The Streptomyces glaucescens TcmR protein represses transcription of the divergently oriented tcmR and tcmA genes by binding to an intergenic operator region.

Abstract:

:Preliminary evidence has been presented by Guilfoile and Hutchinson (J. Bacteriol. 174:3651-3658, 1992) suggesting that the Streptomyces glaucescens TcmR protein is a transcriptional repressor. Here, we extend that work by showing that transcription of the S. glaucescens tcmA gene is inducible by tetracenomycin C and that inactivation of the tcmR gene results in constitutive transcription of the tcmA gene. Gel retardation studies show that the TcmR protein binds to the tcmA-tcmR intergenic region in vitro and that this binding is inhibited by tetracenomycin C. Footprinting experiments demonstrate that the TcmR protein binds to an operator region that encompasses both the tcmA and the tcmR promoters. This genetic and biochemical evidence strongly supports the model of the TcmR protein acting as a repressor in inhibiting transcription of both the tcmA and the tcmR genes, in much the same way that TetR from Tn10 inhibits transcription of tetA and tetR.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Guilfoile PG,Hutchinson CR

doi

10.1128/jb.174.11.3659-3666.1992

keywords:

subject

Has Abstract

pub_date

1992-06-01 00:00:00

pages

3659-66

issue

11

eissn

0021-9193

issn

1098-5530

journal_volume

174

pub_type

杂志文章
  • Characterization of the membrane-bound succinic dehydrogenase of Micrococcus lysodeikticus.

    abstract::The occurrence of succinic dehydrogenase [succinic:(acceptor) oxidoreductase, EC 1.3.99.1] in membrane fractions of Micrococcus lysodeikticus was investigated. The enzyme could be purified 10-fold, by deoxycholate treatment. Butanol extraction of membranes yielded an active fraction, nonsedimentable at 130,000 x g for...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.107.1.230-238.1971

    authors: Pollock JJ,Linder R,Salton MR

    更新日期:1971-07-01 00:00:00

  • Evidence that TP_0144 of Treponema pallidum is a thiamine-binding protein.

    abstract::Thiamine pyrophosphate (TPP), the biologically active form of thiamine (also known as vitamin B1), is an essential cofactor for several important enzymes involved in carbohydrate metabolism, and therefore, it is required for all living organisms. We recently found that a thiamine-binding protein (TDE_0143) is essentia...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.02472-14

    authors: Bian J,Tu Y,Wang SM,Wang XY,Li C

    更新日期:2015-04-01 00:00:00

  • Alterations in the cytoplasmic membrane proteins of various chlorate-resistant mutants of Escherichia coli.

    abstract::Chlorate-resistant mutants corresponding to each known genetic locus (chlA, chlB, chlC, chlD, chlE) were isolated from Escherichia coli K-12. All these mutants showed decreased amounts of membrane-bound nitrate reductase, cytochrome b, and formic dehydrogenase, but all had normal succinic dehydrogenase activity. Prote...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.108.1.564-570.1971

    authors: MacGregor CH,Schnaitman CA

    更新日期:1971-10-01 00:00:00

  • Glycosylation of the self-recognizing Escherichia coli Ag43 autotransporter protein.

    abstract::Glycosylation is a common modulation of protein function in eukaryotes and is biologically important. However, in bacteria protein glycosylation is rare, and relatively few bacterial glycoproteins are known. In Escherichia coli only two glycoproteins have been described to date. Here we introduce a novel member to thi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.188.5.1798-1807.2006

    authors: Sherlock O,Dobrindt U,Jensen JB,Munk Vejborg R,Klemm P

    更新日期:2006-03-01 00:00:00

  • lpt6, a gene required for addition of phosphoethanolamine to inner-core lipopolysaccharide of Neisseria meningitidis and Haemophilus influenzae.

