The initiation codon affects ribosome binding and translational efficiency in Escherichia coli of cI mRNA with or without the 5' untranslated leader.

Abstract:

:Translational efficiency of an AUG, CUG, GUG, or UUG initiation codon was measured for the naturally leaderless cI mRNA from bacteriophage lambda. In a cI-lacZ translational fusion, only AUG supported a high level of expression; GUG supported a low level of expression, while UUG and CUG expression was barely above background levels. Addition of an untranslated lac leader and Shine-Dalgarno sequence to cI increased expression but still showed a dependence on an AUG for maximum expression. cI-lacZ mRNA with an AUG initiation codon showed a greater in vitro ribosome binding strength and a higher level of full-length in vivo mRNA, suggesting that the initiation codon is an important determinant of ribosome binding strength and translational efficiency for mRNA with or without the 5' untranslated leader.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

O'Donnell SM,Janssen GR

doi

10.1128/JB.183.4.1277-1283.2001

keywords:

subject

Has Abstract

pub_date

2001-02-01 00:00:00

pages

1277-83

issue

4

eissn

0021-9193

issn

1098-5530

journal_volume

183

pub_type

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