Events following prophage Mu induction.

Abstract:

:Escherichia coli strains lysogenic for a thermoinducible Mu prophage (Mu cts62) undergo rapid lysis about 50 min after heat induction. Induction of Mu cts62 apparently causes damage to the host sequences in which Mu is inserted. The normal expression of A, BU, and X genes of Mu is needed for this specific deleterious effect on the prophage-containing host sequences. Mu deoxyribonucleic acid can be shown to reintegrate extensively at different sites on the host genome during the lytic cycle after prophage induction or after infection of sensitive cells by clear-plaque mutants of Mu. We estimate that approximately 10 copies of Mu deoxyribonucleic acid are inserted per chromosome during vegetative growth. The episome rescue method for detecting vegetative Mu deoxyribonucleic acid insertion, in which an episome is transferred from the lytically infected cells to F- receipient cells, can be applied to study Mu integration without requiring the host cells to survive. It also provides an easy system to isolate Mu insertions in transmissible episomes and plasmids.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Razzaki T,Bukhari AI

doi

10.1128/JB.122.2.437-442.1975

keywords:

subject

Has Abstract

pub_date

1975-05-01 00:00:00

pages

437-42

issue

2

eissn

0021-9193

issn

1098-5530

journal_volume

122

pub_type

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