    abstract::We previously described a gene, lpt3, required for the addition of phosphoethanolamine (PEtn) at the 3 position on the beta-chain heptose (HepII) of the inner-core Neisseria meningitidis lipopolysaccharide (LPS), but it has long been recognized that the inner-core LPS of some strains possesses PEtn at the 6 position (...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.186.20.6970-6982.2004

    authors: Wright JC,Hood DW,Randle GA,Makepeace K,Cox AD,Li J,Chalmers R,Richards JC,Moxon ER

    更新日期:2004-10-01 00:00:00

  • tRNA Modification Profiles and Codon-Decoding Strategies in Methanocaldococcus jannaschii.

    abstract::tRNAs play a critical role in mRNA decoding, and posttranscriptional modifications within tRNAs drive decoding efficiency and accuracy. The types and positions of tRNA modifications in model bacteria have been extensively studied, and tRNA modifications in a few eukaryotic organisms have also been characterized and lo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00690-18

    authors: Yu N,Jora M,Solivio B,Thakur P,Acevedo-Rocha CG,Randau L,de Crécy-Lagard V,Addepalli B,Limbach PA

    更新日期:2019-04-09 00:00:00

  • PATHWAYS OF GLUCOSE CATABOLISM IN BACILLUS CEREUS.

    abstract::Goldman, Manuel (The University of Michigan, Ann Arbor), and Harold J. Blumenthal. Pathways of glucose catabolism in Bacillus cereus. J. Bacteriol. 87:377-386. 1964.-Estimates by a radiorespirometric method of the pathways of glucose catabolism of resting-cell suspensions of Bacillus cereus strain terminalis indicate ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.87.2.377-386.1964

    authors: GOLDMAN M,BLUMENTHAL HJ

    更新日期:1964-02-01 00:00:00

  • Changes in Escherichia coli rRNA promoter activity correlate with changes in initiating nucleoside triphosphate and guanosine 5' diphosphate 3'-diphosphate concentrations after induction of feedback control of ribosome synthesis.

    abstract::rRNA synthesis is the rate-limiting step in ribosome synthesis in Escherichia coli. Its regulation has been described in terms of a negative-feedback control loop in which rRNA promoter activity responds to the amount of translation. The feedback nature of this control system was demonstrated previously by artificiall...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.20.6185-6191.2003

    authors: Schneider DA,Gourse RL

    更新日期:2003-10-01 00:00:00

  • The Tn10-encoded tetracycline resistance mRNA contains a translational silencer in the 5' nontranslated region.

    abstract::We performed a mutational analysis of the left half of Tn10-encoded tet operator O2, located in the 5' nontranslated region of the mRNA for the resistance protein TetA, and determined the importance of that region for translation efficiency and mRNA stability. Transcriptional fusions of 17 mutants to lacZ expressed th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.8.2478-2484.1992

    authors: Flache P,Baumeister R,Hillen W

    更新日期:1992-04-01 00:00:00

  • Intraperiplasmic growth of Bdellovibrio bacteriovorus 109J: N-deacetylation of Escherichia coli peptidoglycan amino sugars.

    abstract::During intraperiplasmic growth of Bdellovibrio bacteriovorus on Escherichia coli, the substrate cell peptidoglycan is extensively modified as it is converted to bdelloplast peptidoglycan. The initially lysozyme-sensitive peptidoglycan of E. coli was rapidly converted to a lysozyme-resistant form. The conversion was du...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.135.3.1008-1014.1978

    authors: Thomashow MF,Rittenberg SC

    更新日期:1978-09-01 00:00:00

  • Streptococcus pneumoniae uses glutathione to defend against oxidative stress and metal ion toxicity.

    abstract::The thiol-containing tripeptide glutathione is an important cellular constituent of many eukaryotic and prokaryotic cells. In addition to its disulfide reductase activity, glutathione is known to protect cells from many forms of physiological stress. This report represents the first investigation into the role of glut...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01393-12

    authors: Potter AJ,Trappetti C,Paton JC

    更新日期:2012-11-01 00:00:00

  • The Bacillus subtilis nucleoid-associated protein HPB12 strongly compacts DNA.

