10-01 00:00:00
abstract::Nitrogenase activities were determined from maximum acetylene reduction rates for mutant strains of Azotobacter vinelandii which are unable to fix N2 in the presence of molybdenum (Nif-) but undergo phenotypic reversal to Nif+ under conditions of Mo deficiency. The system responsible for N2 fixation under these condit...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.150.3.1244-1251.1982
更新日期:1982-06-01 00:00:00
abstract::The pyrimidine de novo biosynthesis pathway has been characterized for a number of organisms. The general pathway consists of six enzymatic steps. In the characterization of the pyrimidine pathway of Lactococcus lactis, two different pyrD genes encoding dihydroorotate dehydrogenase were isolated. The nucleotide sequen...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.13.3975-3982.1994
更新日期:1994-07-01 00:00:00
abstract::A transformable strain of Bacillus subtilis 6160, a derivative of B. subtilis 168, produces three kinds of casein hydrolytic enzymes (alkaline protease, neutral protease, and esterase) in a culture medium. B. natto IAM 1212 produces 15 to 20 times as much total proteolytic activity as does B. subtilis. Extracellular p...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.119.1.82-91.1974
更新日期:1974-07-01 00:00:00
abstract::Valentine, R. C. (University of Illinois, Urbana), H. Drucker, and R. S. Wolfe. Glyoxylate fermentation by Streptococcus allantoicus. J. Bacteriol. 87:241-246. 1964.-Extracts of Streptococcus allantoicus were found to degrade glyoxylate, yielding tartronic semialdehyde and CO(2). Tartronic semialdehyde was prepared ch...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.87.2.241-246.1964
更新日期:1964-02-01 00:00:00
abstract::The intermolecular interactions of the mycobacteriophage Ms6 secretion chaperone with endolysin were characterized. The 384-amino-acid lysin (lysin(384))-binding domain was found to encompass the N-terminal region of Gp1, which is also essential for a lysis phenotype in Escherichia coli. In addition, a GXXXG-like moti...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00380-11
更新日期:2011-09-01 00:00:00
abstract::Biofilm formation is a key factor in Vibrio cholerae environmental survival and host colonization. Production of biofilm enables V. cholerae to survive and persist in aquatic environments and aids in the passage through the gastric acid barrier to allow access to the small intestine. The genes involved in biofilm form...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00014-12
更新日期:2012-06-01 00:00:00
abstract::Eight different derivatives of R plasmid RP1 with thermosensitive mutations affecting maintenance in Escherichia coli and Pseudomonas aeruginosa were introduce into Rhizobium meliloti. None of the plasmids showed a thermosensitive character in R. meliloti. On the other hand, a certain deletion mutation in RP1 was foun...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.150.1.395-397.1982
更新日期:1982-04-01 00:00:00
abstract::A hypothesis that preferential rejection of donor markers by the hex system of pneumococcus is due to lethal double-strand breaks has been examined in terms of its implications for the extent of the excision required. Experiments reported here were directed at asking whether hex-dependent marker efficiency depends on ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.125.1.125-135.1976
更新日期:1976-01-01 00:00:00
abstract::MurG is a peripheral membrane protein that is one of the key enzymes in peptidoglycan biosynthesis. The crystal structure of Escherichia coli MurG (S. Ha, D. Walker, Y. Shi, and S. Walker, Protein Sci. 9:1045-1052, 2000) contains a hydrophobic patch surrounded by basic residues that may represent a membrane associatio...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.13.3773-3779.2003
更新日期:2003-07-01 00:00:00
abstract::The biosynthesis of the thaxtomin cyclic dipeptide phytotoxins proceeds nonribosomally via the thiotemplate mechanism. Acyladenylation, thioesterification, N-methylation, and cyclization of two amino acid substrates are catalyzed by the txtAB-encoded thaxtomin synthetase. Nucleotide sequence analysis of the region 3' ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.7.2019-2029.2002
更新日期:2002-04-01 00:00:00