Abstract:
:Lornitzo, Frank A. (Veterans Administration Hospital, Madison, Wis.), and Dexter S. Goldman. Reversible effect of bicarbonate on the inhibition of mycobacterial and yeast transglucosylases by mycoribnin. J. Bacteriol. 89:1086-1091. 1965.-The transglucosylase which catalyzes the formation of trehalose-6-phosphate from uridine diphosphate (UDP)-glucose and glucose-6-phosphate was purified from cell-free extracts of Mycobacterium tuberculosis H37Ra. During the purification procedure, the transglucosylase loses its sensitivity to mycoribnin, an inhibitor also found in these extracts. Sensitivity of the transglucosylase to mycoribnin is regained when the bicarbonate concentration of either enzyme or mycoribnin preparations is reduced to about 0.01 mm; sensitivity to mycoribnin is lost when low concentrations (< 1 mm) of bicarbonate are present in the reaction mixture. Transglucosylase preparations retain their bicarbonate-induced insensitivity to mycoribnin after dilution to a bicarbonate concentration which is ineffective for the initial conversion to insensitivity. The transglucosylases of brewer's yeast and of physiologically young (9-day) H37Ra cells, previously reported as insensitive to mycoribnin, have been partially purified. If bicarbonate is excluded from these preparations, the transglucosylases become sensitive to mycoribnin; bicarbonate abolishes this sensitivity. The H37Ra transglucosylase is specific for UDP-glucose and glucose-6-phosphate as substrates. UDP-galactose does not serve as a glycosyl donor; galactose-6-phosphate, ribose-5-phosphate, and glucose-1-phosphate do not act as glucosyl acceptors. Oligoribonucleotide analogues of mycoribnin do not inhibit substantially the H37Ra transglucosylase.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
LORNITZO FA,GOLDMAN DSdoi
10.1128/JB.89.4.1086-1091.1965keywords:
subject
Has Abstractpub_date
1965-04-01 00:00:00pages
1086-91eissn
0021-9193issn
1098-5530journal_volume
89pub_type
杂志文章abstract::The penicillin-binding proteins (PBPs) of 209 cell division (or growth) temperature-sensitive mutants of Streptococcus faecium were analyzed in this study. A total of nine strains showed either constitutive or temperature-sensitive conditional damage in the PBPs. Analysis of these nine strains yielded the following re...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.6.2432-2439.1987
更新日期:1987-06-01 00:00:00
abstract::The PmrA/PmrB two-component system encoded by the pmrCAB operon regulates the modification of Salmonella enterica serovar Typhimurium lipopolysaccharide leading to polymyxin B resistance. PmrA and PhoP are the only known activators of pmrCAB. A transposon mutagenesis screen for additional regulators of a pmrC::MudJ fu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.188.1.141-149.2006
更新日期:2006-01-01 00:00:00
abstract::The gene coding for sorbitol dehydrogenase (SDH) of Rhodobacter sphaeroides Si4 was located 55 nucleotides upstream of the mannitol dehydrogenase gene (mtlK) within a previously unrecognized polyol operon. This operon probably consists of all the proteins necessary for transport and metabolization of various polyols. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.20.6335-6340.1997
更新日期:1997-10-01 00:00:00
abstract::The product of the papD gene of uropathogenic Escherichia coli is required for the biogenesis of digalactoside-binding P pili. Mutations within papD result in complete degradation of the major pilus subunit, PapA, and of the pilinlike proteins PapE and PapF and also cause partial breakdown of the PapG adhesin. The pap...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.11.6052-6058.1989
更新日期:1989-11-01 00:00:00
abstract::Purified suspensions of Chlamydia psittaci were prepared from L cells. Thin sections of intact elementary bodies and intact developmental reticulate bodies and of their purified envelopes were observed by electron microscopy. In both intact organisms and partially purified envelopes, two membranous structures, each ap...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.105.1.355-360.1971
更新日期:1971-01-01 00:00:00
abstract::The motility of Spirillum volutans is caused by the rotation of each polar flagellar fascicle in a direction opposite to that of the more slowly rotating cell. Both flagella form cones of revolution oriented in the same direction. When the cell reverses its motion, both fascicles simultaneously reverse their rotation ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.94.5.1431-1436.1967
更新日期:1967-11-01 00:00:00
abstract::Edwards, P. R. (Communicable Disease Center, U.S. Public Health Service, Atlanta, Ga.), R. Sakazaki, and I. Kato. Natural occurrence of four reversible flagellar phases in cultures of Salmonella mikawashima. J. Bacteriol. 84:99-103. 1962-Study of cultures of Salmonella mikawashima isolated from reptiles revealed that ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.84.1.99-103.1962