    abstract::The HPB12 protein from the nucleoid of Bacillus subtilis was previously described, and its DNA binding properties have been reported previously (V. Salti, F. Le Hégarat, and L. Hirschbein, Biochim. Biophys. Acta 1009:161-167, 1989). The DNA-HPB12 complexes were examined by electron microscopy. They appeared as short, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.1.50-60.1994

    authors: Arnold-Schulz-Gahmen B,Salti-Montesanto V,Nguyen J,Hirschbein L,Le Hégarat F

    更新日期:1994-01-01 00:00:00

  • Electron microscopy of Bacillus megaterium undergoing isolation of its nuclear bodies.

    abstract::Fitz-James, Philip (University of Western Ontario, London, Canada). Electron microscopy of Bacillus megaterium undergoing isolation of its nucelar bodies. J. Bacteriol. 87:1202-1210. 1964.-The various steps of treatment leading to the isolation of nuclear bodies were followed by thin-section electron microscopy. Nucle...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.87.5.1202-1210.1964

    authors: Fitz-James P

    更新日期:1964-05-01 00:00:00

  • Cloning and characterization of two Serratia marcescens genes involved in core lipopolysaccharide biosynthesis.

    abstract::Bacteriocin 28b from Serratia marcescens binds to Escherichia coli outer membrane proteins OmpA and OmpF and to lipopolysaccharide (LPS) core (J. Enfedaque, S. Ferrer, J. F. Guasch, J. Tomás, and M. Requé, Can. J. Microbiol. 42:19-26, 1996). A cosmid-based genomic library of S. marcescens was introduced into E. coli N...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.19.5741-5747.1996

    authors: Guasch JF,Piqué N,Climent N,Ferrer S,Merino S,Rubires X,Tomas JM,Regué M

    更新日期:1996-10-01 00:00:00

  • Characterization of the gene encoding glutamine synthetase I (glnA) from Bradyrhizobium japonicum.

    abstract::We have isolated the Bradyrhizobium japonicum gene encoding glutamine synthetase I (glnA) from a phage lambda library by using a fragment of the Escherichia coli glnA gene as a hybridization probe. The rhizobial glnA gene has homology to the E. coli glnA gene throughout the entire length of the gene and can complement...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.162.2.698-703.1985

    authors: Carlson TA,Guerinot ML,Chelm BK

    更新日期:1985-05-01 00:00:00

  • Genetic and molecular characteristics of Vir plasmids of bovine septicemic Escherichia coli.

    abstract::Three wild strains of bovine septicemic Escherichia coli were selected on the basis of their production of a toxin lethal for mice and chickens and their characteristic surface antigen. The transfer of these virulence (Vir) properties from two of the three to recipient E. coli was detected after mating. One Vir plasmi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.141.2.758-769.1980

    authors: Lopez-Alvarez J,Gyles CL,Shipley PL,Falkow S

    更新日期:1980-02-01 00:00:00

  • Phenol: a complex chemoeffector in bacterial chemotaxis.

    abstract::Earlier observations that phenol is a repellent for Salmonella typhimurium but an attractant for Escherichia coli were confirmed. This behavioral difference was found to correlate with a difference in the effect phenol had on receptor methylation levels; it caused net demethylation in S. typhimurium but net methylatio...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.1.371-379.1987

    authors: Imae Y,Oosawa K,Mizuno T,Kihara M,Macnab RM

    更新日期:1987-01-01 00:00:00

  • Adaptation of Xanthobacter autotrophicus GJ10 to bromoacetate due to activation and mobilization of the haloacetate dehalogenase gene by insertion element IS1247.

