Abstract:
:We demonstrate that the expression of hem genes in Rhodobacter capsulatus is transcriptionally repressed in response to the exogenous addition of heme. A high-copy suppressor screen for regulators of hem gene expression resulted in the identification of an LysR-type transcriptional regulator, called HbrL, that regulates hem promoters in response to the availability of heme. HbrL is shown to activate the expression of hemA and hemZ in the absence of exogenous hemin and repress hemB expression in the presence of exogenous hemin. Heterologously expressed HbrL apoprotein binds heme b and is purified with bound heme b when expressed in the presence of 5-aminolevulinic acid. Electrophoretic gel shift analysis demonstrated that HbrL binds the promoter region of hemA, hemB, and hemZ as well as its own promoter and that the presence of heme increases the binding affinity of HbrL to hemB.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Smart JL,Bauer CEdoi
10.1128/JB.188.4.1567-1576.2006keywords:
subject
Has Abstractpub_date
2006-02-01 00:00:00pages
1567-76issue
4eissn
0021-9193issn
1098-5530pii
188/4/1567journal_volume
188pub_type
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更新日期:1983-03-01 00:00:00
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更新日期:1976-01-01 00:00:00
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journal_title:Journal of bacteriology
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doi:10.1128/JB.91.5.1977-1985.1966
更新日期:1966-05-01 00:00:00
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更新日期:1972-02-01 00:00:00