Abstract:
:Examination of the ilvF locus at 54 min on the Escherichia coli K-12 chromosome revealed that it is a cryptic gene for expression of a valine-resistant acetohydroxy acid synthase (acetolactate synthase; EC 4.1.3.18) distinct from previously reported isozymes. A spontaneous mutation, ilvF663, yielded IlvF+ enzyme activity that was multivalently repressed by all three branched-chain amino acids, was completely insensitive to feedback inhibition, was highly stable at elevated temperatures, and expressed optimal activity at 50 degrees C. The IlvF+ enzyme activity was expressed in strains in which isozyme II was inactive because of the ilvG frameshift in the wild-type strain K-12 and isozymes I and III were inactivated by point mutations or deletions. Tn5 insertional mutagenesis yielded two IlvF- mutants, with the insertion in ilvF663 in each case. These observations suggest that the ilvF663 locus may be a coding region for a unique acetohydroxy acid synthase activity.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Alexander-Caudle C,Latinwo LM,Jackson JHdoi
10.1128/jb.172.6.3060-3065.1990subject
Has Abstractpub_date
1990-06-01 00:00:00pages
3060-5issue
6eissn
0021-9193issn
1098-5530journal_volume
172pub_type
杂志文章abstract::During batch growth of Alcaligenes eutrophus on benzoate-acetate mixtures, benzoate was the preferred substrate, with acetate consumption being delayed until the rate of benzoate consumption had diminished. This effect was attributed to a transcriptional control of the synthesis of acetyl coenzyme A (acetyl-CoA) synth...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.20.5826-5833.1995
更新日期:1995-10-01 00:00:00
abstract::The gammaproteobacterium Shewanella oneidensis MR-1 utilizes a complex electron transfer network composed primarily of c-type cytochromes to respire under anoxic conditions a variety of compounds, including fumarate, nitrate, and dimethyl sulfoxide (DMSO), in addition to the minerals Fe(III) and Mn(IV). Central to sev...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01355-10
更新日期:2011-05-01 00:00:00
abstract::Purified bulk tRNA from Methanococcus vanielii (carbon source, formate) showed variation in the modified nucleoside pattern reported for Escherichia coli as analyzed by both ion-exchange and thin-layer chromatography. Ribothymidine and 7-methylguanosine were absent; 1-methyladenosine, 1-methylguanosine, N2-methylguano...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.133.1.240-250.1978
更新日期:1978-01-01 00:00:00
abstract::The Escherichia coli htrD gene was originally isolated during a search for new genes required for growth at high temperature. Insertional inactivation of htrD leads to a pleiotropic phenotype characterized by temperature-sensitive growth in rich medium, H2O2 sensitivity, and sensitivity to cysteine. The htrD gene was ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.1.166-175.1993
更新日期:1993-01-01 00:00:00
abstract::Sbarra, Anthony J. (St. Margaret's Hospital, Boston, Mass.), William Shirley, and John S. Baumstark. Effect of osmolarity on phagocytosis. J. Bacteriol. 85:306-313. 1963.-The effect of a number of different compounds on phagocytosis was studied. Phagocytosis was monitored by morphological and biochemical means. It was...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.85.2.306-313.1963
更新日期:1963-02-01 00:00:00
abstract::The propanediol utilization (pdu) operon of Salmonella typhimurium encodes proteins required for the catabolism of propanediol, including a coenzyme B12-dependent propanediol dehydratase. A clone that expresses propanediol dehydratase activity was isolated from a Salmonella genomic library. DNA sequence analysis showe...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.21.6633-6639.1997
更新日期:1997-11-01 00:00:00
abstract::An examination of the methanofurans isolated from a wide range of methanogenic bacteria and from Archaeoglobus fulgidus has revealed at least five chromatographically distinct methanofurans. Bacteria from each major genus of methanogenic bacteria have been found to contain a chemically different methanofuran. The natu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.10.4594-4597.1988
更新日期:1988-10-01 00:00:00
abstract::In Bacillus subtilis the citM gene encodes the Mg(2+)-citrate transporter. A target site for carbon catabolite repression (cre site) is located upstream of citM. Fusions of the citM promoter region, including the cre sequence, to the beta-galactosidase reporter gene were constructed and integrated into the amyE site o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.21.6099-6105.2000
更新日期:2000-11-01 00:00:00
