Mutations in residues involved in zinc binding in the catalytic site of Escherichia coli threonyl-tRNA synthetase confer a dominant lethal phenotype.

abstract：:In order to examine the potential role of bacterial collagenases in periodontal tissue destruction, we recently isolated a gene, prtC, from Porphyromonas gingivalis ATCC 53977, which expressed collagenase activity (N. Takahashi, T. Kato, and H. K. Kuramitsu, FEMS Microbiol. Lett. 84:135-138, 1991). The nucleotide sequ...

journal_title：Journal of bacteriology

authors： Kato T,Takahashi N,Kuramitsu HK

更新日期：1992-06-01 00:00:00

abstract：:Apparent pK values were determined for a series of commercially available carboxylic acids in a search for a buffer suitable for bacterial studies at pH 7 in media free from nitrogen, sulfur, and phosphorus. Of the compounds possessing pK' values near pH 7,3,6-endomethylene-1,2,3,6-tetrahydrophthalic acid (EMTA) was c...

journal_title：Journal of bacteriology

authors： Mallette MF

更新日期：1967-08-01 00:00:00

abstract：:Recently we identified a bacterial factor (BimA) required for actin-based motility of Burkholderia pseudomallei. Here we report that Burkholderia mallei and Burkholderia thailandensis are capable of actin-based motility in J774.2 cells and that BimA homologs of these bacteria can restore the actin-based motility defec...

journal_title：Journal of bacteriology

authors： Stevens JM,Ulrich RL,Taylor LA,Wood MW,Deshazer D,Stevens MP,Galyov EE

更新日期：2005-11-01 00:00:00

abstract：:d-Arabinose isomerase (EC 5.3.1.3) has been isolated from l-fucose-induced cultures of Escherichia coli K-12 and d-arabinose-induced cultures of E. coli B/r. Both enzymes were homogeneous in an ultracentrifuge and migrated as single bands upon disc electrophoresis in acrylamide gels. The s(20,w) was 14.5 x 10(-13) sec...

journal_title：Journal of bacteriology

authors： Boulter JR,Gielow WO

更新日期：1973-02-01 00:00:00

abstract：:The azlB locus of Bacillus subtilis was defined previously by a mutation conferring resistance to a leucine analog, 4-azaleucine (J. B. Ward, Jr., and S. A. Zahler, J. Bacteriol. 116:727-735, 1973). In this report, azlB is shown to be the first gene of an operon apparently involved in branched-chain amino acid transpo...

journal_title：Journal of bacteriology

authors： Belitsky BR,Gustafsson MC,Sonenshein AL,Von Wachenfeldt C

更新日期：1997-09-01 00:00:00

abstract：:Clostridium perfringens type B and D isolates produce epsilon-toxin, the third most potent clostridial toxin. The epsilon-toxin gene (etx) is plasmid borne in type D isolates, but etx genetics have been poorly studied in type B isolates. This study reports the first sequencing of any etx plasmid, i.e., pCP8533etx, fro...

journal_title：Journal of bacteriology

authors： Miyamoto K,Li J,Sayeed S,Akimoto S,McClane BA

更新日期：2008-11-01 00:00:00

abstract：:2-Aminonumconic 6-semialdehyde is an unstable intermediate in the biodegradation of nitrobenzene and 2-aminophenol by Pseudomonas pseudoalcaligenes JS45. Previous work has shown that enzymes in cell extracts convert 2-aminophenol to 2-aminomuconate in the presence of NAD+. In the present work, 2-aminomuconic semialdeh...

journal_title：Journal of bacteriology

authors： He Z,Davis JK,Spain JC

更新日期：1998-09-01 00:00:00

abstract：:The D1:1 protein and its potentially occurring degradation products were overexpressed in Escherichia coli. Protein-DNA interaction is shown for the promoter region of psbAI. The D1:1 degradation products may be involved in transcription regulation of psbAI by binding in the promoter region. Additionally, C-terminal f...

journal_title：Journal of bacteriology

authors： Stelljes C,Koenig F

更新日期：2007-03-01 00:00:00

abstract：:A population of Bacillus subtilis can be fractionated into at least two components on the basis of their buoyant densities on a Renografin gradient. In competent cultures, most, and perhaps all, of the competent bacteria appear in the lighter fraction which composes 2 to 10% of the bulk population. These "lighter" bac...

journal_title：Journal of bacteriology

authors： Cahn FH,Fox MS

更新日期：1968-03-01 00:00:00

abstract：:Filaments of the heterocyst-forming cyanobacterium Anabaena variabilis reproduced 35SO4(2)-, incorporating 35S into cysteine, methionine, glutathione, sulfolipid, and several unidentified metabolites. The majority of the incorporated label accumulated in reduced glutathione. Heterocysts isolated from labeled filaments...

