Abstract:
:SufA, IscA, and Nfu have been proposed to function as scaffolds in the assembly of Fe/S clusters in bacteria. To investigate the roles of these proteins further, single and double null-mutant strains of Synechococcus sp. strain PCC 7002 were constructed by insertional inactivation of genes homologous to sufA, iscA, and nfu. Demonstrating the nonessential nature of their products, the sufA, iscA, and sufA iscA mutants grew photoautotrophically with doubling times that were similar to the wild type under standard growth conditions. In contrast, attempts to inactivate the nfu gene only resulted in stable merodiploids. These results imply that Nfu, but not SufA or IscA, is the essential Fe/S scaffold protein in cyanobacteria. When cells were grown under iron-limiting conditions, the iscA and sufA mutant strains exhibited less chlorosis than the wild type. Under iron-sufficient growth conditions, isiA transcript levels, a marker for iron limitation in cyanobacteria, as well as transcript levels of genes in both the suf and isc regulons were significantly higher in the iscA mutant than in the wild type. Under photosynthesis-induced redox stress conditions, the transcript levels of the suf genes are notably higher in the sufA and the sufA iscA mutants than in the wild type. The growth phenotypes and mRNA abundance patterns of the mutant strains contradict the proposed scaffold function for the SufA and IscA proteins in generalized Fe/S cluster assembly and instead suggest that they play regulatory roles in iron homeostasis and the sensing of redox stress in cyanobacteria.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Balasubramanian R,Shen G,Bryant DA,Golbeck JHdoi
10.1128/JB.188.9.3182-3191.2006subject
Has Abstractpub_date
2006-05-01 00:00:00pages
3182-91issue
9eissn
0021-9193issn
1098-5530pii
188/9/3182journal_volume
188pub_type
杂志文章abstract::Somerson, Norman L. (National Institute of Allergy and Infectious Diseases, Bethesda, Md.), and M. K. Cook. Suppression of Rous sarcoma virus growth in tissue cultures by Mycoplasma orale. J. Bacteriol. 90:534-540. 1965.-An agent which produced cell destruction in human diploid and chick-embryo fibroblasts was isolate...
journal_title:Journal of bacteriology
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doi:10.1128/JB.90.2.534-540.1965
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abstract::The two-partner secretion system ExlBA, expressed by strains of Pseudomonas aeruginosa belonging to the PA7 group, induces hemorrhage in lungs due to disruption of host cellular membranes. Here we demonstrate that the exlBA genes are controlled by a pathway consisting of cAMP and the virulence factor regulator (Vfr). ...
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
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pub_type: 杂志文章
doi:10.1128/jb.174.13.4416-4426.1992
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abstract::Davis, Nour A. (University of Lagos Medical School, Lagos, Nigeria), and G. H. G. Davis. Ecology of nasal staphylococci. J. Bacteriol. 89:1163-1168. 1965.-The rate of nasal carriage of Staphylococcus aureus in Nigerian adults (46%) approximates that found in other countries. The rate in infants under 12 months was ca....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.89.5.1163-1168.1965
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.5.2245-2250.1987
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journal_title:Journal of bacteriology
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pub_type: 杂志文章
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更新日期:1993-03-01 00:00:00
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更新日期:1992-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1986-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2016-05-13 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1977-02-01 00:00:00
abstract::CTXphi is a filamentous bacteriophage that encodes cholera toxin. CTXphi infection of its host bacterium, Vibrio cholerae, requires the toxin-coregulated pilus (TCP) and the products of the V. cholerae tolQRA genes. Here, we have explored the role of OrfU, a predicted CTXphi minor coat protein, in CTXphi infection. Pr...
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pub_type: 杂志文章
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更新日期:2003-02-01 00:00:00