abstract::Groman, Neal B. (University of Washington, Seattle) and Grace Suzuki. Quantitative study of endolysin synthesis during reproduction of lambda phages. J. Bacteriol. 86:187-194. 1963.-Endolysin is presumed to be a phage-induced enzyme participating in lysis through its destructive action on the host cell wall. A method ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.86.2.187-194.1963
更新日期:1963-08-01 00:00:00
abstract::The DNA sequence of part of the gnd (6-phosphogluconate dehydrogenase) gene was determined for eight wild strains of Escherichia coli and for Salmonella typhimurium. Since a region of the trp (tryptophan) operon and the phoA (alkaline phosphatase) gene have been sequenced from the same strains, the gene trees for thes...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.22.7257-7268.1991
更新日期:1991-11-01 00:00:00
abstract::The predominant pool of organic matter on earth is derived from the biological reduction and assimilation of carbon dioxide gas, catalyzed primarily by the enzyme ribulose 1,5-bisphosphate carboxylase/oxygenase (RubisCO). By virtue of its capacity to use molecular oxygen as an alternative and competing gaseous substra...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.5.1569-1575.1999
更新日期:1999-03-01 00:00:00
abstract::Respiratory nitrate reductase purified from the cell membrane of Escherichia coli is composed of three subunits, alpha, beta, and gamma, which are encoded, respectively, by the narG, narH, and narI genes of the narGHJI operon. The product of the narJ gene was deduced previously to be a highly charged, acidic protein w...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.3.867-872.1992
更新日期:1992-02-01 00:00:00
abstract::We have isolated three strains of Klebsiella aerogenes that failed to show repression of glutamine synthetase even when grown under the most repressing conditions for the wild-type strain. These mutant strains were selected as glutamine-independent derivatives of a strain that is merodiploid for the glnA region and co...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.141.2.671-679.1980
更新日期:1980-02-01 00:00:00
abstract::The A-subunit polypeptides of Shiga toxin, the Shiga-like toxins (SLTs), and the plant lectin ricin inactivate eucaryotic ribosomes by enzymatically depurinating 28S rRNA. Comparison of the amino acid sequences of the members of the Shiga toxin family and ricin revealed two regions of significant homology that lie wit...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.6.3346-3350.1990
更新日期:1990-06-01 00:00:00
abstract::spo0A and spo0H are needed for the initiation of sporulation and for the development of genetic competence in Bacillus subtilis. Transcription of spo0A initiates from two promoters, Pv and Ps. Pv is active during vegetative growth and is recognized by RNA polymerase containing sigma A. Expression from Ps increases dur...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.12.3812-3815.1994
更新日期:1994-06-01 00:00:00
abstract::Late log-phase Escherichia coli B/r cells are 1.6 times more sensitive to killing by X rays than are stationary-phase cells when grown in Brain Heart Infusion (BHI) + glucose. The number of single-chain breaks formed per krad is the same for log- and stationary-phase cells. Stationary-phase cells show a somewhat great...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.105.1.127-135.1971
更新日期:1971-01-01 00:00:00
abstract::Escherichia coli mutants with improved organic solvent tolerance levels showed high levels of outer membrane protein TolC and inner membrane protein AcrA. The TolC level was regulated positively by MarA, Rob, or SoxS. A possible mar-rob-sox box sequence was found upstream of the tolC gene. These findings suggest that ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.4.938-944.1998
更新日期:1998-02-01 00:00:00
abstract::Beggs, William H. (University of Minnesota, Minneapolis), and Palmer Rogers. Galactose repression of beta-galactosidase induction in Escherichia coli. J. Bacteriol. 91:1869-1874. 1966.-Galactose repression of beta-galactosidase induction in Escherichia coli was investigated to determine whether the galactose molecule ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.91.5.1869-1874.1966
更新日期:1966-05-01 00:00:00
abstract::Staphylococcus aureus was shown to transport iron complexed to a variety of hydroxamate type siderophores, including ferrichrome, aerobactin, and desferrioxamine. An S. aureus mutant defective in the ability to transport ferric hydroxamate complexes was isolated from a Tn917-LTV1 transposon insertion library after sel...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.16.4394-4400.2000