journal_title：Journal of bacteriology

authors： Giddings TH Jr,Wolk CP,Shomer-Ilan A

更新日期：1981-06-01 00:00:00

abstract：:Integrons are genetic elements that contribute to lateral gene transfer in bacteria as a consequence of possessing a site-specific recombination system. This system facilitates the spread of genes when they are part of mobile cassettes. Most integrons are contained within chromosomes and are confined to specific bacte...

journal_title：Journal of bacteriology

authors： Stokes HW,Nesbø CL,Holley M,Bahl MI,Gillings MR,Boucher Y

更新日期：2006-08-01 00:00:00

abstract：:The penicillin-binding proteins (PBPs) of 209 cell division (or growth) temperature-sensitive mutants of Streptococcus faecium were analyzed in this study. A total of nine strains showed either constitutive or temperature-sensitive conditional damage in the PBPs. Analysis of these nine strains yielded the following re...

journal_title：Journal of bacteriology

authors： Canepari P,Lleò MM,Fontana R,Satta G

更新日期：1987-06-01 00:00:00

abstract：:Desulfovibrio vulgaris Hildenborough, a sulfate-reducing bacterium classified as an obligate anaerobe, swam to a preferred oxygen concentration of 0.02 to 0.04% (0.24 to 0.48 microM), a level which also supported growth. Oxygen concentrations of 0.08% and higher arrested growth. We propose that in zones of transition ...

journal_title：Journal of bacteriology

authors： Johnson MS,Zhulin IB,Gapuzan ME,Taylor BL

更新日期：1997-09-01 00:00:00

abstract：:Protocatechuate 3,4-dioxygenase is a member of a family of bacterial enzymes that cleave the aromatic rings of their substrates between two adjacent hydroxyl groups, a key reaction in microbial metabolism of varied environmental chemicals. In an appropriate genetic background, it is possible to select for Acinetobacte...

journal_title：Journal of bacteriology

authors： D'Argenio DA,Vetting MW,Ohlendorf DH,Ornston LN

更新日期：1999-10-01 00:00:00

abstract：:Phase-contrast and serial-section electron microscopy were used to study the patterns of localized plasmolysis that occur when cells of Salmonella typhimurium and Escherichia coli are exposed to hypertonic solutions of sucrose. In dividing cells the nascent septum was flanked by localized regions of periseptal plasmol...

journal_title：Journal of bacteriology

authors： Cook WR,MacAlister TJ,Rothfield LI

更新日期：1986-12-01 00:00:00

abstract：:The opportunistic pathogen Pseudomonas aeruginosa expresses polar type IV pili (TFP), which are responsible for adhesion to various materials and twitching motility on surfaces. Twitching occurs by alternate extension and retraction of TFP, which arise from assembly and disassembly of pilin subunits at the base of the...

journal_title：Journal of bacteriology

authors： Chiang P,Habash M,Burrows LL

更新日期：2005-02-01 00:00:00

abstract：:The oral cavity is home to a wide variety of bacterial species, both commensal, such as various streptococcal species, and pathogenic, such as Porphyromonas gingivalis, one of the main etiological agents of periodontal disease. Our understanding of how these bacteria ultimately cause disease is highly dependent upon u...

journal_title：Journal of bacteriology

authors： Rocco CJ,Bakaletz LO,Goodman SD

更新日期：2018-05-09 00:00:00

abstract：:A series of R (rough) Salmonella minnesota mutants with rfb, rfe, and rfa mutations leading to various defects in the biosynthesis of cell wall lipopolysaccharide was analyzed as to their enterobacterial common antigen (CA) content. All mutants that had functional rfe genes were CA(+) as is the wild-type parent. This ...

journal_title：Journal of bacteriology

authors： Mäkelä PH,Mayer H,Whang HY,Neter E

更新日期：1974-09-01 00:00:00

abstract：:Protein and mRNA levels of heat-labile enterotoxin (LT) of Escherichia coli are highest at 37 degrees C, and they decrease gradually as temperature is decreased. This temperature effect is eliminated in an Hns- mutant. Deletion of portions of DNA coding for the LT A subunit also results in an increase in LT expression...

journal_title：Journal of bacteriology

authors： Trachman JD,Maas WK

更新日期：1998-07-01 00:00:00

abstract：:Methicillin resistance in staphylococci is mediated by PBP2a, a penicillin binding protein with low affinity for beta-lactam antibiotics. The gene encoding PBP2a, mecA, is transcriptionally regulated in some clinical isolates by mecR1 and mecI, genes divergently transcribed from mecA that encode a signal transducer an...