    abstract::Monobromoacetate (MBA) is toxic for the 1,2-dichloroethane-degrading bacterium Xanthobacter autotrophicus GJ10 at concentrations higher than 5 mM. Mutants which are able to grow on higher concentrations of MBA were isolated and found to overexpress haloacid dehalogenase, which is encoded by the dhlB gene. In mutant GJ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.5.1348-1356.1995

    authors: van der Ploeg J,Willemsen M,van Hall G,Janssen DB

    更新日期:1995-03-01 00:00:00

  • A ction of cycloheximide on Zygosaccharomyces soja.

    abstract::Tsukada, Yoji (Kyoto University, Kyoto, Japan), Tsunetake Sugimori, Kazutami Imai, and Hideo Katagiri. Action of cycloheximide on Zygosaccharomyces soja. J. Bacteriol. 83:70-75. 1962.-Cycloheximide is known to inhibit the growth of some species of Saccharomyces and other fungi. A new type of action of this antibiotic,...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.83.1.70-75.1962

    authors: TSUKADA Y,SUGIMORI T,IMAI K,KATAGIRI H

    更新日期:1962-01-01 00:00:00

  • Cloning and analysis of the Bacillus subtilis rpsD gene, encoding ribosomal protein S4.

    abstract::The rpsD gene, encoding ribosomal protein S4, was isolated from Bacillus subtilis by hybridization with oligonucleotide probes derived from the S4 amino-terminal protein sequence. Sequence analysis of the cloned DNA indicated that rpsD is likely to be monocistronic, in contrast to Escherichia coli rpsD, which is locat...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.11.6372-6379.1990

    authors: Grundy FJ,Henkin TM

    更新日期:1990-11-01 00:00:00

  • Complete genome sequences of Desulfosporosinus orientis DSM765T, Desulfosporosinus youngiae DSM17734T, Desulfosporosinus meridiei DSM13257T, and Desulfosporosinus acidiphilus DSM22704T.

    abstract::Desulfosporosinus species are sulfate-reducing bacteria belonging to the Firmicutes. Their genomes will give insights into the genetic repertoire and evolution of sulfate reducers typically thriving in terrestrial environments and able to degrade toluene (Desulfosporosinus youngiae), to reduce Fe(III) (Desulfosporosin...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01392-12

    authors: Pester M,Brambilla E,Alazard D,Rattei T,Weinmaier T,Han J,Lucas S,Lapidus A,Cheng JF,Goodwin L,Pitluck S,Peters L,Ovchinnikova G,Teshima H,Detter JC,Han CS,Tapia R,Land ML,Hauser L,Kyrpides NC,Ivanova NN,Pagani

    更新日期:2012-11-01 00:00:00

  • Factors Affecting the Survival of Auxotrophs and Prototrophs of Saccharomyces cerevisiae in Mixed Populations.

    abstract::Moat, Albert G. (Hahnemann Medical College, Philadelphia, Pa.), Isabel J. Barnes, and Eleanor H. McCurley. Factors affecting the survival of auxotrophs and prototrophs of Saccharomyces cerevisiae in mixed populations. J. Bacteriol. 92:297-301. 1966.-The conditions under which the number of yeast prototrophs, as well a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.92.2.297-301.1966

    authors: Moat AG,Barnes IJ,McCurley EH

    更新日期:1966-08-01 00:00:00

  • Physiological studies of the regulation of beta-lactamase expression in Pseudomonas maltophilia.

    abstract::The kinetics of beta-lactamase induction in Pseudomonas maltophilia IID1275/873 were investigated. Upon induction with beta-lactam antibiotics, a correlation was seen between the increase in specific beta-lactamase activity and the generation time, as well as the concentration of inducer in the medium. The specific be...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.1.483-487.1989

    authors: Rosta S,Mett H

    更新日期:1989-01-01 00:00:00

  • Functional reconstitution of SdcS, a Na+-coupled dicarboxylate carrier protein from Staphylococcus aureus.