更新日期:2000-08-01 00:00:00
abstract::Synthetic analogs of enterochelin (enterobactin) were tested for their ability to support the growth of Escherichia coli K-12 under iron-limiting conditions. The cyclic compound MECAM [1,3,5-N.N'; N"-tris-(2,3-dihydroxybenzoyl)-triamino-methylbenzene] and its N-methyl derivative Me3MECAM promoted growth, whereas the 2...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.153.1.109-115.1983
更新日期:1983-01-01 00:00:00
abstract::Hydrolytic deamination of cytosine to uracil in DNA is increased in organisms adapted to high temperatures. Hitherto, the uracil base excision repair (BER) pathway has only been described in two archaeons, the crenarchaeon Pyrobaculum aerophilum and the euryarchaeon Archaeoglobus fulgidus, which are hyperthermophiles ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00233-11
更新日期:2011-09-01 00:00:00
abstract::During meiosis and sporulation in Saccharomyces cerevisiae, the recessive genetic marker for cycloheximide resistance, believed to be due to an altered ribosomal protein (C. S. McLaughlin, p. 815-827, in M. Nomura et al., ed., Ribosomes, Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.), is expressed as early a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.145.3.1342-1350.1981
更新日期:1981-03-01 00:00:00
abstract::Frampton, E. W. (The University of Texas M. D. Anderson Hospital and Tumor Institute, Houston), and B. R. Brinkley. Evidence of lysogeny in derivatives of Escherichia coli. J. Bacteriol. 90:446-452. 1965.-Exposure of cultures of several thymine-requiring derivatives of Escherichia coli strain 15 to radiation [X rays o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.90.2.446-452.1965
更新日期:1965-08-01 00:00:00
abstract::In this communication we present evidence that indicates that 1,2-propanediol (1,2-PDL) is a positive effector of the transcription of the cobalamin (vitamin B12) biosynthetic (cob) operon and of the 1,2-PDL utilization (pdu) genes. The stimulatory effects of 1,2-PDL were demonstrated using Mu d-lac transcriptional fu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.7.2267-2272.1992
更新日期:1992-04-01 00:00:00
abstract::Purified superdormant spores of Bacillus cereus, B. megaterium, and B. subtilis isolated after optimal heat activation of dormant spores and subsequent germination with inosine, d-glucose, or l-valine, respectively, germinate very poorly with the original germinants used to remove dormant spores from spore populations...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00736-09
更新日期:2009-09-01 00:00:00
abstract:UNLABELLED:Our laboratory recently discovered that Escherichia coli cells starved for the DNA precursor dGTP are killed efficiently (dGTP starvation) in a manner similar to that described for thymineless death (TLD). Conditions for specific dGTP starvation can be achieved by depriving an E. coli optA1 gpt strain of the...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00218-16
更新日期:2016-05-13 00:00:00
abstract::The histidine ATP-binding cassette (ABC) transporter of Salmonella enterica serovar Typhimurium is among the best-studied type I ABC import systems. The transporter consists of two transmembrane subunits, HisQ and HisM, and a homodimer of the nucleotide-binding subunit, HisP. Substrates are delivered by two periplasmi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00521-18
更新日期:2018-12-20 00:00:00
abstract::FtsK is essential for Escherichia coli cell division. We report that cells lacking the C terminus of FtsK are defective in chromosome segregation as well as septation, often exhibiting asymmetrically positioned nucleoids and large anucleate regions. Combining the corresponding truncated ftsK gene with a mukB null muta...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:
更新日期:1998-12-01 00:00:00
abstract::The initiation process of deoxyribonucleic acid (DNA) replication in Escherichia coli has been studied using the thermoreversible dna initiation mutant E. coli HfrHl65/120/6 dna-252. This dna mutation was incorrectly classed as a dnaA mutation. Biochemical and genetic evidence suggests that the dna-252 mutant is a nov...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.129.3.1476-1486.1977
更新日期:1977-03-01 00:00:00
abstract::Strain DCB-1 is a strict anaerobe capable of reductive dehalogenation. We elucidated metabolic processes in DCB-1 which may be related to dehalogenation and which further characterize the organism physiologically. Sulfoxy anions and CO2 were used by DCB-1 as catabolic electron acceptors. With suitable electron donors,...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.4.2065-2070.1990
更新日期:1990-04-01 00:00:00