Kinetic analysis of tRNA-directed transcription antitermination of the Bacillus subtilis glyQS gene in vitro.

abstract：:A pentachlorophenol (PCP) hydroxylase which catalyzed the conversion of PCP to 2,3,5,6-tetrachlorohydroquinone and released iodide from triiodophenol in the presence of NADPH and oxygen was identified. The enzyme was purified by protamine sulfate precipitation, ammonium sulfate precipitation, hydrophobic chromatograph...

journal_title：Journal of bacteriology

authors： Xun L,Orser CS

更新日期：1991-07-01 00:00:00

abstract：:Growth of Escherichia coli in the presence of certain D-amino acids, such as D-methionine, results in the incorporation of the D-amino acid into macromolecular peptidoglycan and can be lethal at high concentrations. Previous studies suggested that incorporation was independent of the normal biosynthetic pathway. An en...

journal_title：Journal of bacteriology

authors： Caparrós M,Pisabarro AG,de Pedro MA

更新日期：1992-09-01 00:00:00

abstract：:Utilizing a homologous gene replacement in order to retain the native promoter and 5' and 3' untranslated messenger regions (and thereby ensure physiological validity), we have shown that deletion of the N-terminal 389 amino acids of the transcriptional activator AREA does not result in nitrogen metabolite derepressio...

journal_title：Journal of bacteriology

authors： Caddick MX,Arst HN Jr

更新日期：1998-11-01 00:00:00

abstract：:Klebsiella pneumoniae can use nitrate and nitrite as sole nitrogen sources through the nitrate assimilatory pathway. The structural genes for assimilatory nitrate and nitrite reductases together with genes necessary for nitrate transport form an operon, nasFEDCBA. Expression of the nasF operon is regulated both by gen...

journal_title：Journal of bacteriology

authors： Goldman BS,Lin JT,Stewart V

更新日期：1994-08-01 00:00:00

abstract：:Proteus rettgeri and Escherichia coli W were shown to express structurally different penicillin G acylases. The enzymes had similar substrate specificity but differed in molecular weight, isoelectric point, and electrophoretic mobility in polyacrylamide gels and did not antigenically cross-react. When the organisms we...

journal_title：Journal of bacteriology

authors： Daumy GO,Danley D,McColl AS,Apostolakos D,Vinick FJ

更新日期：1985-09-01 00:00:00

abstract：:We have determined the amounts of a number of small molecules and enzymes in the mother cell compartment and the developing forespore during sporulation of Bacillus megaterium. Significant amounts of adenosine 5'-triphosphate and reduced nicotinamide adenine dinucleotide were present in the forespore compartment befor...

journal_title：Journal of bacteriology

authors： Singh RP,Setlow B,Setlow P

更新日期：1977-06-01 00:00:00

abstract：:Here we describe that the Helicobacter pylori sensor kinase produced by HP1364 and the response regulator produced by HP1365 and designated CrdS and CrdR, respectively, are both required for transcriptional induction of the H. pylori copper resistance determinant CrdA by copper ions. CrdRS-deficient mutants lacked cop...

journal_title：Journal of bacteriology

authors： Waidner B,Melchers K,Stähler FN,Kist M,Bereswill S

更新日期：2005-07-01 00:00:00

abstract：:Studies of mitochondrial biogenesis in yeast have been hampered by a lack of suitable membrane markers in anaerobically grown cells subsequently grown in air. Cytochrome c peroxidase activity and subcellular location was studied to determine whether it would be a useful marker for an analysis of mitochondrial formatio...

journal_title：Journal of bacteriology

authors： Avers CJ

更新日期：1967-10-01 00:00:00

abstract：:LéJohn, Herbert B. (Purdue University, Lafayette, Ind.), and James S. Lovett. Ribonucleic acid and protein synthesis in Rhizophlyctis rosea zoospores. J. Bacteriol. 91:709-717. 1966.-The uniflagellate zoospores of Rhizophlyctis rosea display active motility and a high endogenous respiratory metabolism, but neither gro...

journal_title：Journal of bacteriology

authors： Léjohn HB,Lovett JS

更新日期：1966-02-01 00:00:00

abstract：:Expression of the Bacillus licheniformis alpha-amylase gene, amyL, was temporally activated and subject to catabolite repression both in its natural host and when cloned on a 3.55-kilobase fragment in Bacillus subtilis. A subclone from which the promoter region of amyL and sequences upstream from the promoter were del...