journal_title：Journal of bacteriology

authors： Sharma VK,Hackbarth CJ,Dickinson TM,Archer GL

更新日期：1998-04-01 00:00:00

abstract：:A DNA sequence (brpA) which regulates the expression of the genes of the bialaphos biosynthesis pathway (bap) in Streptomyces hygroscopicus was identified and characterized. A newly isolated nonproducing mutant (NP57) had a pleiotropic defect involving at least 6 of the 13 known bap genes; only the step 6 conversion c...

journal_title：Journal of bacteriology

authors： Anzai H,Murakami T,Imai S,Satoh A,Nagaoka K,Thompson CJ

更新日期：1987-08-01 00:00:00

abstract：:Histidine decarboxylases from Klebsiella planticola and Enterobacter aerogenes were purified to homogeneity and compared with the histidine decarboxylase from Morganella morganii. All three enzymes required pyridoxal 5'-phosphate as a coenzyme, showed optimal activity at pH 6.5, decarboxylated only histidine among the...

journal_title：Journal of bacteriology

authors： Guirard BM,Snell EE

更新日期：1987-09-01 00:00:00

abstract：:Mutants of Anabaena sp. strain PCC 7120 that are incapable of sustained growth with air as the sole source of nitrogen were generated by using Tn5-derived transposons. Nitrogenase was expressed only in mutants that showed obvious morphological signs of heterocyst differentiation. Even under rigorously anaerobic condit...

journal_title：Journal of bacteriology

authors： Ernst A,Black T,Cai Y,Panoff JM,Tiwari DN,Wolk CP

更新日期：1992-10-01 00:00:00

abstract：:To determine whether homologous recombination could be used to inactivate selected genes in Spiroplasma citri, plasmid constructs were designed to disrupt the motility gene scm1. An internal scm1 gene fragment was inserted into plasmid pKT1, which replicates in Escherichia coli but not in S. citri, and into the S. cit...

journal_title：Journal of bacteriology

authors： Duret S,Danet JL,Garnier M,Renaudin J

更新日期：1999-12-01 00:00:00

abstract：:Groman, Neal B. (University of Washington, Seattle) and Grace Suzuki. Quantitative study of endolysin synthesis during reproduction of lambda phages. J. Bacteriol. 86:187-194. 1963.-Endolysin is presumed to be a phage-induced enzyme participating in lysis through its destructive action on the host cell wall. A method ...

journal_title：Journal of bacteriology

authors： GROMAN NB,SUZUKI G

更新日期：1963-08-01 00:00:00

abstract：:Vibrio parahaemolyticus possesses dual flagellar systems adapted for movement under different circumstances. A single polar flagellum propels the bacterium in liquid (i.e., swimming) with a motor that is powered by the sodium motive force. Multiple proton-driven lateral flagella enable translocation over surfaces (i.e...

journal_title：Journal of bacteriology

authors： Stewart BJ,McCarter LL

更新日期：2003-08-01 00:00:00

abstract：:Acetyl coenzyme A synthetase (Acs) activates acetate to acetyl coenzyme A through an acetyladenylate intermediate; two other enzymes, acetate kinase (Ack) and phosphotransacetylase (Pta), activate acetate through an acetyl phosphate intermediate. We subcloned acs, the Escherichia coli open reading frame purported to e...

journal_title：Journal of bacteriology

authors： Kumari S,Tishel R,Eisenbach M,Wolfe AJ

更新日期：1995-05-01 00:00:00

abstract：:The extracytoplasmic factor (ECF) sigma factor sigma(E) is one of the most studied sigma factors of Mycobacterium tuberculosis. It has been shown to be involved in virulence as well as in survival under conditions of high temperature, alkaline pH, and exposure to detergents and oxidative stress. Unlike many ECF sigma ...

journal_title：Journal of bacteriology

authors： Donà V,Rodrigue S,Dainese E,Palù G,Gaudreau L,Manganelli R,Provvedi R

更新日期：2008-09-01 00:00:00

abstract：:Wiebers, Joyce L. (Purdue University, West Lafayette, Ind.), and Harold R. Garner. Use of S-methylcysteine and cystathionine by methionineless Neurospora mutants. J. Bacteriol. 88:1798-1804. 1964.-Radioactive methionine was found in hydrolysates of various strains of Neurospora crassa when either S-methylcysteine (SMC...

journal_title：Journal of bacteriology

authors： WIEBERS JL,GARNER HR

更新日期：1964-12-01 00:00:00

abstract：:Freeman, Bob A. (University of Chicago, Chicago, Ill.) and Richard Circo. Effect of tetracyclines on the intracellular amino acids of molds. J. Bacteriol. 86:38-44. 1963.-The tetracycline antibiotics were shown to alter the amino acid metabolism of molds whose growth is not markedly affected. Eight molds were grown in...

journal_title：Journal of bacteriology

authors： FREEMAN BA,CIRCO R

更新日期：1963-07-01 00:00:00