更新日期:1962-07-01 00:00:00
abstract::Shah, D. B. (University of Wisconsin, Madison), and J. B. Wilson. Egg yolk factor of Staphylococcus aureus. II. Characterization of the lipase activity. J. Bacteriol. 89:949-953. 1965.-The staphylococcal egg yolk factor was characterized as a lipase. The enzyme had an optimal pH of 7.8, but the optimal pH of stability...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.89.4.949-953.1965
更新日期:1965-04-01 00:00:00
abstract::This work describes the draft genome sequence of Lactococcus garvieae DCC43. The 2.2-Mb draft genome contains 2,227 predicted protein-coding genes, among which is a region encoding the bacteriocin garvicin ML. No antibiotic resistance genes or capsule-related virulence genes were identified. Two plasmid replication re...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01864-12
更新日期:2012-12-01 00:00:00
abstract::Sinorhizobium meliloti strains lacking BacA function are impaired in symbiosis with alfalfa host plants and display altered sensitivities to a number of compounds relative to wild-type strains. With the goal of finding clues to the currently unknown biological function(s) of BacA, we carried out a genetic analysis to ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.21.6444-6453.2001
更新日期:2001-11-01 00:00:00
abstract::The Hly region from the chromosome of Vibrio cholerae El Tor strain RV79(Hly-) and the nonhemolytic classical strain 569B were cloned into plasmid vector pBR322. Escherichia coli K-12 transformants possessing these recombinant plasmids were nonhemolytic and were detected with a 32P-labeled hly-specific DNA probe. Rest...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.162.1.35-41.1985
更新日期:1985-04-01 00:00:00
abstract::The expression pattern of the Escherichia coli genome is controlled in part by regulating the utilization of a limited number of RNA polymerases among a total of its approximately 4,600 genes. The distribution pattern of RNA polymerase changes from modulation of two types of protein-protein interactions: the interacti...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01579-14
更新日期:2014-08-01 00:00:00
abstract::Expression of the Aspergillus nidulans penicillin biosynthesis genes acvA and ipnA, encoding delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase and isopenicillin N synthetase, respectively, was analyzed. The intergenic region carrying the divergently oriented promoters was fused in frame in both orientations ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.11.3789-3799.1992
更新日期:1992-06-01 00:00:00
abstract::In Rhodobacter sphaeroides, MreB, MreC, MreD, PBP2, and RodA are encoded at the same locus. The localizations of PBP2, MreB, and MreC, which have all been implicated in the synthesis of the peptidoglycan layer, were investigated under different growth conditions to gain insight into the relationships between these pro...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.188.5.1691-1700.2006
更新日期:2006-03-01 00:00:00
abstract::The sheath of the filamentous, gliding cyanobacterium Phormidium uncinatum was studied by using light and electron microscopy. In thin sections and freeze fractures the sheath was found to be composed of helically arranged carbohydrate fibrils, 4 to 7 nm in diameter, which showed a substantial degree of crystallinity....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.15.3923-3932.1998
更新日期:1998-08-01 00:00:00
abstract::The Bacillus subtilis ferric uptake regulator (Fur) protein is the major sensor of cellular iron status. When iron is limiting for growth, derepression of the Fur regulon increases the cellular capacity for iron uptake and mobilizes an iron-sparing response mediated in large part by a small noncoding RNA named FsrA. F...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.05990-11
更新日期:2012-05-01 00:00:00
abstract::Herbaspirillum sp. strain GW103 was isolated from rhizosphere soil of the reed Phragmites australis on reclaimed land. Here we report the 5.05-Mb draft genome sequence of the strain, providing bioinformation about the agronomic benefits of this strain, such as multiple traits relevant to plant root colonization and pl...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00806-12
更新日期:2012-08-01 00:00:00
abstract::Although yeast cannot normally incorporate exogenous deoxythymidine 5'-monophosphate (dTMP) into deoxyribonucleic acid, mutants able to do so have been isolated. We have characterized a recessive suppressor of dTMP uptake (sot1) that prevents strains carrying either tup1, tup2, or tup4 from growing on selective medium...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.138.2.638-641.1979
更新日期:1979-05-01 00:00:00