    abstract::In Staphylococcus aureus, the transport of dicarboxylates is mediated in part by the Na+-linked carrier protein SdcS. This transporter is a member of the divalent-anion/Na+ symporter (DASS) family, a group that includes the mammalian Na+/dicarboxylate cotransporters NaDC1 and NaDC3. In earlier work, we cloned and expr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01452-06

    authors: Hall JA,Pajor AM

    更新日期:2007-02-01 00:00:00

  • Effects of the Calvin cycle on nicotinamide adenine dinucleotide concentrations and redox balances of Xanthobacter flavus.

    abstract::The levels of reduced and oxidized nicotinamide adenine dinucleotides were determined in Xanthobacter flavus during a transition from heterotrophic to autotrophic growth. Excess reducing equivalents are rapidly dissipated following induction of the Calvin cycle, indicating that the Calvin cycle serves as a sink for ex...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.16.4637-4639.2000

    authors: van Keulen G,Dijkhuizen L,Meijer WG

    更新日期:2000-08-01 00:00:00

  • Thermoregulation of virB transcription in Shigella flexneri by sensing of changes in local DNA superhelicity.

    abstract::Transcription of the virB gene, a transcriptional regulator of invasion genes on the large plasmid of Shigella flexneri, is strictly regulated by growth temperature; when bacteria are grown at 37 degrees C, virB transcription is highly activated, while at 30 degrees C the level of virB transcription decreases to less ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.4.1094-1097.1995

    authors: Tobe T,Yoshikawa M,Sasakawa C

    更新日期:1995-02-01 00:00:00

  • Impaired expression of certain prereplicative bacteriophage T4 genes explains impaired T4 DNA synthesis in Escherichia coli rho (nusD) mutants.

    abstract::The Escherichia coli rho 026 mutation that alters the transcription termination protein Rho prevents growth of wild-type bacteriophage T4. Among the consequences of this mutation are delayed and reduced T4 DNA replication. We show that these defects can be explained by defective synthesis of certain T4 replication-rec...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.7.3872-3880.1989

    authors: Stitt BL,Mosig G

    更新日期:1989-07-01 00:00:00

  • Multiple mechanisms are used for growth rate and stringent control of leuV transcriptional initiation in Escherichia coli.

    abstract::Expression of the Escherichia coli leuV operon, which contains three tRNA(1)(Leu) genes, is regulated by several mechanisms including growth-rate-dependent control (GRDC) and stringent control (SC). Structural variants of the leuV promoter which differentially affect these regulatory responses have been identified, su...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.18.5771-5782.1999

    authors: Pokholok DK,Redlak M,Turnbough CL Jr,Dylla S,Holmes WM

    更新日期:1999-09-01 00:00:00

  • Isolation, sequencing, and mutagenesis of the gene encoding NAD- and glutathione-dependent formaldehyde dehydrogenase (GD-FALDH) from Paracoccus denitrificans, in which GD-FALDH is essential for methylotrophic growth.

    abstract::NAD- and glutathione-dependent formaldehyde dehydrogenase (GD-FALDH) of Paracoccus denitrificans has been purified as a tetramer with a relative molecular mass of 150 kDa. The gene encoding GD-FALDH (flhA) has been isolated, sequenced, and mutated by insertion of a kanamycin resistance gene. The mutant strain is not a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.1.247-251.1995

    authors: Ras J,Van Ophem PW,Reijnders WN,Van Spanning RJ,Duine JA,Stouthamer AH,Harms N

    更新日期:1995-01-01 00:00:00

  • RNA arbitrarily primed PCR survey of genes regulated by ToxR in the deep-sea bacterium Photobacterium profundum strain SS9.

    abstract::We are currently investigating the role of ToxR-mediated gene regulation in Photobacterium profundum strain SS9. SS9 is a moderately piezophilic ("pressure loving") psychrotolerant marine bacterium belonging to the family Vibrionaceae. In Vibrio cholerae, ToxR is a transmembrane DNA binding protein involved in mediati...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.5.1688-1693.2001

    authors: Bidle KA,Bartlett DH

    更新日期:2001-03-01 00:00:00