Abstract:
:The products of in-frame overlapping genes II and X carried by the filamentous phage f1 genome are proteins with required but opposing functions in phage DNA replication. Their normal relative levels are important for continuous production of phage DNA without killing infected Escherichia coli hosts. Here we identify several factors responsible for determining the relative levels of pII and pX and that, if perturbed, alter the normal distribution of the phage DNA species in infected hosts. Translation of the two proteins is essentially relegated to separate mRNAs. The mRNAs encoding genes II and X are also differentially sensitive to cleavage dependent on rne, the gene encoding the only E. coli endo-RNase known to have a global role in mRNA stability. Whereas pII levels are limited at the level of mRNA stability, normal pX levels require transcription in sufficient amounts from the promoter for the smaller mRNA encoding only pX.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Kokoska RJ,Steege DAdoi
10.1128/JB.180.12.3245-3249.1998subject
Has Abstractpub_date
1998-06-01 00:00:00pages
3245-9issue
12eissn
0021-9193issn
1098-5530journal_volume
180pub_type
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pub_type: 杂志文章
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.109.3.993-1000.1972
更新日期:1972-03-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:2010-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:1968-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:1997-09-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.116.2.663-672.1973
更新日期:1973-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.1.166-175.1993
更新日期:1993-01-01 00:00:00
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更新日期:1987-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:1999-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:1989-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1995-05-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/jb.173.14.4493-4502.1991
更新日期:1991-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1977-11-01 00:00:00