abstract::Western immunoblots and assays of Bacillus subtilis extracts showed that intracellular serine protease 1 is produced in a form larger than previously reported, appears not to have undergone N-terminal processing, and is active in the presence or absence of calcium. No evidence for an inactive precursor form of the pro...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.1.473-476.1990
更新日期:1990-01-01 00:00:00
abstract::The nucleoids in Mycoplasma capricolum cells were visualized by phase-combined fluorescence microscopy of DAPI (4', 6-diamidino-2-phenylindole)-stained cells. Most growing cells in a rich medium had one or two nucleoids in a cell, and no anucleate cells were found. The nucleoids were positioned in the center in mononu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.19.6073-6080.1999
更新日期:1999-10-01 00:00:00
abstract::An analysis of the turnover of the major phospholipids of Micrococcus cerificans growing or nongrowing cultures. The turnover rates of (14)C-PE and (14)C-PE were 61.5% of the total phospholipid, exhibited no significant rate of turnover in either growing or nongrowing cultures. The turnover rates of PE-(14)C and PE-(3...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.107.3.806-814.1971
更新日期:1971-09-01 00:00:00
abstract::The fim genetic switch in the chromosome of Escherichia coli K-12 is an invertible DNA element that harbors the promoter for transcription of the downstream fim structural genes and a transcription terminator that acts on the upstream fimE regulatory gene. Switches oriented appropriately for structural gene transcript...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.24.8256-8266.2005
更新日期:2005-12-01 00:00:00
abstract::The fine structure of Paracoccidioides brasiliensis undergoing temperature-dependent transformation from mycelium to yeast and vice versa (M right harpoon over left harpoon Y) was studied. The transitional form to mycelium from the yeast appears as an elongated bud that extends from the yeast and which has a mixture o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.100.2.1076-1082.1969
更新日期:1969-11-01 00:00:00
abstract::A sulfonic acid found to be a major constituent of spores of Bacillus subtilis was provisionally identified as 3-l-sulfolactic acid. This compound was completely absent from vegetative cells during growth, but large amounts accumulated in sporulating cells just before the development of refractile spores. Essentially ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.98.1.62-68.1969
更新日期:1969-04-01 00:00:00
abstract::The kinetics of chromosome replication and cell division have been examined in recA mutants of Escherichia coli B/r containing F' plasmids of various sizes. Plasmid-mediated alterations in growth properties were detected only with the presence of the larger F' plasmids, and were reflected in decreased mean cell sizes ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.137.3.1151-1157.1979
更新日期:1979-03-01 00:00:00
abstract::Plasmid pL32 from the Natto strain of Bacillus subtilis belongs to a group of low-copy-number plasmids in gram-positive bacteria that replicate via a theta mechanism of replication. We studied the DNA region encoding the replication protein, RepN, of pLS32, and obtained the following results. Transcription of the repN...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.13.4315-4326.2005
更新日期:2005-07-01 00:00:00
abstract::Whole-genome sequence analysis of Mycobacterium leprae has revealed a limited number of protein-coding genes, with half of the genome composed of pseudogenes and noncoding regions. We previously showed that some M. leprae pseudogenes are transcribed at high levels and that their expression levels change following infe...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00120-09
更新日期:2009-05-01 00:00:00
abstract::The Gifsy-1 phage integrates site specifically into the Salmonella chromosome via an integrase-mediated site-specific recombination mechanism. Initial genetic analysis suggests that Gifsy-1 integrase-mediated excision of the Gifsy-1 phage is influenced by proteins encoded by both the Gifsy-1 and the Gifsy-2 phages. Ou...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00577-07
更新日期:2007-09-01 00:00:00
abstract::A colorimetric assay was developed for studying the kinetics of iron oxidation with whole cells of the chemoautotroph, Ferrobacillus ferrooxidans. The assay was more advantageous than the conventional method of Warburg manometry because of its simplicity, rapidity, and the small amount of cells required. The assay mea...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.99.2.552-557.1969
更新日期:1969-08-01 00:00:00
abstract::The content and distribution of autolysin were measured in temperature-sensitive morphological mutants of Bacillus subtilis. Strains RUB1000 and RUB1012 grew as rods at 30 C. At 45 C the mutants contained disproportionately less teichoic acid than peptidoglycan and grew as irregular spheres. The amount of enzyme that ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.125.1.166-173.1976
更新日期:1976-01-01 00:00:00