journal_title：Journal of bacteriology

authors： Laoide BM,Chambliss GH,McConnell DJ

更新日期：1989-05-01 00:00:00

abstract：:The products of in-frame overlapping genes II and X carried by the filamentous phage f1 genome are proteins with required but opposing functions in phage DNA replication. Their normal relative levels are important for continuous production of phage DNA without killing infected Escherichia coli hosts. Here we identify ...

journal_title：Journal of bacteriology

authors： Kokoska RJ,Steege DA

更新日期：1998-06-01 00:00:00

abstract：:Lysozyme Chalaropsis, a beta-1,4-N,6-O-diacetylmuramidase, attacks the cell wall murein of Staphylococcus aureus H in a random manner. In hypertonic sucrose solutions, true protoplasts are produced and, in the process, mesosomal tubules are extruded into the medium. ...

journal_title：Journal of bacteriology

authors： Robinson JP,Robinson RD,Hash JH

更新日期：1974-02-01 00:00:00

abstract：:We have cloned the Micromonospora viridifaciens neuraminidase (EC 3.2.1.18) gene (nedA) in Streptomyces lividans. This was accomplished by using the vector pIJ702 and BglII-BclI libraries of M. viridifaciens chromosomal inserts created in S. lividans. The libraries were screened for the expression of neuraminidase by ...

journal_title：Journal of bacteriology

authors： Sakurada K,Ohta T,Hasegawa M

更新日期：1992-11-01 00:00:00

abstract：:As part of our effort to uncover the molecular basis for the phenotypic variation among clinical Mycobacterium tuberculosis isolates, we have previously reported that isolates belonging to the W/Beijing lineage constitutively overexpress the DosR-regulated transcriptional program. While generating dosR knockouts in tw...

journal_title：Journal of bacteriology

authors： Domenech P,Kolly GS,Leon-Solis L,Fallow A,Reed MB

更新日期：2010-09-01 00:00:00

abstract：:The formation of the five tryptophan biosynthetic enzymes of Neurospora crassa was shown to be derepressed in histidine-starved cells. This histidine-mediated derepression was not due to a lowered intracellular concentration of tryptophan in these cells. Furthermore, histidine-mediated derepression of tryptophan enzym...

journal_title：Journal of bacteriology

authors： Carsiotis M,Jones RF,Lacy AM,Cleary TJ,Fankhauser DB

更新日期：1970-10-01 00:00:00

abstract：:The bacterial flagellum is a biological nanomachine that rotates to allow bacteria to swim. For flagellar rotation, torque is generated by interactions between a rotor and a stator. The stator, which is composed of MotA and MotB subunit proteins in the membrane, is thought to bind to the peptidoglycan (PG) layer, whic...

journal_title：Journal of bacteriology

authors： Zhu S,Nishikino T,Takekawa N,Terashima H,Kojima S,Imada K,Homma M,Liu J

更新日期：2020-01-29 00:00:00

abstract：:Plasmid pUB110 was previously used as a vector to clone fragments of deoxyribonucleic acid that complement the trpC2 mutation in Bacillus subtilis from endonuclease EcoRI digested B. licheniformis, B. pumilus, and B. subtilis cellular deoxyribonucleic acid. Each of several such trp plasmids was subsequently shown to c...

journal_title：Journal of bacteriology

authors： Keggins KM,Lovett PS,Marrero R,Hoch SO

更新日期：1979-09-01 00:00:00

abstract：:The fucose-/mannose-specific lectin LecB from Pseudomonas aeruginosa is transported to the outer membrane; however, the mechanism used is not known so far. Here, we report that LecB is present in the periplasm of P. aeruginosa in two variants of different sizes. Both were functional and could be purified by their affi...

journal_title：Journal of bacteriology

authors： Bartels KM,Funken H,Knapp A,Brocker M,Bott M,Wilhelm S,Jaeger KE,Rosenau F

更新日期：2011-03-01 00:00:00

abstract：:Borrelia burgdorferi, the Lyme disease agent, and other members of the spirochetal genus Borrelia have double-stranded linear plasmids in addition to supercoiled circular plasmids. The copy number relative to the chromosome was determined for 49- and 16-kb linear plasmids and a 27-kb circular plasmid of the type strai...

journal_title：Journal of bacteriology

authors： Hinnebusch J,Barbour AG

更新日期：1992-08-01 00:00:00

abstract：:Davis, Nour A. (University of Lagos Medical School, Lagos, Nigeria), and G. H. G. Davis. Ecology of nasal staphylococci. J. Bacteriol. 89:1163-1168. 1965.-The rate of nasal carriage of Staphylococcus aureus in Nigerian adults (46%) approximates that found in other countries. The rate in infants under 12 months was ca....

journal_title：Journal of bacteriology

authors： DAVIS NA,DAVIS GH

更新日期：1965-05-01 00:00:00

abstract：:We have detected a cholesterol-dependent cytolysin, which we have named mitilysin, in a small number of Streptococcus mitis isolates. We have sequenced the mitilysin gene from seven isolates of S. mitis. Comparisons with the pneumococcal pneumolysin gene show 15 amino acid substitutions. S. mitis appear to release mit...

journal_title：Journal of bacteriology

authors： Jefferies J,Nieminen L,Kirkham LA,Johnston C,Smith A,Mitchell TJ

更新日期：2007-01-01 00:00:00

abstract：:The ESX-1 (ESAT-6 system 1) secretion system plays a conserved role in the virulence of diverse mycobacterial pathogens, including the human pathogen Mycobacterium tuberculosis and M. marinum, an environmental mycobacterial species. The ESX-1 system promotes the secretion of protein virulence factors to the extracytop...

journal_title：Journal of bacteriology

authors： Bosserman RE,Nicholson KR,Champion MM,Champion PA

更新日期：2019-06-21 00:00:00

abstract：:We have discovered an unusual and complex regulatory network used by the phytopathogen Pseudomonas solanacearum to control transcription of eps, which encodes for production of its primary virulence factor, the exopolysaccharide EPS I. The major modules of this network were shown to be three separate signal transducti...

journal_title：Journal of bacteriology

authors： Huang J,Carney BF,Denny TP,Weissinger AK,Schell MA

更新日期：1995-03-01 00:00:00

abstract：:Shiga toxin (STX), a bacterial toxin produced by Shigella dysenteriae type 1, is a hexamer composed of five receptor-binding B subunits which encircle an alpha-helix at the carboxyl terminus of the enzymatic A polypeptide. Hybrid toxins constructed by fusing the A polypeptide sequences of STX and Shiga-like toxin type...

journal_title：Journal of bacteriology

authors： Jemal C,Haddad JE,Begum D,Jackson MP

更新日期：1995-06-01 00:00:00

abstract：:In contrast to Escherichia coli and Salmonella typhimurium, Bacillus subtilis could convert ethionine to S-adenosylethionine (SAE), as can Saccharomyces cerevisiae. This conversion was essential for growth inhibition by ethionine because metE mutants which were deficient in S-adenosylmethionine synthetase activity, we...

journal_title：Journal of bacteriology

authors： Allen ER,Orrego C,Wabiko H,Freese E

更新日期：1986-04-01 00:00:00

abstract：:Caulobacter is a distinctive genus of prosthecate bacteria. Because caulobacters adhere to surfaces and are found in diverse locales, their role in oligotrophic environments and bacterial biofilm communities is of interest. The phylogenetic relationships of a group of marine and freshwater caulobacters were examined i...

journal_title：Journal of bacteriology

authors： Stahl DA,Key R,Flesher B,Smit J

更新日期：1992-04-01 00:00:00

abstract：:Hyaluronate stimulated the formation of exolipase by Serratia marcescens. This ability was abolished when all carboxyl groups of hyaluronate were methyl esterified. Additional studies suggested that the biological inactivity of esterified hyaluronate should be ascribed to the reduced conformational order of the molecu...

journal_title：Journal of bacteriology

authors： Jäger KE,Winkler UK

更新日期：1979-09-01 00:00:00

abstract：:Self-resistance has been investigated in Streptomyces caelestis (producer of the lincosamide antibiotic celesticetin), from which a lincosamide resistance determinant (clr) has been isolated on a 1-kilobase DNA fragment and cloned in Streptomyces lividans. The clr product is a specific methylase which produces a singl...

journal_title：Journal of bacteriology

authors： Calcutt MJ,Cundliffe E

更新日期：1990-08-01 00:00:00

abstract：:DNA helicase I, encoded on the Escherichia coli F plasmid, catalyzes a site- and strand-specific nicking reaction within the F plasmid origin of transfer (oriT) to initiate conjugative DNA strand transfer. The product of the nicking reaction contains a single phosphodiester bond interruption as determined by single-nu...

journal_title：Journal of bacteriology

authors： Matson SW,Nelson WC,Morton BS

更新日期：1993-05-01 00:00:00

abstract：:A mutant requiring isoleucine and valine for growth, because of the absence of acetolactate synthase activity, has been isolated. At least one of three different genes (ilvG, ilvB, ilvI) is required for the expression of acetolactate synthase activity, thus suggesting the presence of three different acetolactate synth...

journal_title：Journal of bacteriology

authors： Guardiola J,De Felice M,Iaccarino M

更新日期：1974-10-01 00:00:00